Agilent ZORBAX Eclipse Plus C18 カラムとAgilent HPLC による既存アミノ酸 analysis 法の改良 Manual
Contents
1. Agilent LC Z0RBAX Eclipse Plus C18 1 Rainer Schuster and Alex Apfel Hewlett Packard App Note Pub 5954 6257 1986 2 Rainer Schuster J Chromatogr 431 271 284 1988 3 Herbert Godel Petra Seitz and Martin Verhoef LC GC International 5 2 44 49 1992 4 John W Henderson Jr Robert D Ricker Brian A Bidlingmeyer and Cliff WWoodward Rapid Accurate Sensitive and Reproducible HPLC Analysis of Amino Acids Agilent Pub 5980 1193E 2000 5 Cliff Woodward John W Henderson Jr and Todd Wielgos High Speed Amino Acid Analysis AAA on Sub Iwo Micron Reversed phase RP Columns Agilent Pub 5989 6297EN 2007 6 Angelika Gratzfeld Huesgen Sensitive and Reliable Amino Acid Analysis in Protein Hydrolysates using the Agilent 1100 Series HPLC Aqilent Pub 5968 5658EN 1999 7 Jason Greene John W Henderson Jr John P Wikswo Rapid and Precise Determination of Cellular Amino Acid Flux R2. 5 5 nm 5 um 2 2 0 400 bar 3 0 mm id 4 6 mm id 57 1 250 mm 3 5 unm 1 8 unm 1 20 OPA 3 FMOC 20
3. 1 5 R 5 um 3 5 um 1 8 nm 4 6 x 50 mm 3 5 nm 2 1 x 150 mm 5 nm 4 6 x 150 mm 500 mAU 10 Pmax 186 bar Jr 599 8 al 25 5 75 175 a 300 Pmax 190 bar Rs 1 86 LL 0 A 15 10 125 15 175 mAU 00 Piwx 190 bar 1 80 all other pairs gt 2 0 8 Po 0 L bE i 25 5 15 10 175 _ 8 Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm 500 AU 1 Pmax 130 bar 85 Rs 1 98 8 L I 0 T mAU Rs 1 75 185 W LM AU Rs 1 61 30 Pmax 135 bar Rs a 8 II LA 10 15 20 25 30 9
4. RRHT Al Agilent ZORBAX Eclipse Plus C18 100 mm 1 8 um Im 1 Wen 20 2 6 x mm 1 8 nm 40 P N959964 902 9 a 30 8 10 mn 13 4 1 5 1 16 6 20 7 1 5 8 10 3 22 23 9 0 6 AA 0 T T T T T T 1 2 4 6 8 10 12 14 2 mAU 3 50 2 1 x 100 mm 1 8 nm 4 P N 959764 902 5 Rs 2 4 40 6 30 7 21 8 20 23 9 20 10 22 21 22 0 23 2 4 6 8 10 12 14 3 100 mm Agilent Z0RBAX Eclipse Plus C18 1 8 um 2 2 Rs 9
5. Agilent Z0RBAX Eclipse Plus C18 50 mm 1 8 um mAU 1 0 46x50mm 1 8nm Rs 1 9 2 P N 959941 902 ss 19 1 2 4 6 40 1 5 16 4 LL MI ht sa i 0 6 7 3 8 mAU 3 0x50mm 1 8 nm 1 6 7 16 9 80 P N 959941 302 0 1 1 3 6 Ee 4 2 4 5 mAU 2 1 x 50 mm 1 8 nm G 80 P N959741 902 15 0 0 MM 15 15 7 8 9 UL ul AI 2 Ek 21 nea 22 1 23 4 50 mm Agilent ZORBAX Eclipse Plus C18 1 8 um 5 um Agilent ZDRBAX Eclipse Plus C18 mAU 4 6 x 150 mm 5 nm a3 i 2 B7022 4104 a 0 ee 5 5 20 mAU 1 20 gt B8018 aa 1 04 oss 1 04 ais 1 02 1 a TT 0 Re 7 5 aas 1 20 12 5 15 17 5 20 B8032 ds4 1 04 2 oes 1 04 ai4is 1 02 0 T T T
6. Amino0uant Agilent ZORBAX Eclipse Plus C18 AA Amino0uant 2 1 x 200 mm 5 Im 2 1 x 150 mm 3 5 um Aminoduant Eclipse Plus C18 Aminouant Aminoduant 2 1 x 150 mm 3 5 im 5 Agilent 1200 SL HPLC Gin ASsn Trp nva Hyp Sar 2 Amino0uant Eclipse Plus C18 Z0RBAX
7. Eclipse Pus Amino0uant Amino0uant 9 100 50 mm 1 8 um 3 100 mm RRHT 2 3 5 um 1 100 mm 1 8 um 250 mm 5 um 400 bar Agilent 1200 SL
8. Agilent 0PA FMOC 1 6 2 LC 10 50 mm 1 8 um 1 5 R 10 250 mm 5 nm 40 R gt 2 3 5 um 150 mm 1 8 um R_ gt 2 100 mm 1 1 Agilent Z0RBAX Eclipse Plus C18 1 4 6 x 250 5 um 959990 902 2 3 0 x 250 5 um 3 46 x 150 3 5 um 959963 902 4 3 0 x 150 3 5 um
9. 21 0PA 338nm FMOC 265 nm 2 2 40 25 3 5 um 5nm 2 3 EE mAU 4 6 x 250 mm 5 um Agilent ZORBAX Eclipse Plus C18 250 mm 5 nm 1 25 P N 959990 902 2 3 20 12 Rs 2 4 4 5 3 1 2 7 9 6 34 11 13 14 19 10 96 8 1 7 5 1 8 5 21 22 23 9 M_ 0 1 T
10. G1376C WPALS 1 Agilent P N 5061 3339 2 5 LL 1 0 mL 3 5 uL 5 0 2 0PA Agilent P N 5061 3335 0 5 LL 6 4 0 uL 10 7 FMOC Agilent P N 5061 3337 0 4 uL 8 4 4 uL 10 9 32 uL 10 20 pL 8 11 12 0 1 13 OU ER Oi IN 4 5 pm 4 6 x 250 mm 3 0 x 250 mm P N 959990 902 P N B B 0 2 2 0 84 2 2 33 4 57 57 33 5 100 100 39 3 100 100 39 4 2 2 40 1 5 0 64 mL
11. 10 Agilent HPLC 0OPA FMOC Agilent Technologies 3 5 nm 5 1 8 nm 3 5 um 1 8 um 2 1 x 150 mm 3 5 um P N 79916AA 572 AminoOuant
12. HPLC A B B 1L A 2L 100 mL 100 mL A 0 4 mL H P0 4 C 0 1 N HCI 4 2 mL HCI 36 500 mL 4 C 0PA FM0C OPA OPA 7 10 OPA 100 pL
13. 1 1 4 6 x 150 mm 3 5 um 3 5 um 8 500 10 ChemStatiopn 9 G1311A 3 0 x 250 mm 4 14 500 4 6 x 150 mm 2 5
14. 50 mL 0 1 N HCI 10 nmol uL ISTD 5 mL 45 mL 4 C 2 5 100 pmol uL 250 pmol pL 1 nmol pL 3 2 UV 3 2 AA pmol pL 900 225 90 5 mL 18 nmol EAA 5 mL 5 mL 5 mL 15 mL 45 mL EAA 5 mL 20 mL 50 mL 5 mL EAA
15. Nva Sar Asn GIn Trp Hyp 1 g 5061 3339 5061 3337 5061 3335 5062 2479 Sigma Sigma Sigma Sigma 5181 1270 5182 0716 5182 0721 9301 1377 9301 1379 9301 1378 5061 3330 5061 3331 5061 3332 5061 3333 5061 3334 5062 2478 S 7907 S 9640 S 2002 79617 www adilent com chem jp Agilent Technologies Inc 2010 Printed in Japan January 12 2010 5990 4547JAJP Agilent Technologies
16. 10 FMOC FMOC 100 pL 10 1 5 mL 10 4 5 6 7 10 pmol L 1 nmol pL
17. http www chem agilent com cag Iccustom_1page asp amp rc at LCColumns 3 5 3 5 1 8 nm Agilent Z0RBAX Eclipse Plus C18 2 pL G1315 60024 1 3
18. N A G1312 87330 N A G1328 87600 G1328 87600 ALS 500 mm 500 mm ALS G1367A G1329A G1367 87101 G1313 87201 ALS G1313 87305 01090 87611 180 mm 105 mm G1316 A 3 LuL G1316 A 3 LuL 5021 1817 5021 1816 150 mm 105 mm 820950 936 4 821125 936 4 4 6 2 1 4 6 x 150 mm 1200 SL G1312B G1312 87330 5021 1823 400 mm G1367C G1367 87201 01090 87611 105 mm G1316 80003 1 6 pL 5021 1820 105 mm 820950 936 4 4 6 3 0 x 150 mm 1200 SL G1312B G1312 87330 5021 1823 400 mm G1367C G1367 87201 01090 87611 105 mm G1316 80003 1 6 pL 5021 1820 105 mm 821125 936 4 2 1 2 1 x 150 mm 1200 SL G1312B 5021 1823 400 mm G1367C 61367 87201 01090 87611 105 mm G1316 80003 1 6 pL 5021 1820 105 mm 821125 936 4 2 1 5 5 nm 3 5 mm 4 6 x 15
19. 5 REPRO0010 D REPRO0009 D REPRO0008 D REPRO0007 D REPRO0006 D REPRO0005 D REPRO0004 D REPRO0003 D REPRO0002 D 2 93 7 113 2 157 142 5 62 9 3 92 4 111 8 155 137 1 61 8 4 93 1 112 1 157 144 4 65 8 5 93 8 113 3 157 8 141 9 60 9 6 7 92 5 111 6 154 4 137 9 61 2 8 95 7 116 162 6 140 7 70 2 9 93 2 113 3 158 138 9 61 94 7 114 159 143 5 63 2 10 Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm 1 nmol pl 11 12 FMOC 2 6 5 3 RZ 10 1000 pmol pL 100 1000 pmol pL
20. 5 mL 5 mL 5 mL 10 nmol ISTD 5 mL 5 mL 5 mL EAA ISTD 10 mL 10 mL 10 mL 100 pL EAA_ISTD 100uL 100uL 100 1 nmol AA 900 pL 250 pmol AA 900 pL 100 pmol AA 900 pL EAA 500 pmol nL ISTD 1 mL 1 mL 1 mL AA 3 AA pmol pL 90 22 5 9 5 mL 1 8 nmol EAA 5 mL 5 mL 5 mL 15 mL 45 mL EAA 5 mL 20 mL 50 mL 5 mL EAA 5mL 5 mL 5 mL 1 nmol ISTD 5 mL 5 mL 5 mL EAA ISTD 10 mL 10 mL 10 mL 100 pL EAALISTD 100nL 100 pL 100 pL 100 pmol AA 900 pL 25 pmol AA 900 pL 10 pmol AA 900 pL EAA 50 pmol nL ISTD 1mL 1 mL 1 mL AA LC 4 x10 6 bar A 35 B 80 A B 20 LL ALS
21. 2 mAU 1 3 25 3 0 x 250 mm 5 nm 4 P N 1 2 3 5 20 6 7 15 8 10 9 20 5 21 0 22 23 10 25 30 1 250 mm Agilent Z0RBAX Eclipse Plus C18 5 nm mAU 46x150 mm 3 5 pm AgilentZ0RBAX Eclipse Plus C18 150 mm 3 5 um RR 1 P N959963 902 2 40 12 gt 2 3 9 20 ur 3 1 7 I UE 2 3 22 23 6 0 ll A 7 T T T IE a T T 25 5 75 10 125 15 17 5 8 mAU 9 E 3 0 x 150 mm 3 5 nm PE 3 P N959963 302 Rs 2 0 40 1 2 20 3 4 0 J 5 T T T a T T 6 2 5 5 75 10 15 175 mAU 8 7 8 40 2 1 x 150 mm 3 5 pm 9 P N 959763 902 Res 20 20 21 22 EE 23 175 2 150 mm Agilent Z0RBAX Eclipse Plus C18 3 5 nm
22. 5 17 P N 5061 3330 5061 3334 1 mL 100 pL 4 C EAA 59 45 mg 59 00 mg 65 77 mg 91 95 mg 25 mL 0 1 N HCL 18 nmol uL EAA 5 mL 45 mL 1 8 nmol uL ISTD 58 58 mg 50 mL 44 54 mg
23. 1200 Agilent Z0RBAX Eclipse Plus C18 3 0 x 250 mm 5 nm 10 9 RSD 5 7 mAU 80 2 1 x 150 mm 3 5 um 4 6 x 100 mm 1 8 um 2 3 100 250 1000 pmol pL 10 25 pmol uL
24. 3 5 um 4 6 x 150 mm 3 0 x 150 mm 2 1 x 150 mm P N 959963 902 P N 959963 302 P N 959763 902 B B B 0 2 2 2 0 5 2 2 2 20 57 57 57 20 1 100 100 100 23 5 100 100 100 23 6 2 2 2 25 1 5 0 64 0 42 mL 1 8 pm 100 mm 4 6 x 100 mm 2 1 x 100 mm P N 959963 902 P N 959763 902 B B 0 2 0 35 2 2 13 4 57 57 13 5 100 100 15 7 100 100 15 8 2 2 16 5 0 42 mL 1 8 nm 50 mm 4 6 x 50 mm 3 0 x 50 mm 2 1 x 50 mm P N 959941 902 P N 959941 302 P N 959741 902 B B B 0 2 2 2 0 2 2 2 2 7 67 57 57 57 7 77 100 100 100 8 3 100 100 100 8 4 2 2 2 9 2 0 0 85 0 42 mL G1329A ALS 1 Agilent P N 5061 3339 2 5 u 1 0 upL 3 5 pL 5 0 2 0PA Agilent P N 5061 3335 0 5 uL 4 0 pL
25. 4 DAD 1 8 um 1200 SL 3 5 6 7
26. 4 6 x 100 mm 1 8 nm 2 1 x 100 mm 1 8 nm LC 1200 SL 1200 SL G1312 B G1312 B G1312 87330 5021 1823 5021 1823 ALS 400 mm 400 mm ALS G1367C G1367C G1367 87201 G1367 87201 ALS 01090 87611 01090 87611 105 mm 105 mm G1316 80003 1 6 pL G1316 80003 1 6 pL PN 959964 902 959764 902 G1315C G1315C 2 unL G1315 60024 2 nuL G1315 60024 Agilent Z0RBAX Eclipse Plus C18 5 P N 820888 901 B 3 0 x 250 mm 5 nm LC
27. 10 FMOC Agilent P N 5061 3337 0 4 pL 8 4 4 pL 10 9 32 10 20 pL 8 11 12 0 1 13 ALS G1367C 2 x 56 G2258 44502 1 2 OPA 3 FMOC 4 P1 A 1 nn OL p 5 TCC 40 C 0 8 C 5 DAD A 338 nm 10 nm 390 nm 20 nm B 262
28. _ Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm 10 1000 pmol uL 120 y 0 0879x 0 2168 R 0 9999 Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm y 0 1029x 0 5381 R 0 9998 T uf T T 400 600 800 1000 1200 pmol pL Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm y 0 069x 0 8281 R 0 9995 1 xs 40 1 TT 1 TT 0 400 600 800 1000 1200 0 200 pmol uL Agilent ZORBAX Eclipse Plus C18 2 1 x 150 mm 3 5 nm 80 y 0 1193x 3 0495 2 R 0 9972 60 40 20 I ME 1 Wg I 0 400 600 800 1000 1200 0 200 pmol uL T T 400 600 800 1000 1200 pmol nL 11 Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 um 5 0 0 Agilent Z0RBAX Eclipse Plus C18 4 6 x 100 mm 1 8 nm 10 1000 pmol uL 100 y 0 067x 0 2867 Agilent Z0RBAX Eclipse Plu
29. 959963 302 5 2 1 x 150 3 5 nmt 959763 902 6 4 6 x 100 1 8 nm 959964 902 7 2 1 x 100 1 8 nm 959764 902 8 4 6 x 50 1 8 um 959941 902 9 3 0 x 50 1 8 um 959941 302 10 2 1x 50 1 8 um 959741 902 LC LC Agilent ZORBAX Eclipse Plus C18 Agilent 1100 Agilent 1100 400 bar Agilent 1200 600 bar Agilent 1200 10 LC 2 1 x 150 mm 3 5 um Amino0uant 2 1 x 150 mm Amino Quant A 10 mM Na HP0 10 mM Na
30. B 0 pH 8 2 5 mM NaN 1 970 mL 1 4 g NayHP0 3 8 g Na B 0 10H 0 32 mg NaN pH 8 4 pH 8 2 1 M pH 1 L NaN mL Agilent HPLC 40 2 4 64 1200 or 1200 SL 40 2 4 28 1200 or 1200 SL 25 2 42 1200 or 1200 SL 25 2 18 1200 or 1200 SL 25 2 12 1200 or 1200 SLt 16 2 4 28 1200SL 16 2 4 8 1200SL 9 1 7 23 1200SL 9 1 7 10 1200SL 9 1 7 5 1200SL DAD 2 42 AminoQuant 1200SL Amino0uant 0 45 pm Agilent P N 3150 0576 B 45 45 10 v v
31. nm 16 nm 324 nm 8 nm C 338 nm 10 nm 390 nm 20 nm 262 nm 16 nm 324 nm 8 nm C 20 21 A B A B RRHT 1 8 um gt 0 01 RR 3 5 um gt 0 03 5 um gt 0 03 3 5 um 5 nm 4 6 x 250 mm 3 0 x 250 mm LC 1100 1200 G1312A G1311A quat
32. y 0 0723x 0 9605 0 9998 y 0 0554x 0 2005 0 9999 GLY y 0 0971x 2 1402 0 9997 y 0 0762x 0 4390 1 0000 THR y 0 0996x 0 7722 1 0000 y 0 0742x 0 4565 0 9999 ARG y 0 1011x 1 4018 0 9998 y 0 0768x 0 1926 0 9999 ALA y 0 1037x 1 1823 0 9999 y 0 0771x 0 5060 0 9997 TYR y 0 0941x 1 0366 0 9999 y 0 0714x 0 3964 1 0000 CYS y 0 1489x 3 4631 0 9997 y 0 1201x 0 4196 0 9999 VAL y 0 1038x 1 1299 0 9999 y 0 0792x 0 4669 0 9998 MET y 0 1016x 1 1133 0 9999 y 0 0779x 0 1347 1 0000 PHE y 0 0954x 1 2188 0 9999 y 0 0728x 0 4522 1 0000 ILE y 0 0990x 1 0161 0 9999 y 0 0737x 0 5095 0 9999 LEU y 0 1009x 1 1797 0 9999 y 0 0765x 0 3618 0 9999 LYS y 0 1233x 6 2802 0 9993 y 0 1261x 4 6725 0 9986 PRO y 0 0686x 1 0919 0 9994 y 0 0635x 1 6958 0 9995 13 FMOC 30 3mm G1315 60024 4 6 x100 mm 1 8 nm mAU 20 10 3 0 L T T T T T T eR 2 4 6 8
33. 0 mm 959963 902 N A N A G1315C 2 pL G1315 60024 1 8 pm 50 mm 4 6 x 50 mm 1 8 nm 1200 SL G1312 B G1312 87330 5021 1823 400 mm G1367C G1367 87201 01090 87611 105 mm G1316 80003 1 6 pL 959941 902 G1315C 2nL G1315 60024 3 0 x 150 mm 959963 302 N A N A G1315C 2 pL G1315 60024 3 0 x 50 mm 1 8 nm 1200 SL G1312 B 5021 1823 400 mm G1367C G1367 87201 01090 87611 105 mm G1316 80003 1 6 pL 959941 302 G1315C 2 pL G1315 60024 2 1 x 150 mm 959763 902 N A N A G1315C 2 pL G1315 60024 2 1 x 50 mm 1 8 nm 1200 SL G1312 B 5021 1823 400 mm G1367C G1367 87201 01090 87611 105 mm G1316 80003 1 6 pL 959741 902 G1315C 4 6 x 250 mm 3 0 x 250 mm 959990 902 5065 9931 5065 9931 200 mm 200 mm ZDV 5022 2184 5022 2185 G1315B G1315D 2 nlL G1315 60024 2 uL G1315 60024 1 8 nm 100 mm
34. 10 12 30 6 mm G1315 60011 3 46x100mm 1 8 pm 20 T 6 6 6 T 0 0 B T T T 2 4 6 8 10 12 13 100 mm Agilent Z0RBAX Eclipse Plus C18 RRHT 3 10 Agilent Z0RBAX Eclipse Plus C18 2 1 x 150 mm 3 5 um Amino0uant 1 LC LC 400 bar LC Agilent 1100 600 bar LC Agilent 1200 SL LC 2
35. 9999 ARG y 0 1001x 0 5964 0 9997 y 0 0769x 0 1386 0 9999 ALA y 0 1029x 0 5381 0 9998 y 0 0773x 0 3254 0 9998 TYR y 0 0934x 0 4675 0 9998 y 0 0716x 0 1888 1 0000 CYS y 0 1472x 2 0912 0 9994 y 0 1202x 0 5441 0 9998 VAL y 0 1029x 0 3804 0 9998 y 0 0794x 0 2804 0 9999 MET y 0 1008x 0 4284 0 9998 y 0 0780x 0 0927 1 0000 PHE y 0 0945x 0 5314 0 9998 y 0 0730x 0 2714 0 9999 ILE y 0 0982x 0 3882 0 9998 y 0 0739x 0 3198 0 9999 LEU y 0 1001x 0 5408 0 9998 y 0 0767x 0 2263 0 9999 LYS y 0 1193x 3 0495 0 9972 y 0 1229x 2 1018 0 9976 PRO y 0 0690x 0 8281 0 9995 y 0 0641x 1 1849 0 9992 5 LC LC LC 2 1 mm x 150 mm 3 5 pm 4 6 mm x 100 mm 1 8 nm R2 R lt ASP y 0 0958x 0 7365 0 9999 y 0 0710x 2 2162 0 9982 GLU y 0 0884x 0 6010 1 0000 y 0 0668x 0 4858 0 9999 SER y 0 0995x 1 2270 0 9999 y 0 0745x 0 2204 1 0000 HIS
36. Agilent ZORBAX Eclipse Plus C18 Agilent HPLC e John W Henderson Jr HPLC HPLC HP 1090 HPLC Anne Brook EN 9 0PA FMOC Agilent Technologies Inc Agilent 1100 HPLC Agilent 1200 SL 2850 Centerville Rd Agilent Z0RBAX Eclipse Plus C18 Wilmington DE 19808 1 5 USA 2 3
37. T T T 5 125 15 17 5 20 5 Agilent Z0RBAX Eclipse Plus C18 5 um 3 3 5 nm ains 1 02 as 1 21 ds4 1 06 mAU 2 1 x 150 mm 3 5 pm ags 1 04 4 B7044 3 Eh as 1 02 4 0 AU I T T 1 T I I T 2 5 5 75 10 12 5 15 17 5 20 AU B7107 gl m 4 ds4 1 06 9es 1 04 qa 1 02 A I T T T T I T 2 5 5 7 5 10 12 5 15 17 5 20 as 1 20 mA B8022 ds4 1 06 0 T T T T T T T T 2 5 5 7 5 10 12 5 15 17 5 20 6 Agilent Z0RBAX Eclipse Plus C18 3 5 um 3 1 8 nm Agilent Z0RBAX Eclipse Plus C18 qa 1 21 mAU 46 x 50 mm 1 8 nm ds4 1 05 6 4 ags 1 04 B7056 aiais 1 02 4 3 5 6 4 0 A ES 1 HE 1 2 3 4 5 6 7 mAU qa 1 21 se B7125 7 ags 1 04 413 1 02 4 I 0 4 NE A 1 2 3 4 5 6 7 mAU as 1 21 6 800A asa 1 04 ags 1 04 ata 1 02 4 E 0 1 A 6 1 1 2 3 4 5 6 7 7 Agilent Z0RBAX Eclipse Plus C18 1 8 um
38. ates Using HPLC with Automated Derivatization with Absorbance Detection Agilent Pub 5990 3283EN 2009 7 0 4 M pH 10 2 100 mL FMOC ACN 2 5 mg mL 10 x 1 mL OPA 0 4M 10mg mL 3 6 x 1 mL DTDPA 5 g Na HPO Na B40 10H 0 NaN H P0 100 uL 2 mL 100 PTFE 100 6 mL 6 mm 100 16 mm 100 PTFE 16 mm 100 0 1 M HCI 10 x 1 mL 1 nmol mL 250 pmol mL 100 pmol mL 25 pmol ml 10 pmol mL
39. s C18 4 6 x 100 mm 1 8 nm Agilent Z0RBAX Eclipse Plus C18 4 6 x 100 mm 1 8 nm y 0 0773x 0 3254 R 0 9998 R 0 9999 80 60 40 J 20 0 T 400 600 800 1000 1200 0 200 pmol pL y 0 1229x 2 1018 T i T T 400 600 800 1000 1200 pmol nL Agilent Z0RBAX Eclipse Plus C18 4 6 x 100 mm 1 8 nm y 0 0641x 1 1849 R 0 9992 R 0 9976 S 4 20 T T 1 T 0 1 400 600 800 1000 1200 0 200 pmol uL 400 600 800 1000 1200 pmol pL 12 Agilent Z0RBAX Eclipse Plus C18 4 6 x 100 mm 1 8 nm 5 6 5 3 RZ 5 RZ 10 1000 pmol pL 3 RZ 100 1000 pmol pL 2 1 mm x 150 mm 3 5 pm 4 6 mm x 100 mm 1 8 nm R2 R lt ASP y 0 0952x 0 2994 0 9999 y 0 0723x 1 1861 0 9976 GLU y 0 0879x 0 2168 0 9999 y 0 0670x 0 2867 0 9999 SER y 0 0983x 0 2968 0 9996 y 0 0746x 0 1128 1 0000 HIS y 0 0716x 0 4109 0 9997 y 0 0555x 0 1748 1 0000 GLY y 0 0956x 0 8918 0 9993 y 0 0758x 0 1374 0 9999 THR y 0 0990x 0 3423 0 9999 y 0 0744x 0 2991 0
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