Agilent Highly sulphonated cyclodextrins for chiral analysis
Contents
1. A ii s n 2 5 20 2 10 0 T T T T nr a a T 0 3 0 9 Voltage kV 0 31 S A 100 Figure 2 Ohm s law plot for separation conditions 50 0 2 4 6 8 10 12 14 The use of highly sulphonated ee J Figure 3 cyclodextrins in conjunction with 25 um id extended light path capillaries provides a reproducible and sensitive method for separating chiral compounds This combination also provides a method development versatility in choice of temperature and cyclodex trin concentration which is not easily achieved with wider bore capillaries Clenbuterol Migration time reproducibility and sensitivity www agilent com chem ce Agilent Technologies Inc 1998 2008 Published November 1 2008 Publication Number 5989 9810EN 2 2 Agilent Technologies2. Author Gordon Ross Agilent Technologies Cheadle United Kingdom Highly sulphonated cyclodextrins for chiral analysis Application Note Pharmaceutical Abstract Capillary electrophoresis is ideally suited to the analysis of chiral solutes where the ease of method development and greatly reduced costs makes it an attractive alternative to liquid chromatography The use of highly sulphonated cyclodextrins has been described together with their more generic application to separate a range of analytes Highly sulphonated cyclodextrins have an associated high current which must be controlled in order that Joule heating does not unduly deteriorate the separation This Application Note describes the use of highly sulphonated cyclodextrins for the analysis of chiral analytes By using small 25 um id capillaries with an extended pathlength of 125 um id the Joule heating can be controlled while enhancing sensitivity ORE Agilent Technologies Experimental All experiments were performed using the Agilent Capillary Electrophoresis system equipped with diode array detection and the Agilent ChemStation software Heptakis 6 sulfato R cyclodextrin was supplied by Regis Technologies Inc Illinois USA The small bore capillary was quickly and easily flushed using a high pressure of 8 to 12 bar Negative polarity is employed since the enantiomers are carried towards the detection end by their interaction with the cyclodextrins Fig
3. ure 1 shows the generic applicability of the cyclodextrins by applying one method and set of conditions to a range of chiral solutes The excellent control of Joule heating is demonstrated by the linearity of Ohm s Law plot V vs figure 2 This conforms almost per fectly to theoretical expectations The stability of thermostatting is further demonstrated by the reproducibility of the peak migration times of the clen buterol enantiomers at 0 30 peak 1 and 0 31 peak 2 for n 8 figure 3 The linearity of detection was also determined over the range 0 03 to 5 mg mL with r 0 999 while the concentration limit of detection at a signal to noise ratio of ca 2 5 was 30 pg mL figure 3 Equipment e Agilent Capillary Electrophoresis system e Agilent ChemStation e Extended light path capillaries a gt 3 5 DOMA _Doxylamine Absorbance _ Isoproterenol mAU Epinephrine 1200 1000 800 Methyl dopa 600 Cromakalim Clenbuterol Time scale K min 0 5 N A Figure 1 Separation of several drugs using a single method Chromatographic conditions Capillary Effective length 40 cm total length 48 5 cm internal diameter 25 um BF 5 Agilent part number G1600 60132 Buffer 25 mM phosphate TEA pH 3 3 5 w v Heptakis sulpho R cyclodextrin Detection 195 10 nm Injection 500 mbar x s Voltage 30 kV Temperature 25 C a 4 N Absorbance Current mAU u
Download Pdf Manuals
Related Search