Agilent High-Speed Separation of Pesticide Mixture Manual
Contents
1. Application Environmental Robert Ricker a High Speed Separation of Pesticide Mixture Columns with particle sizes under 5 um and columns with dimensions up to ten times smaller than standard analytical sized columns are ideal for high speed analyses In this application smaller particles and column dimensions are used to resolve six pesticides in less than two minutes Extra column volume is a crucial factor in chromatographic performance when using low volume columns In this example no modification to a modern instrument plumbing or flow cell is necessary Operating Conditions HPLC System Agilent 1100 with quaternary pump Column ZORBAX Eclipse XDB C18 Rapid Resolution 3 5m Cartridge Column 4 6 x 30 mm Agilent Part No 931975 932 Mobile Phase MeOH water 60 40 Detection UV 254 nm with standard flow cell 13 uL Flow 2 mL min Temperature ambient mAU ZORBAX Eclipse XDB C18 500 400 1 Tebuthiuron 2 Atrazine 300 3 Diuron 4 Propazine 200 5 Propranil 6 Prometryne 100 0 0 0 5 1 1 5 2 2 5 3 3 5 a gt Pe Highlights e Reducing column length and particle size simultaneously can Reduce analysis time Maintain resolution Reduce solvent use e Higher flow rates are possible due to decreased pressure when using ZORBAX Rapid Resolution cartridge columns min ie Agilent Technologies Robert Ricker is an application chemist based at Agilent Technologies Wilmington Delaw2. are For more information on our products and services visit our website at www agilent com chem Copyright 2002 Agilent Technologies Inc All Rights Reserved Reproduction adaptation or translation without prior written permission is prohibited except as allowed under the copyright laws Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material Information descriptions and specifications in this publication are subject to change without notice Printed in the USA April 25 2002 5988 6347EN ie Agilent Technologies
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