Agilent 固相抽出とLC/MS/MS による毛髪中のコカイン ベンゾイルエクゴニン コカエチレン ノルコカインの同定 Manual
Contents
1. Agilent Technologies Inc 2008 Printed in Japan August 31 2008 5989 7212JAJP Agilent Technologies2. 50 pg mg 25 pg mg n 5 1 3 8 1 0 8 0 4 n 10 4 8 9 2 15 7 12 6 COC 50 pg mg GC MS 1 2 CE NC CE NC
3. 8 LOQ 0 2 S N 10 LOD S N 3 100 pg mg 5 5 10 1 2 5 n 10 50 pg mg 48 4 C
4. LC MS LC Agilent 1200 Agilent Z0RBAX XDB C18 4 6 x 50 mm 1 8 nm 922975 902 40 C A 20 mM pH 6 4 B 0 9 mL min 2 HL 1 MRM 1 0 290 3 gt 168 3 105 3 D3 293 3 gt 171 4 2 3 2 3 4 304 3 gt 182 3 82 2 D3 307 3 gt 185 3 4 4 9 318 3 gt 196 4 82 2 D8 326 3 gt 204 4 290 3 gt 168 3 136 3 D3 293 3 gt 171 4 B mL min 0 0 1 5 30 0 9 4 5 55 1 5 60 1 7 7 75 1 6 75 25
5. n 3 0 9989 y 0 00116x 6 7 10 0 9995 y 0 00106x 37 8 56 8 0 9987 y 0 00061x 49 3 74 0 9992 y 0 00096x 52 8 79 2 NRN 4 50 pg mg I BZE COC CE NC 9 2 4 8 15 7 12 6 n 10 n 5 8 1 1 3 0 8 4 7 C
6. 50 nL 0 025 M pH 2 7 1 5 mL 75 C 2 0 1 M 10 Clin IT 691 0353T 78 20 2 YY 2 mL 2 mL 2 ml 0 1 M 1 mL 2 mL 0 1 M 2 mL 2 mL 2 mL 30 psi 2
7. 20 1 FE S Romolo M C Rotolo 1 Palmi R Pacifici A Lopez Forens SC Int 138 103 2003 17 2 P Kintz P Mangin Forens Sc Int 73 2 1995 93 3 E Cognard S Rudaz S Bouchonnet C Staub J Chromatogr B 826 2005 17 4 J A Bourland E F Hayes R C Kelly S A Sweeney M M Hatab J Anal Toxicol 24 7 2000 489 5 M Klys S Rojek J Kulikowska E Bozek M Scislowski J Chromatogr B 854 2007 299 6 K B Scheidweiler M H Huestis Anal Chem 76 2004 4358 7 B Maralikova W Weinmann J Chromatogr B 811 2004 21 8 S S Johansen H M Bhatia J Chromatogr B 852 1 2 2007 338 www agilent com chem jp www adgilent com chem jp
8. R2 gt 0 99 CE 3 2 BZRE 2 6 7 10 53 54 55 56 Acquisitlon Time min Calibration Curve ls 3 Type Linear i OIGINN Ianore y Weight None 1672E 004 0 0042 BI 2 10 3301645 50 2 500 pg mg R gt 0 999 Relative Hesponses Origins Ionore Weighti None mlitstD Cocaethylene 6 Levek 6 Levels ed 6 Points 6 Points Used 0 QCs no 1 ED R 2 099901645 1 3 1 2 3 0 10 20 30 40 50 60 70 80 30 100 110 120 130 140 150 160 170 180 130 200 210 220 230 24 Helalive Concentration 3 2
9. 78 20 2 vv 3 mL 40 C 50 nuL 25 10 000 pg mg MassHunter 1 x APCI ESI 20 000 pg mg
10. 100 pg mg V 15 15 20 20 25 20 25 25 20 15 25 15 COC LC MS MS LC MS MS COC 2 2 8 MRM 290 3 gt 168 3 3Coc hair003 d RT 5 197 Name Norcocaine Ralalye Abundance 49 5 51 52 53 54 55 56 57 Acgquisition Time min 2 100 pg mg COC BXE NO 25 10 000 pg mg
11. 2 48 5 D3 Benzoylecgoni Benzoylecgonine Benzoylecgonine Cocaine quant 2 1 22 23242526 272 829 3 313233343536373839 4 4142434445464748 Abundance vs Acquisition Time min MRM 326 3 gt 204 4 3Coc har002 d MRM 18 3 gt 82 2 3Coc haw002 d MRM 318 3 gt 196 4 3Coc har002 d MRM 293 3 gt 171 4 3Coc har002 d MRM 290 3 gt 136 3 3Coc haw002 d MRM 290 3 gt 168 3 3Coc haw002 d 4243444546474849 5 515253545556575859 10 D8 Cocaethylene Cocaethylene quant 1 D3 Norcocaine 6162636465666768639 Abundance vs Acquisition Time min 4 50 pg mg COC BZE CE NC LC MS MS
12. 6 GHB THC 9 THC MDMA MDA MDEA 7 o w
13. BZE GC MS 8 GC 4 LC MS MS APCI COC 2 5 6 COC BZE MRM 1 2 1 2 Maralikova Weinmann LC MS MS 3 Agilent Technologies
14. 2 MS Agilent 6410 Agilent G1947B APCI 400 C 5 L min 350 C 50 psig Vig 4500 V 4nA FWHM 01 2 5 amu 02 0 7 amu MRM 50 msec 2 120 V 1 M 1 20
15. LC MS MS e g Oe er 0 Christine Moore Cynthia Coulter and Katherine Crompton Immunalysis Corporation 829 Towne Center Drive Pomona CA 91767 USA Michael Zumwalt Agilent Technologies Inc 9780 S Meridian Blvd Englewood CO 80112 USA HPLC LC MS MS APCI 20
16. BZE d3 COC d3 NC d3 CE d8 Cerilliant Round Rock TX Clin II 691 0353T SPEWare San Pedro CA HPLC ACS 200 ng mL 20 C 10 mg 8 25 50 100 200 500 1 000 2 000 10 000 pg mg 1 000 pg mg 10 mg 1 5 mL
17. 2 7 Johansen Bhatia LC MS MS COC 8 2 MRM LC MS MS COC 1 Cocaine Cocaethylene CHz1N04 CaHzsN04 M H 304 1 M H 318 2 0 0 CH 0 0 cn HiC HaC 0 0 0 0 Norcocaine Benzoylecgonine CeHisN04 CeHisN04 M H 290 1 M H 290 1 0 0 Wh 0 0O 0 N oo CH 1 HO N
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