胰岛素样生长因子结合 protein 3 quantify 测定 kit ( elisa immune 法) Manual
Contents
1. TMB IGFBP 3 405 nm 650 nm 1 101 IDS IDS IGFBP 3 ELISA2. 134 IGFBP 3 225 4035 ng mL IDS 0 41 x 122 95 CI 0 37 0 45 66 309 r 0 85 NCCLS EP 5A2 3 37 58 ng mL sD CV sD CV 1 60 1433 49 3 4 122 8 5 2 60 4904 280 57 544 11 1 3 60 6738 400 5 9 785 11 7 20 2 137 ng mL 0 IGFBP 3 NCCLS EP 6A
3. 8X12 0 05 1 mL 2 AC STOP TMB 24 mL WASHBUH Po REF Ac WASHL I 24 mL 12 mL 4 4 MICROPLAT 8 1 2 3 1 CAL CTRL 10 15
4. mL mL 2 ME ng mL 1566 1621 55 4 2009 2163 154 8 2451 2476 25 1 2894 2784 110 4 3336 3119 217 7 3779 3832 54 1 4221 4012 209 5 4664 4670 6 0 5106 5349 243 5 Oberved value ng ml 6000 5000 4000 High Sample IGFBP 3 ng mL IGF 1 ng mL ng mL ng mL 1072 1000 1955 883 88 1025 1000 2000 975 97 1178 1000 2164 986 99 99 IGFBP 3 100 Insulin 2 500IU mL ND IGF I 100ug mL ND IGF Il 4ug mL ND IGFBP 1 10ug mL ND IGFBP 2 10ug mL ND IGFBP 4 10ug mL ND IGFBP 5 10ug mL ND IGFBP 6 10ug mL ND IDS CTRL FDA HIV 1 amp 1 amp HBV amp HCV
5. 95 ng mL ng mL 0 2 7 517 365 1294 3 4 4 1915 1366 2632 5 6 9 1257 878 2026 7 8 5 1803 1416 2574 9 10 27 1750 1089 3209 11 12 27 2423 1149 3117 13 14 45 2285 921 3222 15 16 47 2155 1569 3000 17 18 54 2116 1110 2857 19 20 49 2161 1513 3578 21 30 45 2681 1627 5619 31 40 44 3001 1700 5205 41 50 75 2481 1284 4330 51 60 173 2335 1448 3567 61 70 43 2201 1195 3714 71 80 5 1673 1094 3177 IGFBP 3 IDS 3 my MultiCalc PerkinElmer VS Log lin log
6. 5 100 pL AB BIOTIN 6 500 700rpm 18 25 C 2 7 a 300 pL 3 b 250 pL 3 8 200 ML ENZYMCONJ 2 9 10 7 18 25 C 2 30 TMB 12 100 HL HCLU 13 30 IGFBP 3 18 25 C 2 30
7. 3 IGF IGFBPs 6 IGFBP 3 GH IDS IGFBP 3 ELISA IGFBP 3 IGFBP 3 3 IGFBP 3 IGFBP 3 mal 1 CAL 1 C CAL BSA 0 05 50 mL AC 1901B AC 1901F CAL 15 HCL MB AB BIOTIN GFBP 3 IGFBP 3
8. ng mL linear linear HE YEZ Absorbance 200 10000 ng mL 200 ng mL 200 ng mL U L A1 A2 0 0 058 0 060 0 ng mL 0 062 B1 B2 1 0 113 0 113 200 ng mL 0 113 C1 C2 2 0 222 0 217 500 ng mL 0 211 D1 D2 3 0 842 0 825 2000 ng mL 0 807 E1 E2 4 1 781 1 755 5000 ng mL 1 729 F1 F2 5 2 612 2 556 1 0000 ng mL 2 500 G1 G2 1 0 536 0 525 1259 0 514 H1 H2 2 1 645 1 631 4499 1 617 2 5 2 0 15 1 0 0 5 0 0 100 10000 IGFBP 3 ng mL 1 Calibrator 0
9. CTRL 20 C 2 8 C 4 20 C 1 mL 1 CTRU E CTRL 2 CTRU 10 pL 3 1 0 mL 0 CAL 0 4 50 pL CALU CTRU MICROPLAT 10
10. CTRL 20 C 30 15 E WASHBUF 20x NO OOD 12X75mm 10bL 50HL J 1mL 100pL 200pL Eppendorf Multipipette 4780 2 8 C 950 mL 450nm 650nm
11. IDS IGFBP 3 ELISA 2 6 TMB FMBI 3 3 5 5 R21 22 S36 37 0 5M HCL
12. IDS IGFBP 3 ELISA IGF I IGF ID IGFBP 1 6 IGFBPs IGF IGFBPs IGFBP 3 nonglycosylated MW 29kDA REF REF IGFBP 3 BSA 0 05 MICROPLAT REF AC 1902W 3 I 3 IGF AC 1901A REF Ac 1903 ENZYMCONJ 0 5M 14 mL TMB REF AcC TMB REF 4 CTRU 1 2
13. 2 3 IGFBP 3 4 5 FE LA NCCLS EP7 A 4 500 mg dL 20 mg dL EE 3000 mg dL 800 mg dL EE Se IGFBP 3 6 IGFBP 3 IDS IGFBP 3 ELISA IGFBP 3 ELISA NCCLS EP 9A2 800 mg dL 50 000 ng mL
14. IREF AC 1905A AC 1905B IGFBP 3 REF 10 50 mL i FE IGF IGF gt 75 E 4 E GFBP 3 AC 1904 A TH 1 mL 5j lt IGFBP 3 GH GH IGFBP 3 GH gt 95 IGF I IGF II IGFS IGFBP 3 264 MW 4 mT
15. 0 5M R36 38 S26 i E S36 37 1 Badaru A Wilson D Alternatives to growth hormone stimulation testing in children Trends Endocrinol Metab 2004 15 252 258 2 Bray Gunnell D Holly JM et al Associations of childhood and adulthood height and the components of height with insulin like growth factor IGF levels in adulthood 65 year follow up of the Boyd Orr cohort J Clin Endocrinol Metab 2006 91 1382 1389 3 Cianfarani S Liguori A Germani D IGF l and IGFBP 3 in the management of childhood onset growth hormone defi ciency Endocr Dev 2005 9 66 75 4 Hasegawa Y Hasegawa T Anzo M et al Clinical utility of insulin like growth factor IGF I and IGF binding protein 3 levels in the diagnosis of GH defi ciency GHD during childhood Endocr J 1996 43 Suppl S1 S4 5 Jensen RB Jeppsen KA Vielwerth S et al Insulinlike growth factor IGF l and IGF binding protein 3 as diagnostic markers of growth hormone defi ciency in infancy Horm Res 2005 63 15 21 6 Lofqvist C Andersson E Gelander L et al Reference values for insulin like growth factorbinding protein 3 IGFBP 3 and the ratio of insulin like growth factor to IGFBP 3 throughout childhood and adolescence J Clin E
16. ndocrinol Metab 2005 90 1420 1427 Inmunodiagnostic Systems Linited IDS Ltd 10 Didcot Way Boldon Business Park Boldon Tyne amp amp Wear NE35 9PD UK 44 0 191 519 0660 44 0 191 519 0760 www idsltd com 88 12 B12 86 10 58895646 86 20 32293178 86 10 58895611 86 20 32293177 info rz biotech com www rz biotech com 7
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