Agilent Cell Solutions Series II Kit Manual
Contents
1. 3 detection live dead cell dyes and much more Adapt or develop your own protocols general application tools in the software allow you to adapt the system to your assay requirements or use predefined flow cytometric assays for easy startup Minimal sample consumption work with 10 ul cell suspension 20 000 down to 2 500 cells enables flow cytometry analysis of primary and other precious cells Automation simply load one to six samples onto a chip and start run On chip staining procedure speeds up workflow Analyze a large variety of cell samples the system works equally well on most kinds of eukaryotic cells Conveniently archived and stored digital data easily share data with others and export it for publication or presentation Single platform upgrade your 2100 bioanalyzer with the flow cytometry set G2948CA for the automated analysis of cells nucleic acids and proteins for B and C series instruments only Requires 2100 bioanalyzer not available for 2100 e bioanalyzer whee Agilent Technologies Solutions for a wide range of cell applications Transfection optimization Measure expression of green fluorescence protein GFP as reporter gene Transfection efficiency of Cy5 labeled siRNA to optimize delivery Counterstain with live cell dye in second reference color Measure cellular protein expression Apply antibody staining with direct labeled or secondary antibodies Expr2. qated 43 8 www agilent com chem contactus U S and Canada 1 800 227 9770 agilent_inquiries agilent com Europe info_agilent agilent com Asia Pacific adinquiry_aplsca agilent com GFP transfection experiment showing the overlay of mock transfected cells and GFP expressing cells Live cell population is defined by adjusting CBNF marker right his togram Result of 63 5 GFP positve cells within live cells is obtained by gating and adjustment of GFP marker left histogram Useful for monitoring the success of a wide range of transfection experiments e g pro tein expression or gene silencing using Cy5 labeled siRNA Cells can be differentiated depending on the expression of a protein marker The high sensitivity laser based red channel is used to detect and quantify signals from an anti body stain like APC allophycocyanin or Cy5 A counterstain with the live cell stain calcein allows to gate on living cells Annexin V binds to phosphatidylserine PS a membrane lipid that is kept to the inner leaflet of the cell membrane of intact cells Exposure of PS to the outer leaflet is an early indicator of apoptotic processes Annexin V binding is made detectable by Cy5 staining of the annexin V via biotin streptavidin interaction Calcein staining of the cells is used as a live control to distin guish living and apoptotic cells from dead cells Learn more www agilent com chem labonachip Buy online ww
3. Whether you are doing transfection experiments for protein expression studying apoptosis in cell cultures or looking to optimize your gene silenc ing experiments the Agilent cell kit makes it easy to measure fluores cence parameters from indiviual cells Specifications Automated analysis of up to 6 cell samples in less than 30 minutes Detection limit of 5 000 MESF high sensitivity red channel excitation 630 nm emission 680 nm FL4 and 2 000 000 MESF blue channel excitation 470 nm emission 525 nm FL1 With a recommended cell density of 2 0 x 10 mL usually 1000 cells are detected per sample Minimum cell throughput 2 5 cells sec Cell Kit Reorder number 5067 1519 Includes 25 Chips Chip priming station Focusing dye Cell buffer for density and viscosity 2 Cells buffer for handling few cells Cell Checkout Kit Reorder number 5067 1520 Includes 7 Checkout chips Chip priming station Focusing dye Cell buffer Red and blue checkout beads Advantages of the lab on a chip approach Cell Solutions Series Il kits On chip flow cytometry an easy way e to acquire cell based fluorescence parameters Easy to use system Short setup time and easy straightforward data analysis software makes flow cytometry applicable for everyone Analyze a broad range of cell parameters endogenous fluorophores such as GFP antibody staining or apoptosis detection by Annexin V binding as well as Caspase
4. ession of cell surface proteins Expression of intracellular proteins Apoptosis assays Measure apoptosis by Annexin V binding of live cells Apoptotic cells can also be detected by intracellular anti body staining of active Caspase 3 Detect DNA laddering DNA assay Kits for the Agilent 2100 bioanalyzer are available for the analysis of DNA RNA proteins and cells No of Events GFP lipofection6 GFP lipofection6 No of Events CBNF Mock transf Ctrl Reake 1 10 Total events 1398 soy ooy 1 1 suw ooy aya 1P 1P 10 10 Blue Huesen total 59 6 Total events 1160 20 expression souy Loru uiy nii Linu 1 1r 1 1 1 Blue Flucmscence total 32 1 10 expression Loop siy 10 1P 1P Blue Huese total 16 3 Total events 1069 5 expression o Blue Flucescence total 8 0 control 24h incubation ow cenacance TA Total events 170 Calcein Oo P E u M F Sa n gated 1 2 Total events 171 Annexin CY5 Calcein No of Carne sasra E 2 P Fhceouconce woo A gated 45 1 Total events 175 cenacence Annexin CY5 Calcein A e a P La oe Prceesconce Frcees gated 42 4 Total events 1736 Find an Agilent customer center in your country n orocorco nd n w cae ie ew w gated 40 6 Annexin CY5 Fherna gated 44 1 Annexin CYS Prenvacerce Z
5. w agilent com chem store Copyright 2004 2007 Agilent Technologies Published October 1 2007 Publication Number 5989 0231EN Agilent Technologies
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