Agilent Metanephrines in Urine Manual
Contents
1. Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material Information descriptions and specifications in this publication are subject to change without notice This publication was originally published in 2009 Agilent Technologies Inc 2011 Published in USA July 12 2011 SI 02160 7 Agilent Technologies2. Metanephrines in Urine o e e Application Note Bioanalysis Introduction An HPLC separation of four metanephrines in a urine sample was accomplished with an Agilent Polaris C18 A column cig Agilent Technologies Conditions Column Agilent Polaris C18 A 4 6 x 150 mm 5 um p n A2000150X046 Sample Standard and patient urine sample Sample concentration Urine standard normetanephrine 3543 nmol L metanephrine 1496 nmol L 3 methoxytyramine 1638 nmol L internal standard 4 O methyldopamine 0 1 M KH PO pH 6 0 20 mg L 1 octanesulfonic acid sodium salt 1 g L sodium EDTA 6 acetonitrile 1 0 mL min Injection volume 5ul 30 C ECD Antec Decade 0 63 V Glassy Carbon Ag AgCl Mobile phase Flow rate Temperature Detector Peak identification for both figures 1 Normetanephrine 2 Metanephrine 3 3 methoxytyramine 3 MT 4 4 0 methyldopamine IS 160 mV T2 Metanephrines in urine with an Agilent Polaris C18 A column and electrochemical detection on a standard urine sample Figure 1 RT min 0 1 ge eg 5 6 7 8 9 10 Figure 2 Metanephrines in urine with an Agilent Polaris C18 A column and electrochemical detection on a patient urine sample For More Information These data represent typical results For more information on our products and services visit our Web site at www agilent com chem www agilent com chem
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