Agilent Cary 100/300 Series UV-Vis Spectrophotometers brochure
Contents
1. Molecular Spectroscopy Solutions can deliver the performance accuracy Cary 60 UV Vis Cary 4000 5000 6000i Cary Eclipse Fluorescence Ln Spectrophotometer UV Vis NIR Spectrophotometer and flexibility you need Publication number 5990 7789EN Spectrophotometers Publication number 5990 7788EN e E Learn more www agilent com chem Buy online www agilent com chem store a Find an Agilent customer center in your country www agilent com chem contactus U S and Canada a fier 1 800 227 9770 agilent_inquiries agilent com te ia a Europe i a S a info_agilent agilent com all Molecular Spectroscopy f Asia Pacific Portfolio adinquiry_aplsca agilent com Publication number 5990 7825EN This information is subject to change without notice Agilent Technologies Inc 2011 Printed in U S A May 1 2011 5990 7785EN The Measure of Confidence atte Agilent Technologies2. complete qualification services 10 00 for the Agilent Cary 100 300 Series UV Vis hardware software and accessories Excellent temperature control The temperature controlled single or multicell peliter thermostatted cell holders offer e Excellent stability control over time typical variation 0 05 C e Minimal cell to cell variation maximum difference 0 2 C at 37 C e Accurately measure the temperature of the actual sample within the cuvette using temperature probes e In built electromagnetic stirring providing complete control of the stirring speed with no fluctuations up to 12 cells The leader in thermal melts Superior multi and single cell peltier temperature control allows temperature ramp rates as slow as 0 06 C min to be selected for high resolution DNA and protein thermal melting experiments Collect Temperatures Start C 15 0 Fs 25 0 Z C Simple Collect Advanced collect Temperature Monitor Probe 1 Return to C Number of Stages 4 200 95 00 200 Stag 200 15 00 200 0 50 95 00 5 00 gt el Unrivaled temperature control Select up to 20 thermal denaturation and renaturation ramps within one data file for annealing and thermal melt experiments in one vessel 20 40 60 80 29 3 cel 3 2 c 1000VT 1M 3 3 Superior signal to noise performance The excellent signal to noise performance of the Agilent Cary 100 300 Series UV Vis instruments means you can detect very
3. spreadsheet TE Advanced data processing Use the spectrum calculator to apply mathematical operations including addition subtraction division multiplication log and square root functions to spectra The calculator also features mean normalization smoothing up to fourth order derivatives integration and the Kubelka Munk correction algorithm Enhanced graphics features The graphics control module has automatic peak labelling zoom free and tracking cursor multiple ordinate and abscissa formats smart copy paste and overlay modes making spectral interpretation and presentation for publications a breeze Meet your application challenges Use the powerful built in Applications Development Language ADL to tailor the WinUV software to meet your most specific applications MATERIALS TESTING AND RESEARCH APPLICATIONS When you need to consistently and cost effectively deliver the highest quality finished products and materials innovative reliable analytical solutions are essential to your success The Agilent Cary 100 300 Series UV Vis provide unrivalled photometric accuracy and linearity across the broadest wavelength range combined with the widest and most versatile sampling solutions Thin Film Measurements The Agilent Cary 100 300 Series UV Vis with internal diffuse reflectance accessory DRA or VW specular reflectance accessory VW SRA accurately determines the thickness of thin film coatings in the UV
4. 00 1000 9 50 100 Min Concentration g L Signal to noise Signal to noise S N mode is a unique scanning mode available only on the Agilent Cary instruments that enables you to control the level of precision you want across the whole scan It is particularly useful for samples that vary significantly in either absorbance or reflectance across the wavelength range S N mode reduces scanning times by over 50 as the system scans quickly in areas of high energy throughput and increases signal averaging when energy throughput is less Reduced cost of ownership Premonochromator No peak shifts The sealed optics prevent extends range A phase locked wavelength exposure to corrosive The Agilent Cary 300 has a drive prevents peak shifts environments extending the premonochromator extending and peak suppression at high instrument s lifetime and its linear photometric range scan speeds reducing service costs typically past 6 0 absorbance units Abs The Agilent Cary 100 has a working range past 4 0 Abs A Variable slits Providing optimum control over spectral resolution Large sample compartment Giving you more flexibility in sample size Quartz overcoated optics Protects optics from the environment ensuring optical performance is maintained throughout the life of the instrument Choice of modes Although the Agilent Cary 100 and 300 are a double beam design they can operate in single double or d
5. Flexible Accurate Intuitive AGILENT CARY 100 300 SERIES UV VIS SPECTROPHOTOMETERS The Mealsure of Confidenc 3 2 Agilent Technologies ae SS J Answers you can trust The Agilent Cary 100 300 Series UV Vis spectrophotometers are flexible accurate and intuitive They are designed to meet your application requirements now and in the future With a variety of sampling options high sensitivity and superior photometric performance you can be sure that the Agilent Cary 100 300 Series UV Vis will give you answers you can trust Agilent Cary 100 190 900nm The Agilent Cary 100 double beam UV Vis with a working range past 4 0 Abs is ideal for routine and research laboratory work With variable slit widths it provides optimum control over data resolution Agilent Cary 300 190 900nm The Agilent Cary 300 double beam UV Vis with a working range past 6 0 Abs and resolution better than 0 24 nm is a research grade instrument with a pre monochromator making it ideal for the analysis of highly turbid biological samples or highly absorbing solid materials Molecular Spectroscopy Innovations 1947 1954 1969 1977 1979 First commercial Release of the First rapid Release of the First commercial recording UV Vis Cary 14 UV Vis NIR scanning fourier Cary 219 UV Vis diode array the Cary 11 UV Vis transform infrared spectrophotometer spectrometer the 8450A the FTS 14 1997 1999 2000 2002 2008 Cary 50
6. Series Launch of the First ATR chemical Cary 4000 5000 Launch of the released to Cary Eclipse imaging system 6000i research 600 Series FTIR coincide with Fluorescence grade UV Vis NIR_ spectrometers 50th anniversary Series series released microscopes and of Cary 11 imaging systems FOR YOUR APPLICATION 1989 1995 Release of the Launch of the acclaimed Cary 1 8453A the first and 3 UV Vis small footprint full featured diode array 2011 2011 Agilent offers Release of the out of lab FTIR Cary 60 UV Vis solutions Agilent is committed to providing solutions for your application We have the technology platforms and expert guidance you need to be successful SSA TESTING pee ERESEARCH ss CHEM amp PETROCHEM ACADEMIA Common applications Thin film measurements Analysis of heavy metals in water food for the Agilent Cary 100 Measurement of films multiple filters and agriculture DNA and protein quantification Measuring small volumes Color measurements and color matching ae of organics in water food and Monitoring kinetics of reactions that occur agriculture at sub second rates Common applications Manufacture and testing of optic Small volume measurements DNA and protein denaturation thermal melts for the Agilent Cary 300 components coatings High throughput liquid quantitation Measuring enzymatic reactions Refractive index EA OEE Powder and pigment analysis Measuring highly turbid biological samples ao of i W fa
7. Vis spectral region by measuring the number of interference fringes from the sample For calculating the refractive index RI of the sample or measuring thin film coatings in the Near IR region use the Agilent Cary 5000 or Cary 6000i VW Specular Reflectance Accessory VW SRA The VW SRA can be used with the Agilent Cary 100 300 UV Vis to accurately determine the film thickness N TAN AI NAN I NN N WY i II i T l zi ig T T T T q 300 350 400 450 500 550 600 650 700 750 800 Wavelength nm Fringes were used to determine the film thickness of a thin film coating on a polycarbonate substrate The thickness was calculated to be 4 52 um Solid sample measurements Use the solid sample holder for securing and positioning samples during UV transmission measurements of solids such as filters glass and textiles These versatile holders can be configured to cater to a range of sample sizes Measurement of films multiple filters Use the Agilent Cary 100 300 Series UV Vis with motorized sample transport accessory and film holder to determine surface homogeneity and or defect rates of films gels wafers or multiple filters e Eliminates time consuming manual adjustments reducing operational error and cost e Accurate and reproducible sample positioning in the sample compartment e Automated scanning capability is ideal for monitoring intra sample homogeneity and detecting sam
8. ctor Measuring suspensions and highly such as cytochrome P450 UPF of fabrics and clothing scattering samples Common sampling techniques Diffuse reflectance accessories Diffuse reflectance accessories Rapid mix accessory supported by the Agilent Cary internal and external internal and external Single cell peltier accessory 100 300 Fibre optic coupler accessory Fibre optic coupler accessory accurate temperature control 6x6 or 8x6 multicell holder accessories 6x6 or 8x6 multicell holder accessories Peltier multicell accessory Routine sampling accessory Fibre optic coupler accessory Diffuse reflectance accessories internal and external Temperature Probe accessory monitor temperature inside cuvette Micro volume cuvettes accurate QUALITY AND PERFORMANCE BY DESIGN Our proven record of optical design excellence and innovation ensures you get the right answer every time Superior linearity Excellent photometric stability The excellent linearity of the Agilent Cary 300 makes it ideal for The optical design of the Agilent Cary UV Vis instruments ensures measuring samples as high as 6 Abs accurately and with confidence superior photometric stability This means that any absorbance change you observe over time for example during a kinetics run is due only to the sample not to drift in the instrument 0 0010 0 0005 Abs S 0 0000 haspail a Are DATs a ars arpa been aibe Pe aa x 0 0005 0 0 0010 0 200 400 600 8
9. e thermostattable multicell holder consists of two staggered six cell holders which provide magnetic stirring in each of the 12 cells Use it in conjunction with the temperature probe to measure the temperature inside the cuvette DISTINCTLY BETTER SOFTWARE User friendly application focused software provides complete instrument control Software designed for real samples The modular design of the Cary WinUV software means that it can be tailored to suit your analytical requirements whether it s a materials science application using wavelength scanning measurements or life science applications requiring advanced enzyme kinetics or thermal control aozi Ge Q ES Tol a ar OS RES gee Se Tie E B 0 ee E a E nona ome wo mmn lim Em Li bm P inir A i ipik i Bs Bemi eie A AE ue oe ee eo ee a a E Ceo ae Se i ii fT m Ef F Dyki am m ee i E I r F a E i F it i iu E T b Primi e 2 eta ey alee ee Hara ee oa Ti Ta LETE OEM nE TET WE ip Oe MOD aan eal MEAS fet paii iS Ge Da hee 2 ed G e p eq 25 e ee E HE F ji FEH DEE wa Cui KE i i DWED Gee Oa j ifr ae Se Se See Dee Oe j a i ire ee Bee ee a Enhanced file transfer and report export capabilities The auto convert function allows you to convert your data files automatically for use in another program You can also elect to store your data files in formats that can be directly imported into a
10. ensity which typically makes it difficult to detect very small changes in absorbance The Agilent Cary 300 enables such measurements to be taken directly on whole cells directly from a cuvette 11 Trust Agilent to keep your lab running at peak productivity Agilent s Advantage Service protects your investment in Agilent instruments and connects you with our global network of experienced professionals who can help you get the highest performance from every system in your lab Count on us for the services you need at every stage of your instrument s lifecycle from installation and upgrade to operation maintenance and repair For customers who require full system validation Agilent offers complete qualification services Installation and Operational Qualification for the Agilent Cary 100 300 Series UV Vis hardware software and accessories And if ever your Agilent instrument requires service while covered by an Agilent service agreement we guarantee repair or we will replace your instrument for free No other manufacturer or service provider offers this level of commitment Further information For full details of the Agilent Cary range of molecular spectroscopy products ask for a brochure or visit our web site at www agilent com chem UV Our catalog of new applications Is ever growing To learn about the latest contact your local Agilent Representative or visit us at www agilent com chem Find out how Agilent s
11. ple defects e Able to accommodate multiple optical samples making it ideal for fast QA QC or accelerated R amp D applications requiring inter sample comparisons Solid sample measurements For rapid measurement of sheets films gels wafers or multiple filters DRA for diffuse transmission measurements Use the Agilent Cary 100 300 Series UV Vis with DRA or fibre optic reflectance probes to perform color measurement or color matching of liquids solids and pastes and samples that are too large to fit in the sample compartment Color Measurements Use the Agilent Cary 100 300 Series UV Vis with DRA or fibre optic reflectance probes to perform color calculations for liquids solids pastes or samples that are too large to fit in the sample compartment 400 500 600 700 800 Wavelength nm x White Yellow White Yellow Black 0 5 0 0 0 5 1 0 0 Blue a Blue a The optional Color Calculation software supports an extensive range of color calculations including Tristimulus Chromaticity CIE Lab CIE LUV and many more 10 trusiec j BIOTECH AND PHARMA APPLICATIONS In a field that demands accuracy productivity and regulatory compliance your challenges have never been greater The Agilent Cary 100 300 Series UV Vis provide unrivalled optical performance and superior temperature control to measure the most challenging samples with the highest accuracy Complete 10 00 services Agilent offers
12. small absorbance changes in thermal melt Tm curves with ease Monitor temperature control The Temperature probe accessory enables the temperature inside the cuvette to be measured and used to control the temperature rates of your experiment Advanced kinetics analysis Data collection rates can be varied to collect more data when you need it fast at the start up to 30 data points per second and then slower during the later part of the reaction The Kinetics software also accommodates long slow reactions and is capable of collecting data for up to 5 5 days without limiting the number of data points collected 0 8 06 fh 40 Abs 0 2 0 0 Sa 0 300 400 500 Wavelength nm Obtain kinetics curves easily With a mouse click you can obtain a kinetics curve from a series of repetitive curves The insert shows the kinetics curve at 410 nm Perform 1 experiment instead of 12 The Dwell Time option allows you to measure multiple data points per cell before moving to the next cell The insert is a zoomed section showing data points collected in less than 1 second 0 04 _ membranes 3 0 02 whole cells 0 00 I 420 430 440 450 460 470 480 A nm Measure highly turbid samples Baseline corrected scans of both whole cells and plasma membrane vesicles from a bacterial strain expressing low levels of cytochrome P450 are shown Such cultures are highly turbid suspensions with high background optical d
13. ual single beam modes to extend sampling capacity Accessory controller Superior optical design The accessory controller Double choppers ensure that offers centralized accessory the sample and reference control allowing you to beam strike the detector at control Agilent and common the same point eliminating third party accessories any errors due to non uniformity of the detector INTUTIV YOU CAN DO IT ALL WITH A CARY Agilent Cary 100 300 Series UV Vis spectrophotometers are complemented by a range of accessories Supplies and software designed specifically for your application needs Performance enhancing accessories The vast range of accessories for Agilent Cary 100 300 Series UV Vis ensures you can handle the widest variety of sample sizes and types Accessories for solids powders and pastes e Internal and external diffuse reflectance accessories DRA e Sample transport accessory and film holder e Solid sample holder Accessories for liquid samples e Diffuse reflectance accessories DRA e Multicell holder e Peltier temperature single and multicell holders e Polarizer and depolarizer e Rapid mix accessory e SPS 3 sample preparation system Autosampler e Routine sampler accessory water thermostatted or peltier controlled e Temperature probe Consumables for UV Vis e Agilent s range of UV Vis consumables includes cuvettes flow cells and lamps Control cell temperature from 10 C 100 C Th
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Agilent Cary 100/300 Series UV Vis Spectrophotometers brochure