Agilent Cloning PCR Combo Pack Product Guide
Contents
1. Q How long can store the components of the combo pack A Each kit component within the combo pack has its own expiration date Agilent will guarantee products within the kit for 12 months from date of purchase when stored as instructed Q What other cloning products are available from Agilent A Agilent offers a variety of PCR enzymes cloning vectors and library construction kits Visit www agilent com genomics cloning for the full line of product options Cloning PCR Combo Pack Product Descriptions and Catalog Reorder Information Product Description Catalog StrataPrep Plasmid MiniPrep Kit Reagents and materials needed for rapid phenol free 400761 50 preps purification of plasmid DNA from bacterial cultures 400763 200 preps DNA Extraction Kit Reagents for obtaining highly pure DNA from tissues 200600 cell culture pellets or blood StrataPrep DNA Gel Extraction Kit Reagents and materials needed for rapid high yield 400766 50 extractions isolation of DNA from agarose 400768 250 extractions StrataClone PCR Cloning Kit Reagents for performing 5 minute cloning of PCR 240205 20 reactions products using a topoisomerase based method Easy A High Fidelity PCR Cloning DNA polymerase blend for high fidelity PCR 600400 100 U Enzyme amplification of cloning inserts with 3 overhangs 600402 500 U 600404 1000 U Deoxynucleotide Mix PCR grade 100 mM dNTP mix 25 mM of each dNTP 200415 4002. the Deoxynucleotide Mix for PCR amplification of the clone insert Setup the PCR reactions in the supplied Tube Strips and cap the tubes with the supplied Tube Cap Strips The Tube Strip Capping Tool helps ensure tubes are properly sealed PCR Amplity Cloning Insert Use the StrataCooler LP Benchtop Cooler to keep your reactions and reagents sufficiently cold during set up Analyze PCR Use the Kb DNA Ladder to check your PCR product size relative yield Products and purity on an agarose gel Purify PCR Product Use the StrataPrep DNA Gel Extraction Kit to gel purify the insert or use the StrataPrep PCR Purification Kitto purify the insert Clone Insert into without gel purification The insert may then be cloned into your l StrataClone ownvector OR Clone Insert Use the StrataClone PCR Cloning Kit to prepare the insert ligate into the StrataClone vector and transform competent cells into Vector S Use the StrataPrep MiniPrep Kit to isolate the recombinant plasmid from competent cells Vector Purify pDNA Agilent Cloning PCR Combo Pack Product Guide page 3 Cloning PCR Combo Pack FAQ s Q The combo pack components did not get used up at the same time Why A The kits included in the combo pack do not provide for the same number of experiments and usage will vary depending on your specific experimental needs Please refer to page 4 for reordering information on individual kits products
3. ul Kb DNA Ladder Mix of 15 DNA fragments spanning 250 bp to 12 kb 201115 250 ug Tube Strips Thin walled 200 ul PCR reaction tubes in strips of 12 410082 80 strips Tube Cap Strips Caps for PCR reaction tubes in strips of 12 410086 80 strips Tube Strip Capping Tool Hand tool for pressing down cap strips on tube strips 410099 2 tools StrataCooler LP Benchtop Cooler Cooler with slots for holding 1 5 ml and 0 5 ml tubes to 401349 1 cooler keep temperature sensitive enzymes cold while working at the bench Contact Us Find an Agilent customer center in your country www agilent com genomics contactus Email Technical Services techservices agilent com Agilent Cloning PCR Combo Pack Product Guide page 4
4. hte Agilent Technologies Agilent Cloning PCR Combo Pack Product Guide Catalog 929900 PACKING LisT BY BOX NUMBER Box 1 of 4 20 Components StrataClone PCR Cloning Kit PCR components Vector Mix amp kan Cloning Buffer and Control Insert DNA Extraction Kit Dry ice components RNase and Pronase Easy A High Fidelity PCR Cloning Enzyme Deoxynucleotide Mix Kb DNA Ladder Product Guide Cloning PCR Combo Pack Box 2 of 4 80 C Components StrataClone PCR Cloning Kit SoloPack components SoloPack Competent Cells and pUC18 Control Plasmid Product Guide Cloning PCR Combo Pack Box 3 of 4 Room Temp Components 1 DNA Extraction Kit Room temperature components Solution 1 Solution 2 and Solution 3 StrataCooler LP Benchtop Cooler Tube Strips Tube Cap Strips Tube Strip Capping Tool Contact Agilent Magnet Product Guide Cloning PCR Combo Pack Box 4 of 4 Room Temp Components 2 StrataPrep Plasmid MiniPrep Kit StrataPrep DNA Gel Extraction Kit Product Guide Cloning PCR Combo Pack INTRODUCTION The Cloning PCR Combo Pack provides kits reagents and materials to rapidly and efficiently clone your PCR products For additional cloning related reagents please see Agilent s full line of products at www agilent com genomics The list of components on page 2 Itemizes the individual products that are included in the combo pack The product workflow on page 3 explains how the components of the combo pack are used t
5. ogether in a PCR cloning application PRODUCT MANUALS To download the instruction manuals needed to use the products in the combo pack visit the websites listed below StrataPrep Plasmid MiniPrep Kit www genomics agilent com files Manual 400761 pdf DNA Extraction Kit www genomics agilent com files Manual 200600 pdf StrataPrep DNA Gel Extraction Kit www genomics agilent com files Manual 400766 pdf Easy A High Fidelity PCR Cloning Enzyme www genomics agilent com files Manual 600400 pdf StrataClone PCR Cloning Kit www genomics agilent com files Manual 240205 pdf Agilent Cloning PCR Combo Pack Product Guide 929900 13 revision A Agilent Technologies Inc 2011 StrataPrep Plasmid MiniPrep Kit 50 preps Part Number Material 400761 13 Solution 1 6 ml 400761 14 Solution 2 6 ml 400761 15 Solution 3 8 ml 400761 16 Wash Buffer 2x 25 ml 400761 17 Nuclease removal buffer 1x 25 ml 400761 110 Microspin cups amp receptacle tubes 50 each StrataClone PCR Cloning Kit 20 rxns Part Number Material 240205 51 StrataClone vector mix amp kan 21 ul 240205 54 StrataClone cloning buffer 63 ul 240205 53 StrataClone control insert 10 ul 200185 41 StrataClone SoloPack competent cells 1 05 ml 200231 42 pUC18 control plasmid 10 ul Easy A High Fidelity PCR Cloning Enzyme 40 x 50 ul rxns Part Number Material 600400 51 Easy A high fidelity PCR cloning enzyme 5 U ul 600400 52 10x Easy A reaction buffer Kb DNA Ladder 15 fragment
6. s 250 bp 12 kb Part Number Material 201115 81 Kb DNA ladder 500 ul Tube Strips Part Number Material 410082 0 2 ml Tube strips 80 strips of 12 Tube Strip Capping Tool Part Number Material 410099 Tube strip capping tools 2 each COMPONENTS OF THE CLONING PCR Comeo Pack CataLoG 929900 DNA Extraction Kit 30 35 extractions PartNumber Material 200600 13 Solution 1 3x 500 ml 200600 14 Solution 2 420 ml 200600 15 Solution 3 150 ml 200600 81 RNase 1 ml 200600 82 Pronase 2 ml StrataPrep DNA Gel Extraction Kit 50 preps PartNumber Material 400766 13 DNA extraction buffer 20 ml 400761 16 Wash buffer 2x 25 ml 211189 110 Microspin cups amp receptacle tubes 50 each Deoxynucleotide Mix 1000 rxns Material 100 mM dNTP Mix 400 ul Part Number 200415 51 StrataCooler LP Benchtop Cooler Part Number Material 401349 StrataCooler LP benchtop cooler 1 each Tube Cap Strips Part Number Material 410086 Tube cap strips 80 strips of 12 Note Pack sizes vary for each individual kit in the combo pack Catalog information for reordering individual components is listed on page 4 of this guide Agilent Cloning PCR Combo Pack Product Guide page 2 Cloning PCR Combo Pack Product Workflow Use the DNA Extraction Kit to extract genomic DNA from cells or tissues or Prepare DNA usethe StrataPrep MiniPrep Kitto isolate plasmid DNA from E coli Use the Easy A High Fidelity PCR Cloning Enzyme and
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