Agilent Analysis of Glyphosate in Water with Postcolumn Derivatization using HPLC Manual
Contents
1. Abstract The HPLC method presented here was used for the direct analysis of glyphosate in water with postcolumn derivatization HPLC method performance Limit of detection lppb Repeatability of RT over 10 runs lt 0 8 of areas over 10 runs lt 2 2 Norm Analysis of Glyphosate in Water with Postcolumn Derivatization using HPLC Rainer Schuster Food Column 150 4 mm cation exchange K form from Pickering 8 um Mobile phase A 5 mM KH PO pH 2 0 B 5mM KOH Flow rate 0 4 ml min Gradient at 15 min 0 B at 17 min 100 B Column compartment 55 C Injection vol 50 ul standard Fluorescence detector Excitation wavelength 230 nm or 330 nm Emission wavelength 425 nm Slit width excitation 2 mm 25 nm Slit width emission 1 4 mm 50 nm Slit width emission 2 4 mm 50 nm Photomultiplier gain 2 Cut off filter 370 nm Lamp 55 Hz always on Response time 4 s 25 5 T5 10 Time min Figure 1 EIC traces from amine standards 125 We 20 Derivatization reagent pump flow rate for hydrolization agent 0 3 ml min OCI flow rate for derivatization agent 0 3 ml min OPA Sample preparation None Agilent Technologies Innovating the HP Way 2 Agilent Technologies e S e Innovating the HP Way
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