Agilent Improved Throughput of deoxyNucleosides Using New Column Configurations
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1. Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material Information descriptions and specifications in this publication are subject to change without notice Printed in the USA April 25 2002 5988 6328EN ie Agilent Technologies2. Improved Throughput of deoxyNucleosides Using New Column Configurations Application o o o z gt e Biochemical 2 Robert Ricker Analysis of deoxynucleosides is commonly performed on hydrolyzed DNA and other Highlights samples While LC allows quantitation LC MS allows position confirmation of standard deoxynucleosides modified deoxynucleosides and impurities The following is a very rapid method compatable with LC MS Agilent ZOH BAN SEGN plovides good selectivity for the 4 standard deoxynucleosides ZORBAX SB CN 5 um Shorter columns having 3 5 um G H mAU 4 6 x 150 mm particles can provide very rapid Agilent P N 883975 905 separations suitable for LC MS T A Smaller particle columns 3 5 vs 5 um can be used at increased flow rates without reduced loss in resolution 3 5 um 4 6 x 50 mm Agilent P N 835975 905 0 1 2 3 4 5 6 7 8 min Conditions LC HP1090 Mobile Phase A 0 1 TFA B 97 5 MeOH 2 5 H 0 0 1 TFA UV 254 nm Flow 1 0 mL min 30 C oft Agilent Technologies Robert Ricker is an application chemist based at Agilent Technologies Wilmington Delaware For more information on our products and services visit our website at www agilent com chem Copyright 2002 Agilent Technologies Inc All Rights Reserved Reproduction adaptation or translation without prior written permission is prohibited except as allowed under the copyright laws
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Agilent Improved Throughput of deoxyNucleosides Using New Column Configurations