Agilent 500 Ion Trap LC/MS Atmospheric Pressure Ionization Interface Manual
Contents
1. 2 pE ae ERNIS HARS IRT ee 250 500 1000 of consecutive injections Figure 4 Reproducibility of 1000 injections shows no contamination of the API Benefits The unique design on the Agilent 500 lon Trap LC MS Atmospheric Pressure lonization Interface reduces interface contamination and delivers robust consistent system sensitivity www agilent com chem Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material Information descriptions and specifications in this publication are subject to change without notice Agilent Technologies Inc 2011 Printed in the USA January 16 2011 SI 0241 Ee Agilent Technologies2. ion guide has no line of sight between the axis of the capillary and the ion guide thus preventing droplets from the spray assembly from entering the mass analyzer and causing noise The heated counter flow drying gas facilitates evaporation of droplets to form sample ions while also displacing uncharged contamination from the sample matrix from entering the vacuum chamber and ion optics Heated counter flow drying gas Capillary 6 off axis from the ion guide a eS Needle off axis from Droplets si the capillary Drain aligned with capillary Figure 3 Tilted chamber design reduces contamination 2 Experimental Conditions and Results The hexapole ion guide robustness when coupled with an ion trap detector is demonstrated with the results in Figure 4 Test samples of 1 ppm 4 hydroxy tolbuta min in 10 urine in 50 50 methanol water were injected multiple times into the 500 lon Trap system No sample preparation was conducted and direct injections were performed One thousand consecutive injections were made with the results shown in Figure 4 After 1000 injections the response factor remained constant with no indication of contamination of the API interface or long term changes in system sensitivity Area response 500 lon Trap Reproducibility Hexapole lon Guide Mil Counts 1000 injections RSD 10 0 ag ee ry ne rh s 2 ing BO UNE Shy ears wn r e Series1 Wie s Sa ayit bay
3. Agilent 500 lon Trap LC MS Atmospheric Pressure lonization Interface Technical Overview Introduction Routine analysis of large numbers of samples in a dirty matrix presents a severe challenge for an LC interface The ionization chamber and atmospheric pressure ion ization API interface shown in Figure 1 is designed to meet just such a challenge lons formed at atmospheric pressure in the electrospray ES assembly are directed from atmospheric pressure into the low pressure region of the MS vacuum system via the interface capillary and are subsequently transported by the hexapole ion guide into the mass analyzer Sample spray assembly Hexapole ion guide Capillary Spray chamber Figure 1 The Agilent API Interface EE Agilent Technologies Product Description The electrospray assembly Figure 2 allows adjustment of the spray tip in two dimensions to optimally position the spray relative to the interface capillary Adjustment of the extension of the liquid capillary from the nebulizing sheath allows the optimal formation of electrospray droplets depending on the liquid flow rate and solvent composition Figure 2 Adjustment of the inner liquid capillary needle to maximize nebulization effeciency The tilted chamber Figure 3 ensures that the excess liquid from the spray will be directed into the drain port at the bottom of the chamber and away from the inter face capillary to reduce contamination The tilted
Download Pdf Manuals
Related Search
Agilent 500 Ion Trap LC/MS Atmospheric Pressure Ionization Interface Manual