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Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User Guide

1. A VOAs medium soil sample is handled quite differently from the water or low soil sample Typically the medium soil analysis is used if a 1 gram low soil analysis gave saturated peaks A nominal 4 0 gram sample of the soil is extracted with 9 0 mL of methanol and 1 0 mL of the system monitoring compounds 10 000 uL total methanol Of this 10 0 mL solution 100 uL is transferred into 4 9 mL of reagent water in a 5 mL syringe giving a total of 5 0 mL of sample to be purged As with water samples 10 uL of the internal standard solution is added prior to transferring the 5 0 mL in the syringe into the purging vessel The SOW specifies using Equation 9 to determine the concentration of an analyte in a medium soil sample A xIx V x 1000uL mL x Df Mosd oo 9 H848 A x RRF xV x W xD a where Ay Response of the analyte area I Amount of internal standard added ng V MeOH soil extract volume mL Df Dilution factor see definition in SOW Ais Response of the internal standard area RRF Relative response factor V3 Methanol aliquot volume purged uL W Wet weight of sample extracted with methanol g D Fractional solids 100 Moisture 100 SOW D 43 VOA Section 10 3 The 1000 uL mL term in the numerator is needed when V is expressed in mL 69 Calculations for VOAs Analysis Reference 70 The Df for medium soil samples is defined as 1 00 when exactly 10 mL of methanol is used to extract the soi
2. b Multiple forms all samples Select the check box in the upper left corner of the panel blank to generate one or more forms for all the samples in the sample list Select the forms from the left side of the screen 5 To print the form or forms selected in step 4 click the Print button Wait until all the related samples have been analyzed and the required input files generated from EnviroQuant Data Analysis before you attempt to print any environmental forms because each form requires information from several input files Although some forms might be correctly generated with only some of the input files present it is more likely that individual forms based on a partial set of input files would be incomplete or inaccurate For example an Organics Analysis Data Sheet Form 1 produced before the method blank has been processed would not be able to flag compounds that were detected in the blank Single form only To preview a form on the screen before printing select the form and sample as described in step 4 then click the Preview button The form appears on the screen The information is presented for display only you can t modify the data at this point You can view different parts of the form or print the form 57 58 Printing Forms Use the page arrow buttons at the lower left part of the window to view different pages of the form Next page piao gt ipil Previous page To print the form to the Windows d
3. Extract Vol 27 Extraction Type 27 Extraction volume 26 F Final Multiplier 26 Florisil cleanup 47 Form 9 52 Footer Version 57 Forms creating sample lists for 20 customizing labels on 57 generating 54 list of available 11 52 numbering pages on 58 preparing data for 14 previewing 55 printing 52 56 printing examples of 12 52 single form for single sample 55 Fraction type list of forms by 52 selecting 21 28 G GC Column 25 specifying ID for 25 GC data with two signals entering sample information for 32 modifying sample information for 33 GEL permeation chromatography 27 47 Global Page Number 58 GPC cleanup 27 47 101 Form 9 52 106 Header Information 20 23 27 displaying 35 filled in automatically for each sample 29 modifying 30 33 Heated Purge 26 73 Help accessing 13 HP Vectra 486 personal computer 8 Identification Summary Form 10 52 Initial calibration creating input file for 15 Form 6 52 response factors 45 sample list and 20 28 sample type 31 specifying results file for 30 Injection Volume 27 Input files for Enviroforms generating 14 Instrument ID 25 Internal standard abbreviating name of 44 calculating amount of 61 77 concentration levels 60 76 Form 8 52 specifying as compound type 42 44 ISTD compound type 42 44 J J flag 71 86 100 L Lab Code 25 57 Lab File Name 31 Lab File Path 31 Lab Name 2
4. How it works EnviroForms software manages and generates reports for lists of samples A list of samples may include blanks unknowns and initial and continuing calibration samples and the analytical results for each A sample list is associated with a list of compounds to report A compound list contains the names of all compounds and the QA QC parameters for reporting each compound 12 EnviroForms User s Guide Getting Started EnviroForms stores sample lists and compound lists in Environmental Reporting Methods ERMs Each ERM can contain up to three compound lists one each for volatiles semi volatiles and pesticides More than one sample list can be associated with each fraction The relationships between ERMs sample lists and compound lists are shown in Figure 1 Volatile Semivolatile Pesticide Compound List Compound List Compound List Figure 1 Environmental Reporting Method ERM Sample List 1 Sample List 1 Sample List 2 Sample List 3 Forms available EnviroForms User s Guide You can use EnviroForms software to print the forms in Table 1 for various fractions Table 1 Available forms Fraction Forms Volatiles Forms 1A 2A 2B 3A 3B 4A 5A 6A 7A 8A Semi volatiles Forms 1B 1C 2C 2D 3C 3D 4B 5B 6B 6C 7B 7C 8B 8C Pesticides Forms 1D 2E 2F 3E 3F 4C 6D 6E 6F 7D 8D 9 10 13 Getting Started 14 You can customize the final printed forms by specifying the
5. labels for some of the data that appears on the forms Without customization the terminology on the reports complies with the US Environmental Protection Agency s March 1993 Contract Laboratory Program Statement of Work USEPA CLP SOW With customization the forms can reflect the USEPA CLP SOW Version OLMO2 0 forms Example forms can be printed using the demo data supplied with your EnviroForms software load demo erm then select Print One of Each Form from the Help menu Related products Agilent EnviroForms software is optimized for laboratories that carry out GC or GC MS analyses with groups of 20 to 100 samples Use one of the following alternative products if your methodology is different or if you run less than 20 or more than 100 samples in a group e If your methodology requires reports for single samples only use the Custom Reports software that is part of EnviroQuant Mode of Data Analysis or Enhanced Mode of Data Analysis which is part of the MSD Productivity ChemStation software If your methodology includes long term studies using large sample groups or where historical records must be kept use a LIMS product such as Agilent s data systems e Ifyou are a participant in the US EPA Contract Laboratory Program use the reduced results rr files generated by Environmental Data Analysis to transfer the data to the application program you use to create the required diskette deliverables e Ifyou need further customi
6. Forms 1A and 1B Called N6 in EDA The highest calibration level used in instrument units Used to calculate the E flag exceeds calibration for Form 1 Table 8 lists the parameters for Surrogate compound types Table8 Surrogate types Type Description Amount The amount of surrogate compound added to the sample prior to sample preparation The units are the same as those used for quantitation The lower and upper bounds for the acceptable percentages of surrogate compound recovery for water and for soil Recoveries low high Table 9 lists the parameters for Matrix Spike compound types 47 Setting Up Compound Lists 48 Table9 Matrix spike types Type Description Amount Recoveries low high RPD The amount of matrix spike compound added to the sample prior to sample preparation The units are the same as those used for quantitation The lower and upper bounds for the acceptable percentages of matrix spike compound recovery for water and for soil The maximum acceptable value for relative percent deviation for each matrix type EnviroForms User s Guide Setting Up Compound Lists Additional fields for pesticides only all compound types Florisil Check GPC Check Performance Evaluation Resolution Ca EnviroForms User s Guide Check libration Nominal Amount Indicate with a check mark whether or not a Florisil check was performed If the check was performed
7. Production run 104 Ee Agilent Technologies 93 Calculations for Pesticide Analysis Calibration The calibration curve for pesticides PEST analysis is different from volatiles and semivolatiles in that external standard calibrations are used The calibration curve is constructed using the levels in Table 17 Table 17 Pesticide calibration Calibration level Analyte concentration Analyte on column ng mL pg per 1 pL injection LOW 5 10 20 50 5 10 20 50 MED 3x LOW 3 x LOW HIGH 30 x LOW 30 x LOW Reference SOW D 37 PEST Section 3 3 The calibration factors are computed using Equation 21 CF Ai ae 21 Ci Cy lt M noc where CF Calibration factor response per nanogram injected Ay Response of the analyte area or peak height Cinj Nanograms injected nanograms on column Cy Analyte concentration in standard ng mL pg uL Mnoc Ng on column Multiplier 0 001 Reference SOW D 48 PEST Section 9 2 2 94 EnviroForms User s Guide Reference EnviroForms User s Guide Calculations for Pesticide Analysis The term M 0 001 is needed if the standards are expressed in terms of ng mL in the standard solution A 1 0 uL injection of the 5 ng mL LOWstandard solution will deliver 5 picograms on column Since the CF must be expressed in terms of response per nanogram injected the conversion factor is needed to convert the 5 picograms injected to 0 005 nanograms injected If the pestici
8. Signal 2 1 to return to the sample list for the first signal 15 When all the changes have been made to the sample list click the Exit button You are prompted to confirm saving changes to the last sample you modified See Also To Modify a Sample List on page 35 To Display or Print Sample Information on page 37 EnviroForms User s Guide To Modify a Sample List EnviroForms User s Guide Setting Up Sample Lists 1 Ifthe sample list you want to edit is the one currently displayed in the title bar of the EnvForms window skip to step 2 Otherwise To load a different sample list in the same ERM click the Sample List button on the toolbar of the EnvForms program window To load a sample list in a different ERM select Open ERM from the File menu You are prompted to select a sample list Click the Edit Samples button on the toolbar of the EnvForms program window to display the Edit Sample Information dialog box 3 You can change the sample list in any of the following ways To change header information for any of the samples select a sample by clicking it in the list of samples You can then modify any of the fields on the panel by clicking the field then typing in a new value The sample information fields are described in To Set Default Header Information on page 25 and To Create a Sample List on page 30 earlier in this chapter You are prompted to save the changes when you select another sample to ed
9. a Compound List on page 41 Select Display Compound List from the Utilities menu A table of the compound list and the compound parameters appears on the screen Click the page arrow buttons at the lower left part of the window to view different pages of the table Next page af afPace f Previous page To print the list to the Windows default printer select Print from the File menu or click the Print button To exit the display window select Close from the File menu or click the Close button 51 Setting Up Compound Lists 52 EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide 5 Printing Forms Printing Forms 54 Example forms 54 Available formats 55 To Generate Environmental Forms 56 To Customize Labels Used on Forms 59 Ee Agilent Technologies 53 Printing Forms Printing Forms Once you have set up lists of samples and compounds as described in Chapter 3 and Chapter 4 you are ready to generate the environmental forms The following forms Table 13 are available depending on the fraction analyzed Table 13 Available forms Environmental form Volatiles Semi volatiles Pesticides 1 Organics Data Analysis Sheet 1A 1B 1C 1D 2 System Monitoring Compounds surrogates 2A 2B 2C 2D 2E 2F 3 Matrix Spike Matrix Spike Duplicate Recovery 3A 3B 3C 3D 3E 3F 4 Method Blank Summary Report 4A 4B 4C 5 Tune Evaluation MS data only
10. convert from ppb to ppm 59 Printing Forms Footer Version Change to OLM02 0 as shown in Figure 11 to customize forms for USEPA CLP SOW Version OLM02 0 Global Page Select this field to consecutively number the pages when you Number generate multiple forms as described in To Generate Environmental Forms on page 56 60 EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide Calculations for VOAs Analysis Calibration 62 Water Samples 64 Edit Volatile Sample Information panel 65 Low Soil 67 Medium Soil 69 VOA CROLs 71 Compound List Manager for Volatiles 72 Water 72 Low soil 72 Medium soil 73 CRQL calculations 73 J flags 73 Ng on column Multiplier 74 Further Concerns 75 Dilutions and surrogates 75 Heated purge 75 Calibrating soils 75 Production run 76 Apg Agilent Technologies 61 Calculations for VOAs Analysis Calibration The calibration curve for volatiles analysis is constructed using one of the following schemes Table 14 and Table 15 Table 14 Calibration using 5 0 mL samples Calibration level Analyte Analyte on column Int std concentration Int std on column concentration ng per 5 0 mL ug L ng mL ug L ng mL ug L ng mL ng per 5 0 mL 10 10 50 50 250 20 20 100 50 250 50 50 250 50 250 100 100 500 50 250 200 200 1000 50 250 Reference SOW D 22 VOA Section 5 5 1 Table 15 Calibration using 25 0 mL samples Calibration lev
11. enter the information described in Table 10 Table 10 Florisil check Entry Description Amount The amount of compound used for the Florisil check The units are the same as those used for quantitation Recoveries The lower and upper bounds for the percentages of low high compound recovered in the Florisil check Indicate with a check mark whether or not a GPC check was performed If the check was performed enter the information described in Table 11 Table 11 GPC check Entry Description Amount The amount of compound used for the GPC check The units are the same as those used for quantitation Recoveries The lower and upper bounds for the percentages of low high compound recovered in the GPC check Indicate with a check mark whether or not a performance evaluation was performed If the evaluation was performed enter the amount of compound used Indicate with a check mark whether or not a resolution check was performed The concentration of a compound in the calibration check standard Form 7E 49 Setting Up Compound Lists Table 12 Parameter correspondence EDA Edit compound page 2 Use Table 12 as a quick reminder of the corresponding compound reporting parameters between Environmental Data Analysis EDA and EnviroForms The compound information from the Edit Compound Page 2 dialog box of the Environmental Data Analysis program EDA is copied into the compound list in Enviro
12. factors for 45 specifying reporting parameters for 41 48 specifying types of 42 44 Continuing calibration creating input file for 15 Form 7 52 response factors 45 sample type 31 specifying results file for 30 Continuous liquid liquid extraction 27 Contract 25 57 Contract Lab information 25 Converting concentration units 25 57 Correction factor 25 Create Sample list dialog box 28 CROLs for pesticides 98 100 for semivolatiles 83 86 for volatiles 69 71 csv files 15 30 Customize Formset dialog box 57 D Date Due 24 Date Extracted 25 Date Received 24 Decanted 26 Detection limit 45 Dilution Factor 25 73 89 97 102 Dilution sample type 31 E Edit Default Sample Information dialog box 23 Edit Sample Information dialog box 29 Computer hardware requirements 8 63 87 Chemical Abstracts Service Number Concentration EnviroForms software 10 specifying 43 low soil 24 Environmental Data Analysis Cleanup medium soil 24 compound reporting parameters in 48 GPC 27 of unknown analyte 61 65 68 80 creating input files for EnviroForms 14 sulfur 27 81 94 96 cmpdlist csv file 38 39 EnviroForms User s Guide 105 Index Environmental Reporting Method ERM compound lists and 38 creating 21 description of 11 initial multipoint calibration 74 89 102 loading 33 35 54 samples lists and 20 EPA Sample Number 57 Example forms printing 12 52
13. fio B Samples Signal 2 New Sample Update Results Delete 2 Type in the information that you expect to be the same for most samples in the sample list including any of the fields described on the following pages When you have specified the desired default values click the Exit button 25 Setting Up Sample Lists Sample ID Type information Date Received The date the samples were received by the lab Date Due The date the report is due or the data when data acquisition must be completed Sample Type One of the sample types from Table 3 Table3 Sample types Sample Description Sample An unknown sample to be analyzed for target compounds Re Extraction A sample that was re extracted and reanalyzed Dilution A sample analyzed at a secondary dilution Matrix Spike A sample that contains known amounts of target compounds to check method reproducibility and matrix interference Matrix Spike Duplicate A duplicate of the Matrix Spike sample Method Blank A blank sample used to check for system contamination Initial Calibration A sample used to generate the initial calibration Initial Continuing Calibration The mid level initial calibration sample that the continuing calibration will be checked against Continuing Calibration The continuing calibration standard that is run and checked against the Init Cont Calib Std sample Tune Evaluation MS data only The sample run to check the BFB or D
14. for Pesticide Analysis where Cont Concentration from the quant report ng mL pg uL Moc Ng on column Multiplier 0 001 V Concentrated extract volume 10 000 uL Df Dilution Factor in Sample Information panel see definition in SOW V Volume of concentrated extract injected uL W Wet weight of sample extracted 30 g D Fractional solids 100 Moisture 100 1000 Converts 5 0 ng mL to 0 005 ng uL The nanogram on column term Mpg 0 001 is needed if a quantitation report shows the LOW standard at 5 0 ng mL If the quantitation report shows the LOW standard at 0 005 ng uL the nanogram on column term is set to 1 Percent moisture is the value entered in the Moisture field in the Sample Information panel for the sample 99 Calculations for Pesticide Analysis Pesticide CROLs 100 Single component analytes Reference The CRQLs that the SOW specifies for the single component pesticides are based on detection limits of 5 to 50 picograms on column 0 005 to 0 050 nanograms on column The SOW gives a table that converts the 5 10 or 50 picograms on column detection limit to the nominal CRQL concentration of the analyte in the original sample This is 0 05 0 10 or 0 50 ug L for water and 5 10 or 50 ug Kg for soil samples SOW C 8 Multi component analytes Reference The CRQLs that the SOW specifies for the multi component pesticides are based on detection limits of 100 200 or 500 pic
15. for that Cym that gives 333 mg Kg Set all terms to their nominal value for a low soil preparation Myo 2 uL V 1 000 uL V 2 uL W 30 g and D 1 and solve for Cyn A Cont 10 ug L gives correct CRQL of 333 33 ug Kg in a low soil sample When rounded off this is reported as 330 ug Kg When the calibration is set up to report standards at 10 25 40 60 and 80 ng uL Moc must be set to 2 and Report Detection Limit on column DLhoc must be set to 20 or 100 Thus the Cant term used when no analyte is found is the value of DL oc Mnoo When the calibration is set up to report standards at 20 50 80 120 and 160 nanograms on column M must be set to 1 and Report Detection Limit on column DL must be set to 10 or 50 Then the Cynt term used when no analyte is found is again the value of Dlige Mnoc Given that the medium soil CRQL is 10 000 ug Kg in the original sample Equation 19 can be solved for that Cyn that gives 10 000 ug Kg Set all terms to their nominal value for a medium soil preparation Moc 2 pL V 1 000 uL V 2 uL W 1 g and D 1 and solve for Cont A Con 10 ug L gives the correct CRQL of 10 000 ug Kg in a medium soil sample When the calibration is set up to report standards at 10 25 40 60 and 80 ng uL Moc must be set to 2 and Report Detection Limit on column must be set to 20 or 100 Thus the Cym term used when no analyte is found is the value of DLhoc Mnogo When the
16. information Semivolatiles 28 Sample Preparation information Pesticides 29 To Create a Sample List 30 EnviroForms User s Guide To Modify a Sample List 35 To Display or Print Sample Information 37 4 Setting Up Compound Lists Setting Up Compound Lists 40 To Load or Select a Compound List 41 To Modify a Compound List 43 Compound reporting parameters 45 Additional fields for pesticides only all compound types 49 To Display or Print a Compound List 51 5 Printing Forms Printing Forms 54 Example forms 54 Available formats 55 To Generate Environmental Forms 56 To Customize Labels Used on Forms 59 6 Calculations for VOAs Analysis Calibration 62 Water Samples 64 Edit Volatile Sample Information panel 65 Low Soil 67 Medium Soil 69 VOA CRQLs 71 Compound List Manager for Volatiles 72 Water 72 Lowsoil 72 Medium soil 73 EnviroForms User s Guide CRQL calculations 73 Jflags 73 Ng on column Multiplier Further Concerns 75 Dilutions and surrogates Heated purge 75 Calibrating soils 75 Production run 76 7 Calculations for SVs Analysis Calibration 78 Water Samples 81 Soil Samples 83 SVCROLs_ 85 Water 85 Low soil 86 Medium soil 86 CRQL calculations 87 J flags 88 Other Factors 89 Ng on column Multiplier GPC Cleanup Y N 89 Further Concerns 91 Dilutions and surrogates Calibrating soils 91 Production run 91 8 Calculations for Pesticide Analysis Calibration 94 Water Samples 96 EnviroForm
17. instruments with each instrument can have one of four Data Analysis modes Enhanced Quantitation EnviroQuant Aromatics in Gasoline and Drug Analysis EnviroQuant EPA mode is specifically designed to meet the needs of laboratories performing analyses compliant with USEPA methodology Configure a designated instrument number for EnviroQuant EPA mode and then click on the desktop ICON to run either the EnviroQuant Data Acquisition or the EnviroQuant Data Analysis mofe of operation 1 Ifthe Environmental Data Analysis program is not already running start it by double clicking the EDA icon If the Forms menu items are not already enabled select EDA Configuration from the File menu click the Forms Menus option then click OK 2 Establish a quantitation database entry for each target compound and update the calibration table as described in the Environmental Data Analysis User s Guide When the initial calibration is acceptable select Response Factors to Forms File from the InitCal menu to produce a cal csv file for each data file in the initial calibration table note cal csv file is saved under the data file EnviroForms User s Guide Getting Started Select Evaluate Data file as Continuing Cal to Forms File from the ConCal menu to generate a cal csv file for each continuing calibration sample 3 Select Make Compound List for Forms from the InitCal menu to generate a cmpdlist csv file Once the list is created you can
18. limits will be 5 10 50 100 200 or 500 If the LOW standard is reporting analytes at 0 005 ng uL the term Mnoc 1 and you must then enter the on column detection limits as 0 005 0 010 0 050 0 100 0 200 or 0 500 EnviroForms User s Guide Other Factors Calculations for Pesticide Analysis EnviroForms software uses some other factors in its calculations that affect the numbers reported on the Forms Ng on column Multiplier This is set on the Edit Sample Information screen and is used to convert ng mL to ng uL as needed in Equation 24 and Equation 26 SetM to 0 001 if the LOW standard is labeled as 5 0 ng mL or to 1 000 if the LOW standard is labeled as 0 005 ng mL GPC Cleanup Y N EnviroForms User s Guide This appears on the Edit Sample Information screen The value Y or N appears on Form 1 If Y appears on Form 1 the value reported is multiplied by 2 The SOW states that you can concentrate a GPC extract to 5 0 mL rather than 10 mL to account for the 5 0 mL lost in the GPC cleanup If you do this you must be sure to set V to 5 000 uL for each sample 103 Calculations for Pesticide Analysis Further Concerns Dilutions and surrogates The equations are set up to apply the Dilution Factor set in the Sample Information panel to all concentrations This works out correctly if the surrogates are added to the original sample and the sample is subsequently diluted and reanalyzed If samples are prescreened
19. the actual dry weight of the soil the dilution factor and the methanol volumes used when the sample is treated as a medium soil sample The J flag will be assigned whenever Cant is lt Dlng Mnoc 73 Calculations for VOAs Analysis 74 Ng on column Multiplier This field appears on the Edit Sample Information screen and is used to convert ng mL to ng uL as needed in Equation 6 Equation 8 and Equation 10 Set Myo to 5 if the LOW standard is reporting ug L Set Myo to 1 if the LOW standard is reporting nanograms on column EnviroForms User s Guide Further Concerns Calculations for VOAs Analysis Dilutions and surrogates Heated purge The equations are set up to apply the Dilution Factor set in the Sample Information panel to all concentrations This works out correctly if the surrogates are added to the original sample and the sample is subsequently diluted and reanalyzed If samples are prescreened to determine if a dilution is needed there can be problems with the calculated values for the surrogates concentration For example prescreening indicates that the sample needs a 1 5 dilution The analyst dilutes the sample adds the surrogates to the diluted sample and proceeds with the extraction or purging A dilution factor of 5 is entered into the Sample Information panel All analyte values will be corrected to the concentration in the original sample but all surrogate concentrations will be five times too
20. the surrogate exhibits a 100 recovery the value will be above the high point of the calibration The surrogate recovery is expected to be less than 100 so this should not present a problem 78 EnviroForms User s Guide Reference EnviroForms User s Guide Calculations for SVs Analysis The Pesticide Arochlor analytes can be included at a concentration of less than 10 ng uL in the continuing calibration standard 25 ng L to facilitate confirmations of these analytes If the Pesticide Arochlor analytes are in the continuing calibration mix they are not reported on Form 7 but instead are included in any quantitation reports for the continuing calibration standard The relative response factors RRF are computed using Equation 12 The SOW is quite emphatic in stressing that the concentration terms are expressed in concentration not amount on column however the RRF is the same number if the concentrations are expressed in either concentration ng L or nanograms on column RRF ee 12 is X where Ax Response of the analyte area Cis Concentration of the internal standard ng pL Ais Response of the internal standard area Cy Concentration of analyte ng pL SOW D 43 SV Section 5 4 Equation 1 in Chapter 6 RRFs for VOAs expresses the concentration in ng mL mg L in the original sample Equation 12 for Semivolatiles expresses the concentration in terms of ng mL in the concentrated 1 0 mL extract which in thi
21. to determine if a dilution is needed there can be problems with the calculated values for the surrogates concentration For example prescreening indicates that the sample needs a 1 5 dilution The analyst dilutes the sample adds the surrogates to the diluted sample and proceeds with the extraction or purging A dilution factor of 5 is entered into the Sample Information panel All analyte values will be corrected to the concentration in the original samples but all surrogate concentrations will be five times too high The best workaround for this is to set a quant dilution factor of 0 2 and apply it to only the surrogates Then the number in the quant report will be one fifth of what it should be The dilution factor used in EnviroForms software will then correct this to the proper concentration Small dilution factors can give errors because the formats used in CSV MAC to write out QUANT CSV are limited to two decimal places The formats in CSV MAC may need to be changed to overcome this Calibrating soils Production run 104 The calibrations for a soil are always Sample Type Calibration Matrix Level Water and Percent Moisture 100 A production run consists of all injections into a given system while the multipoint curve is valid An ERM can continue as long as the initial multipoint curve is valid You do not need to create a new ERM for each days work EnviroForms User s Guide Index A Al field from EDA 44 A
22. 0 ng mL or to 1 000 if the LOW standard is labeled as 0 005 ng mL GPC Cleanup Y N This appears only on the Edit Semivolatiles Sample Information screen The value Y or N appears on Form 1 If Y appears on Form 1 the value reported by Equation 17 and Equation 19 is EnviroForms User s Guide 89 Calculations for SVs Analysis 90 Reference multiplied by 2 The SOW states that you can concentrate a GPC extract to 500 uL rather than 1000 uL to account for the 5 0 mL lost in the GPC cleanup If you do this you must be sure to set V to 500 uL for each sample SOW D 30 SV Section 3 6 1 EnviroForms User s Guide Further Concerns Calculations for SVs Analysis Dilutions and surrogates The equations are set up to apply the Dilution Factor set in the Sample Information panel to all concentrations This works out correctly if the surrogates are added to the original sample and the sample is subsequently diluted and reanalyzed If samples are prescreened to determine if a dilution is needed there can be problems with the calculated values for the surrogate concentrations For example prescreening indicates that the sample needs a 1 5 dilution The analyst dilutes the sample adds the surrogates to the diluted sample and proceeds with the extraction or purging A dilution factor of 5 is entered into the Sample Information panel All analyte values will be corrected to the concentration in the original sample but all surrog
23. 5 57 Lab Sample ID 31 Labels customizing 57 Load Compound List dialog box 39 M Matrix compound type 42 44 specifying 24 Matrix spike amount and acceptable recovery range 46 Form 3 52 recovery amount and acceptable range 46 sample type 31 Maximum field in EDA 45 Maximum Response Factor Dev 45 Maximum RSD 45 Methanol extraction volume 26 Method Blank sample type 31 Summary Report 52 Method Name 43 Microsoft Windows 8 Minimum Response Factor 45 Mix A and B 45 Moisture percent 26 27 MS DOS 8 Multiple forms printing 55 Multiplier 26 61 78 92 97 N4 field in EDA 45 N6 field in EDA 45 Ng on column Multiplier 26 61 72 78 87 92 97 101 0 Online help accessing 13 Organics Data Analysis Sheet Form 1 52 P Percent moisture 26 27 97 Performance requirements for optimum 8 Performance Evaluation 47 EnviroForms User s Guide Pesticides analyzing soil for 96 analyzing water for 94 95 calculations used for 92 102 Identification Suymmary Form10 52 list of forms for 11 52 selecting as fraction type 21 28 specifying cleanup techniques for 47 specifying compound Mix A or B for 45 specifying sample prep information for 27 pH specifying 27 ppb converting from 26 57 ppm converting to 26 57 Previewing forms 55 Preview Print dialog box Multiple Forms option on 55 Preview Print Forms dialog box 54 Printing compound information 43 49 env
24. 5A BFB 5B DFTPP 1D 6 Initial Calibration Data 6A 6B 6C 6D 6E 6F 7 Continuing Calibration Check 7A 7B 7C 7D 8 Internal Standard Area and Retention Time Summary Report 8A 8B 8C 8D 9 Pesticide Florisil Check and GPC calibration 9 10 Pesticide Identification Summary 10 Single and MultiComponent Analytes Example forms Example forms can be printed by selecting Print One of Each Form from the Help menu You can use data in an ERM you have created or use the demo data supplied with your EnviroForms software load demo erm 54 EnviroForms User s Guide Printing Forms Available formats EnviroForms User s Guide You can customize the final printed forms by specifying the labels for some of the data that appears on the forms See To Customize Labels Used on Forms on page 59 in this chapter Without customization the terminology on the reports complies with the US Environmental Protection Agency s March 1993 Contract Laboratory Program Statement of Work USEPA CLP SOW 55 Printing Forms To Generate Environmental Forms 56 1 Ifthe sample list you want to generate forms for is the one currently displayed in the title bar of the EnvForms window skip to step 2 Otherwise To load a different sample list in the same ERM click the Sample List button on the toolbar of the EnvForms program window To load a sample list in a different ERM select Open ERM from the File menu You are prompted to select a sample li
25. 86 CRQL calculations 87 J flags 88 Other Factors 89 Ng on column Multiplier 89 GPC Cleanup Y N 89 Further Concerns 91 Dilutions and surrogates 91 Calibrating soils 91 Production run 91 Apg Agilent Technologies 77 Calculations for SVs Analysis Calibration The calibration curve for semivolatiles SVs analysis is constructed using 2 0 uL injections of solutions at the following concentrations 1 0 ug mL 1 0 ng mL See Table 16 Table 16 SV calibration Calibration level Analyte concentration Analyte injected Int std concentration Int std injected pg mL pg pLl ng per 2 pL ng pLl ng per 2 pL 10 10 20 20 40 25 25 50 20 40 40 40 80 20 40 60 60 120 20 40 80 80 160 20 40 Reference SOW D 38 SV Section 3 2 It is not totally clear if the SOW prefers calibrations be set up using the calibration level and response or if they are to be set up using nanograms on column and response In the first case the continuing calibration will give quant reports with concentrations of 25 In the second case continuing calibrations will give quant reports with concentrations of 50 It is recommended that the SVs analysis be set up reporting nanograms on column Nine of the analytes will not be present in the 10 ppm standard due to the difficulty of detection of these analytes when less than 50 nanograms is injected on the GC column Each sample will have 100 ug of the surrogates added at the beginning of the analysis If
26. Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide 2 Agilent Technologies Notices Agilent Technologies Inc 2003 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G1701 90052 Edition Fourth edition August 2003 Printed in USA Agilent Technologies Inc 2850 Centerville Road Wilmington DE 19808 1610 USA Acknowledgements Microsoft Windows 2000 and Win dows XP are U S registered trademarks of Microsoft Corporation Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written
27. FTPP tuning Matrix Water Soil Low or Soil Medium Reference The reference blank to associate with this sample Click the Blank arrow to the right of this field to view a list of blanks The blanks are listed by Sample Names and Lab File Names 26 EnviroForms User s Guide Setting Up Sample Lists Contract Lab information Lab Name Lab Code Contract Case No SAS No SDG No Instrument ID GC Column GC Column ID The name of your laboratory The name or number of the project for the samples For CLP forms use your lab code assigned by the EPA The contract number or code For EPA work the contract number is included with the sample when it arrives at your lab The designation for the site where the sample was collected For CLP forms enter the case number that was included with the sample when it arrived at your lab The designation for the location where the sample was collected at the site For CLP forms enter the SAS number that was included with the sample when it arrived at your lab The name of the current sample list or sample delivery group For CLP forms enter the sample delivery group SDG number that was included with the sample when it arrived at your lab The identification of the instrument used to analyze the samples This designation must be unique within your laboratory This value will be overwritten for individual samples if their quant csv file identifies the instrument The
28. Forms when you load a compound list from a Data Analysis method as described in To Load or Select a Compound List on page 41 EnviroForms For compound types Compound list manager Signal level Minimum Maximum N4 N6 Calibration Range Upper Limit Surrogate amount Matrix A conc LOW Matrix A conc HIGH Matrix B conc LOW Matrix B conc HIGH Al Matrix spike amount Matrix A conc LOW Matrix A conc HIGH Matrix A conc Dev Matrix B conc LOW Matrix B conc HIGH Matrix B conc Dev Minimum Response Factor Target Matrix Surr ISTD Maximum RSD Target Matrix Surr ISTD Max Response Factor Dev Target Matrix Surr ISTD Report Detection Limit on column Target Matrix Surr ISTD Surrogate Amount Surrogate only Surrogate Water Low Surrogate only Surrogate Water High Surrogate only Surrogate Soil Low Surrogate only Surrogate Soil High Surrogate only Abbrev Surrogate only Matrix Spike Amount Matrix spike only Matrix Water Low Matrix spike only Matrix Water High Matrix spike only Matrix Water RPD Matrix spike only Matrix Soil Low Matrix spike only Matrix Soil High Matrix spike only Matrix Soil RPD Matrix spike only 50 EnviroForms User s Guide Setting Up Compound Lists To Display or Print a Compound List EnviroForms User s Guide If the compound list you wish to view is already loaded skip to step 2 Otherwise load the compound list of interest as described in To Load or Select
29. O WOAD Case No siz Date Received fi 25791 SAS No Date Due 25 91 SDG No fyswoot Sample Type Method Blank B Instrument ID HP5 Matrix Wwe E GC Column esms Reference Blank VBLKOT E GC Column ID fo20 Sample Preparation Date Extracted fi ABS Sample wt vol jo Units g or ml Mc MeDH Soil Extract Vol Moisture not decanted fico MeDH Aliquot Volume Heated Purge Y N E Dilution Factor Ng on column Multiplier Final Muftiplier Figure 12 Sample Information The actual volume purged Vha is the value set in the Sample wt vol field of the Sample Information panel for the individual sample Since the matrix in this example is water MeOH Soil Extract Vol and MeOH Aliquot Volume are not used The equation used to calculate the concentration of a volatiles water sample is derived from Equation 4 by substituting C X Mnoc for to give Equation 5 65 Calculations for VOAs Analysis 5 Further substitution of Cyn for the elements of Equation 2 on the right hand side of Equation 5 gives Equation 6 the equation used by EnviroForms software to calculate the concentration of a VOAs water sample Cant Mnoc Pf nt ug L 4 6 Vo where Cont Concentration from the quant report ug L ng mL Mnoc Ng on column Multiplier 5 or 25 mL Df Dilution factor Vo Volume actually purged from the Sample Information pane 5 or 25 mL 66 EnviroForms User s Guide Low Soil Reference EnviroForm
30. agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met EnviroForms User s Guide Contents 1 Introduction EnviroForms Software 8 What youneed 8 2 Getting Started What is Agilent EnviroForms Software 12 How it works 12 Forms available 13 Related products 14 Online help 15 To Prepare Data for Forms Production 16 Input files required for forms 16 To Produce Environmental Forms 19 3 Setting Up Sample Lists Setting Up Sample Lists 22 To Create a New ERM 23 To Set Default Header Information 25 Sample ID Type information 26 Contract Lab information 27 Sample Preparation information All fractions 27 Sample Preparation information Volatiles 28 Sample Preparation
31. are chosen to have chemical properties similar to those of the target compounds Surrogates are compounds not normally found in environmental media 46 EnviroForms User s Guide Mix A Mix B Minimum Response Factor Maximum RSD Maximum Response Factor Dev Report Detection Limit on column Calibration Range Upper Limit Surrogate Matrix Spike EnviroForms User s Guide Setting Up Compound Lists Pesticides only Used to specify compounds for Form 7E Select this field if the compound is present in calibration mix A Pesticides only Used to specify compounds for Form 7E Select this field if the compound is present in calibration mix B The minimum acceptable response factor for the compound Values below this minimum are flagged on Forms 6A and 6B initial calibration and Forms 7A and 7B continuing calibration Called Signal level minimum in EDA The maximum acceptable standard deviation of the response factors in the initial calibration Values above this maximum are flagged on Forms 6A and 6B initial calibration Called Maximum in EDA The maximum percentage deviation of the response factor that is acceptable in the daily or continuing calibration Values above this maximum are flagged on Forms 7A and 7B continuing calibration Called N4 in EDA The minimum detection limit for the compound in soil and water Compounds detected at a concentration below this value or not at all are flagged on
32. ate concentrations will be five times too high The best workaround for this is to set a Quant dilution factor of 0 2 and apply it to only the surrogates Then the number in the quant report will be one fifth of what it should be The dilution factor used in EnviroForms software will then correct this to the proper concentration Small dilution factors can give errors because the formats used in CSV MAC to write out QUANT CSV are limited to two decimal places The formats in CSV MAC may need to be changed to overcome this Calibrating soils Production run EnviroForms User s Guide The calibrations for a soil are always Sample Type Calibration Matrix Level Water and Percent Moisture 100 A production run consists of all injections into a given system while the multipoint curve is valid An ERM can continue as long as the initial multipoint curve is valid You do not need to create a new ERM for each days work 91 Calculations for SVs Analysis 92 EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide Calculations for Pesticide Analysis Calibration 94 Water Samples 96 Soil Samples 98 Pesticide CROLs 100 Single component analytes 100 Multi component analytes 100 Water 101 Soil 101 CRQOL calculations 101 Jflags 102 Other Factors 103 Ng on column Multiplier 103 GPC Cleanup Y N 103 Further Concerns 104 Dilutions and surrogates 104 Calibrating soils 104
33. bbreviations for compound names 44 Agilent EnviroForms software computer requirements for 8 introduction to 8 10 Agilent G1032C EnviroQuant software 8 Agilent G1034C MS ChemStation software 8 Aliquot volume 26 C cal csv file 30 Calculations used for pesticides 91 102 used for semi volatiles 75 89 used for volatiles 59 74 Calibration continuing 15 curve 60 76 92 E flag on Form 1 45 flags on Forms 6A and 6B 45 Forms 6 and 7 52 initial 15 of soil 73 89 102 sample types 31 specifying nominal amount 47 specifying the range upper limit 45 Calibration factors calculating 92 Calibration Nominal Amount 47 Calibration Range Upper Limit 45 CAS Number 43 Case Number 25 57 Compound list adding compounds to 42 converting units for Form 7 57 creating input file for 15 definition of 10 38 deleting compounds from 42 empty innew ERM 21 28 modifying 41 printing 43 49 renaming compounds in 43 reordering 42 viewing 49 Compound List Manager dialog box 41 70 description of parameters on 43 Compound Type 42 44 Compounds adding to compound list 42 calculating concentration of 61 65 68 80 81 94 96 concentration levels 60 76 92 deleting from compound list 42 determining list for reporting 38 flagged on forms when not detected 45 modifying information for 41 naming 43 renaming 43 reordering 42 specifying minimum response
34. calibration is set up to report standards at 20 50 80 120 and 160 nanograms on column M must be set to 1 and Report Detection Limit on column DL must be set to 10 or 50 Then the Cn term used when no analyte is found is again the value of Dlyg o Mnoc EnviroForms User s Guide CROL calculations EnviroForms User s Guide Calculations for SVs Analysis If the analyte is not found EnviroForms software will calculate a Corgi using Equation 20 and substitute that for Com into Equation 17 or Equation 19 as appropriate where DL Coa noc 20 M noc Cont Value of Com to give the CROL concentration If standards are reported as 10 25 40 60 and 80 DLaoc Report Detection Limit on column 20 or 100 in the Compound List M age Ng on column Multiplier 1 or 2 uL in the Sample List If standards are reported as 20 50 80 120 and 160 Dl noc Report Detection Limit on column 10 or 50 in the Compound List Mnoc Ng on column Multiplier uL in the Sample List The value for Report Detection Limit in the Compound List can be treated as an information only field The important field is the Ng on column Multiplier field in the Sample List You must adjust the Report Detection Limit on column numbers DL 9 to match how the standards are reported Each sample can give a different CRQL The CRQL is dependent on the actual sample size the actual dry weight of the soil the final extract volumes
35. ctor for 25 entering ID Type information for 24 GPC or sulfur cleanup 27 header information for 20 23 27 loading quantitative results 30 naming 31 reference blanks for 24 specifying moisture in 26 27 specifying Contract Lab information for 25 specifying extraction type for 27 specifying matrix type for 24 specifying pH of 27 specifying preparation information for 25 specifying type of 24 31 SAS Number 25 57 SDG Number 25 57 Semivolatiles analyzing soil for 81 analyzing water for 79 80 calculations used for 76 89 list of forms for 11 52 selecting as fraction type 21 28 specifying sample prep information for 26 Separatory funnel extraction 27 Setting up compound lists 38 Setting up sample lists 20 Signal 2 button 32 33 Signal level minimum field in EDA 45 Soil aliquot volume 26 calibrating 73 89 102 extraction volume 26 matrix type 24 pesticide analysis in 96 semivolatile analysis in 81 volatile analysis in 65 68 Sonication 27 Special Analytical Services SAS number 25 Sulfur Cleanup 27 Surr compound type 42 44 107 Index Surrogate 73 89 102 abbreviating name of 44 specifying amount and recovery range 45 System Monitoring Compounds Form 2 52 T Target compound type 42 44 To create anew ERM 21 To create a sample list 28 To customize labels used on forms 57 To display or print a compound list 49 To display or print sample informat
36. d and reanalyzed Dilution A sample analyzed at a secondary dilution Matrix Spike A sample that contains known amounts of target compounds to check method reproducibility and matrix interference Matrix Spike Duplicate A duplicate of the Matrix Spike sample Method Blank A blank sample used to check for system contamination Initial Calibration A sample used to generate the initial calibration Initial Continuing Calibration The mid level initial calibration sample that the continuing calibration will be checked against EnviroForms User s Guide 33 Setting Up Sample Lists 34 Table5 Sample types continued Sample type Description Continuing Calibration The continuing calibration standard that is run and checked against the Init Cont Calib Std sample Tune Evaluation MS data only The sample run to check the BFB or DFTPP tuning 13 Repeat step 8 through step 12 for each sample you wish to include in this sample list As you move to the next sample you are prompted to confirm that you want to save changes to the current sample 14 GC data with two signals only To modify sample information for a different signal click the Signal 1 2 button The sample information for signal 2 will be displayed You can modify the Lab File Path the Contract Lab information and the Sample Preparation information You cannot select a quant file add a new sample or delete a sample Use the same button now labeled
37. de standards are expressed in terms of ng mL or ng uL i e the LOW standard is labeled 0 005 ng uL instead of 5 pg uL the conversion factor is set to 1 0 The SOW states that manual injections must be 2 0 uL If autosamplers are used injections can be 1 0 uL The same volume must be used for all standards blanks and samples SOW D 38 PEST Section 4 1 Equation 21 can be recast to give Equation 22 A C xM 22 95 Calculations for Pesticide Analysis Water Samples The SOW specifies using Equation 23 to determine the concentration of an analyte in a pesticide water sample ug L ae 23 where Ay Response of the analyte area V Concentrated extract volume 10 000 uL Df Dilution factor see definition in SOW CF Calibration factor for the MED level area per ng injected Vo Original sample volume extracted 1 000 mL V Volume of concentrated extract injected uL Reference SOW D 52 SV Section 13 5 1 The equation used to calculate the concentration of an analyte in a pesticide water sample is derived from Equation 23 by first substituting Cis X Mhogo for A CF to give Equation 24 C xM xV x Df ug L qnt noc t 24 Vo X V where Cont Concentration from the quant report ng mL pg uL Mnoc Ng on column Multiplier 0 001 V Concentrated extract volume 10 000 uL Df Dilution factor from Sample Information panel see definition in SOW 96 EnviroForms User s Guide Env
38. e type of csv file appropriate to the sample type Table 4 Table4 csv file usage File type Description Use cal csv For initial or continuing calibration samples Use quant csv For samples blanks and matrix spikes Use tune csv MS data only For the tune evaluation sample 11 Click the csv file in the list then click OK As with any Windows file selection box you can also load the file by double clicking the file name of interest 12 If necessary modify any of the default header information for the current sample by clicking the field of interest and typing EnviroForms User s Guide Setting Up Sample Lists in the desired values The following parameters usually have to be modified for each sample Sample Name Unique name to identify the individual sample Lab Sample ID The name this sample is known by within your lab up to eight characters Lab FileName The name of the data file created when the sample was analyzed including the d extension You can leave this field blank if the sample analysis is not yet complete Lab File Path The full path name for the directory where the data file is located For example data files acquired on an MS ChemStation are often stored in c msdchem 1 data Sample Type Select a type from Table 5 for this sample Table5 Sample types Sample type Description Sample An unknown sample to be analyzed for target compounds Re Extraction A sample that was re extracte
39. earest percent Don t type in the sign Decanted Select this box if the samples were decanted Leave this box Y N blank if the samples were not decanted pH The pH of the samples to one decimal place accuracy Extraction Type SepF for separatory funnel Cont for continuous liquid liquid or Sonc for sonication GPC Cleanup Select this box if the samples were cleaned up with GPC Leave Y N this box blank if the samples were not cleaned up with GPC Sulfur Cleanup Select this box if the samples were cleaned up with sulfur Leave this box blank if the samples were not cleaned up with sulfur Concentrated The volume of the concentrated extract in microliters Extract Vol Injection The injection volume in microliters Volume EnviroForms User s Guide 29 Setting Up Sample Lists To Create a Sample List 30 Use the following procedure to create a new sample list for the current ERM shown in the title bar of the EnvForms program window You must create a sample list for each fraction in an ERM You must create a new sample list whenever you perform a new initial calibration on your system Only one initial calibration is allowed per sample list 1 Ifthe Edit Sample Information box is already displayed skip to step 8 to begin adding samples Otherwise select Create Sample List from the File menu Figure 5 Create Sample List Fraction Formset Volatile 1A 1E 248 Semivolatile 1BC 1F 2CD Pe
40. efault printer select Print from the File menu or click the Print button To exit the display window select Close from the File menu or click the Close button 7 Repeat step 4 through step 6 to generate other forms for the same sample list Click the Exit button when you are done 8 Repeat step 1 through step 7 to generate forms for other sample lists EnviroForms User s Guide Printing Forms To Customize Labels Used on Forms CmpdList units to Form 7D E EnviroForms User s Guide You can customize the final printed forms by specifying the labels for some of the data that appears on the forms Without customization the terminology on the reports complies with the US Environmental Protection Agency s March 1993 Contract Laboratory Program Statement of Work USEPA CLP SOW 1 Select Customize Formset Figure 11 from the Forms menu in the EnviroForms window Customize Formset Field Labels Approx Size CmpdList units to Form D E 0 001 of characters EPA Sample Number EPA SAMPLE NO 14 LabName labName 999 Contract Contract 9 LabCode Lab Code i Case Number CaseNo 79 SDG Number SDG No 8 SAS Number sas No B Footer Version OLM 02 0 8 Global Page Number I Figure 11 Customize Formset 2 Type in new values for any labels you want to change click OK when done Type in the multiplier used to convert instrument units to reporting units For example type 0 001 to
41. el Analyte Analyte on column Int std concentration Int std on column concentration ug L ng mL ug L ng mL ng per 5 0 mL ug L ng mL ng per 5 0 mL 1 1to5 25 to 125 20 500 2 2 to 10 50 to 250 20 500 5 5 to 25 125 to 625 20 500 10 10 to 50 250 to 1250 20 500 25 25 to 125 620 to 3125 20 500 Problem compounds such as ketones are at the higher level During calibration the volume purged is held constant at 5 0 or 25 0 mL The calibration equations are then expressed in terms 62 EnviroForms User s Guide Reference EnviroForms User s Guide Calculations for VOAs Analysis of concentration in the actual water sample Remember that 1 0 ug L 1 0 ng mL The calibration is not expressed in terms of nanograms on column Relative response factors RRF are computed using Equation 1 Again concentrations are used not nanograms on column RRF ae 1 is x where Ay Response of the analyte area Cis Concentration of the internal standard pg L ng mL Ais Response of the internal standard area Cy Concentration of analyte ug L ng mL SOW D 27 VOA Section 7 4 3 Equation 1 can be recast to solve for C the concentration of the unknown analyte C a a C 2 A x RRdF qnt The quantitation report gives Cano the concentration of the analyte if exactly 5 0 or 25 0 mL of the sample were purged The total amount of internal standard added to the sample in the purge tube l and desorbed onto the GC colum
42. en that the low soil CRQL is 10 ug Kg in a 5 0 mL sample Equation 8 can be solved for that Cont that gives 10 ug Kg In this case W is the nominal value of 5 g Again Con 10 ug L gives a the correct CRQL of 10 ug Kg for a low soil EnviroForms User s Guide Medium soil Calculations for VOAs Analysis Given that the medium soil CRQL is 1 250 ug Kg in a 5 0 mL sample Equation 10 can be solved for that Com that gives 1 250 ug Kg by setting all other terms to their nominal value 4 grams of sample 0 moisture 10 000 uL methanol extract volume and 100 uL methanol aliquot volume and solving for Cont Again Com 10 ug L gives the correct CRQL of 1 250 ug Kg for a medium soil When rounded off this becomes 1 300 pg Kg CROL calculations J flags EnviroForms User s Guide If the analyte is not found EnviroForms software will calculate a Corgi using Equation 11 and substitute that for Com into Equation 6 Equation 8 or Equation 10 as appropriate DL noc Corgi A 11 q M noc where Cor Value of Com to give the CRAL concentration DLaoc Value of Report Detection Limit on column in the Compound List Mnoc Ng on column Multiplier 5 or 25 mL in the Sample List The value for Report Detection Limit in the Compound List can be treated as an information only field The important field is the Ng on column field in the Sample List Each sample can give a different CRQL The CRQL is dependent on the actual sample size
43. he list select the compound by clicking it then click the Delete button You are prompted to confirm deletion of the current compound 5 To change the order that compounds appear in the list select the compound you want to move by clicking it then click the Cut button Select the new position in the list and click Paste The compound is inserted in the list above the current selection 6 To add a compound to the list use the Cut and Paste buttons If you have already cut a compound as described in step 5 select a position in the list for the new entry and click Paste The compound is inserted in the list above the current selection If you haven t already cut a compound as described in step 5 cut and paste a compound to its original position then paste the entry again where you want the new entry to appear in the list 44 EnviroForms User s Guide Setting Up Compound Lists Change the compound name simply by entering the new compound name in the already highlighted Name field Change other parameters as necessary by clicking a field to select it then type in the new information If you plan to change all parameters for the new compound click the New button to clear all the values from the old compound then type in the new values 7 GC data with two signals only To modify the compound information for a different signal click the Signal 1 2 button The compound information for signal 2 will be displayed When you are do
44. high The best workaround for this is to set a quant dilution factor of 0 2 and apply it to only the surrogates Then the number in the quant report will be one fifth of what it should be The dilution factor used in EnviroForms software will then correct this to the proper concentration Small dilution factors can give errors because the formats used in CSV MAC to write out QUANT CSV are limited to two decimal places The formats in CSV MAC may need to be changed to overcome this A calibration set up with a heated purge is a different calibration from a non heated purge The purging efficiencies will be quite different As such heated purge calibrations should exist in a different ERM from the multipoint calibration that does not use a heated purge Calibrating soils EnviroForms User s Guide The calibrations for a soil are always Sample Type Calibration Matrix Level Water and Percent Moisture 100 75 76 Calculations for VOAs Analysis Production run A production run consists of all injections into a given system while the multipoint curve is valid An ERM can continue as long as the initial multipoint curve is valid You do not need to create a new ERM for each days work EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide 7 Calculations for SVs Analysis Calibration 78 Water Samples 81 Soil Samples 83 SV CROLs 85 Water 85 Low soil 86 Medium soil
45. house use or you might want to produce forms with only a few key compounds of interest in addition to full reports EnviroForms User s Guide Setting Up Compound Lists To Load or Select a Compound List EnviroForms User s Guide The current environmental reporting method and sample list are displayed in the title bar of the EnviroForms program window To modify the compound list for the current sample list skip to the next section Otherwise follow the directions below to load or select a different compound list to associate with the current sample list and ERM When you create a new sample list or ERM as described in Chapter 3 you are automatically prompted to load a compound list 1 Ifthe Load Compound List box is already displayed skip to step 2 Otherwise select Load Compound List from the Edit menu Figure 8 Load Compound List Select a Source Data Analysis Method ERM Environmental Reporting Method Update Type Update Replace Append Figure 8 Load compound list 2 Select a Source for the compound list either a Data Analysis Method or an existing Environmental Reporting Method 3 Select the Update Type either Update Replace or Append then click OK 41 Setting Up Compound Lists 42 4 When the file select dialog box appears select a cmpdlist csv file in the method directory of interest for a Data Analysis method or the appropriate erm file for an Environmental Reporting Method the
46. identification of the column used to analyze the samples This value will be overwritten for individual samples if their quant csv file identifies the GC column The internal diameter of the GC column in millimeters This value will be overwritten for individual samples if their quant csv file identifies the internal diameter of the GC column Sample Preparation information All fractions Date Extracted Sample wt vol Units g or ml Dilution Factor EnviroForms User s Guide The date the samples were extracted The sample weight in grams or its volume in milliliters The units used for Sample wt vol Use G for grams or ML for milliliters The dilution factor used for the samples Use 1 if the samples were not diluted or concentrated 27 Setting Up Sample Lists Ng on column Multiplier Final Multiplier Used to correct for unit differences between Environmental Data Analysis and EnviroForms For example if you use parts per billion ppb units in EDA but want to report parts per million ppm on the environmental forms use a multiplier of 0 001 Also used to correct for the expected sample volume of water samples For example you have set up the method to use 25 mL samples For a standard sample you would enter Sample wt vol as 25 and Ng on column Multiplier as 25 If insufficient sample amount exists for a reanalysis and less that 15 mL is used enter 15 mL as the Sample wt vol and 25 mL as the Ng on column Mul
47. ion 35 To generate environmental forms 54 To load or select a compound list 39 To modify a compound list 41 To modify a sample list 33 To prepare data for forms production 14 To produce environmental forms an overview 16 To set default header information 23 Tune evaluation 15 Form 5 52 sample type 32 specifying results file for 30 tune csv file 30 U Units g or ml 25 Units ppm or ppb 26 57 Utilities menu 35 108 V Volatiles analyzing soil for 65 68 analyzing water for 62 64 calculations used for 60 61 Compound List Manager panel for 41 70 Edit Sample Information panel for 29 63 list of forms for 11 52 selecting as fraction type 21 28 specifying sample prep information for 26 volume purged 60 W Water expected sample volume 26 matrix type 24 pesticides analysis in 94 95 semivolatiles analysis in 79 80 volatiles analysis in 62 64 Windows 8 EnviroForms User s Guide ee Agilent Technologies Agilent Technologies Inc Printed in USA August 2003 G1701 90052
48. ions to include in this ERM analyze the samples for the next fraction and generate the input files required by EnviroForms Repeat step 3 and step 4 to set up sample lists and compound lists for this fraction then print the forms as described in step 5 EnviroForms User s Guide Agilent G1701DA MSD Prodoctivity Software EnviroQuant Mode EnviroForms User s Guide 3 Setting Up Sample Lists Setting Up Sample Lists 22 To Create a New ERM 23 To Set Default Header Information 25 Sample ID Type information 26 Contract Lab information 27 Sample Preparation information All fractions 27 Sample Preparation information Volatiles 28 Sample Preparation information Semivolatiles 28 Sample Preparation information Pesticides 29 To Create a Sample List 30 To Modify a Sample List 35 To Display or Print Sample Information 37 i Agilent Technologies 21 Setting Up Sample Lists Setting Up Sample Lists 22 Sample lists determine what samples will be reported on the environmental forms reports and specify the header information to be reported for each sample Set up a sample list in the EnviroForms software to match a set of samples analyzed in your lab often called a Sample Delivery Group Quantitative results for individual samples are copied to the sample list from the quant csv files generated in Environmental Data Analysis Separate sample lists are created in EnviroForms for volatile semi volatile and pesticide fracti
49. iroForms User s Guide Calculations for Pesticide Analysis CF Calibration factor for the MED level area per ng injected gt 1 Original sample volume extracted 1 000 mL The nanogram on column term Mpg 0 001 is needed if a quantitation report shows the LOW standard at 5 0 ng mL If the quantitation report shows the LOW standard at 0 005 ng uL the nanogram on column term is set to 1 97 Calculations for Pesticide Analysis Soil Samples Calibration is performed as described for a water sample The SOW specifies using Equation 25 to determine the concentration of the analyte in an unknown soil sample The SOW specifies using only what appears to be a Medium Soil sample preparation Reference SOW D17 PEST Section 6 2 A x V x Df ug Kg CF xV x W x D 25 where Ay Response of the analyte area V Concentrated extract volume 10 000 uL Df Dilution factor see definition in SOW CF Calibration factor for the MED level area per ng W Wet weight of sample extracted 30 g D Fractional solids 100 Moisture 100 Reference SOW D 53 SV Section 13 5 2 The equation that EnviroForms software will use to calculate the concentration of an analyte in a pesticide soil sample is derived from Equation 25 by first substituting Ci X Mhogo for A CF to give Equation 26 C xM xVvV x Df JK nt noc t 26 HEs Ke x WXD 26 98 EnviroForms User s Guide EnviroForms User s Guide Calculations
50. ironmental forms 52 56 example forms 12 52 multiple forms 55 sample information 35 36 Production run 74 89 102 Purge heated 26 73 volume 60 Q Quant csv file 30 Quantitation limits for pesticides 98 100 for semivolatiles 83 86 for volatiles 69 72 Quantitative results selecting file 30 updating 33 RAM recommended 8 Reanalysis 31 EnviroForms User s Guide Recovery from Florisil 47 from GPC 47 of matrix spike 46 of surrogate 45 Re Extraction 31 Reference Blank 24 Relative response factors RRF 61 77 Report Dectection Limit on column 45 Reporting Name 43 Reporting parameters 43 48 modifying 42 Resolution Check 47 Response factors calculating relative 61 77 specifying minimum 45 standard deviation in 45 S Sample Delivery Group specifying 25 Sample ID Type information 24 Sample list adding samples to 33 compound list and 20 38 creating 28 creating input file for 15 definition of 10 deleting samples from 33 initial calibration files and 20 28 73 89 102 loading 33 35 54 modifying 33 naming 28 printing 35 selecting fraction type for 21 28 setting up 20 viewing 35 Sample Name 31 Sample Preparation information 25 27 Sample Type 24 31 35 Sample wt vol 25 68 Index Samples adding to sample list 33 creating lists of 20 creating results files for 15 deleting from sample list 33 entering dilution fa
51. it or try to exit the panel To add a sample click the New Sample button and fill in the information as described earlier in this chapter To delete a sample select the sample by clicking it in the list then click the Delete button You are prompted to confirm deletion of the current sample To update the quantitative results for a sample from the same data file name click the Refresh Results button 4 GC data with two signals only To modify sample information for a different signal click the Signal 1 2 button The sample information for signal 2 will be displayed You can modify the Lab File Path the Contract Lab information and the Sample Preparation information You cannot select a 35 Setting Up Sample Lists quant file add a new sample or delete a sample Use the same button now labeled Signal 2 1 to return to the sample list for the first signal 5 When all the changes have been made to the sample list click the Exit button You are prompted to confirm saving changes to the last sample you modified 36 EnviroForms User s Guide Setting Up Sample Lists To Display or Print Sample Information You can display and print the information in the sample list in various ways as described below These procedures are for viewing data only see To Modify a Sample List on page 35 if you want to make any changes 1 Ifthe sample list you want to view is the one currently displayed in the title bar of the EnvForms wi
52. ity Software EnviroQuant Mode EnviroForms User s Guide 2 Getting Started What is Agilent EnviroForms Software 12 How it works 12 Forms available 13 Related products 14 Online help 15 To Prepare Data for Forms Production 16 Input files required for forms 16 To Produce Environmental Forms 19 i Agilent Technologies Getting Started What is Agilent EnviroForms Software Agilent EnviroForms software allows you to easily generate quantitative reports for your samples which include the following information e Quantitative results for up to 200 target compounds and for MS data only library search results for non target compounds found Form 1 e A surrogate recovery report which shows for each sample the analyte loss due to sample preparation Form 2 e A matrix spike report which shows the effect of the sample matrix water or soil on analyte recovery Form 3 e A blank report which correlates samples with their method blanks Form 4 e MS data only A tune evaluation report which documents the calibration and performance of the GC MS system Form 5 e An initial multi point calibration report Form 6 e A continuing single point calibration report Form 7 e An internal standard area and retention time stability report Form 8 e Pesticides only A Florisil cartridge check and GPC calibration Form 9 e Pesticides only An identification summary for single and multicomponent analytes Form 10
53. l samples and V is between 10 and 100 uL If the methanolic extract is diluted then Df will be calculated based on that dilution SOW D 37 VOA Section 8 2 2 5 The calculations used by EnviroForms software for the medium soil VOAs sample can be derived by substituting Cis X Myo for in Equation 9 expressing V in uL instead of mL and then substituting Cyn for the terms in Equation 2 to give Equation 10 Cc xM xVvV x Df ug Kg ee 10 Vx W x D where Cont Concentration from the quant report ug L Mnoc Ng on column Multiplier 5 or 25 mL V MeOH soil extract volume in the Sample Information panel 10 000 uL Df Dilution Factor in the Sample Information panel Va MeOH Aliquot Volume in the Sample Information pane 100 uL W Sample wt vol in the Sample Information panel 4 g D Fractional solids 100 Moisture 100 Percent moisture is the value entered in the Moisture field in the Sample Information panel for the sample EnviroForms User s Guide VOA CROLs EnviroForms User s Guide Calculations for VOAs Analysis The CRQLs that SOW C 2 specifies are based on a detection limit of 50 nanograms on column for each analyte in the method when the sample size is 5 0 mL The SOW gives a table that converts the 50 nanograms on column detection limit to the nominal CRQL concentration of the analyte in the original sample This is 10 ug L for water 10 ug Kg for low soil and 1250 ug Kg for medium
54. le Called Al in EDA Compound Type Select a compound type option from Table 7 Table 7 Compound type options Compound type Description Target A compound whose presence or absence in a sample must be reported ISTD Internal standard A compound added to the sample extract in a known concentration prior to analysis All concentration calculations for target compounds are made relative to the response of an internal standard Internal standards are also used to establish the retention time stability of a chromatographic system The expected retention time of a target compound is typically adjusted relative to the actual retention time of an internal standard This practice ensures accurate detection of target compounds Matrix Matrix spike compound This compound type includes special Matrix Spike fields on the right side of the dialog box A matrix spike is a compound that is added to an aliquot of a matrix water or soil and carried through the entire analytical procedure in order to measure the recovery properties of that procedure for that matrix Surr Surrogate compound This compound type includes special Surrogate fields on the right side of the dialog box A surrogate is a compound that is added prior to sample preparation to every blank unknown sample matrix spike matrix spike duplicate and standard in order to evaluate analytical efficiency by measuring the recovery of the surrogate compound Surrogate compounds
55. modify reporting parameters for individual compounds as described in Chapter 4 note cmpdlist csv is saved under the method file 4 After each unknown matrix spike and blank sample is analyzed quantitated and reviewed for correctness select Generate Report to Forms File from the Quant menu to create a quant csv file for each sample The analytical results in the quant csv files become part of the EnviroForms sample list as described in Chapter 3 Setting Up Sample Lists If a data file is requantitated or if the results are reintegrated manually generate a new quant csv file to reflect the updated results note quant csv is saved under the data file 5 MS data only Generate a tune csv file as follows For volatiles select Evaluate BFB to Forms File from the Tuner menu Select Tuner click on Evaluate BFB select Evaluate BFB to Forms File then click on OK Tune evaluation data is reported on Form 5A for volatile samples note Tune csv is saved under the data file For semi volatiles select Evaluate DFTPP to Forms File from the Tuner menu Select Tuner click on Evaluate DFTPP select Evaluate DFTPP to Forms File then click on OK Tune evaluation data is reported on Form 5A for volatile samples note Tune csv is saved under the data file Use Table 2 as a quick reminder of the files required by the EnviroForms software and the menu items used to generate them in the Environmental Data Analysis software EnviroForm
56. n click OK As with any Windows file selection dialog box you can also load the file by double clicking the file name of interest See Also To Modify a Compound List on page 43 EnviroForms User s Guide Setting Up Compound Lists To Modify a Compound List EnviroForms User s Guide Use this procedure to modify the compound list associated with the current sample list displayed in the title bar of the EnviroForms program window To modify the compound list for a different sample list or ERM load that sample list or ERM as described in Chapter 3 To associate a different compound list with the current sample list and ERM see To Load or Select a Compound List on page 41 1 Ifthe Compound List Manager box Figure 9 is already displayed skip to step 2 Otherwise click the Edit Compound List button on the toolbar of the EnviroForms window or select Edit Compound List from the Edit menu When you create a new sample list or ERM as described in Chapter 3 you are automatically prompted to load and modify a compound list gt Compound List Manager Volatile Compounds Hewlett Packard DAENVDEMO WOALIST M Bromochloromethane Minimum Response Factor Chloromethane Maximum RSD Bromomethane z Vinyl Chloride Maximum Response Factor Dev Chloroethane Report Detection Limit on column Methylene Chloride Calibration Range Upper Limit Acetone Carbon Disulfide Matrix Spike Information a n ue ale Matrix Spike Amount Dikas Ma
57. n is given in Equation 3 IL C xM 3 D l Concentration of the internal standard ug L ng mL Mnoc Ng on column Multiplier 5 or 25 mL 63 Calculations for VOAs Analysis Water Samples The SOW specifies using Equation 4 to determine the concentration of an analyte in the unknown sample Note that the number of nanograms of internal standard desorbed onto the GC column is specified rather than C the concentration of the internal standard g L SRST 4 where Ay Response of the analyte area I Amount of internal standard added nanograms on column Df Dilution factor Ais Response of the internal standard area RRF Relative response factor Vo Volume purged 5 or 25 mL Reference SOW D 42 VOA Section 10 3 64 EnviroForms User s Guide Calculations for VOAs Analysis Edit Volatile Sample Information panel EnviroForms User s Guide EnviroForms software uses the values set in the Sample Information panel Figure 12 In this example the user default value for Ng on column Multiplier Mpoo has been set to 25 0 mL The values entered for MeOH Soil Extract Vol and MeOH Aliquot Volume are for soil and are ignored when the sample type is water Sample ID Type _ Contract Lab _ Sample Name veikor itt Lab Name JHPTest LabSampleID Blank Lab Code Ha Lab File Name JBLwvo02D Contract fas0321 Lab FilePath D ENVDEM
58. n that the pesticide soil CRQL is 1 7 ug Kg Equation 27 can be solved for that C that gives a soil sample concentration of 1 7 ug Kg Set all other terms to their nominal value V 1 0 uL W 30 g V 10 000 uL Mnoc 0 001 Df 1 and solve for Cyn A Cont 5 ng mL 5 pg uL gives the correct CRQL of 1 7 1 66 ug Kg for a pesticide soil sample The term Moc 0 001 is needed when the on column detection limit is expressed in terms of picograms on column CROL calculations If the analyte is not found EnviroForms software will calculate a Coq using Equation 27 and substitute that for Cym in Equation 24 or Equation 26 as appropriate Corgi oe 27 noc where Cog Value of Can to give the CRAL concentration Dlooe Report Detection Limit on column 5 10 500 picograms Mnoc Ng on column Multiplier 0 001 in the Sample List EnviroForms User s Guide 101 Calculations for Pesticide Analysis 102 J flags The value for Report Detection Limit in the Compound List can be treated as an information only field The important field is the Ng on column Multiplier field in the Sample List Each sample can give a different CRQL The CRQL is dependent on the actual sample size the actual dry weight of the soil the final extract volumes and the dilution factor The J flag will be assigned any time that Cont is lt Dlpoc Mnoc If the LOW standard is reporting analytes at 5 00 ng mL the on column detection
59. n the numerator and C in the denominator Since C nanograms on column volume injected the ratio Ci Cy is the same if C is expressed in ng mL or if it is expressed in terms of nanograms on column EnviroForms User s Guide Soil Samples Reference EnviroForms User s Guide Calculations for SVs Analysis Calibration is performed as described for a water sample The SOW specifies using Equation 18 to determine the concentration of the analyte in an unknown soil sample Although the SOW specifies a Soil Low and Soil Medium sample preparation the SOW specifies only a single equation for determining the concentration of analytes in an unknown Semivolatiles Soil Sediment sample A xI x V x Df ug Kg A x RRF xV xW xD 18 where Ay Response of the analyte area I Amount of internal standard added 40 nanograms on column V Concentrated extract volume 1 000 uL Df Dilution factor see definition in SOW Ais Response of the internal standard area RRF Relative response factor V Volume of concentrated extract injected 2 0 uL W Wet weight of sample extracted g D Fractional solids 100 Moisture 100 SOW D 53 SV Section 8 2 The equation that EnviroForms software will use to calculate the concentration of a Semivolatiles Soil Sample is derived from Equation 18 by first substituting Ci X M for then substituting Cyn for the elements of Equation 16 to give Equation 19 83 Calculati
60. ndow skip to step 2 Otherwise To load a different sample list in the same ERM click the Sample List button on the toolbar of the EnvForms program window To load a sample list in a different ERM select Open ERM from the File menu To see an example of each listing use the sample data provided in demo erm 2 Select an item Table 6 from the Utilities menu Table6 Utilities Name Displays Display Sample Information Header information for a selected sample Display Sample List List of samples including the Lab File ID EPA Sample ID Lab Sample ID date acquired data received and date due for each sample Display Header Summary Header information for all samples in the list in a single table Display Sample Group Summary List of samples grouped by sample type including data file names and the date and time each file was acquired EnviroForms User s Guide 37 Setting Up Sample Lists 3 The first page of the table appears on the screen Use the page arrow buttons at the lower left part of the window to view more data Next page CC KO Pie Previous page 4 To print the list to the Windows default printer select Print from the File menu or click the Print button 5 To exit the display window select Close from the File menu or click the Close button 38 EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide 4 Setting Up Compound Lists Set
61. ne use the same button now labeled Signal 2 1 to return to the compound list for the first signal 8 Optional Click the Print button to print the compound list See To Display or Print a Compound List on page 51 to view or print the list after you have exited the Compound List Manager dialog box 9 When all the changes have been made to the compound list click the Exit button You are prompted to confirm saving changes to the last compound you modifed Compound reporting parameters Name Reporting Name CAS Number EnviroForms User s Guide The compound reporting parameters are described below Most of the information in these fields is filled in from Page 2 of the Edit Compounds dialog box in the EnviroQuant Data Analysis EDA software The equivalent parameter name in EDA is given when it differs The name of the compound as it appears in the quantitation database of the Environmental Data Analysis Software The name of the compound as it is to appear on the environmental form optional Chemical Abstracts Service Number used on Forms 1A and 1B You can enter any text here as a proprietary registry number 45 Setting Up Compound Lists Abbrev Surrogates and internal standards require three letter compound name abbreviations on their respective forms Forms 2A 2B 2C and 2D for surrogates and Forms 8A and 8B for internal standards Use upper case letters enclosed in parentheses BEN for benzene for examp
62. ograms on column The SOW gives a table that converts the 100 200 or 500 picograms on column detection limits to the nominal CRQL concentration of the analyte in the original sample This is 1 0 2 0 or 5 0 mg L for water and 33 0 67 0 or 170 0 mg Kg for soil samples SOW C 8 In the Compound List Manager dialog box you can enter these values as Report Detection Limit on column These values are also entered on Page 2 of the quantitation database Report Detection Limit on column in EnviroForms Compound List Manager is field N6 on Page 2 of the quantitation database in Environmental Data Analysis The value for Report Detection Limit on column can be treated as an information only field The important field is the Ng on column Multiplier field in the Sample List You must adjust the Report Detection Limit on column numbers DL to match how the standards are reported EnviroForms User s Guide Calculations for Pesticide Analysis Water Given that the pesticide water CRQL is 0 05 ug L Equation 24 can be solved for that Cy that gives a water sample concentration of 0 05 pg L Set all other terms to their nominal value Vo 1 000 mL V 1 0 uL V 10 000 uL M 0 001 Df 1 and solve for Con A Con 5 ng mL 5 pg L gives the correct CRQL of 0 050 ug L for a pesticide water sample The term Myo 0 001 is needed when the on column detection limit is expressed in terms of picograms on column Soil Give
63. ons Sample lists are associated with a compound list and reporting criteria for a fraction A sample list can contain many samples but is limited to one set of initial calibration files A new sample list must be created when a new initial calibration is performed for a fraction As described in Chapter 4 compound lists are also created for volatile semi volatile and pesticide fractions within an environmental reporting method ERM In this way you can specify which compounds are reported on the final printed forms for a particular group of samples To facilitate the process of creating a sample list much of the header information that is often the same for all samples in a group can be specified only once This header information is then supplied automatically and can be customized as necessary for individual samples EnviroForms User s Guide To Create a New ERM Setting Up Sample Lists An environmental reporting method determines which samples and compounds will be reported on the volatile semi volatile and pesticide environmental forms Figure 3 describes how to set up a new environmental reporting method ERM for a group of samples The compound lists are created and managed in the Compound List Manager dialog box Create ERM gt Select fraction gt Figure 3 Creating an ERM EnviroForms User s Guide Create compound list and specify reporting parameters a Specif
64. ons for SVs Analysis 84 gqnt noc t 19 Concentration from the quant report ug L ng L or Nanograms on column from the quant report Ng on column Multiplier 1 or 2 uL Concentrated extract volume in Sample Information panel 1 000 uL Dilution factor in Sample Information panel see definition in SOW Volume of concentrated extract injected 2 0 uL Wet weight of sample extracted in Sample Information panel 9 Fractional solids 100 Moisture 100 If a dilution is used it is mandatory that additional internal standard spiking solution be added to the extract to bring the concentration of the internal standards up to 20 ng uL prior to analysis The value that you enter for Myo is 2 if Cg is reporting standards at 10 25 40 60 and 80 ng uL It is Lif Com is reporting standards at 20 50 80 120 and 160 nanograms on column Percent moisture is the value entered in the Moisture field in the Sample Information panel for the sample EnviroForms User s Guide SV CROLs Reference Water EnviroForms User s Guide Calculations for SVs Analysis The CRQLs that the SOW specifies are based on a detection limit of 20 nanograms on column Eight semivolatile analytes have a CRQL based on 100 nanograms on column SOW C 4 The SOW gives a table that converts the 20 or 100 nanograms on column detection limit to the nominal CRQL concentration of the analyte in the original sample This is 10 or 50
65. ows 2000 or XP Configuration e Either Windows 2000 Professional Service Pack 3 SP3 or later or Windows XP Service Pack la SP 1a or later must be installed as the operating system ChemStation software is not supported on Windows 2000 Professional Server software or on Windows NT If you are upgrading from an earlier revision of the MSD ChemStation Software you may have to change operating systems See Upgrading from Earlier Versions in Chapter 5 e The MSD ChemStation software requires that all disk partitions on the MSD ChemStation computer hard drives be formatted for NTFS and that active file systems are uncompressed EnviroForms User s Guide EnviroForms User s Guide Introduction Minimize the Windows 2000 or XP desktop configuration as follows minimum desktop icons no screen saver enabled no background or tray applications Disable unused computer hardware such as USB in the BIOS The TCP IP networking protocols must be installed and enabled NetBEUI needs only to be installed if printer and or files are to be shared Microsoft Internet Explorer 5 5 or greater is required Some devices which Windows 2000 or XP supports are not supported by MSD ChemStation software for example magneto optical drives and external CD ROM writers Do not use these devices A Windows 2000 or XP repair disk must be created during installation and recovery disks must be created Windows 2000 or XP Computer Hardware Conside
66. ples and compounds for each additional fraction 10 See Chapter 5 to generate forms for the sample lists defined in this procedure 24 EnviroForms User s Guide To Set Default Header Information EnviroForms User s Guide Setting Up Sample Lists Use the following procedure to specify sample header information to be used as defaults for all samples All these default values can be modified for individual samples as described in To Create a Sample List on page 30 and To Modify a Sample List on page 35 1 Ifthe Edit Default Sample Information box is already displayed see step 2 to fill in the panel Otherwise select Default Sample Values from the Samples menu The example in Figure 4 is for volatiles The information requested for semivolatiles and pesticides is slightly different Edit Default Volatile Sample Information Sample ID Type Date Received fas Date Due 2 5 91 Sample Type Sample E Matrix Water el Reference Blank Y OB Sample Preparation Date Extracted paza Sample wt vol jo Units g or mi Mc Moisture not decanted 100 Heated Purge Y N E Contract Lab Lab Name Lab Code Contract Case No SAS No SDG No Instrument ID GC Column GC Column ID MeOH Soil Extract Vol MeOH Aliquot Volume Dilution Factor Ng on column Multiplier Final Mialoplhier Figure 4 Default header information AP Tes JHP401 pon jewoor HPS DB5Ms Jo 20
67. r from an existing ERM If necessary add or delete compounds or modify compound reporting parameters and limits See Chapter 4 for more information Add samples to the sample list including calibration samples method blanks matrix spikes and unknown samples analyzed for this fraction See Chapter 3 for more information To speed up the process you can set up the compound list and the ERM as described in the above steps after only some of the samples have been analyzed You can then add additional samples as they are analyzed by repeating step 1 and step 3 only But wait until the entire set of samples for a fraction is complete before printing any forms as described in step 5 19 Getting Started 20 5 Print the desired environmental forms for this sample list as described in Chapter 5 Wait until all the related samples have been analyzed and the required input files generated from EnviroQuant Data Analysis before you attempt to print any environmental forms because each form requires information from several input files Although some forms might be correctly generated with only some of the input files present it is more likely that individual forms based on a partial set of input files would be incomplete or inaccurate For example an Organics Analysis Data Sheet Form 1 produced before the method blank has been processed would not be able to flag compounds that were detected in the blank If you have additional fract
68. rations The minimum requirements for operation of the MSD ChemStation Software are A Pentium III computer 450 MHz or faster BIOS which supports Windows 2000 with Service Pack 3 or later or Windows XP with Service Pack la or later All power saving features of the BIOS disabled All power saving programs removed An SVGA color monitor 1024 x 800 x 65 536 colors video display resolution maximum supported resolution is 1600 x 1200 x 16 000 000 colors 64 MB of RAM additional RAM required for multiple instruments 128 MB for dual MSD acquisition 256 MB for quad GC acquisition Introduction At least 800 MB of hard disk storage MSD ChemStation program files use 115 MB e A CD ROM drive e A sound card and speakers or headphones e A Windows 2000 or XP supported mouse e A data backup device is strongly recommended e Printer Support All DeskJet printers These printers are not tested and are not supported LaserJet 4050 and 4100 These printers are not tested but inherit support because they were tested on older revisions The supported drivers are PCL 5e or PCL 6 revision 5 02 LaserJet 2200d and 2300dn These printers are fully tested and are supported The supported drivers are Post Script revision 5 02 e MSD communication card A PCI Network Interface Card for LAN based systems A PCI GPIB Control Card 82350A or 82350B for GPIB based systems 10 EnviroForms User s Guide Agilent G1701DA MSD Productiv
69. s Sample Name jvekor ooo Lab Name JHPTet o VBLKOT LabSampleID Blanki Lab Code Hao Lab File Name BLWVO02D Contract ji Lab File Path D ENVDEMO VOAD Case No o i i Date Received 1 5 91 SAS No Date Due Rsa ooo SDG No Jvswoor Sample Type Method Blank E Instrument ID HPS Matrix War E GC Column pess Reference Blank VBLKOT E GC Column ID fo Sample Preparation Date Extracted fi 13 91 Sample wt vol 25 New Sample Units g or ml ML MeOH Soil Extract Vol Update Results Moisture not decanted fi 00 Me0H Aliquot Volume Heated Purge Y N E Dilution Factor E Ng on column Multiplier 25 Final Multiplier 1 00000 Figure 6 Edit sample information 31 Setting Up Sample Lists 32 8 To start the sample list read in the quantitative results for the first sample by clicking the Quant File button Figure 7 Select a CSV File File Name Directories quant cs c woadata slwyv001_d cis E gt envdemo E gt voadata gt slwv001 d List Files of Type Drives Samples quant cs c chemstation Figure 7 Selecting a data file 9 When the list of data files appears select the file of interest for this sample by double clicking the name of its data file in the list on the right side of the panel The csv files generated for this sample are displayed on the left side of the panel 10 Use the list box in the lower left corner of the screen to select th
70. s User s Guide 74 75 89 91 Soil Samples 98 Pesticide CROLs 100 Single component analytes 100 Multi component analytes 100 Water 101 Soil 101 CROL calculations 101 Jflags 102 Other Factors 103 Ng on column Multiplier 103 GPC Cleanup Y N 103 Further Concerns 104 Dilutions and surrogates 104 Calibrating soils 104 Productionrun 104 Index EnviroForms User s Guide Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User s Guide 1 Introduction EnviroForms Software 8 What youneed 8 Ee Agilent Technologies Introduction EnviroForms Software What you need Agilent EnviroForms software allows you to produce environmental reports based on analytical data from an Agilent GC or MS ChemStation The reports contain quantitative results for target compounds quality control data for surrogates blanks matrix spikes and calibration samples For analyses by mass spectrometer you can also report library search results for non target compounds and tune evaluation data For GC analyses you can report Florisil and GPC calibration data and a Pesticide Identification Summary The terminology used on the printed forms complies with the US Environmental Protection Agency s Contract Laboratory Program Statement of Work March 1993 but can be easily customized for other applications as well The requirements for optimum performance of EnviroForms software are listed below Microsoft Wind
71. s User s Guide Calculations for VOAs Analysis Calibration is performed as described for a water sample The SOW specifies using Equation 7 to determine the concentration of an analyte in a low soil sample A x1 ug Kg A x RRF x W x D 7 where Ax Response of the analyte area I Amount of internal standard added ng Ais Dilution factor RRF Relative response factor W Wet weight of sample added to the purge tube g D Fractional solids 100 Moisture 100 SOW D 43 VOA Section 10 3 The calculations used by EnviroForms software for the low soil VOAs sample can be derived from Equation 7 by substituting Cis X Mnoc for then substituting Com for the elements of Equation 2 in Equation 7 to give Equation 8 C xM w ke gt 8 W xD where Cont Concentration from the quant report ug L ng mL Mnoc Ng on column Multiplier 5 or 25 mL W Wet weight from the Sample Information panel 4 g D Fractional solids 100 Moisture 100 67 Calculations for VOAs Analysis Percent moisture is the value entered in the Moisture field in the Sample Information panel for the sample Note that no dilution factor is used in Equation 7 and Equation 8 When Form 1 is printed for a low soil sample the Soil Extract Volume and Soil Aliquot Volume fields are blank The dilution factor is 1 by default 68 EnviroForms User s Guide Medium Soil EnviroForms User s Guide Calculations for VOAs Analysis
72. s User s Guide 17 Getting Started 18 Table 2 Files and menu items Type of data Menu item Compound list Initial calibration Continuing calibrations Blank unknown and matrix spikes samples Tune evaluation MS data only Make Compound List for Forms Report Calibration Table to Forms File Evaluate Data File as Continuing Cal to Forms File Generate Reports to Forms File Evaluate BFB DFTPP to Forms File See Also To Produce Environmental Forms on page 19 EnviroForms User s Guide Getting Started To Produce Environmental Forms The form production process is outlined in Figure 2 Analyze sample and generate gt Create new ERM Create sample list Add samples to i and compounds to s Print forms z sample list report list input files Continue with next fraction Figure 2 Producing environmental forms 1 EnviroForms User s Guide Analyze the samples for the first fraction and generate the input files required by EnviroForms as described in To Prepare Data for Forms Production on page 16 Create an environmental reporting method ERM for the samples of interest See Chapter 3 for more information Create a new sample list for the group of samples to be reported If this is the first sample list for a fraction you are prompted to load a compound list from Environmental Data Analysis o
73. s case is also numerically equal to the concentration in the original sample assuming 100 extraction efficiency 79 Calculations for SVs Analysis The amount of internal standard injected on column ng is given in Equation 13 I Cis A V Cis x M noc 13 where Cis Concentration of the internal standard ng pL V Volume of standard purged 2 uL Moc Ng on column Multiplier 2 uL The term Myo is the value that you entered for the Ng on column Multiplier This value will be either 1 or 2 uL depending on whether your calibration is set up in terms of concentration in the extract or nanograms on column The latter case is preferable the concentration should be expressed in terms of nanograms on column 80 EnviroForms User s Guide Water Samples Reference EnviroForms User s Guide Calculations for SVs Analysis The SOW specifies using Equation 14 to determine the concentration of an analyte in a semivolatiles water sample A xI xV xD ey ita eel tan ser 14 Ais X RRF x Vo x V where Ax Response of the analyte area lz Amount of internal standard added 40 nanograms on column V Concentrated extract volume 1 000 uL Df Dilution factor see definition in SOW Ais Response of the internal standard area RRF Relative response factor Vo Original sample volume extracted 1 000 mL V Volume of concentrated extract injected 2 0 uL SOW D 52 SV Section 8 2 The equation used to calcula
74. soil In the Compound List Manager dialog box Figure 13 on the next page you can enter these values as Report Detection Limit on column These values are also entered on Page 2 of the quantitation database Report Detection Limit on column in EnviroForms Compound List Manager is field N6 on Page 2 of the quantitation database in Environmental Data Analysis 71 Calculations for VOAs Analysis 72 Compound List Manager for Volatiles Water Low soil Compound List Manager Volatile Compounds Hewlett Packard DAENYVDEMO WOALIST M Bromochloromethane Minimum Response Factor Chloromethane E Maximum RSD Bromomethane Vinyl Chloride Maximum Response Factor Dev Chloroethane Report Detection Limit on column Methylene Chloride Acetone Carbon Disulfide Matrix Spike Information K amaata Matrix Spike Amount Pisati ataandaa Matrix Water low high Method Name _ 1 1 Dichloroethene Matrix Water RPD Reporting Name fi 1 Dichloroethene Matrix Soil low high Cas 75 35 4 Abbrev Matrix Soil RPD e Type Calibration Range Upper Limit C Target Matix Sur C ISTD Cut Paste New Delete Print Exit Help Figure 13 Compound List Manager Given that the volatile water CRQL is 10 ug L in a 5 0 mL sample Equation 6 can be solved for that Cyn that gives 10 ug L Obviously Con 10 ug L gives a CRQL of 10 ug L This is 50 nanograms on column divided by M the default Ng on column Multiplier Giv
75. st Optional To change the labels for some data on the forms see To Customize Labels Used on Forms on page 59 in this chapter Click the Preview Forms button on the toolbar of the EnvForms program window or select Preview Print Forms Figure 10 on the Forms menu Preview Print Forms F Check box to print Multiple Forms Fann Seleckon FileName EPASample ID Lab Sample ID INSTBLKP D PBLKO1 Instrument Blank 1 Organics Analysis Data Sheet MSPIKE2P D ROLOO2MS Matrix Spike k MSPKDP2P D ROL002MSD Matrix Spike Duplicate o2 Suo eee PERFO0IP D PEMOT Performance Eval C 3 Matrix Spike Duplicate Recovery PERF002P D PEMO2 Performance Eval O Mahad Blank Sumay RSLVOOIP D RESCOI Resolution Check E SAMPLE1P D ROLOO1 Sample 1 SAMPLE2P D ROLOO2 Sample 2 O G inital Colbration Data SAMPLE3P D ROL003 Sample 3 Z Continuing Calibration Check 8 Analytical Sequence Summary 9 Pesticide Cleanup Summary 10 Pesticide Aroclor Identification Figure 10 Preview Print Forms EnviroForms User s Guide EnviroForms User s Guide Printing Forms 4 Select the forms and samples to print a Single form single sample Leave the check box in the upper left corner of the panel blank to generate a single form for a selected sample Select the form from the left side of the screen Select the sample of interest from the right side of the screen except for Forms 2 and 5 which don t require sample selection
76. sticide 1D 2EF Sample List Name voa 0 14 1994 2 Figure 5 Create sample list 2 Select the fraction type for the samples Volatile Semivolatile or Pesticide 3 Type in a name for the Sample List or accept the default name which is based on the fraction type and the current date then click OK 4 When the message The Compound List is empty Read Compound List appears click the Yes button You can load a compound list from either an Environmental Data Analysis method or an existing ERM EnviroForms User s Guide EnviroForms User s Guide Setting Up Sample Lists 5 When you have finished creating the compound list click the Edit Samples button on the toolbar of the EnviroForms window or select Enter Edit Sample from the Samples menu To specify default header information for the sample list see To Set Default Header Information on page 25 Once specified the default header information will appear for each sample you add to the sample list The header information can be modified for individual samples as described later in step 13 When the Edit Sample Information box appears use it to set up the sample list as described in Steps 8 through 16 The box shown in Figure 6 is for volatiles The information requested for semivolatiles and pesticides is slightly different Edit Yolatile Sample Information Sample ID Type _ Contract Lab _ Sample
77. te the concentration of a semivolatiles water sample is derived from Equation 14 by substituting Cj X Mihoc for to give Equation 15 LE AX Cis X Mnoc X V x Df 15 Aj X RRF x Vox V Equation 12 can be rearranged to give Equation 16 A g Cis Cx A RRE Cam Us 16 81 Calculations for SVs Analysis 82 Substituting C into Equation 15 for the elements of Equation 16 gives Equation 17 which EnviroForms software uses to calculate the concentration of an analyte in an unknown semivolatiles water sample Cant Mnoc X Vi Df nt noc g L Li noc i gt 17 H Vo x V l where Cont Concentration from the quant report ug L ng mL or Nanograms on column from the quant report Moc Ng on column Multiplier 1 or 2 uL V Concentrated extract volume in Sample Information panel 1 000 uL Df Dilution factor in Sample Information panel see definition in SOW Vo Original sample volume extracted 1 000 mL V Volume of concentrated extract injected 2 0 uL When you enter sample information from within EnviroForms software you must set the Ng on column Multiplier Mpoo to agree with the way you set up the calibration The value that you enter for Mpoc is 2 if Cyn is reporting standards at 10 25 40 60 and 80 ng uL It is Lif Com is reporting standards at 20 50 80 120 and 160 nanograms on column The RRFs reported are independent of the way you set up the calibration Equation 12 has Cj i
78. ting Up Compound Lists 40 To Load or Selecta Compound List 41 To Modify a Compound List 43 Compound reporting parameters 45 Additional fields for pesticides only all compound types 49 To Display or Printa Compound List 51 Apg Agilent Technologies 39 Setting Up Compound Lists Setting Up Compound Lists 40 Compound lists determine what compounds will be reported on the environmental forms reports for a particular sample list within an environmental reporting method A compound list also defines the parameters for reporting the results for each compound such as compound type Target Matrix Surrogate ISTD detection limits and cutoff values for flagging data minimum response factor minimum relative standard deviation and maximum response factor deviation The initial compound list comes from the cmpdlist csv file generated from Environmental Data Analysis as described in Chapter 2 Compound lists are stored as part of an environmental reporting method ERM in EnviroForms From within EnviroForms a compound list can be modified and printed as described in this chapter You may want to vary the reporting parameters in the compound list and save different versions of the list in different ERMs When these different compound lists are applied to the same sample list you can produce more than one set of reports for the same samples For example you might want to have a more stringent set of quality control parameters for in
79. tiplier For display only Sample Preparation information Volatiles Moisture not decanted Heated Purge Y N MeOH Soil Extract Volume MeOH Aliquot Volume The percentage of moisture not decanted rounded to the nearest percent Don t type in the sign Click to select this box if a heated purge cycle was used Leave this box blank if the purge cycle was not heated Since the purging efficiencies are different between heated and non heated purges all samples in a list should be run with the same purge technique The volume in microliters used to extract a soil sample The volume in microliters of the aliquot taken from a soil extract Sample Preparation information Semivolatiles Moisture Decanted Y N pH 28 The percentage of moisture in the samples rounded to the nearest percent Don t type in the sign Select this box if the samples were decanted Leave this box blank if the samples were not decanted The pH of the samples to one decimal place accuracy EnviroForms User s Guide Setting Up Sample Lists GPC Cleanup Select this box if the samples were cleaned up with GPC Leave Y N this box blank if the samples were not cleaned up with GPC Extract Vol The volume of the concentrated extract in microliters Injection The injection volume in microliters Volume Sample Preparation information Pesticides Moisture The percentage of moisture in the samples rounded to the n
80. tris Water low high Method Name fi A Dichloroethene Matrix Water RPD Reporting Name fi A Dichloroethene Matrix Soil low high CAS 75 35 4 Abbrev Matrix Soil RPD Compound Type C Taget Matis CO Sur C ISTD Figure 9 Compound list manager The reporting parameters for the first compound are displayed Note that the compounds will be reported on the forms in the order they appear in the compound list To change the order of the compounds see step 5 43 Setting Up Compound Lists 2 Modify the reporting parameters for the current compound if necessary Select a Compound Type of either Target Matrix Surr or ISTD Notice that additional fields appear on the panel when you select the Matrix or Surr compound types Modify any reporting parameter by clicking a field to select it then type in the new information To clear all fields for the current compound click the New button then type in the new information for each field For more information see the description of the compound reporting parameters at the end of this section or in online help 3 To modify the reporting parameters for a different compound click the compound of interest in the list The reporting parameters for the selected compound are displayed and can be modified as described in step 2 You are asked to confirm saving changes to the previous compound before the parameters for the next compound are displayed 4 To remove a compound from t
81. ug L for water 330 or 1700 333 or 1666 ug Kg for low soil preparation and 10 000 or 50 000 mg Kg for medium soil preparation In the Compound List Manager dialog box you can enter these values as Report Detection Limit on column These values are also entered on Page 2 of the quantitation database Report Detection Limit on column in Compound List Manager is field N6 on Page 2 in the quantitation database in Environmental Data Analysis Given that the semivolatile water CRQL is 10 ug L Equation 17 can be solved for that C that gives 10 ug L Set all terms to their nominal value for a water sample Myo 2 uL V 1 000 uL Df 1 V 2 uL and Vo 1000 mL and solve for Cyn A Cont 10 ug L gives the correct CRQL of 10 ug L in a water sample When the calibration is set up to report standards at 10 25 40 60 and 80 ng uL Moc must be set to 2 and Report Detection Limit on column must be set to 20 or 100 Thus the Cym term used when no analyte is found is the value of DLjg Mngc When the calibration is set up to report standards at 20 50 80 120 and 160 nanograms on column Moc must be set to 1 and Report Detection Limit on column DL must be set to 10 or 50 Then the C term used when no analyte is found is again the value of Dlyg Mnoc 85 Calculations for SVs Analysis 86 Low soil Medium soil Given that the low soil CRQL is 333 ug Kg in the original sample Equation 19 can be solved
82. using GPC cleanup and the dilution factor 87 Calculations for SVs Analysis J flags 88 The J flag will be assigned any time that Cam is lt DLpg Mnoc The Report Detection Limit on column must be set to 20 or 100 if Mnoc 2 If Mnoc 1 Report Detection Limit on column must be set to 10 or 50 EnviroForms User s Guide Calculations for SVs Analysis Other Factors EnviroForms software uses some other factors in its calculations that affect the numbers reported on the Forms See Figure 14 Sample ID Type _ Contract Lab _ Samples Sample Name RFO Lab Name JHPLab o Aa Lab SampleID Sampe Lab Code Hao Lab File Name SLWBOO1D Contract fw12894 Lab File Path C ENVDEMO BNAD Case No oOo Date Received LO o yO SAS No e Date Due o i i SDG No Fao CC Sample Type Sampe InstumentID HP5 Matrix Solow E GC Column JHP5MS Reference Blank SBLKO0T e GC Column ID 02 Sample Preparation Date Extracted GPC Cleanup Y N Sample wt vol Units g or mil Extract Volume Moisture Injection Volume Decanted N Dilution Factor pH Ng on column Multiplier Final Multiples Figure 14 Semivolatile sample information panel Ng on column Multiplier This appears on the Edit Sample Information screen and is used to convert ng mL to ng uL as needed in Equation 24 and Equation 26 Set M to 0 001 if the LOW standard is labeled as 5
83. y default sample information Create sample list Select Create ERM from the File menu Type in a name for the new ERM then click OK Unless you specify otherwise the new ERM will be stored in the msdchem envforms directory When the Create Sample List dialog box appears select the fraction type for the samples Volatile Semivolatile or Pesticide Type in a name for the Sample List or accept the default name which is based on the fraction type and the current date then click OK When the message The Compound List is empty Read Compound List appears click the Yes button You can load a compound list from either an Environmental Data Analysis method or an existing ERM For more information on creating and customizing compound lists see Chapter 4 When you have finished creating the compound list and specifying the compound reporting parameters click the Edit Samples button on the toolbar of the EnviroForms window When the message Edit Default Sample Header values appears click the Yes button Specify header information to be used as 23 Setting Up Sample Lists default information for all samples as described in To Set Default Header Information on page 25 8 Create the list of samples as described in To Create a Sample List on page 30 9 Optional To create sample lists for other fractions select Create Sample List from the File menu Repeat step 3 through step 8 above to define the sam
84. zation of the forms or want to output the forms electronically purchase Microsoft Access You can use Access to further manipulate the files or to export them in various formats These exported files can then be used as input to a variety of software programs such as word processing or desktop publishing applications EnviroForms User s Guide Getting Started Online help You can access online help for the Environmental Forms software in any of the following ways e Select a topic from the Help menu e Click the Help button in any dialog box The EDA Configuration panel is used to customize the operation and functionality of the Data Analysis software The configuration may be changed at any time by selecting File EDA Configuration in Data Analysis EnviroForms User s Guide 15 Getting Started To Prepare Data for Forms Production 16 Input files required for forms Use the Enviromental Data Analysis program EDA as described below to prepare data for forms production This procedure assumes you have already analyzed the samples of interest including a tune evaluation sample calibration samples at each calibration level blanks surrogates and quality control samples using the Agilent MSD Productivity ChemStation EDA software provides both the individual data files for all samples analyzed as well as the list of compounds in the quantitation database The MSD Productivity ChemStation can be configured for up to four

Agilent G1701DA MSD Productivity Software EnviroQuant Mode EnviroForms User Guide

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