Agilent products for solid phase extraction Manual
Contents
1. Agilent Products for Solid Phase Extraction Improve the quality of your sample preparation so you can improve the quality of your analysis The Measure of Confidence 2 Agilent Technologies Table of Contents The Bond Elut Difference sisi scscccestscescosascsstucentsicontssost ten tjnoi dmantamesie 3 Polymeric SPE iiinis arannana aaraa 5 Silica Based SPF 8 Reversed Phase Non Polar Silica SPE 8 Normal Phase Polar Silica SPE 10 lon Exchange Silica SPE 12 Mixed Mode Silica SPE csssssssssssssssssssssssssssetssetetesseetn 16 Inorganic SPE isisisi eanais 18 Specia E 19 Diatomaceous Earth Sorbents for SUE see 23 Pak SPE a E 24 B nd Elut ACCOSSOri S nonoman 25 Agilent QuEChERS Kits Save time and money with a simplified approach to preparing samples With the largest compendium of QuEChERS applications available today easy kit selection and anhydrous salt packets Agilent has a solution for all of your QUEChERS needs Additionally increase homogenization efficiency and reduce shaking steps to only seconds with Agilent QUEChERS kits with ceramic homogenizers Learn more at www agilent com chem quechers Bond Elut The Original Packed Bed SPE Cartridge With the addition of Varian Agilent strengthens its commitment to unmatched quality innovation and services while offering an expanded portfolio of products services and support The extension of our instrument columns and supplies2. e Innovative Products Designed for Lab Efficiency Whether it be fast flow polymeric particles or our patented 96 well plate design all Bond Elut products are created for ease of use and flexibility to meet both manual and automated requirements e Technical Support at Every Step For your specific applications or to help solve occasional technical issues a global team of analytical scientists are on hand to assist e World Class Manufacturing and Quality Unrivalled manufacturing control plus exacting ISO 9001 2000 compliant inspections guarantee the consistent quality of Bond Elut The full Bond Elut portfolio will become available from Agilent November 1 2010 Sorbent Specifications Sorbent Phase AccuCAT Aminopropyl NH2 Carbon C18 CBA Certify Certify II AL PBA Plexa Plexa PCX Category Mixed Mode Polar Anion Exchanger Strongly Non polar Non polar Cation Exchanger Mixed Mode Mixed Mode Anion Exchanger Polar Covalent Polar enhanced Cation Mixed Mode Cation Exchanger Anion Exchanger Cation Exchanger Anion Exchanger Polar Bonded Functional Endcapped Group Base Material Sulfonic acid SCX and quaternary amine SAX silica based Aminopropyl silica based Graphitized carbon Trifunctional octadecyl silica based Carboxylic acid silica based Octyl and benzenesulfonic acid SCX silica based Octyl and quaternary amine SAX silica based Diethylaminopro
3. es Qo si CH lt _ S0 H CH 3 lon Exchange Silica SPE lon exchange phases are more dependent on pH ionic strength and counter ion strength than on solvent strength These phases depend on ionic interactions as the primary retention mechanism Bond Elut SCX e Useful for compounds with both cationic and non polar characteristics e Superior clean up from a single sorbent e Very low pKa ligand elicits strong analyte interaction Bond Elut SCX is a strong cation exchanger with a very low pKa Although the pKa is similar to Bond Elut PRS the presence of the benzene ring in the functional group increases the potential for non polar interactions This non polar characteristic becomes particularly important when conducting ion exchange from aqueous systems where selectivity towards compounds exhibiting cationic and non polar character is seen Bond Elut SCX Description Unit Part No LRC Cartridges 100 mg 10 mL 50 pk 12113013 500 mg 10 mL 50 pk 12113039 Straight Barrel Cartridges 100 mg 1 mL 100 pk 12102013 500 mg 3 mL 50 pk 12102040 500 mg 3 mL 120 um 50 pk 14102040 1g 6 mL 30 pk 12256011 Bond Elut Jr 500 mg 100 pk 12162040B 12 Bond Elut SAX e Retains compounds that elute from weak anion exchange sorbents e Selectivity can be user modified for increased flexibility e Minimal non polar interactions Bond Elut SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds such
4. Chem Elut Cartridges Volume mL Unit Part No Unbuffered 0 3 100 pk 12198001 1 100 pk 12198002 3 100 pk 12198003 5 100 pk 12198006 10 100 pk 12198007 20 100 pk 12198008 50 50 pk 12198009 100 25 pk 12198010 9 0 Buffered pH 3 100 pk 12198005 23 S a 2 S z d lt 2 LL S E 5 E SE O Chem Elut 12198001 Chem Elut 12198005 Bulk SPE and Accessories Bondesil Bulk Sorbents e Ideal for dispersive clean up techniques e Advanced bonding offers reproducible batch to batch performance e Multi kilo quantities available upon request Bondesil Bulk Sorbents Description Particle Size pm Unit Part No C18 40 100 g 12213012 120 100 g 14213012 NH2 40 10g 12213020 40 1000 g 12213021 PSA 40 10g 12213023 40 100 g 12213024 40 1000 g 12213025 Bond Elut Empty SPE Cartridges e Made with high purity polypropylene for cleaner extracts e Uniform batch to batch size for consistent performance e Economical for every day use A variety of empty reservoirs is available for packing of custom SPE cartridges with bulk Bondesil sorbents or other desired sorbent Cartridges are available from 1 to 60 mL Order frits separately or see below for reservoirs with pre installed frits Bond Elut Empty SPE Cartridges Volume mL Unit Part No 1 100 pk 12131007 3 100 pk 12131008 6 100 pk 12131009 12 100 pk 12131010 20 100 pk 12131011 60 100 pk 12131012 24 Bond Elut Empty SPE Cartridges with Two Frits e Pre installed frits for
5. as carboxylic acids which may not retain effectively on weak anion exchange sorbents Bond Elut SAX Description LRC Cartridges 500 mg 10 mL Straight Barrel Cartridges 100 mg 1 mL 100 mg 1 mL 500 mg 3 mL 1g 6 mL 29 12 mL Bond Elut Jr 500 mg Unit 50 pk 100 pk 500 pk 50 pk 30 pk 20 pk 100 pk 13 Part No 12113043 12102017 52102017 12102044 12256013 12256021 12162044B CH Ch Gd 0 si ch N CH cH cH E BEER H C Bond Elut PRS Strong cation exchange sorbent also capable of polar and hydrogen bonding interactions e No appreciable non polar interactions e Unique selectivity properties Bond Elut PRS is a strong cation exchange sorbent that is also relatively high in polarity With no appreciable degree of hydrophobicity in non polar solvents PRS is capable of polar and hydrogen bonding interactions Due to the very low pKa of PRS it is recommended for weaker cationic species such as pyridinium compounds Bond Elut PRS Description Unit LRC Cartridges 500 mg 10 mL 50 pk Straight Barrel Cartridges 500 mg 3 mL 50 pk 1g 6 mL 30 pk 14 Part No 12113038 12102039 12256010 Bond Elut PSA Alternative choice to Bond Elut NH2 for polar compounds CH e Higher ionic capacity than NH2 Q 0 Si CH N H N H CH 3 Bond Elut PSA is an alkylated amine sorbent that contains two different amino functionalities one secondary and one primary
6. mg 1 mL 100 pk 126418 250 mg 6 mL 30 pk 12102201 500 mg 6 mL 30 pk 12252201 Bond Elut Carbon NH2 Description Unit Part No Straight Barrel Cartridges 250 250 mg 3 mL 50 pk 121020410250 300 500 mg 6 mL 30 pk 2264265032 500 500 mg 6 mL 30 pk 12252202 500 500 mg 20 mL 20 pk 3664325032 Bond Elut Carbon PSA Description Unit Part No Straight Barrel Cartridges 250 250 mg 3 mL 50 pk 12102042C250 500 500 mg 6 mL 30 pk 12102042C500 19 Bond Elut AccuCAT e SCX and SAX functionalities offer broad analyte extraction potential Ultra clean mixed sorbent bed delivers for reproducible extractions e Compatible with many biological fluids for easy method transfer Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange SCX and a strong anion exchange SAX sorbent packed into one bed AccuCAT is effective for the extraction of acidic basic and neutral analytes from urine and other biological samples AccuCAT is particularly effective for catecholamine extraction from bio fluids Bond Elut AccuCAT Description Unit Part No Straight Barrel Cartridges 200 mg 3 mL 60 pk 12282003 Bond Elut PBA e Unique phenylboronic acid sorbent e High specificity for cis diol compounds e Amenable for a broad range of bio molecule applications Bond Elut PBA is a unique silica SPE sorbent containing a phenylboronic acid functionality that can retain analytes via a reversible covalent bond
7. 8 but less polar than C2 or CN Alkyl side chains confer moderately non polar characteristics Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties but with a slightly lower capacity as an anion exchange sorbent DEA has a moderately non polar character due to the alkyl side chains on the amino functionality These groups still afford a medium level of polarity higher than C8 but less polar than C2 or CN E Bond Elut DEA Description Unit Part No Straight Barrel Cartridges 50 mg 1 mL 50 pk 12102142 22 Hydromatrix and Chem Elut e High purity sorbent supported liquid extraction SLE applications Available in pre packed cartridges or bulk e Packing method delivers excellent tube to tube reproducibility Hydromatrix is a high purity inert diatomaceous earth sorbent available in 96 well plate formats and also as bulk material offering end user flexibility and an excellent diversity of applications Chem Elut is an economical broad performance sorbent for rapid general sample preparation of biological samples such as plasma serum whole blood and urine Chem Elut products are available in buffered and unbuffered formats The buffered devices can be used for simple scrubbing operations on organic reactions The base treated cartridge can remove residual acid compounds from a variety of matrices Hydromatrix Description Part No Hydromatrix bulk material 1 kg 198003 Hydromatrix bulk material 4 kg 198004
8. DIC Method optimized for acids 1 Conditon 500 uL CH30H followed by 500 uL water 2 Apply 100 uL plasma diluted 1 3 with 1 formic acid 3 Wash 500 uL 5 CH30H 4 Elute 500 pL CH30H Volumes stated are for a Plexa 30 mg plate pKa NEUTRAL BASIC Method optimized Mixed mode method optimized for neutrals and bases for bases 1 Conditon 500 ul CH30H followed 1 Conditon 500 uL CH30H followed by 500 uL water by 500 uL water 2 Apply 100 uL plasma diluted 1 3 2 Apply 100 uL plasma diluted 1 3 with 2 NH3 with 2 H3P04 3 Wash 500 pL 5 CH30H 3 Acidic wash 500 pL aqueous 2 formic acid 4 Neutral wash 500 pL CH30H CH3CN 1 1 v v 5 Elute 500 pL CH30H CH3CN 5 NH3 28 30 4 Elute 500 pL CH30H Bond Elut Plexa PCX e Faster flow rates improve productivity Extraction cleanliness and reduced ion suppression improves precision Simplified single method for ease of use Bond Elut Plexa PCX is a further milestone in the development of simple and robust SPE methods Plexa PCX uses a polymeric cation exchange resin that combines the outstanding properties of Bond Elut Plexa superior flow characteristics and improved analytical performance with strong cation exchange functionalities This mixed mode SPE sorbent removed neutral and acidic interferences from the matrix concentrates basic analytes and therefore improves sensitivity in the determination of basic compounds The Plexa PCX pa
9. This gives a slightly higher pKa and ionic capacity compared to Bond Elut NH2 PSA has a significantly higher carbon load than most amino functional sorbents and thus is a better choice for polar compounds which retain too strongly on Bond Elut NH2 Bond Elut PSA Description Unit Part No LRC Cartridges 500 mg 10 mL 50 pk 12113041 Straight Barrel Cartridges 500 mg 6 mL 50 pk 12102042 Bond Elut Jr 500 mg 100 pk 12162042B Bond Elut CBA Cation exchange with no need for extreme basic conditions CH 0 e Wider selectivity range provides more eluent options Q 0 Si CH CG e Polar or non polar depending on matrix or solvent CH CBA is a mid polarity sorbent and weak cation exchanger pKa 4 8 It can be used with a wider range of counter ions than lower pKa sorbents like SCX and will demonstrate easier elution of quaternary amine functionalized analytes Bond Elut CBA Description Unit Part No Straight Barrel Cartridges 100 mg 1 mL 100 pk 12102011 500 mg 3 mL 50 pk 12102038 15 Mixed Mode Silica SPE Bond Elut Certify e Special mixed mode sorbent bed e Broad application range for aqueous extraction e Bimodal non polar and strong cation exchange The Bond Elut Certify extraction cartridge utilizes a packed bed consisting of a special non polar C8 sorbent and a strong cation exchanger SCX Certify is most commonly used to extract basic cationic drugs from urine and blood but it is also very effective for ex
10. This very strong covalent retention mechanism enables high specificity and cleanliness The boronate group has a strong affinity for cis diol containing compounds such as catechols nucleic acids some proteins carbohydrates and PEG compounds Aminoalcohols alpha hydroxy amides keto compounds and others can also be retained Bond Elut PBA Description Unit Part No Straight Barrel Cartridges 100 mg 1 mL 100 pk 12102019 20 Bond Elut Mycotoxin Simple methodology saves time and increases throughput e Use with a broad range of food matrices e Economic and time saving alternative to immunoaffinity techniques Bond Elut Mycotoxin is a novel sorbent which cleans up food extracts for improved trichothecene and zearalenon analysis Results are comparable or superior to competing methods including immunoaffinity columns IAC and charcoal alumina column The sorbent is a proprietary silica based ion exchange material The Bond Elut Mycotoxin method for extraction and clean up has been successful with a variety of food and grain sample types including wheat corn durum oats bread muesli and infant food Bond Elut Mycotoxin is easy to use and acts in a selective non retention mechanism the toxin analytes pass through the cartridge while the food matrix components are retained Bond Elut Mycotoxin Description Unit Part No 500 mg 3 mL 50 pk 12102167 21 Bond Elut DEA e Weak anion exchanger e More polar than C
11. d amide free surface and a non polar PS DVB core for retaining small molecules Binding of proteins and lipids on the polymer surface is minimized resulting in cleaner samples and reduced ion suppression Plexa is therefore ideal for high throughput assays requiring validated performance with minimal method development The standard non polar retention mechanism is applicable to almost any analyte type and the performance features operate at the sample loading step making them largely method independent Bond Elut Plexa Description Unit Part No Bond Elut Plexa Cartridges 30 mg 1 mL 100 pk 12109301 30 mg 3 mL 50 pk 12109303 60 mg 1 mL 100 pk 12109601 60 mg 3 mL 50 pk 12109603 200 mg 3 mL 50 pk 12109610 200 mg 6 mL 30 pk 12109206 Bond Elut Plexa 96 Round well Plates 10 mg 1 mL 1 pk A4969010 10 mg high flow 1 pk A4969010HF 30 mg 1 mL 1 pk A4969030 30 mg high flow 1 pk A4969030HF Mega Bond Elut Plexa 500 mg 6 mL 30 pk 12259506 Comparison of particle sizes of non polar SPE Comparison of particle size distributions polymers by imaging analysis of non polar SPE sorbents oO oO Bond Elut Plexa ee Sy Other CX resin d Alternative Polymer Particle diameter um The narrow particle size distribution offers reproducible superior flow characteristics with minimal clogging General acid basic screening using Bond Elut Plexa and Plexa PCX Bond Elut Plexa PCX Bond Elut Plexa 2 ACI
12. ease of use e Broad range of filtration operations for maximum flexibility e Customizable packing for specific applications These clean polypropylene reservoirs contain two polypropylene frits pre inserted an ideal configuration for simple filtration For custom sorbent packing additional frits can be purchased separately Available from 1 to 60 mL Bond Elut Empty SPE Cartridges with Two Frits Volume mL Unit Part No 1 100 pk 12131013 3 100 pk 12131014 6 100 pk 12131015 12 100 pk 12131016 20 100 pk 12131017 60 100 pk 12131018 20 um Polypropylene Frits for SPE Cartridges e Made with high grade clean polyethylene for clean extracts Pre cut to correct size for accuracy e Use with reservoirs or custom packing These frits are pre cut to fit into Bond Elut reservoirs for use in filtration applications or for custom SPE sorbent packing 20 um Polypropylene Frits for SPE Cartridges Diameter mm Volume mL Unit Part No 6 4 1 100 pk 12131019 9 5 3 100 pk 12131020 12 7 6 100 pk 12131021 15 9 12 100 pk 12131022 20 6 20 100 pk 12131023 27 60 100 pk 12131024 25 Bond Elut Adapters e Connect SPE cartridges in series for large samples e Expand cartridge volume for even more applications Transfer large volume samples to any SPE cartridge Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge This allows the following configuration
13. imilar structure Applying the analytes in a non polar solvent then increasing the solvent polarity by increasing the concentration of a polar modifier such as THF or ethyl acetate delivers effective separations Bond Elut SI Description Unit Part No LRC Cartridges 500 mg 10 mL 50 pk 12113036 Straight Barrel Cartridges 100 mg 1 mL 100 pk 12102010 500 mg 3 mL 50 pk 12102037 1g 6 mL 30 pk 12256008 Bond Elut Jr 1g 100 pk 12166008B 10 Bond Elut NH2 Normal phase or anion exchange sorbent S 0 si ch NH CH e Weaker anion exchange than SAX e Amenable to separating structural isomers Bond Elut NH2 is a weaker anion exchanger than sorbents such as SAX a quaternary amine sorbent that is always charged and is therefore a better choice for retention of very strong anions such as sulfonic acids which may retain irreversibly on a SAX sorbent Similar to Diol an SI sorbents Bond Elut NH2 is excellent for the separation of structural isomers Bond Elut NH2 Description Unit Part No LRC Cartridges 200 mg 10 mL 50 pk 12113067 500 mg 10 mL 50 pk 12113040 Straight Barrel Cartridges 50 mg 1 mL 100 pk 12102076 100 mg 1 mL 100 pk 12102014 500 mg 3 mL 50 pk 12102041 1g 6 mL 30 pk 12256012 2g 12 mL 120 pm 20 pk 14256020 5g 20 mL 20 pk Bond Elut Jr 500 mg 50 pk 12162041B Bond Elut 96 Round well Plates 25 mg 1 mL A4960525 H 50 mg 1 mL A4960550 100 mg 1 mL A496051C j o 11 LH
14. in the Bond Elut range It is the most popular SPE sorbent because of its extreme retentive nature for non polar compounds C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents since it retains most organic analytes from aqueous matrices When analyzing small to intermediate molecules Bond Elut C18 can be used for desalting aqueous matrices prior to ion exchange as salts pass through the sorbent unretained Bond Elut C18 Description Unit Part No LRC Cartridges 200 mg 10 mL 50 pk 12113024 Straight Barrel Cartridges 100 mg 1 mL 100 pk 12102001 200 mg 3 mL 50 pk 12102025 500 mg 3 mL 120 um 50 pk 14102028 500 mg 6 mL 120 um 30 pk 14102052 2g 12 mL 20 pk 12256015 10 g 60 mL 16 pk 12256031 Bond Elut Jr 1g 100 pk 12166001B Bond Elut 96 Round well Plates 100 mg 1 mL A496011C LRC Large Reservoir Capacity CH E DEE CH Normal Phase Polar Silica SPE Normal phase sorbents are polar and used to retain extract polar analytes For normal phase sorbents retention decreases as the eluting solvent becomes more polar Bond Elut SI e Highly polar phase retains polar molecules from non polar matrices e High purity silica e Separate compounds with very similar structures Native silica is generally regarded as the most polar SPE sorbent available Bond Elut SI is particularly effective at separating compounds with a very s
15. portfolios allows us to offer you optimal solutions for your needs from one company Agilent can now offer you the best in class Bond Elut brand of products For over 30 years Bond Elut has been the most trusted name in solid phase extraction Years of use by demanding chemists at top companies worldwide have thoroughly documented its many applications and proven its performance To this day you will find more literature references for Bond Elut than any other SPE product in the industry Bond Elut is manufactured using state of the art automation to guarantee quality and consistency Optical scanners installed throughout our automated assembly process inspect each Bond Elut tube at multiple points And during manufacture 25 different tests are conducted to ensure reproducibility If an imperfection is spotted the tube is removed from the assembly line The result is consistently reliable Bond Elut cartridges time and time again The Bond Elut Difference Heritage of Reliability With years of use in some of the most demanding analytical laboratories in the world Bond Elut products have a proven track record resulting in a strong publication pedigree Options for Your Needs Offering extraction solutions for the widest range of analytes and matrices over 40 bonded silica phases for high specificity methods and polymeric phases for rapid method development Bond Elut has the largest choice of formats and sorbents in the market
16. pyl silica based Florisil Phenylboronic acid Hydrophilic styrene divinylbenzene SCX functionalized hydrophilic styrene divinylbenzene Propylsulfonic acid silica based Ethylenediamine N propyl silica based Benzenesulfonic acid silica based Trimethylaminopropyl silica based Silica Format Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Packed bed Typical Carbon Loading 7 0 Surface Area m2 g Particle Size pm and Shape 40 irregular 40 and 120 irregular 40 and 120 irregular 40 irregular 40 irregular 40 irregular 40 irregular 200 40 irregular 45 spherical monodisperse 45 spherical monodisperse 40 irregular 40 irregular 40 and 120 irregular 40 irregular 40 irregular Mean Pore Size A ERR A Polymeric SPE Bond Elut Plexa e Non polar retention mechanism e Improved extract cleanliness minimizes sample matrix interferences Simple methods are amenable to a very broad range of analytes Fast flow reproducible performance and ease of use Bond Elut Plexa offers simple easy to use methods with general purpose extraction mechanisms to simplify SPE In addition Plexa provides performance enhancements due to a unique polymeric architecture with a non retentive hydroxylate
17. risil is a magnesia loaded silica gel Like silica it is extremely polar in nature and ideal for the isolation of polar compounds from non polar matrices The larger particle size of the sorbent enables fast flow for large sample volumes and therefore can be an attractive alternative to silica if the sample matrix is particularly viscous Bond Elut Florisil Description Unit LRC Cartridges 500 mg 10 mL 50 pk Straight Barrel Cartridges 500 mg 1 mL 50 pk 1g 6 mL 30 pk 2g 12 mL 20 pk 5 g 20 mL 20 pk 18 Part No 12113049 12102050 12256014 12256022 12256030 Specialty SPE Bond Elut Carbon Excellent retention for small organics including those that are too polar to retain on C18 or polymeric SPE e Removal of chlorophyll and other pigments leads to fewer chromatographic or mass interferences e Broader retention and easier elution of analytes across the polarity range for improved multi residue analysis Bond Elut Carbon cartridges are packed with ultra pure graphitized carbon particles that have been optimized for the absorption of pigments in food fruits and vegetables and small organic residues in waste water The powerful retention mechanisms of these products are appropriate for a broad range of analytes In addition careful manufacturing techniques result in lower carbon fines on the wall of the device Bond Elut Carbon Description Unit Part No Straight Barrel Cartridges 50 mg 1 mL 100 pk 126414 100
18. rom solvent resistant high grade polypropylene are reusable and can be readily cleaned using organic solvents such as methanol or acetone Luer Stopcocks Description Part No Luer stopcocks 15 pk 12131005 Particle Size Specifications You will note that our most common silica based Bond Elut packings are described as 40 um materials yet if you look at the actual lot analyses you will see that the actual mean is around 55 um We have been making silica based Bond Elut packings since 1979 using the same diameter silicas in that time the models used to estimate irregular particle diameters and the testing equipment have changed We have retained the term 40 um however because there are so many official methods that specify a 40 um Bond Elut sorbent As other suppliers attempted to copy the successful Bond Elut product specifications the term has become an industry standard You can be assured however that the actual average particle in our regular silica Bond Elut is the same now as it was 30 years ago when we first pioneered SPE as a sample prep technology 27 A portfolio of solutions designed to give you ultimate confidence in your results Agilent sample preparation products improve the quality of your samples so you improve the quality of your analysis From solid phase extraction to industry leading instruments to quality columns Agilent has all the solutions to give you greater confidence in yo
19. rticles are near mono dispersed resulting in homogenous packing Reproducible results are the norm with very good tube to tube and well to well performance lon suppression is reduced because the highly polar hydroxylated polymer surface is entirely amide free and does not provide binding sites for endogenous species such as proteins and lipids Plexa PCX comes with a simple single method approach for basic drugs that offers improved recoveries cleaner extracts and reduced method development time and cost Flow rate is improved because Plexa PCX particles have much narrower particle size distribution with no fines to cause blockages Bond Elut Plexa PCX Description Unit Part No Bond Elut Plexa PCX Cartridges 30 mg 1 mL 100 pk 12108301 30 mg 3 mL 50 pk 12108303 60 mg 3 mL 50 pk 12108603 200 mg 6 mL 30 pk 12108206 500 mg 6 mL 30 pk 12258506 Bond Elut Plexa PCX 96 Round well Plates 10 mg 1 mL 1 pk A4968010 30 mg 1 mL 1 pk A4968030 Silica Based SPE Reversed Phase Non Polar Silica SPE Reversed phase sorbents are non polar and will be used to retain extract non polar analytes For reversed phase sorbents retention decreases as the solvent becomes more non polar Bond Elut C18 CH e Extremely retentive for non polar compounds Q 0 Si CH CH e Effective for desalting aqueous mixtures CH e The most hydrophobic bonded silica sorbent Bond Elut C18 is the most hydrophobic bonded silica sorbent available
20. s Bond Elut Adapter Configurations Configuration 1 The stacking of two cartridges in order to perform multi sorbent methods Configuration 2 3 Increasing any cartridge s volume by stacking an empty reservoir on top of the device Configuration 4 Standard Luer tipped syringes will fit into any Bond Elut adapter Gentle pressure can be then used to apply conditioning solvents samples rinsing solvents and eluents This configuration is particularly useful for single sample processing where a vacuum manifold is not required To select the appropriate adapter choose one specified for the size of the lower Configuration 5 For excessively large Bond Elut cartridge All Bond Elut sample volumes 1 8 in OD tubing cartridges and empty reservoirs have can be connected to the end of an standard Luer tips and are fully adapter and the sample can be drawn compatible across the adapter range directly from the sample container via a high vacuum Bond Elut Adapters Description Part No Adapter cap for 1 3 and 6 mL Bond Elut cartridges 15 pk 12131001 Adapter cap for 12 and 20 mL Bond Elut cartridges 10 pk 12131003 26 Luer Stopcocks e Control flow rates during SPE e Improve method reproducibility e Instant isolation from vacuum reduces accidental tube drying Luer stopcocks are used to provide independent flow control of each individual Bond Elut cartridge when used in conjunction with vacuum manifolds They are made f
21. traction of a wide range of compounds from a diverse range of aqueous matrices Bond Elut Certify Description Unit Part No LRC Cartridges 130 mg 10 mL 50 pk 12113050 200 mg 10 mL 50 pk 12113054 300 mg 10 mL 50 pk 12113052 Straight Barrel Cartridges 50 mg 3 mL 50 pk 12105030 100 mg 3 mL 50 pk 12102051 200 mg 3 mL 50 pk 12102145 300 mg 3 mL 50 pk 12102081 300 mg 6 mL 30 pk 12102082 1g 6 mL 30 pk 12102085 Bond Elut 96 Round well Plates 50 mg 1 mL A4960950 16 Bond Elut Certify II e Ideal for non polar and anionic compounds Optimized for acidic drug analysis e Bimodal non polar and strong anion exchange Bond Elut Certify Il was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices Certify II is a mixed mode cartridge packed with non polar C8 and strong anion exchange SAX sorbent It has been optimized for acidic drugs such as 11 nor delta 9 tetrahydrocannibinol carboxylic acid salicylic acid ibuprofen acetaminophen and other compounds that possess both non polar and anionic characteristics Bond Elut Certify Il Description Unit LRC Cartridges 200 mg 10 mL 50 pk Straight Barrel Cartridges 200 mg 3 mL 50 pk 7 Part No 12113051 12102080 Inorganic SPE Bond Elut Florisil e For clean up of polar interferences form non polar samples e Economical e Fast flow ideal for viscous samples Flo
22. ur results For more information Learn more www agilent com chem samplepreparation B uy online www agilent com chem store Find an Agilent customer center in your country www agilent com chem contactus U 1 a S and Canada 800 227 9770 gilent_inquiries agilent com Europe info_agilent agilent com Asia Pacific Information descriptions and specifications in this publication are subject to change without notice Agilent Technologies Inc 2010 e Printed in the USA July 28 2010 i ege 5990 6042EN eg dinquiry_aplsca agilent com d a Agilent Technologies
Download Pdf Manuals
Related Search
Agilent products for solid phase extraction Manual