Agilent 1260 Infinity Micro-scale Fraction Collector/Spotter handbook
Contents
1. Agilent 1260 Infinity Micro scale Fraction Collector Spotter Features Specifications and Ordering Details 1260 Infinity Micro scale Fraction Collector Spotter The Agilent 1260 Infinity Micro scale Fraction Collector Spotter is a multifunctional tool for precise collection or deposition of minute amounts of separated compounds at lowest carryover A range of easy to exchange capillary kits facilitate operation at flow rates from 0 1 pL min to 100 pL min The combination of different collection modes with special capillary coatings guarantees reliable deposition of smallest droplets without cross contamination This module is ideal for collecting microgram amounts of compounds for further MS analysis or for offline 2 dimensional HPLC It is also perfectly suited for HPLC MALDI spotting even with automated online matrix addition The optional 1260 Infinity Thermostat provides Peltier cooling for your thermally labile chemical or biochemical samples Features e Seamless integration of the 1260 Infinity Micro scale Fraction Collector Spotter within a system minimizes internal volumes for highest separation performance and sensitivity e Collection in different well plates 96 384 standard or conical in Eppendorf tubes 0 5 1 5 or 2 mL or spotting on MALDI targets from all major vendors gives you infinite flexibility for your fractions e Liquid contact control enables for reproducible and robust collection of microliter and nanoliter fra2. ctions e Peltier cooling prevents sample evaporation or thermal decomposition e Thermostatting of MALDI targets and online matrix addition facilitates superior crys tallization between matrix and sample analytes e Fast droplet deposition with minimum 3 seconds per fraction e Hydrophobic capillary coatings minimize cross contamination e Capillary kits enable highest performance with minimum peak dispersion RE Agilent Technologies Specifications Agilent 1260 Infinity LC Micro Collection Spotting System Agilent 1260 Infinity LC Micro Collection Spotting System specifications Performance specs System delay volume typical System control GLP features Baseline noise 3 x 10 5 AU at 254 nm at flow rates lt 100 pL min 5 uL from EFC to column head for flow rates up to 20 pL min default setup 14 uL from EFC to column head for flow rates up to 100 pl min default setup Through local computer software LAN or local handheld control module Early maintenance feedback EMF tracks lamp burn time usage number of injections with limits and feedback messages Agilent 1260 Infinity Micro Collector Spotter Delay volume Fraction collection containers MALDI carriers Fraction capacity Maximum collector capacity Typical volumes for spotting Spotting frequency Matrix addition Maximum system flow Minimum fraction volumes Cooling Trigger modes Trigger source Environment Typically 0 25 pL with 25 um ca
3. pillary 1 uL with 50 um capillary 4 pL with 100 um capillary 384 and 96 well plate format standard and conical shape eppendorf tubes 0 5 1 5 2 0 ml For Agilent Applied Biosystems Bruker and Micromass targets 4 well plates 4 MALDI plates or 4 x 27 Eppendorf tubes 2 micro fraction collector spotter in parallel with 2PS 6PT micro valve 100 5000 nL lt 0 33 Hz With syringe pump 100 pL min higher flow rates requires system modifications Typically 2 pL depending on fraction collection container With G1330B Peltier Thermostat Time and peak based threshold up downslope and time table combination of different modes Agilent 1260 Series MWW and DAD detectors and third party detectors requires UIB 4 55 C constant temperature 95 humidity non condensing Specifications Agilent 1260 Infinity Modules e Agilent 1260 Infinity Nanoflow pump e Agilent 1260 Infinity Capillary pump e Agilent 1260 Infinity Micro Vaccum Degasser e Agilent 1260 Infinity Micro Well plate Autosampler e Agilent 1260 Infinity High Performance Autosampler e Agilent 1260 Infinity Thermostatted Column Compartment e Agilent 1260 Infinity Diode array Detector e Agilent 1260 Infinity Multiple Wavelength Detector Ordering Details Agilent 1260 Micro Collector Spotter Description Product Number Agilent 1260 Infinity Micro scale Fraction Collector Spotter G1364D Includes well plate tray and 25 50 and 100 um capillaries For MALDI spot
4. ting Online Matrix Kit G1364 68706 Syringe Pump 3162 0178 Syringe Adapter 9301 1291 Maldi Plate Carrier Applied Biosystems 5022 6542 Calibration plate 192 spots 5023 0209 Calibration plate 10x10 20x20 5023 0209 Maldi Plate Carrier ABI Opti TOF 5023 0238 Maldi Plate Carrier Agilent Target 5022 6543 Calibration Plate 5023 0214 Maldi Plate Carrier Bruker Plate 5022 6541 Calibration Plate 5023 0208 Maldi Plate Carrier Bruker PAC 5022 6546 Maldi Plate Carrier Micromass Target 5022 6544 Calibration Plate 5023 0215 Target Plate for AP MALDI LC MS G1972 60025 Agilent 1200 Series Thermostat gt G1330B For ALS spotter FC with Peltier temperature control MALDI Spotting tips PTFE 10 pk G1364 81701 Currently the Agilent 1260 Infinity Collector Spotter supports the following targets preconfigured in ChemStation Micromass 96 Micromass Sample 96 Micromass Lock 84 Micromass Sample 84 Micromass Lock 84 Micromass Calib Agilent 96 Agilent for AP MALDI G1972 60025 Bruker 384 AnchorChip Sample 384 AnchorChip Lock 1536 AnchorChip Bruker Mini 96 maldi plates Need to order adapter to put the mini plate in standard carrier from Bruker Part number 226413 MTP MSP adapter ABI Microtiter format Opti TOF sample plates 80 Applied Biosystems www agilent com chem 1200 Agilent Technologies Inc 2010 ae Published in USA July 1 2010 ogee Publication Number 5990 6115EN pr Agilent Technologies
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Agilent 1260 Infinity Micro scale Fraction Collector/Spotter handbook