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Agilent 500 Ion Trap LCMS

1. Agilent 500 lon Trap LC MS LC MS PERFORMANCE AND PRODUCTIVITY THROUGH INNOVATION The Measure of Confidence em Agilent Technologies The Agilent 500 lon Trap LC MS is the instrument of choice for high throughput LC MS analysis where sensitivity reliability and productivity are essential The Agilent 500 lon Trap LC MS is a new and innovative system that provides a robust platform for demanding LC MS MS applications The system sensitivity mass resolution and scan speed reflect the inherent advantages of the ion trap while the new Enhanced Charge Capacity ECC extends the number of ions that can be stored The results are greater sensitivity and reduced background noise for reliable quantitation The Agilent 500 lon Trap LC MS features a patented tilted capillary inlet and ion gating optics to ensure the lowest possible chemical noise while delivering premium detection levels and Productivity through Innovation By delivering excellent sensitivity over the entire mass range in full scan mode and exceptional MS MS and MS capabilities the Agilent 500 lon Trap LC MS quickly provides superior qualitative information for even the most complex sample matrices Unique sensitivity enhancements include SelecTemp the temperature programmable API Atmospheric Pressure lonization for the optimum analysis of thermally labile compounds These innovations lead to the remarkable performance and capabilities of the
2. Agilent 500 lon Trap LC MS 500 lon Trap LC MS With Seleclemp and Enhanced Charge Capacity State of the art API interface The Agilent 500 lon Trap LC MS delivers increased sensitivity and selectivity for all analytes Even highly thermally labile compounds can be reliably analyzed using Agilent s patented temperature programmable Seleclemp API interface Maintaining sample integrity Is no longer a concern Seleclemp s programmable temperature control delivers optimum drying gas temperatures at every time point throughout a complex analysis Additional SelecTemp API benefits include reduced interface contamination improved throughput simplified setup and automatically recorded parameters for GLP purposes The rugged yet elegant design of the SelecTemp API and its unique capabilities will improve the performance of routine high sample throughput applications reliably consistently and easily while Increasing accuracy and productivity ECC triple resonance and the power of MS S N PP 52 ECC ensures the reliable detection of trace amounts in the presence vA of high concentrations of coeluting E compounds and heavy matrices Patented triple resonance scanning allows scan speeds up to 15 000 Da sec and preserves chromatographic spectral resolution The fast scan speed is complemented by rapid ion polarity switching of 200 ms to und accommodate optimum detection i m of ion polarity for every analyte i
3. ange of special detection techniques including fluorescence refractive index or evaporative light scattering are also available for applications where non UV absorbing chromophores are analyzed 1200 Infinity Standard Poroshell Sub 2 um 4 6 mm system HPLC columns columns columns 1260 Infinity Binary LC 3 0 mm columns Position A Column 2 Eluent 10 pump Autosampler 1 Waste Regeneration Detector pump Column 1 Without Analysis 1 Regen Analysis2 Regen Analysis 3 alternating Dmm EE regeneration With r alternating Analysis 1 Regen Analysis 3 regeneration ay Analysis 2 Regen Higher throughputs can be achieved through alternating column regeneration ACR Absorbance mAU 35 30 25 20 Peak capacity 694 15 LC with GC resolution 5 10 15 20 25 30 40 Time min 35 Peak capacities of more than 700 can be achieved using a ZORBAX RRHT SB C18 column 2 1 x 150 mm 1 8 um to analyze a tryptic digest of BSA 2 1 mm columns Fast LC MS frontend High resolution Semi prep Unequaled ownership advantages The Agilent 500 lon Trap LC MS versatility and distinctive features allow easy adjustment to accommodate a wide variety of analytical requirements The standard electrospray ionization source ESI of the 500 lon Trap LC MS can easily be replaced with an optional atmospheric pressure chemical ionization APCI source in less than a minute LC flow rates f
4. er The integrated autosampler is designed for reliability safety and easy maintenance and facilitates reliable injections from 0 1 to 100 uL The unit delivers high precision as well as excellent linearity over a wide volume range The autosampler s flow through design ensures that there is no loss of sample the entire sample drawn from the vial Is injected The standard configuration offers a sample capacity of 100 2 mL vials alternatively two half trays each with 40 2 mL vials or 15 6 mL vials can be used Column oven The optimized column oven houses a single column and provides accurate stable column temperature from 10 degrees above ambient to 60 C for applications where retention time reproducibility and separation quality improve above ambient temperatures Variable wavelength detector Featuring simplified optics for high sensitivity low baseline drift wide linear range and 80 Hz data rate Agilent s variable wavelength detector not only lowers your detection limits and expands your capabilities it lowers your cost of ownership with easy front access for fast lamp and flow cell replacement Agilent s Software More power with Agilent MS Workstation software Intuitive instrument control and data processing are simple yet powerful with the Agilent MS Workstation software Effortless detector optimization and control e Superior data transfer rate with dual processors oimple navigation while rev
5. ibrary Five new Constraints have been added Tags in Comment Peptide Sequences Peptide Mobile Protons Peptide Charge and Peptide Number of Residues Tags in comment for example allows searching for the name of the contributor in the EI library Asearch for information in the Contributor Comment field which may be particularly useful for certain types of information relevant to MS MS Flexibility has been added to searches using a Name Fragment in Constraints It is now possible to specify the presence or absence of any arbitrary part or parts of a name he program does not use or require the installation of NIST fonts Column Options Maximize system performance and reliability for all your separation needs by combining your 1200 Infinity Series LC system with Agilent ZORBAX and Poroshell LC columns and LC supplies Designed with the same attention to detail quality and superior performance you have come to expect from Agilent instruments and backed by over 40 years of chromatography experience you can count on Agilent s columns and supplies to deliver the high quality results you need and expect time after time Unparalleled quality and flexible choices within the ZORBAX LC column family Wide selection of chemistries Eclipse Plus Eclipse XDB StableBond Extend Bonus RP HILIC and more superior flexibility and product scalability with a wide range of particle sizes 1 8 3 5 5 um and superficially por
6. iewing and processing results Full featured network compatibility for file management printing and remote access Extensive reporting for complete analyses Analyze identify and confirm with TurboDDS data dependent scanning TurboDDS is an advanced scanning form of the 500 lon Trap LC MS TurboDDS can be used to automatically gain qualitative information based on simple operator specified criteria The system performs MS MS and MS experiments in a single analytical run MS Workstation Key Benefits include Data dependent scanning Accelerate and simplify information capture from unknown samples Extended mass range Analyze large molecules up to 3500 m z High resolution scanning Accurately determine charge state of multiply charged ions up to charge state 6 Rapid auto CID scan Optimize CID up to 5 times faster than existing instruments to provide more comprehensive identification of unknowns Easy to use data review software Discover informative links between related data points Generate user libraries effortlessly Fast calculation Mass Ruler software tool measures mass difference between peaks with two mouse clicks e Easy data export Transfer results to third party databases such as Mascot and Phenyx to enhance information derived from the sample TurboDDS Data dependent scanning A simple structural elucidation with TurboDDS Sur
7. n the sample Enhanced qualitative information is delivered by the 500 lon Trap LC MS with MS operation to nz10 expanding the spectral information content for structure elucidation 500 fg Temazepam in BSA Bovine Serum Albumin ESI MS MS 301 gt 255 Doubly Charged lons Normal scan speed at 5000 Da sec Single Charged lons at Enhanced scan speed at 15 000 Da sec The Agilent 500 lon Trap LC MS delivers superior resolution at normal and enhanced scan speeds Agilent 1220 Infinity LC Simply more in tune with your lab s needs Take a close look at the Agilent 1220 Infinity LC System and you ll find everything you need for first class results and rewarding day after day productivity Choose from three standard configurations to fit your LC workflow and rest assured that you have made an investment that is truely future proof With a range of future options available you can choose an upgrade path that meets your lab s requirements today and tomorrow Move from isocratic to gradient solvent delivery from manual injector to autosampler or add a click in column oven Extend your detection capabilities with any 1200 Infinity Series detector such as diode array DAD evaporative light scattering ELSD fluorescence FLD or refractive index RID Upgrade your Agilent CDS software from a single workstation to a client server installation for enha
8. n is 57 Da MSn Path B The second most abundant product ion in the MS MS spectrum is m z 142 2 This is TBA minus one butyl side chain and one propyl group 100 0 The m z 142 2 ion is isolated and the MS analysis produces a product ion m z 100 2 This represents the loss of another propyl group 42 0 The m z 100 2 ion is isolated and the MS analysis creates a product ion m z 58 0 representing the loss of yet another propyl group 42 0 The intensity of the MS product ions meets the threshold requirement and the MS analysis proceeds However the generated product ions have extremely low ion intensities with limited significance MSn Path C he third most abundant product ion in the MS MS spectrum is m z 100 2 This is TBA minus one butyl side chain and two propyl groups 142 0 The m z 100 2 ion is isolated and the MS analysis produces a product ion m z 57 9 representing the loss of yet another propyl group 42 1 An MS analysis is not performed because the intensity of the MS product ions falls below the ion intensity threshold defined in the method _ NIST 08 and the MS Search Program P New Features in NIST 08 and the MS Search Program Version 2 0 of the MS Search Program The basic software of the NIST MS Search Program for Windows Version 2 0 is unchanged A number of new features have been added Anew spectrum search for MS MS spectra in MS MS libraries including the NIST MS MS l
9. nced system control and data handling Solvent delivery Based on the design used in the Agilent 1260 Infinity LC the pumping module ensures stable virtually pulse free solvent flow up to 10 mL min Servo controlled dual floating pistons enable 2 801 gs c c nN 1 388 2394 1 658 1 543 3248 8 102 23 138 32 941 1 325 22 805 28 384 29 489 c c Aw m 12 320 0 5 10 15 20 25 30 Time min M p D LC MS screening for EU banned dyes in textiles Total ion chromatogram TIC of a mixture of 22 aromatic amines operated in time programmed SIM mode variable stroke volume optimized for the selected flow rate and keeps maintenance to a minimum The pressure range up to 600 bar lets you discover the power and advantages of columns with smaller particles for optimization of resolution and faster run times Choose from different configurations including either an isocratic pump or a gradient pump with up to two solvents and integrated two channel degassing unit Pump options include Automated solvent selection valve for gradient pump combining two out of four solvents for gradient formation or for column flushing with different solvents Continuous seal wash kit based on the design of the Agilent 1260 Infinity pumps to prevent corrosion for applications running buffer solutions with high salt concentrations gt 0 1 M The gradient pump includes an integrated d
10. ous 2 7 um and column dimensions Individual column performance reports to document column to column and lot to lot reproducibility ouperior particle strength for even the most demanding high pressure applications Column technology to help you get the most from your 1260 Infinity LC Agilent Poroshell 120 Columns have a solid 1 7 um core and a 0 5 um porous outer layer so you get the resolving power of a 2 um particle at the speed of a sub 2 micron particle with significantly lower backpressure And they are manufactured with a proprietary single step porous shell process that has been designed by Agilent to improve reproducibility Poroshell 120 columns offer Upto two times the number of theoretical plats compared to conventional 3 5 um columns e Improved resolving power even as you take advantage of speed gains Improved ability to handle dirty samples due to their standard 2 um frit lo Poroshell 129 W pressure for HPLC UHPLC Polaris and Pursuit Polaris columns provide unique bonded phases that maximize polar retention and selectivity while virtually eliminating silanol activity Polaris ability to outperform the competition on challenging separations sets the standards for peak symmetry selectivity and reproducibility Pursuit diphenyl columns deliver faster separations without sacrificing resolution Moving through the analytical development process and into production Pursuit material
11. rom microliters to milliliters can be used with our fully adjustable electrospray ion source and the vortex atmospheric pressure chemical ionization source The novel design of the ion sources means the 500 lon Trap LC MS requires less cleaning leading to returns in up time and productivity The built in syringe pump diverter valve and Electronic Gas Control EGC provide ease of use benefits enhancing productivity even more Clearly the performance driven Agilent 500 lon Trap LC MS delivers increased selectivity sensitivity and accuracy while reducing contamination and downtime The resounding capabilities of the Agilent 500 lon Trap LC MS ultimately translate into value through exceptional results unparalleled economy and cost of ownership advantages second to none 11 Agilent 500 lon Trap LC MS For more information on Agilent Technologies 500 lon Trap please visit our website at www agilent com U S and Canada 1 800 227 9770 agilent_inquiries agilent com Europe info_agilent agilent com Asia Pacific adinquiry_aplsca agilent com The Measure of Confidence This information is subject to change without notice E Agilent Technologies Agilent Technologies Inc 2011 Printed in U S A April 5 2011 5990 8154EN
12. s meet requirements for resolution critical analytical methods and high quality preparative chromatography Infinitely better together with MS The 1260 Infinity Binary LC sets higher standards in performance and value to give you more confidence in your results The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity Its scalable delay volume makes it ideally suited for standard bore columns and as a front end for MS detection Further it comes at the price of a typical binary system for conventional HPLC Higher throughputs A special high throughput configuration of the 1260 Infinity Binary LC enables maximized productivity for laboratories with high sample loads While one column is used for the analysis the second column is washed and regenerated by a regeneration pump With this high throughput configuration you can reduce cycle times by up to 50 percent Use an 1290 Infinity LC Injector HTS or HTS for highest sample capacity and benefit from the full mix and match capabilities of the 1200 Infinity Series Full mix and match capabilities of the 1200 Infinity Series A wide range of autosamplers are available for a variety of tasks ranging from general HPLC needs up to dedicated solutions for high sample throughput UV visible detectors are offered with up to 10x higher UV sensitivity from programmable single wavelength to high speed multi wavelength and full spectral detection A r
13. ual channel degassing unit that creates continuous vacuum using the same Teflon AF technology used in the 1290 Infinity Binary Pump With low internal volumes of 1 5 mL per channel the degassing unit eliminates baseline fluctuations and shortens equilibration times ZORBAX Eclipse Plus C18 3 0 x 50 mm 40 1 8 um Absorbance mAU 0 123456 Time min Easy transfer of an HPLC method to RRLC by deploying a shorter column with sub 2 micron particles Both shorter analysis times and improved resolution are the result Gradient pump with integrated degassing at up to 600 bar Autosampler for 0 1 100 pL injection from 2 mL or 6 mL vials Column oven for stable temperatures up to 60 C Variable wavelength detector with 80 Hz data rate Gradient Configurations Isocratic Configuration Isocratic Pump Gradient Pump Gradient Pump Manual Injector Manual Injector Autosampler Column Oven Variable Variable Wavelength Variable Wavelength Wavelength Detector Detector Detector Choose from three standard configurations to fit your LC workflow then upgrade as required to meet your lab s future needs Manual injector An easy to use easy to maintain solution for low throughput sampling the manual injector includes a long life Rheodyne 2 position 6 port sample injection valve and standard 20 uL loop Front access to the valve facilitates easy sample injection and straightforward exchange of sample loops Autosampl
14. vey amp High resolution Scan The survey scan is the starting point of the TurboDDS process The tetra n butylammonium TBA ion was identified as the only target ion m z 242 3 to further analyze The tree report shows the high resolution MS spectrum in brown of the analyte confirming the single charge state for the target ion m z 242 3 MS2 Spectrum From the survey scan the precursor ion m z 242 3 is identified for MS MS MS analysis The MS spectrum in green includes several product ions the most abundant is m z 186 3 This is TBA minus one butyl side chain 56 0 The ion intensity values displayed below the peak m z values on the spectra indicate that Path A is the predominant breakdown pathway MSn Path A From the MS MS analysis the m z 186 3 ion is isolated and the MS analysis generates the product ion m z 130 2 This represents the loss of another butyl side chain group 56 0 The m z 130 2 ion is isolated and the MS analysis produces a product ion m z 74 2 representing the loss of the third butyl side chain group 56 0 An MS analysis is not performed because the scan of the MS product ions fails to meet the ion intensity threshold defined in the method CH CH 57 M 242 CH CH CH CH CH CH N en CH N 57 CH 6 CH N 100 CH P CH ET CH 3 Tetrabutylammonium bromide salt tetrabutyl ammoni um ion M t m z 242 Each side chai

Agilent 500 Ion Trap LCMS

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