Agilent Easy Calibration of OligoPore Columns (DMF) Manual
Contents
1. Easy Calibration of Agilent OligoPore Columns DMF Technical Overview Introduction The calibration of OligoPore columns in DMF with 0 1 w v LiBr is straightforward using Agilent polyethylene glycol PEG narrow standards at 80 C with the integrated Agilent PL GPC 220 GPC SEC system The PEG standards used are available as a kit containing ten standards in the range 106 20 000 g mol PEG 10 OligoPore columns make use of an innovative new medium that exhibits significantly increased pore volumes compared to conventional low pore size columns for gel permeation chromatography resulting in higher resolution in the oligomeric region OligoPore columns have been specifically designed for the analysis of oligomeric samples and the fingerprinting of oligomeric distributions in organic solvents OligoPore columns can be used with a wide variety of organic solvents ranging from solvents of low polarity such as dichloromethane to highly polar organic solvents such as dimethyl formamide DMF The relatively high vicosity of DMF compared to other solvents such as tetrahydrofuran makes high temperature SEC appealing Increasing the column temperature to 80 C not only reduces the viscosity of DMF therefore reducing back pressure but also leads to increased efficiency and hence resolution In addition to reduce interactions between the column packing material and the samples a small quantity of a salt such as LiBr is added Agg2. Agilent Technologies Figure 1 shows overlaid chromatograms of from top to bottom PEG 4120 1470 1080 620 400 194 and 106 In each case the negative peak eluting after the PEG is due to compositional differences between the sample solvent and the eluent Figure 2 shows the resulting calibration curve for the OligoPore columns The curve indicates that the two OligoPore columns have a linear response to Increasing molecular weight up to the exclusion limit of the column Conditions Columns 2 x OligoPore 300 x 7 5 mm p n PL1113 6520 Samples Polyethylene glycol narrow standards 0 1 w v Eluent DMF 0 1 LiBr Flow Rate 1 0 mL min Injection Volume 100 uL Detection RI These data represent typical results For further information contact your local Agilent Sales Office 5 min 20 Figure 1 Overlaid chromatograms of PEG 4120 1470 1080 620 400 194 and 106 top to bottom 4 5 log M 10 min 18 Figure 2 Calibration curve for the OligoPore columns www agilent com chem This information is subject to change without notice Agilent Technologies Inc 2010 Published in UK September 20 2010 SI 01732 Agg Agilent Technologies
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Agilent Easy Calibration of OligoPore Columns (DMF) Manual