Agilent Multimode Source - Simultaneous ESI APCI for maximum throughput Manual
Contents
1. Agilent Multimode Source Simultaneous ESI and APCI for maximum throughput Simultaneous ESI and APCI for maximum throughput The Agilent multimode source for the LC MSD is a breakthrough in LC MS technology delivering simultaneous electrospray ionization and atmospheric pressure chemical ionization classical spectra and excellent sensitivity It maximizes throughput by eliminating the need to run samples twice to ensure all components are identified Eliminate the guesswork and reanalysis Not every compound responds to every ionization technique Some compounds ionize only by electrospray ionization ESI Other compounds respond only to atmospheric pressure chemical ionization APCI When you analyze unknowns you do not know ahead of time which mode to choose Even when looking for target com pounds you need to know if an unexpected impurity is present To be sure you must exchange sources and reanalyze The Agilent multimode source is the only ion source capable of simultaneously acquiring ESI and APCI data making reanalysis a thing of the past Separate zones simultaneous ionization ESI and APCI are very different processes and require very different conditions for optimum performance Only a truly revolutionary source could accomplish simultaneous high performance ESI and APCI The innovative Agilent solution is a single source with two separate opti mized zones one for ESI and one for APCI lons from b2. D D O D ea Sulfamethoxazole D D D D D D Tolazamide D D D D D D Uracil i SC Detected by polarity 67 56 67 50 78 61 Detected by source 89 89 100 D compound detected No compromises Some sources try to provide an The Agilent multimode source makes approximation of true simultaneous no such compromises It can acquire acquisition by switching back and data in both ionization modes 100 forth between ESI and APCI modes of the time There is no loss of data After subtracting the switching dead and sensitivity in mixed simultane time a switching source scans less ous acquisition mode is very close than 50 of the time in each ionization to the sensitivity provided by mode The inevitable result is a loss Agilent s state of the art individual of data especially when doing fast ESI and APCI sources chromatography with narrow peaks A T 1 pg reserpine 1200 S N 36 800 400 16 18 20 22 24 26 min Multimode source 18000 ESI mode 14000 S 10000 6000 16 18 20 22 24 26 min Multimode source 7000 Mixed mode 1 pg reserpine 5000 S N 36 3000 i 16 18 20 22 24 26 min Operating in the mixed ionization mode entails minimal loss of sensitivity Crystal violet Vitamin D3 372 3 9974 Dedicated ESI source Dedicated APCI source 2 E sa SS EEE E EAE SETE CEER NM se a a ies 100 200 300 400 m z 100 300 400 m z Multimode source 3723 gt Multimode source 397 4 mixed mode 2 mixed mode 2 simultaneo
3. oth ionization modes are analyzed simultaneously by the mass spectrometer 0 486 Dedicated ESI Source Crystal violet Vitamin D3 0 25 0 5 0 75 1 1 25 1 5 1 75 min Dedicated APCI Source 1 485 Vitamin D3 Crystal violet 0 25 0 5 0 75 1 1 25 1 5 1 75 min Multimode Source 1 488 ESI APCI Crystal violet Vitamin D3 0 25 0 5 0 75 1 1 25 1 5 1 75 min Simultaneous ESI and APCI maximizes throughput by eliminating the need to exchange sources and reanalyze samples Multimode source sk incorporates opti HPLC inlet Nebulizer mized ESI and APCI zones ESI zone APCI zone Thermal container Capillary Drying gas Polarity switching extends coverage APCI Source ESI Source Multimode Source The Agilent multimode source can Compound Positive Negative Positive Negative Positive Negative switch ion polarities on a scan to Acetazolamide EEN EE o CD scan basis With this rapid polarity Butyl 4 aminobenzoate D M amp T D foe p m switching you can acquire positive Cortisone D D D EEE o D and negative ESI and APCI dataina Gemfibrozil EE gt EE o EE o single run significantly increasing Hexahydro dione D EEE o E o CTE coverage over single mode ion Hydroflumethiazide EE o EE o E o sources Indole D E O lodipamide E o D D Labetalol D D D D D D Lidocaine oD E 0 E O Morin D D D D D D Paclitaxel u O M o Phenylbutazone D D D D D D Procainimide D E Oo E O Progesterone
4. s office or authorized Agilent Technologies distributor You can also visit our site on the World Wide Web at www agilent com chem This information is subject to change without notice Agilent Technologies Inc 2005 Printed in the USA February 24 2005 5989 2368EN r l a Agilent Technologies
5. us ESI APCI simultaneous ESI APCI 100 200 300 X 400 m z 100 300 400 m z Spectra from the multimode source are indistinguishable from those generated on dedicated sources Familiar spectra The Agilent multimode source delivers spectra identical to the spectra from dedicated sources In cases where a compound responds well to both ionization modes the electrospray process dominates but you can oper ate the multimode source in ESI only and APCl only modes if you want to obtain a specific type of mass spec trum Accommodates a wide range of LC flows Powerful infrared emitters in the Agilent multimode source provide tremendous drying capacity so you never need to compromise on LC flow rate The multimode source can accommodate anything from 1 mm columns at 50 pL min to 4 6 mm columns at 2 mL min There is no need for tricky unreliable flow split ting even at 2 mL min of 100 percent water Reduced drying gas consumption The same infrared emitters that help the multimode source accommodate a wide range of LC flows also drasti cally reduce drying gas consumption At high flow rates the Agilent multi mode source consumes less than half the drying gas of a dedicated ESI source For more information For more information about the Agilent multimode source call toll free 1 800 227 9770 U S and Canada In other countries please call your local Agilent Technologies analytical sale
Download Pdf Manuals
Related Search
Agilent Multimode Source Simultaneous ESI APCI for maximum throughput Manual