Agilent 2100 Bioanalyzer Kits Reagents Data Sheet
Contents
1. 300 samples kit DNA 12 chip 300 samples kit DNA 12 chip 300 samples kit Determined analyzing the DNA ladder as sample Protein Analysis Kits and Reagents Order No Description Order No Description Order No Description 5067 1515 Agilent Protein 80 Kit 5067 1517 Agilent Protein 230 Kit 5067 1575 High Sensitivity Protein 250 Kit 5067 1516 Agilent Protein 80 Reagents 5067 1518 Agilent Protein 230 Reagents 5067 1576 High Sensitivity Protein 250 Reagents 5067 1577 High Sensitivity Protein 250 Labeling Reagents 5067 1578 High Sensitivity Protein 250 Ladder Analytical Specifications Agilent Protein 80 Assay Agilent Protein 230 Assay Agilent High Sensitivity Protein 250 Assay Sizing range 5 80 kDa 14 230 kDa 10 250 kDa Typical sizing resolution 10 10 10 Typical sizing accuracy 10 CV CAII BLG 10 CV BSA CAII 10 CV BSA Sizing reproducibility 3 CV CAII BLG 3 CV BSA CAII 3 CV BSA Sensitivity signal noise gt 3 6 ng pL CAII 15 ng pL BSA in PBS 6 ng pL CAII 15 ng pL BSA in PBS 1 pg L labeled BSA in water on chip 10 ng pL CAII in 0 5 M NaCl 30 ng L BSA in 0 5 M NaCl 5 pg L labeled BSA in PBS on chip 30ng pL BSA in 0 5 M NaCl Labeling reaction at 1 ng L of total protein Quantitative range 60 2000 ng pL CAII in PBS 15 2000 ng pL CAII Up to 4 orders of magnitude 30 2000 ng uL BSA in PBS 0 3 to 3000 ng L BSA Qualitative range 6 4000 ng uL CAII and BLG 6 5000 ng pL CAII 15 5000 ng uL B2. Total RNA Assay mRNA Assay Total RNA Assay mRNA Assay Quantitative range 25 500 ng pL 25 250 ng pL 50 2000 pg pL of purified miRNA in water Qualitative range 5 500 ng pL 25 250 ng pL 50 5000 pg pL 250 5000 pg pL 50 2000 pg pL in water in water of purified miRNA in water Sizing range 6 150 nt Sensitivity 5 ng pL in water 25 ng uL in water 50 pg pL in water 250 pg pL in water 50 pg pL in water signal noise gt 3 200 pg uL in TE 500 pg pL in TE Quantitation 10 CV 10 CV 20 CV 20 CV 25 CV reproducibility within a chip within a chip within a chip within a chip within a chip Quantitation accuracy 20 CV 20 CV 30 CV 30 CV Maximum sample 100 mM Tris 100 mM Tris 50 mM Tris 50 mM Tris 10 mM Tris buffer strength 0 1 mM EDTA 0 1 mM EDTA 0 1 mM EDTA 0 1 mM EDTA 0 1 mM EDTA or 125 mM NaCl or 125 mM NaCl or 50 mM NaCl or 50 mM NaCl or 15 mM MgCl or 15 mM MgCl or 15 mM MgCl or 15 mM MgCl Physical Specifications Analysis time 30 minutes 30 minutes 30 minutes 30 minutes 30 minutes Samples per chip 12 12 11 11 11 Sample volume Tul Tul 1 uL 1 uL Tul Kit stability 4 months at 4 C 4 months at 4 C 4 months at 4 C 4 months at 4 C 4 months at 4 C 25 chips per kit RNA Nano 12 chip RNA Nano 12 chip RNA Pico 11 chip RNA Pico 11 chip 300 samples kit 300 samples kit 275 samples kit 275 samples kit Determined analyzing the RNA ladder as sample Measured for the 40 nt fragment of the Smal
3. 8 o a Kits and Reagents for the ce o Agilent 2100 Bioanalyzer a 3 m Agilent offers a broad range of analysis DNA size and quantity smart high o kits for the 2100 bioanalyzer resolution separation and quantifica is tion of DNA e On chip flow cytometry the easy way to acquire dual color cell based SDS PAGE replacement for Protein fluorescence data analysis the fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity e RNA quality check the industry standard for RNA analysis offering total RNA and mRNA data with RNA Integrity Number RIN and Small RNA analysis Cell Analysis Kits and Reagents Order No Description Physical Specifications Agilent Cell Kit 5067 1519 Agilent Cell Kit Analysis run time 30 minutes 5067 1520 Agilent Cell Checkout Kit Number of samples 6 Sample volume 10 pL recommended number of cells 2 x 104 Kit stability 4 months at 4 C Agilent RNA Analysis Kits and Reagents Order No Description Order No Description Order No Description 5067 1511 RNA 6000 Nano Kit 5067 1513 RNA 6000 Pico Kit 5067 1548 Small RNA Kit 5067 1512 RNA 6000 Nano Reagents 5067 1514 RNA 6000 Pico Reagents 5067 1549 Small RNA Reagents 5067 1529 RNA Nano Ladder 5067 1535 RNA Pico Ladder 5067 1550 Small RNA Ladder Analytical RNA 6000 Nano RNA 6000 Nano RNA 6000 Pico RNA 6000 Pico Small RNA Specifications
4. SA in PBS Quantitation reproducibility 20 CV CAII BLG 20 CV BSA CAII 20 CV BSA Physical Specifications Analysis time 30 minutes 25 minutes 30 minutes Samples per chip 10 10 10 Sample volume 4 uL 4 uL Sul Kit stability 4 months see box for storage temp 4 months see box for storage temp 6 months at 20 C CAII Carbonic Anhydrase BSA Bovine Serum Albumin BLG beta Lactoglobulin www agilent com chem labonachip Copyright 2008 Agilent Technologies Published June 1 2008 Publication Number 5989 8803EN a Agilent Technologies
5. l RNA ladder Agilent Technologies DNA Analysis Kits and Reagents Order No Description Order No Description Order No Description 5067 1504 Agilent DNA 1000 Kit 5067 1506 Agilent DNA 7500 Kit 5067 1508 Agilent DNA 12000 Kit 5067 1505 Agilent DNA 1000 Reagents 5067 1507 Agilent DNA 7500 Reagents 5067 1509 Agilent DNA 12000 Reagents Analytical Specifications Agilent DNA 1000 Assay Agilent DNA 7500 Assay Agilent DNA 12000 Assay Sizing range 25 1000 bp 100 7500 bp 100 12000 bp Sizing resolution 25 100 bp 5 bp 100 1000 bp 5 100 1000 bp 5 100 500 bp 5 1000 7500 bp 15 1000 12000 bp 10 500 1000 bp 10 Sizing accuracy 10 CV 10 CV 15 CV Sizing reproducability 5 CV 5 CV 5 CV Quantitation accuracy 20 CV 20 CV 25 CV Quantitation reproducibility 25 500 bp 15 CV 100 1000 bp 10 CV 100 1000 bp 15 CV 500 1000 bp 5 CV 1000 7500 bp 5 CV 1000 12000 bp 10 CV Qualitative range 0 1 50 ng pL 0 1 50 ng pL 0 1 50 ng pL Maximum salt 250 mM for KCI 250 mM for KCI 250 mM for KCI concentration 250 mM for NaCl 250 mM for NaCl 250 mM for NaCl in sample 15 mM for MgCl 15 mM for MgCl 15 mM for MgCl Physical Specifications Analysis time 35 minutes 30 minutes 30 minutes Samples per chip 12 12 12 Sample volume Tul Tul Tul Kit stability 4 months see box for storage temp 4 months see box for storage temp 4 months see box for storage temp 25 chips per kit DNA 12 chip
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Agilent 2100 Bioanalyzer Kits Reagents Data Sheet