Agilent Using the Agilent Instrument Control Framework to control the Agilent 1220 Infinity LC system through Waters Empower software Manual
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1. 0 a o o e e Author A G Huesgen Agilent Technologies Inc Waldbronn Germany Using the Agilent Instrument Control Framework to control the Agilent 1220 Infinity LC system through Waters Empower software Instrument set up and performance Technical Overview 192 168 0 5 Abstract The Agilent Instrument Control Framework ICF enables other providers of LC data acquisition and processing software to simplify the development of the control of Agilent LC instrumentation In this Technical Overview we demonstrate how Agilent ICF facilitates enhanced control of the Agilent 1220 Infinity LC system through Waters Empower chromatography data software versions 2 and 3 The combination of Agilent ICF and Waters Empower software provides easy access to advanced features of the 1220 Infinity LC system such as overlapped injections Aig Agilent Technologies Introduction The Agilent Instrument Control Framework ICF is a software com ponent that makes it easier and faster for software providers to implement control of Agilent LC equipment in their chromatographic data systems or workstations Based on new standard instrument drivers from Agilent ICF eliminates much of the delay and effort of using low level instrument control codes and the need of software devel opers to write their own native drivers In this Technical Overview we demonstrate e What prerequisites have2. and ICF e Only Agilent 1220 Infinity LC sys tems containing an autosampler are supported Instruments with a manual injector are not supported by Empower 2 or 3 All Agilent LC modules must have firmware version A 06 32 or B 06 32 or B 06 41 or higher All Agilent LC modules must have RC Net drivers e Agilent Infinity LC ICS 1 0 0 Empower 2 feature release 3 or higher or Empower 3 software e Windows XP with service pack 3 or higher Results and discussion Agilent ICF facilitates access to advanced features of Agilent LC instru ments that were previously not sup ported by earlier revisions of Empower with drivers provided by Waters Now all features are supported and avail able though the new On line screen which has been added to the familiar Empower screen see Figure 1 A right click on one of the module fields gives access to all control method and other advanced features of the module For supported modules and functions refer to the Appendix Configuring the Agilent 1220 Infinity LC system 1 Set up the DHCP of the Empower node 2 Set all DIP switches of the Agilent module to 0 This module is con nected to the Empower node through LAN 3 The LC receives an IP address from the DHCP server 4 To connect the Agilent instrument configure the DHCP server through Properties of the Empower Nodes 5 Use Edit to set the instrument type and a unique name 6 Click File and New ch
3. aptured while a sequence was running Creating a sequence A sequence is created by filling the sample set table with name of sample position of vials number of injection per vial method set to be used and so forth Performance of the Agilent LC systems using Waters Empower data processing tools To demonstrate that the Agilent 1220 Infinity LC system fulfills the expected performance the following tests were done using the RRLC Checkout sample e Precision of retention time e Precision of areas Precision of retention time and areas Precision of retention times and areas for a 1 uL injection are combined in Table 1 Data were evaluated using the Empower Component summary report The precision for the retention times for six consecutive runs is less than 0 24 RSD for the area the precision is less than 0 9 RSD RSD Area 1 pL injection Peak name RSD RT volume acetanilide 0 236 0 229 acetophenone 0 149 0 308 propiophenone 0 066 0 392 butyrophenone 0 034 0 518 benzophenone 0 041 0 334 valerophenone 0 051 0 459 hexanophenone 0 050 0 510 heptanophenone 0 039 0 605 octanophenone 0 038 0 838 Table 1 Precision of retention times and areas for six consecutive runs Conclusion The Agilent Instrument Control Framework ICF is a software com ponent that makes it easier and faster for software providers to implement control of Agilent liquid chromatogra phy systems in their chromatographi
4. c data systems or workstations In our application example ICF was used to control the Agilent 1220 Infinity LC system in combination with Waters Empower software The instrument was configured in Empower and data were acquired and processed The combination of ICF and Empower software facilitates access to all avail able Agilent instrument features such as overlapped injection The Agilent Instrument Status screen is used to set up On Line methods to switch the system on or off to equilibrate columns to view the status of single module and to access special features using the Control function available for each Agilent LC module As expected the Agilent 1220 Infinity LC system shows the same excellent performance for data acquired and processed using Empower and ICF References 1 The Agilent Technologies Instrument Control Framework Technical Overview Publication number 5990 6504EN November 2010 2 The Agilent Technologies Instrument Control Framework Short overview Publication number 5990 5756EN June 2010 3 Agilent Infinity LC Instrument Component software Version 1 0 for Empower software Waters Installation note Publication number 716003453 Rev A August 2011 4 Using the Agilent Instrument Control Framework to control the Agilent 1260 Infinity LC through Waters Empower software Instrument set up and performance Agilent Publication Publication number 5990 9092EN Nov
5. ember 2011 5 Using the Agilent Instrument Control Framework to control the Agilent 1290 Infinity LC through Waters Empower software Instrument set up and performance Agilent Publication Publication number 5990 9093EN November 2011 Appendix Supported Not supported G4286B Isocratic Manual Injector X G4288B C Gradient Manual Injector X G4290B C Gradient Autosampler X Table 2 Supported and not supported instruments www agilent com chem icf Agilent Technologies Inc 2012 Published in USA January 1 2012 Publication Number 5990 9399EN cgp Agilent Technologies
6. romatographic system to make the new LC system accessible for data acquisition After this last configuration step the Agilent 1220 Infinity LC system is On Line and ready for use These configuration steps have to be followed whenever a new module is added or removed Previously the old configuration has to be deleted from the DHCP server configuration Then the Empower software has to be shut down and the LAN connection to the module has to be switched off and on again When the LAN connection has been restored the new configuration procedure can be started Creating an instrument method and a method set Having configured the instrument the instrument method and the method set can be created in the Empower Run sample screen The Instrument method is set up through the Edit method All parameters that are available in the Agilent ChemStation are now typically accessible in Empower The created instrument method is saved and used to set up a method set The method set can then be used to create sequences i ane E E le W fea wo re 10 00 a Sarge Sat Tira Piarsaring O24007 Teld Sarde Tre Parnaring 024007 NewSeneleSet Tree 0100 Sample Set 2160 0092 otos 0166 00a omo Figure 1 Agilent Instrument Status screen under Empower software and ICF c
7. to be fulfilled to ensure seamless interac tion between Agilent 1220 Infinity LC systems Waters Empower software and Agilent ICF e How instruments are configured and methods are created for the Agilent 1220 Infinity LC system using Waters Empower software in combination with Agilent ICF That the performance of the Agilent 1220 Infinity LC system fulfills expectations in combination with Waters Empower data acquisition and processing tools Experimental An Agilent 1220 Infinity LC system with the following configuration was used for the precision measurement e Gradient pump e Autosampler e Column oven e Variable wavelength detector e Agilent ZORBAX RRHT Eclipse Plus C 18 column packed with 1 8 um particles Chromatographic conditions for precision measurement RRLC Checkout sample p n 5188 6529 acetanilide acetophenone propiophenone butyrophenone benzophenone valerophenone hexanophenone heptanophenone octanophenone Compounds Column Agilent ZORBAX RRHT Eclipse Plus C 18 4 6 x 100 mm 1 8 um Mobile phases A Water B Acetonitrile Gradient 20 to 80 B in 8 min at 9 min 80 B at 9 01 min 20 B Flow rate 1 2 mL min Stop time 12 min Column temperature 30 C Injection volume Tul VWD 245 10 Hz Software Empower 2 Build 2154 Installed Service Packs A D Installed feature releases 1 4 with ICF version A 01 02 SP1 Prerequisites for the combination of Empower
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Agilent Using the Agilent Instrument Control Framework to control the Agilent 1220 Infinity LC syste