Agilent Polystyrene Sulfonate Analysis by Aqueous SEC Manual
Contents
1. PL aquagel OH MIXED H columns These data represent typical results For further 33 information contact your local Agilent Sales Office www agilent com chem This information is subject to change without notice Agilent Technologies Inc 2010 Published in UK September 20 2010 S1 01660 Agilent Technologies2. Polystyrene Sulfonate Analysis by Aqueous SEC Agilent PL aquagel OH MIXED H 8 um Columns Technical Overview Introduction The PL aquagel OH packing exhibits excellent stability and so eluent modification is possible with no loss of the high column efficiency gt 35 000 plates meter This ability permits the investigation of hydrophobic compounds such as polystyrene sulfonate by aqueous size exclusion chromatography To counteract ionic interactions between polystyrene sulfonate standards and the column packing the eluent is modified by the addition of salt Due to the hydrophobic nature of these samples further modification of the eluent is done by the addition of a weak organic solvent methanol EE Agilent Technologies Conditions Columns 3x PL aquagel OH MIXED H 8 um 300 x 7 5 mm p n PL1149 6800 Eluent 0 2M NaNO 0 01M NaH PO at pH 7 30 methanol Flow Rate 1 mL min Detector RI Figure 1 is a typical raw data chromatogram for a mixture of polystyrene sulfonate standards showing how the PL aquagel OH MIXED H 8 um column offers excellent resolution over a broad molecular weight range This ability to deliver high resolution over a very wide range of molecular weights simplifies column selection and provides a versatile analytical system Peak Identification 1 400 000 2 35 000 3 4 600 10 min Figure 1 Excellent separation of a broad range of polystyrene sulfonate standards using
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Agilent Polystyrene Sulfonate Analysis by Aqueous SEC Manual