Agilent ELS UV Detection of Succinic Acid Manual
Contents
1. Figure 3 20 pg succinic acid using Agilent ELSD The Agilent ELS detector gives a much greater response than the UV type as succinic acid has a very poor UV chromophore It is possible to detect succinic acid down to an on column load of 2 ug with a 1 mg mL sample Figure 4 Ni 0 min 10 Figure 4 Limits of detection for succinic acid with the Agilent ELSD These data represent typical results For further information contact your local Agilent Sales Office www agilent com chem This information is subject to change without notice Agilent Technologies Inc 2011 Published in UK July 13 2011 5990 8439EN 7 Agilent Technologies2. ELS and UV Detection of Succinic Acid Technical Overview Introduction The Agilent Evaporative Light Scattering Detector delivers clean baselines no drift no solvent peaks and much greater response than a UV detector when analyzing succinic acid Succinic acid is an organic acid widely used in industry as an additive in products such as paints clothing and many food products It can be made from fermentation of glucose and used as a 4 carbon block for polymers EE Agilent Technologies This dicarboxylic acid does not have a good UV chromophore and therefore the method of detection if the solute is not to be denatured is RI However the separation of succinic acid from other substances that require gradient elution such as proteins rule out the use of RI detection This meant that UV detection at low wavelengths was the preferred method of detection Figure 2 OH SS o OH Figure 1 Structure of succinic acid Conditions Sample 1 mg mL Column PLRP S 300A 8 um 4 6 x 150 mm p n PL1512 3801 Eluent A 1 ACN 0 1 TFA 99 Water Eluent B 100 ACN 0 1 TFA Gradient 5 80 B in 20 min Flow Rate 1 0 mL min Detection UV 215 nm Agilent ELSD 0 min Figure 2 20 pg succinic acid using UV detection The use of the Agilent ELSD overcomes these difficulties With the Agilent ELSD the baseline is stable without any drift and no solvent peak is detected Figure 3 10 Pe 0 min 10
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Agilent ELS UV Detection of Succinic Acid Manual