Agilent 6460 Triple Quadrupole LC/MS System: Achieve Ultra-Sensitive Quantitation(1)(1)
Contents
1. Agilent Technologies AGILENT 6460 TRIPLE QUADRUPOLE LC MS SYSTEM SUPERIOR SENSITIVITY AND PRODUCTIVITY FOR YOUR CHALLENGES IN QUANTITATIVE ANALYSIS The Agilent 6460 Triple Quadrupole LC MS System is equipped with Agilent Jet Stream Technology to provide ultra sensitive performance for analytes in the most complex matrices This makes the 6460 the instrument of choice for a wide range of applications including environmental and food safety analysis clinical research and peptide quantitation The 6460 provides a fast and robust solution for simultaneously quantifying screening and confirming analytes using triggered MRM tMRM The Agilent 6460 Triple Quadrupole LC MS is engineered to deliver robust accurate quantitation of analytes present at extremely low levels Updated electronics enable high speed data acquisition while fast polarity switching makes the instrument an ideal choice for rapid separations and high throughput analyses of diverse compound types The 6460 Triple Quad LC MS is a feature packed new addition to the Agilent Triple Quadrupole product family e Excellent sensitivity for many applications e 1 ms dwell time with no collision cell cross talk e Very fast polarity switching for multi analyte studies Dynamic Multiple Reaction Monitoring dMRM to maximize MRM efficiency Triggered Multiple Reaction Monitoring tMRM for simultaneous quantitation and confirmation The 6460 Triple Quad offers full2. control of Agilent LC systems to provide an optimal solution for UHPLC standard LC or nanoLC chromatography Seamless support and a single point of service contact minimize delays and maximize your up time W mim Robust Ultra Trace Quantitation at UHPLC Flow Rates Meet the challenges of multiplexed quantitative analysis for a wide variety of compound classes The 6460 Triple Quadrupole with Agilent Jet Stream technology is built to deliver the highest level of robustness accuracy and reproducibility in complex sample matrices such as those commonly encountered in food safety and environmental analysis Ref Library Match Score 95 1 1 168 1 0 8 tMRM Product lon 0 6 4 77 0 Spectrum 0 4 105 0 0 2 290 1 Reference Library Spectrum 75 100 125 150 175 200 225 250 275 300 Mass to charge m z Figure 2 A library match score of 95 1 is based on a close correlation between the tMRM product ion spectrum and the reference library spectrum 0 5 1 0 1 5 2 0 2 5 3 0 3 5 4 0 4 5 5 0 5 5 6 0 Acquisition time min Figure 1 dAMRM analysis of 224 pesticides at the 500 parts per trillion level in less than 7 minutes Triggered MRM Data Dependent Scanning for Compound Confirmation Triggered MRM tMRM acquisition is available on all Agilent Triple Quadrupole LC MS systems tMRM acquisition effectively combines MRM quantitative analysis with data dependent acquisi tion of a product ion sp
3. ectrum which can also be used for library searching identification and confirmation tMRM analysis is faster and more sensitive than conventional product ion scan ning and enables quantitative and qualitative analysis in a single LC MS run In tMRM analysis mode when the primary transitions exceed a user defined threshold an additional set of secondary transitions are triggered The primary transi tions are used for quantitation and can be combined with the secondary triggered transitions to generate a tMRM product ion spectrum This product ion spectra can be searched against an application specitic library such as the Agilent Personal Compound Database and Library or against publicly available spectral libraries Advantages of tMRM e Confirmation of compound ID to avoid false positives e Faster and more sensitive than data dependent full scans e Optimal collision energy for each ion transition acquired Maximize Your Quantitative Performance Learn more with Dynamic MRM www agilent com chem 000 Dynamic MRM dMRM creates more powerful quantitative Buy online methods by grouping MRMs in retention time windows instead of www agilent com chem store time segments Compound specific MRMs and their retention times are easily imported into the dMRM method that can quantify up to Find a local Agilent customer center 4 000 compounds in a single run Fast MRM speeds support the www agilent com chem contactus analysis of over 100 overla
4. pping compounds in a given retention time window USA and Canada 1 800 227 9770 agilent_inquiries agilent com Europe info_agilent agilent com ioe CUBR RF asia Pci inquiry Ilsca agilent com For Research Use Only Not for use in diagnostic procedures This information is subject to change without notice Agilent Technologies Inc 2012 12345678 9 10111213141516 Printed in the USA February 3 2012 5990 9500EN Figure 3 Using dMRM a retention time window is profiled for each analyte and the list of analytes is adjusted dynamically based on chromatographic run time Analytes are only monitored when they are expected to elute improving the overall duty cycle Agilent The Agilent Value Promise 10 years of guaranteed FL performance WA In addition to our continually evolving products Agilent offers the industry s only 10 year value guarantee Agilent gurantees you at least 10 years of instrument use from your date of purchase or we will credit you with the residual value of that system toward an upgraded model It s our way of assuring you a safe purchase now and protecting your investment ot E Agilent Technologies
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Agilent 6460 Triple Quadrupole LC/MS System: Achieve Ultra Sensitive Quantitation(1)(1)