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Agilent 1200 Series Dual Loop Autosampler User Guide(1)

1. E T ze l z A SIS i CAL f TT F ram NE 23 D Figure 10 Flow Connections to and from the Dual Loop Autosampler Since the ports of the 10 port valve are very close to each other fitting screws of different lengths are provided in the DLA accessories kit The shortest fittings screws are used for the connection that doesn t require frequent access like twin seat tubings and waste tubings The sample loops are fitted with medium length fittings The very long fittings are intended for connecting the capillaries from the pump and to the column or column thermostat To change sample loop it may be necessary to disconnect inlet and outlet capillaries first 56 Agilent 1200 Series Dual Loop Autosampler User s Guide CAUTION Installing the Dual Loop Autosampler 3 After replacing sample loops or capillaries on the 10 port valve check if all other connections are still tight Due to the very tight space it may happen the an adjacent fitting gets loose String a back ferrule a front ferrule and long fitting screw onto the orange 0 5 x 800 mm capillary from the accessories kit 2 Put the end of the capillary completely into port 9 of the 10 port valve 3 Tighten the fitting screw while pushing the tubing in to the valve port 4 When finger tight use a 1 4 inch wrench or the RheoTool to tighten the
2. CAN DC Out ewer AC Power CAN cable to next module Figure 7 Cable Connections The Dual Loop Autosampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF To disconnect the Dual Loop Autosampler from the line unplug the power cord The power will supply still uses some power even switch at the front panel is turned OFF I CAUTION The Dual Loop Autosampler thermostat requires 25 cm 10 inch space on each for sufficient air circulation To avoid personal injury keep fingers away from the needle area during Dual Loop Autosampler operation Do not attempt to insert or remove a vial or a plate when the needle is positioned Agilent 1200 Series Dual Loop Autosampler User s Guide 53 3 Installing the Dual Loop Autosampler Installation aaan Dual Loop Autosampler Thermostat cable BEBE pnnama CAN bus AC Power Figure
3. 102 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 10 Pull excessive loop tubing out at the metering head side 11 Push guide tubing and plastic chain into the needle Y assembly until they hit the hard stop ics HA 12 Slide the needle assembly into the guide on the needle On completion of this procedure carrier assembly and lock the needle assembly lever y Turn the needle carrier assembly inwards Re install the side cover Re install the tray s in the tray base Turn the instrument ON Close the front door Agilent 1200 Series Dual Loop Autosampler User s Guide 103 7 Maintaining the Dual Loop Autosampler Replacing the Buffer Loop Frequency When contaminated worn out or visibly damaged Typically once per year Tools required Wrench open end 4mm PN 8710 1534 supplied in accessory kit Tweezers supplied in accessory kit Cutter or sharp knife Parts Required Twin needle assembly PN G2258 87306 2mm fitting kit PN 5065 9950 Upper loop tubing assembly PN G2258 87300 only every 3rd time WARNING Thoroughly follow the described installation procedures to maximize the lifetime of the buffer loop tubing and to avoid potential spills Regularly inspect the tu
4. 8 Slide the needle assembly into the guide of the needle carrier and close the lever latch On completion of this procedure Re install the tray s in the tray base Re install the side cover Start the instrument Close the front cover Perform a DLA Transport Alignment 114 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 Replacing Seal or Piston of the Metering Unit Frequency When leaky or defective Tools required Pozidrive no 1 screwdriver supplied in accessory kit 4mm hex key with T handle PN 8710 2392 supplied in accessory kit Parts required Metering piston seal PN 0905 1599 Metering piston PN G2258 60003 WARNING When opening capillary or tube fittings solvents may leak out Flush the metering unit with appropriate non toxic solvents e g water before carrying out this procedure Before beginning this procedure 1 Disconnect the 1 8 plastic tubing from the metering head Disconnect the buffer loop from the PEEK fitting N N Move the piston into the Change Metering Device position see Maintenance Functions in the Service Manual Agilent 1200 Series Dual Loop Autosampler User s Guide 115 7 Maintaining the Dual Loop Autosampler 2 Remove the purge plug and the two 3mm screws that hold the valve bracket
5. 3 Carefully pull the flush valve and the buffer loop extension out Beware of the solenoid cable 4 Using a 4mm hex key unscrew the two metering head screws Pull the metering head assembly off the metering unit and place it upside down on a clean surface ae 5 Unscrew the two screws on the rear side of the metering head assembly with a 3mm hex key 116 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 6 Separate the piston from the piston seal by holding down the metering head while pulling guide ring and spring housing upwards 7 Remove the metering seal from the metering head Place the new seal spring upwards onto a clean bench Hold the metering head parallel to the bench surface and press it with gentle force onto the seal 8 Push the piston out of the guide ring and the spring housing Clean it with toothpaste if dirty Replace it if it is scratched or damaged So 9 Stack guide ring adapter and spring housing Slide the piston into the stack Agilent 1200 Series Dual Loop Autosampler User s Guide 117 7 Maintaining the Dual Loop Autosampler 10 Place the stack onto the metering head Push the piston 11 Check the orientation of the metering head assembly and down to align it with the seal correct if required Tubing port lef
6. Position the needle arm of the sampler into the Change Needle Seat position see Maintenance Functions in the Service Manual Turn the instrument OFF Open the front door and pop the left side cover out to get good access to the needle arm 100 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 2 Using a knife or cutter cut the transparent plastic tubing directly behind the fitting screw J Vy i 3 Remove the needle assembly by pressing the blue plastic handles of the needle assembly together while sliding it towards the inside of the sampler Aa 5 Using a4mm wrench remove the fitting screw and pull the cut off end of the plastic tubing out of the needle fitting Discard the ferrule and the compression ring The fitting screw can be reused 101 Agilent 1200 Series Dual Loop Autosampler User s Guide 7 Maintaining the Dual Loop Autosampler 6 Pull approx 5cm 2 of buffer loop out of the guide 7 push the loop tubing through the new needle assembly tubing Push from the other end if necessary 8 String ferrule screw new ferrule ring and new ferrule 9 Tighten the ferrule screw carefully while pressing the together Place the end of the buffer loop into the needle buffer loop firmly into the fitting to avoid dead volume fitting
7. WARNING Be careful when handling the needle assembly The pointed tip of the needle may cause personal injury when touched Before beginning this procedure 1 Pull the needle lever latch down Remove the needle assembly by pressing plastic clamps together while pushing the needle assembly backwards Position the transport unit of the Dual Loop Autosampler in the Home Position see Maintenance Functions in the Service Manual Remove all installed trays from the tray base Position the transport unit of the Dual Loop Autosampler in the Change Needle Carrier position see Maintenance Functions in the Service Manual and turn the instrument off 112 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 2 Unplug the flex board from the transport assembly Va i A Tms 3 Remove the three hexagonal screws at the bottom side of the needle carrier with a 2mm hex key SN 4 Take the needle carrier off 5 Hold the new needle carrier against the transport assembly arm and rotate it until it is aligned Agilent 1200 Series Dual Loop Autosampler User s Guide 113 7 Maintaining the Dual Loop Autosampler 7 Plug the flex board into the connector on the transport assembly arm KK
8. in to the sample loop any vessel or to waste Valid entries are 350 ul min 50 000 ul min Agilent 1200 Series Dual Loop Autosampler User s Guide 79 4 Using the Dual Loop Autosampler CAUTION Draw Position Allows to change the needle position in sample vessels in the range of 10 mm 10 mm lower to 50 mm 50 mm higher The use of this parameter requires careful consideration as improper use may damage needle and sample vessel Equilibration Time Defines for how many seconds the sampler waits for pressure equilibration in sample vial and buffer loop until it proceeds with the next injection step This parameter allows to optimize reproducibility for viscous samples higher draw speeds and large injection volumes Store Temperature Stores the temperature of the sample compartment as part of the data file requires optional Agilent 1200 Series Sample Thermostat Vial Well Bottom Sensing If this box is ticked the sampler auto detects the bottom of the sample vessel A few conditions are not suitable for this function The needle moves off center into the well with spherical or conical bottom The vial plate well plate has not been defined properly The latter point is of special importance because the sampler moves the needle at high speed into the vessel and slows down only a few millimeters above the bottom At low speed the needle moves further down until the sampler notices an increase in motor current Using Bottom Sen
9. screw another 1 2 to 3 4 turn to seal the fitting pressure tight Repeat steps 1 to 4 with the other end of the capillary but use the pump outlet fitting for swaging Use one of the shorter fitting screws This procedure ensures that dead volumes are avoided no matter what pump is used String a back ferrule a front ferrule and long fitting screw onto the blue 0 25 x 800 mm capillary from the accessories kit 7 Put the end of the capillary completely into port 4 of the 10 port valve 8 Tighten the fitting screw while pushing the tubing in to the valve port When finger tight use a 1 4 inch wrench or the RheoTool to tighten the screw another 1 2 to 3 4 turn to seal the fitting pressure tight 10 Swage the other end of the blue capillary to the fitting of your column accordingly Use one of the shorter fitting screws Agilent 1200 Series Dual Loop Autosampler User s Guide 57 3 Installing the Dual Loop Autosampler Flushing the Metering Device 58 Initial Priming by Backflushing with External Pump When required At initial installation or if the metering head is filled with air e g after replacing seal or piston Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit Parts required none Air in the metering device results in unpredictable injection volumes and large peak area standard deviation It is essential to remove any gas from the metering head the solenoid valve and all tubing before an
10. Loop Autosampler in the Home Position see Maintenance Functions in the Service Manual 1 I Remove the tray from the tray base Position the transport unit of the Dual Loop Autosampler in the Change Parts Position see Maintenance Functions in the Service Manual _ Turn the instrument OFF e 108 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 2 Pull the two waste tubings out of the twin seat adapter 3 Use a flat blade screwdriver to lever the twin seat from the seat adapter Attach the screwdriver blade to the solid rim at the bottom of the seat and not to the top ring as it will break off 4 Remove the seat tubings from the old seat with a 1 4 wrench and attach them to the new seat assembly Mind the correct orientation the wider ID tubings goes into the rear seat On completion of this procedure Push the seat into the seat adapter until it snaps in place Mind the guide notch Reconnect the seat tubings to ports 2 and 6 of the 10 port valve Fix the two valve waste tubings into the holes of the twin seat adapter Re install the tray s in the tray base Start the instrument Close the front cover Agilent 1200 Series Dual Loop Autosampler User s Gui
11. e G2258A Thermostatted Dual Loop Autosampler preparative scale thermostatisation is achieved by adding a G1330B thermostat Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 The term thermostatted Dual Loop Autosampler preparative scale refers to these Agilent 1200 Series modules in this manual Unless otherwise stated all information in this section is valid for both models Table1 Delivery Checklist for the G2258A Dual Loop Autosampler Description Quantity Dual Loop Autosampler module with inlet waste tubing assemblies 1 Power cord local Matching the sockets in your country or region 1 Accessory kit see below 1 Table 2 Delivery Checklist for the G1330B Thermostat Module optional Description Quantity Thermostat Module 1 Power cord local Matching the sockets in your country or region 1 Accessory kit thermostat module see below 1 Accessory Kits Each sampler comes with an Accessory Kit which contains the tools and consumables needed to install the LC system and make the first runs Table3 Dual Loop Autosampler Accessory Kit Contents G2258 68705 Description Quantity Part Number 1 16 front ferrule 316 stainless steel 6 0100 0043 re order 5180 4108 pack of 10 1 16 back ferrule 316 stainless steel 6 0100 0044 re order 5180 4114 pack of 10 Vial plate 54 x 2 ml 1 5022 6502 re order G2258 68700 pack of 6 Agilent 1200 Series Dual
12. bottom to the center of the first hole A1 H Column shift Offset in mm to Y when the rows are not straight but staggered J Well depth Distance in mm from the top of the up to 45mm plate to the bottom of the well If you use well plates with inlets caps and septa you have to specify the distance from the top of the septa to the bottom of the inlets 66 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Table5 Configuring Well plate Types continued Location Description Definition Limits Well X size Size of the well in x direction Plate min 3 7 mm length If you use well plates with min 3 0 mm with inlets caps and septa you have to position accuracy specify the x size of the septa alignment micro scale Well Y size Size of the well in y direction Plate down to 3 7 mm width If you use well plates with min 3 0 mm with inlets caps and septa you have to position accuracy specify the y size of the septa alignment micro scale Bottom size For round wells the relative of the 1 0 cylindrical well top and bottom of the well 0 0 conical well Square Click in the field to specify whether Yes rectangular the well is rectangular or round No round oval Is well plate Click in this field to specify ifthisis Yes well plate or a well plate or not Relevant for continuous flow operation MALDI Target No Vial Tray or Eppendorf tray The distances need
13. 25 Influence of enject speed on recovery Recovery 120 5 100 98 98 93 100 ul 89 88 80 a 60 aa Eject speed 1000 pL min Eject speed 20000 L min Eject speed 5000 L min Eject speed 30000 L min Eject speed 10000 L min Eject speed 40000 L min Eject speed 15000 L min Figure 25 Influence of eject speed on recovery draw speed 20000 pL min e For best recovery results the sample draw speed should not exceed 20000 uL min e Lower eject speeds yield better recoveries The default eject speed is 10000 uL min however for best recovery results this value should be lowered further e Highly viscous rinse solvents like DMSO for example yield lower recoveries even with low eject speeds Therefore we recommend using a mixture of DMSO acetonitrile 50 50 v v instead Sample loop overfill factor for complete loop filling Depending on the size of the used sample loop the loop overfill factor should range between at least 3 5 times the volume of the used loop in order to achieve best possible reproducibility of the results with minimum deviations Agilent 1200 Series Dual Loop Autosampler User s Guide 83 4 Using the Dual Loop Autosampler Achieving the best recovery results with the Agilent 1200 Series Dual Loop Autosampler PS Sample loop fill factor for partial loop fill Figure 26 shows the result of sev
14. 5061 3303 pack of 10 126 Agilent 1200 Series Dual Loop Autosampler User s Guide Easy Repair Parts 8 Table 12 Description Quantit Part Number y Fitting screw 1 16 inch extra long 3 G1156 22402 re order 5065 4454 pack of 10 screws front ferrules back ferrules Air channel adapter 1 G1329 43200 ESD wrist strap 1 9300 1408 Drawing tube assembly for flush solvent 1 G2258 87307 Agilent 1200 Series Dual Loop Autosampler User s Guide 127 8 Easy Repair Parts Dual Loop Autosampler Accessory Kit 128 Agilent 1200 Series Dual Loop Autosampler User s Guide This chapter gives Safety Information for the use of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide A Safety Information Safety Information 130 Lithium Batteries Information 133 Radio Interference 134 Sound Emission 135 Solvent Information 136 Agilent Technologies on Internet 137 ee Agilent Technologies 129 A Safety Information Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument pr
15. 8 Connections at the Rear of the Thermostatted Dual Loop Autosampler 54 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Flow Connections to the Dual Loop Autosampler Preparation Dual Loop Autosampler is installed in the LC system Parts required Parts from the accessory kit see Dual Loop Autosampler Accessory Kit on page 126 WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used WARNING Regularly inspect the buffer loop tubing and exchange it if worn out or showing visible signs of damage WARNING This instrument should only be used with solvents that have an ignition temperature higher than 200 C Connect the flush solvent tubing from the accessory kit to the lower left port of the flush valve Screw the bottle cap onto the flush solvent bottle and place it into the solvent cabinet of the LC system Figure 9 Position of the Flush Solvent Port of the Flush Valve Agilent 1200 Series Dual Loop Autosampler User s Guide 55 3 Installing the Dual Loop Autosampler
16. Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to Repairing the Dual Loop Autosampler 6 Cleaning the Dual Loop Autosampler The autosampler covers should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth from which liquid could drip into the instrument or organic solvents as they may damage the plastic cover WARNING Dripping water into the instrument may cause a shock hazard or damage to the instrument Using the ESD Strap CAUTION Electronic boards are sensitive to electrostatic discharge ESD In order to prevent damage always use the ESD strap supplied in the accessory kit 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 28 Using the ESD Strap Agilent 1200 Series Dual Loop Autosampler User s Guide 93 6 Introduction to Repairing the Dual Loop Autosampler Introduction to Repairing the Dual Loop Autosampler 94 Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 7 Maintaining the Dual Loop Autosampler Maintenance Procedures and Simple Repairs 96 Replacing the Buffer Loop 104 Replacing
17. Loop Autosampler ee Agilent Technologies 37 3 Installing the Dual Loop Autosampler Unpacking the Dual Loop Autosampler CAUTION If you need to ship the Dual Loop Autosampler at a later date always use the shipping protection foam parts see Special Transport Unit Positions on page 68 Damaged Packaging CAUTION Upon receipt of your autosampler inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged keep them until the contents have been checked for completeness and the Dual Loop Autosampler has been mechanically and electrically checked If the shipping container or cushioning material are damaged notify the carrier and keep the shipping material for the carrier s inspection If there are signs of damage to the autosampler please do not attempt to install the module Delivery Checklist 38 Ensure all parts and materials have been delivered with the Dual Loop Autosampler For this compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Two versions of the Agilent 1200 Series Dual Loop Autosampler are available G2258A Dual Loop Autosampler preparative scale designed for flow rates from 1ml min to 100 ml min It can be used with a wide variety of vials and well plates see Consumables on page 122 Fraction Collector
18. Loop Autosampler User s Guide 39 3 40 Installing the Dual Loop Autosampler Table3 Dual Loop Autosampler Accessory Kit Contents G2258 68705 continued Description Quantity Part Number Vial plate 15 x 6 ml 1 5022 6539 re order 5065 9949 pack of 6 Tubing assembly 2 5063 6527 Flexible capillary 0 5 x 800 mm no 1 5065 9926 fittings Flexible capillary 0 25 x 800 mm no 1 5065 9930 fittings CAN cable 1 m 1 5181 1519 Wrench open end 1 4 5 16 inch 2 8710 0510 Wrench open end 4mm 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4mm 150 mm long with 1 8710 2392 T handle Hex key 2 0 mm 1 8710 2438 Hex key driver 3 32 inch 1 8710 2462 Fitting screw 1 16 inch 3 79814 22406 re order 5061 3303 pack of 10 Fitting screw 1 16 inch extra long 3 G1156 22402 Air channel adapter ESD wrist strap Drawing tube assembly for flush solvent re order 5065 4454 pack of 10 screws front ferrules back ferrules G1329 43200 9300 1408 G2258 87307 The Accessory kit G1330 68705 shown in Table 4 is shipped with the G1330B thermostat module if the thermostat module was ordered Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler Table 4 Optional Thermostat Module Accessory Kit Contents G1330 68705 Description Quantity Part Number Waste tubing 1 2 m 1 5062 2463 Cable thermostat module to Dual Loop Autosampler 1 G1330 81600 1 Reorder gives 5m Agi
19. Peristaltic Pump lt To Column Figure 3 Plumbing Diagram of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 Table 1 Connections to the 10 Port Valve Port Connecting to 1 Waste tubing short s2 Back seat tubing large 3 Upper loop eA Outlet capillary to column a Lower loop 6 Front seat tubing short 7 Waste tubing long 8 lower loop 9 Inlet capillary from pump 10 Upper loop Agilent 1200 Series Dual Loop Autosampler User s Guide 15 1 Introduction to the Dual Loop ALS The Injection Sequence Idle or Run Position flush valve buffer loop with buffer loop extension metering unit flush solvent upper sampler front seat loop back seat lower sample loop Figure 4 10 port valve in Mainpass This is the standard position of the 10 port valve The mobile phase coming from the pump flows through the active sample loop in this case the upper one to the HPLC column The sample loop is thereby thoroughly flushed out 16 Agilent 1200 Series Dual Loop Autosampler User s Guide CAUTION Introduction to the Dual Loop ALS 1 Switching to Bypass and Drawing a Dead Volume Compensation Plug Figure 5 10 port valve in Bypass The 10 port valve switches to bypass mode The solvent flow from pump to detector is bypassed through the second sample loop The flush valve is activated and co
20. Supported Trays 70 Unsupported Trays 70 Operating the Dual Loop Sampler with Agilent ChemStation 71 Operating the Dual Loop Sampler with Agilent ChemStation 71 Navigation in Agilent ChemStation 71 Achieving the best recovery results with the Agilent 1200 Series Dual Loop Autosampler PS 81 This chapter describes the Usage of the Dual Loop Autosampler ee Agilent Technologies 4 Using the Dual Loop Autosampler General Comments about Sample Trays Supported Trays CAUTION The Dual Loop Autosampler recognizes the installed tray automatically Two supported trays are available Standard 2 well plate tray PN G1367 60001 This tray can hold up to two well plates or vial plates The maximum vessel height is 48mm Well plate tray 2 well plates 10 vials supports 50 mm plates PN G2258 60001 This new tray can hold up to two well plates or vial plates The sample plates are positioned two millimeters lower than in the standard sample tray This allows the use of vessels with a maximum height of 50mm Other trays are not supported with the Dual Loop Autosampler because their use may cause damages to the needle under special circumstances These damages are not covered by instrument warranty Unsupported Trays 70 The following vial trays are not supported with the G2258A Dual Loop Autosampler Std tray for 100 x 2ml vials PN G1313 44500 Std tray for 100 x 2ml vials thermostat able PN G1329 60001 Half tray for 40 x 2m
21. User s Guide List of Recommended Well Plates and Vial Plates Table9 Recommended plates Easy Repair Parts 8 Description Rows Columns Plate height Volume pl Part Number Package 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 96DeepNunc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96Nunc 8 12 14 3 400 No Agilent PN 96 Agilent conical 8 12 14 3 150 5042 8502 25 54 x 2 ml vials 6 9 27 2000 G2255 68700 6 15 x 6 ml vials 3 5 35 6000 5065 9949 6 Agilent 1200 Series Dual Loop Autosampler User s Guide 123 8 Easy Repair Parts List of Recommended Vials Caps and Septa Table 10 Recommended vial caps and septa Description Rows Columns Plate height Volume pl Part Number Package 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 96DeepNunc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96Nunc 8 12 14 3 400 No Agilent PN 96 Agilent conical 8 12 14 3 150 5042 8502 25 54 x 2 ml vials 6 9 27 2000 G2255 68700 6 15 x 6 ml vials 3 5 35 6000 5065 9949 6 124
22. at the main power switch Disconnect cables from the interface board connectors 3 Loosen the screws Slide out the interface board from the Dual Loop Autosampler 4 Install the interface board Secure the screws 5 Reconnect the cables to the board connectors Screws Figure 29 Exchanging the Interface Board 120 Agilent 1200 Series Dual Loop Autosampler User s Guide This chapter describes the Consumables and Accessories of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide 8 Easy Repair Parts Consumables 122 List of Supported Plate Trays 122 List of Recommended Well Plates and Vial Plates 123 List of Recommended Vials Caps and Septa 124 Sample Loops for the G2258A 10 port Valve 125 Dual Loop Autosampler Accessory Kit 126 ee Agilent Technologies 121 8 Easy Repair Parts Consumables List of Supported Plate Trays Table8 Dual Loop Autosampler Vial Trays and Tray Base Item Description Part Number 2 Tray for 2 plates 10 x 2 ml vials max height 50mm G2258 60011 2 Screws for springs 0515 0866 3 Spring G1313 09101 4 Spring stud 0570 1574 5 Tray base includes items 4 5 6 G1329 60000 6 Adapter air channel G1329 43200 Plug channel not shown G1367 47200 7 Vial plate for 15 x 6 ml vials pack of 6 5065 9949 8 Vial plate for 54 x 2 ml vials pack of 6 G2255 68700 Figure 30 Vial trays and Tray Base 122 Agilent 1200 Series Dual Loop Autosampler
23. data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met In This Guide This manual contains technical reference information about the Agilent 1200 Series Dual Loop Autosampler preparative scale The manual describes the following 1 introduction to the Dual Loop ALS This chapter gives an introduction to the Dual Loop Autosampler 2 Site Requirements and Specifications This chapter describes the Site Requirements and Specifications of the Dual Loop Autosampler 3 Installing the Dual Loop Autosampler This chapter describes the Installation of the Dual Loop Autosampler 4 Using the Dual Loop Autosampler This chapter describes the Usage of the Dual Loop Autosampler 5 Troubleshooting Overview This chapter gives an Overview to Troubleshooting the Dual Loop Autosampler 6 Introduction to Repairing the Dual Loop Autosampler This chapter gives an Introduction to Repairing the Dual Loop Autosampler 7 Maintaining the Dual Loop Autosampler This chapter describes the Maintenance and Simple Repairs of the Dual Loop Autosampler 8 Easy Repair Parts This chapter desc
24. in the same method 76 Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Fill loop partially with Use this injection mode if the intended injection volume is smaller than the volume of the selected sample loop Partial loop filling is a useful mode if only limited quantities of sample are available and diminished peak area accuracy and reproducibility are not critical For an in depth discussion see Loop Filling Modes on page 26 Overfillloop This is the preferred mode of operation if you are aiming for highest peak area accuracy and reproducibility The disadvantage is that sample is wasted for overfilling the sample loop Typical overfill factors for best results are 3 5 See also Loop Filling Modes on page 26 Standard Injection Checking this button makes the autosampler use the default injection sequence which is a good choice for most applications Injection with Needle Wash When this radio button is clicked the Needle Wash parameters on the right hand side of the input screen become accessible Injector Program Check this box if you want to use an individual series of injection steps called Injector Program Click Edit to open the injector program editor Injector programs are a powerful way to combine most sampler functions and execute them sequentially as part of the ChemStation method x i function Amount p Oestinahon 1 evect sj foe ea Program 1 abbr 8 Com
25. o mj o Autosampler Detector O Om seme MM l ee w eB o CAN Bus cable Fraction TEE Collector Calan ee Compartment iT E al Figure 2 Recommended Stack Configuration Analytical System Rear View NOTE If a G1330B ALS thermostat is part of the system it must always be installed underneath the Dual Loop Autosampler see I on page 53 44 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Optimizing the Stack Configuration Preparative System Solvent Compartment Preparative Pump A Column Organizer Preparative Pump B E UV Vis Detector Autosampler Fraction Collector Figure 3 Recommended Stack Configuration Preparative System Front View NOTE For information about connecting a G1946C D LC MSD or a Non 1200 or Non UV Vis i detector to the system please refer to the User s Guide for Purification High Throughput System PN G2262 90003 or to the Installation Note for the Mass Based Fraction Collection Kit PN G1968 90000 Agilent 1200 Series Dual Loop Autosampler User s Guide 45 3 Installing the Dual Loop Autosampler Optimizing the Stack Configuration Solvent Compartment Prepara
26. produced by the Xenon flash WARNING A warning alerts you to situations that could cause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions 132 Agilent 1200 Series Dual Loop Autosampler User s Guide Safety Information A Lithium Batteries Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Lithiumbatteri Eksplosionsfare ved fejlagtic handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 12
27. terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or asa commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will Agilent 1200 Series Dual Loop Autosampler User s Guide receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important
28. to be measured with high precision It is recommended to use a caliper If possible contact the vendor of non predefined well plates to obtain these dimensions Agilent 1200 Series Dual Loop Autosampler User s Guide 67 3 Installing the Dual Loop Autosampler Special Transport Unit Positions 68 Transporting the Dual Loop Autosampler When moving the Dual Loop Autosampler inside the laboratory no special precautions are needed However if the Dual Loop Autosampler needs to be shipped to another location via carrier ensure V The transport assembly is in the park position Use the Agilent ChemStation or the Control Module for this command To move the arm to the park position 1 Switch to the Diagnosis view of the Agilent ChemStation and select Dual Loop ALS gt Maintenance Positions from the Maintenance menu 2 In the upcoming dialog box click the Park Arm button V The vial tray and the sample transport mechanism is secured with the transport protection foam Maintenance Positions Maintenance and repairs require to move certain assemblies of the Dual Loop Autosampler into dedicated maintenance positions Access to the maintenance positions Chemstation Diagnosis View Maintenance Dual Loop ALS Maintenance Positions Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 4 Using the Dual Loop Autosampler General Comments about Sample Trays 70
29. 00 Series Dual Loop Autosampler User s Guide 133 A Safety Information Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 134 Agilent 1200 Series Dual Loop Autosampler User s Guide Safety Information A Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test Agilent 1200 Series Dual Loop Autosampler User s Guide 135 A Safety Information Solvent Information Observe the following recommendations on the use of solvents This instrument should only be used with solvents that have an ignition temperature higher than 200 C 136 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents
30. Agilent 1200 Series Dual Loop Autosampler Preparative Scale User s Guide aa Agilent Technologies Notices Agilent Technologies Inc 2006 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Microsoft is a U S registered trademark of Microsoft Corporation Manual Part Number G2258 90010 Edition 02 06 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these
31. Agilent 1200 Series Dual Loop Autosampler User s Guide Easy Repair Parts 8 Sample Loops for the G2258A 10 port Valve Table 11 Sample Loops for the G2258A 10 port Valve Description ID mm Part Number 10 pl sample loop 0 30 0100 1923 20 ul sample loop 0 30 0100 1922 50 ul sample loop 0 51 0100 1924 e 100 pl sample loop 0 51 0100 1921 e 200 pl sample loop 0 76 0101 1247 500 ul sample loop 0 76 0101 1246 1mlsample loop 0 76 0101 1245 2ml sample loop 1 00 0101 1244 5 ml sample loop 1 00 0101 1243 Agilent 1200 Series Dual Loop Autosampler User s Guide 125 8 Easy Repair Parts Dual Loop Autosampler Accessory Kit Table 12 Description Quantit Part Number y 1 16 front ferrule 316 stainless steel 6 0100 0043 re order 5180 4108 pack of 10 1 16 back ferrule 316 stainless steel 6 0100 0044 re order 5180 4114 pack of 10 Vial plate 54 x 2 ml 1 5022 6502 re order G2258 68700 pack of 6 Vial plate 15 x 6 ml 1 5022 6539 re order 5065 9949 pack of 6 Tubing assembly 2 5063 6527 Flexible capillary 0 5 x 800 mm no fittings 1 5065 9926 Flexible capillary 0 25 x 800 mm no 1 5065 9930 fittings CAN cable 1 m 1 5181 1519 Wrench open end 1 4 5 16 inch 2 8710 0510 Wrench open end 4mm 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4mm 150 mm long with T handle 1 8710 2392 Hex key 2 0 mm 1 8710 2438 Hex key driver 3 32 inch 1 8710 2462 Fitting screw 1 16 inch 3 79814 22406 re order
32. Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt S2COCly 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Agilent 1200 Series Dual Loop Autosampler User s Guide Safety Information A e Solutions containing strong complex forming agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will pr
33. ach injection The rinse volume which is calculated from the injection volume and a user defined factor should be at least 300 uL e The syringe should be purged at least 5 times using the Purge Syringe command from the Instrument More Injector menu prior to the first run of a sequence Flush solvent The flush solvent is used to flush the needle s exterior prior to the injection of the sample Therefore a solvent should be used in which the sample is readily soluble For a list of solvents compatible with the tubing of the peristaltic pump see Table 6 below Table 6 Solvents compatible with tubings Solvent PharMed Silicone Acetic acid gt 5 A B A Fully compatible Acetone D D Acetonitrile A B Minor reaction e g slight Hexane Heptane c F corrosion or discoloration NH4 acetate C C Not recommended for Ethanol C B continuous use Formic acid A C Swelling shrinkage loss of strength MeOH D A D Severe reaction not Propanol C A recommended for use Trichloroacetic acid D D Water A A Pre installed t Can be ordered 5042 8507 82 Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Sample draw and eject speed Due to the push through design of the dual loop autosampler the sample draw and eject speed influence the recovery Increasing the draw speed has only a minor effect however the influence of the eject speed has a much higher impact as shown in Figure
34. al Plates on page 123 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the Agilent ChemStation or the Control Module column Figure 16 Well plate Dimensions straight 64 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler Configuring Wellplate Types Figure 17 Well plate Dimensions staggered Agilent 1200 Series Dual Loop Autosampler User s Guide 65 3 Installing the Dual Loop Autosampler Table5 Configuring Well plate Types Location Description Definition Limits Rows Number of rows on the plate up to 16 Columns Number of columns on the plate up to 24 Volume Volume in pl of a sample vessel A Row distance Distance in mm between the center of two rows B Column distance Distance in mm between the center of two columns C Plate length X size in mm at the bottom ofthe 127 75 0 25 mm plate SBS Standard D Plate width Y size in mm at the bottom of the 85 50 0 25 mm plate SBS Standard E Plate height Size in mm from the bottom to the upto 47 mm top of the plate If you use well plates with inlets caps and septa you have to specify the distance from the bottom to the top of the caps F Column offset Distance in mm from the left edge bottom to the center of the first hole A1 G Row offset Distance in mm from the back edge
35. analysis is started The following procedures explain for this is achieved effectively Steps 1 7 are only necessary if metering head and tubings are completely dry e g during installation or after replacing metering seal or piston y oO ol A Fill the flush solvent bottle of the Dual Loop Sampler with 250 ml Isopropanol Remove the blue purge plug from the metering head assembly Connect the pump of the LC system e g G1310A G1311A G1312A or G1361A witha 0 5 mm ID capillary to this purge port Put the solvent intake tubing of the pump into a vessel with Isopropanol Turn the pump on and start flushing at 5 ml min until the solvent drains from on of the 10 port valve waste tubings Accelerate the pump to 10 ml min max for another 3 5 min Switch OFF the pump flow Disconnect the capillary and close the purge port with the blue plug Place the flush solvent bottle into your solvent cabinet Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Flushing the Metering Device Purging the Syringe NOTE The syringe purge function of the autosampler works best if the flush solvent bottle is located slightly above the sampler We recommend to keep it in the solvent cabinet Avoid placing it onto the lab bench as this will result in an underpressure in the tubing and may generate air bubble in the metering head Dual Loup Sampler online k Method amp Run Control fie RunControl instr
36. bings and exchange them if they are worn out or show visible signs of damage Before beginning this procedure Position the transport unit of the Dual Loop Autosampler in the Home Position see Maintenance Functions in the Service Manual Remove the sample tray from the tray base Position the needle arm of the sampler into the Change Loop Capillary position see Maintenance Functions in the Service Manual Turn the instrument OFF Open the front door and pop the left side cover out to get good access to the needle arm 1 Pull the lever latch of the needle assembly down to expose the needle fitting 104 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 2 Using a knife or cutter cut the transparent plastic tubing directly behind the fitting screw 3 Remove the needle assembly by pressing the blue plastic handles of the needle assembly together while sliding it towards the inside of the sampler Aa 5 Using a4mm wrench remove the fitting screw and pull the cut off end of the plastic tubing out of the needle fitting Discard the ferrule and the compression ring The fitting screw can be reused 105 Agilent 1200 Series Dual Loop Autosampler User s Guide 7 Maintaining the Dual Loop Autosampler 6 Disconnect the other end of the buff
37. bubbles the syringe will not flushed out automatically Due to the higher compressibility of air compared to liquids precision and reproducibility of the sampler will suffer significantly if any gas bubbles are present in the metering device The use of premixed 5 10 MeOH in water minimizes the formation of air bubbles over time Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Flushing the Metering Device e In order to keep sample contamination low you will have to rinse the syringe loop prior to next injection This is not necessary for repetitive injections How to flush the Syringe In Agilent ChemStation right click the DLA icon and select Purge Syringe from the drop down menu see Figure 13 Accessing the Purge Syringe Dialog In the appearing dialog box the number of syringe purge cycles can be defined Figure 14 Purge Syringe Dialog Agilent 1200 Series Dual Loop Autosampler User s Guide 61 3 Installing the Dual Loop Autosampler CAUTION This command is interactive Add how often you wish to purge the syringe and press Start In the example above the syringe will draw and eject five times its total volume of 5 ml flush solvent at the maximum speed of 50 ml min The flush solvent is ejected directly into waste The waste position is in front of the needle wash port How much Flush Solvent is needed Whenever changing the flush solvent to a solvent with dif
38. ctor This function allows to start the run from an external device which is connected to APG start stop connector of any 1200 module When Use External Injector is activated the autosampler icon turn grey in Agilent ChemStation GUI and the sampler is ignored Purge Syringe This command allows to purge the metering device interactively Doing so is e g required when changing the flush solvent or to remove air bubbles For an in depth discussion of this function read Purging the Syringe on page 59 Solvent Bottle Filling Tracks the filling of the flush solvent bottle and inhibits further analysis or generates an error condition when certain solvent limits are reached Agilent 1200 Series Dual Loop Autosampler User s Guide 75 4 Using the Dual Loop Autosampler Operating the Dual Loop Sampler with Agilent ChemStation Setting Method Parameters in Agilent ChemStation All method related autosampler parameters are consolidated in one expandable screen It can be accessed from the Method amp Run Control screen in two different ways e By left clicking the autosampler icon in the graphical user interface By pulling down the Instrument menu and selecting Set up Injector Figure 22 ChemStation DLA Setup Screen Injection Parameters In this part of the screen the active loop and the loop filling mode are defined Use Loop Select the sample loop you want to use with the method Mind that it is not possible to use both loops
39. d fuses and shorting of fuse holders must be avoided WARNING Any adjustment maintenance and repair of the opened instrument under voltage is forbidden WARNING Disconnect the instrument from the line and unplug the power cord before maintenance Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Agilent 1200 Series Dual Loop Autosampler User s Guide 131 A Safety Information Safety Symbols Table 13 shows safety symbols used on the instrument and in the manuals Table 13 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage f Indicates dangerous voltages Indicates a protected conductor terminal lamp used in this product Always turn the xenon flash lamp off before removing it gt Eye damage may result from directly viewing the light
40. de 109 7 Maintaining the Dual Loop Autosampler Replacing the Peristaltic Pump Frequency When defective internal external leak solvent draining trough Tools required none Parts required Peristaltic pump includes tubing PN 5065 4445 The tubing of the peristaltic pump is pre assembled into the housing and not individually available Replacement level is the complete pump body without motor Before beginning this procedure 1 Press the clips on the upper and lower side of the peristaltic pump body together while pulling it off the Purge the peristaltic pump with non toxic motor shaft liquid e g water Remove the flush OFF open the front door 110 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 2 Pull the plastic tubings out of the pump tubing 3 Push the plastic tubings into the tubing of the new peristaltic pump upper port out to needle wash port Lower port in from wash solvent bottle 1A On completion of this procedure Re install the flush solvent bottle Start the instrument Close the front cover Agilent 1200 Series Dual Loop Autosampler User s Guide 111 7 Maintaining the Dual Loop Autosampler Exchanging the Needle Carrier Assembly Frequency When defective Tools required Hex key 2 0 mm PN 8710 2438 supplied in accessory kit Parts required Needle carrier assembly PN G1367 60010
41. ent bottle enter the new actual volume into the Actual Volume box There is no direct measurement of the bottle filling The Autosampler is calculates the actual volume by subtracting the volume of each flush cycle from the initial value of the Actual Volume variable Select Prevent analysis if level falls below and enter a threshold in the field to ensure that the pump generates a Not Ready condition if the calculated solvent bottle filling level falls below the set threshold The current run is completed but the sequence will not continue with the next analysis Select Turn off injector if running out of solvent to ensure that the injector generates an error if the calculated solvent bottle filling level is zero The error is recorded in the instrument logbook the analysis is stopped immediately and the whole system goes into an error state Adjust the length of the solvent inlet tube to the appropriate length Take off the inlet filter shorten the tubing with a cutter and replace the frit again Keeping the flush solvent tubing short minimizes the dead volume when changing flush solvents The G2258A is compatible with all well established organic solvents as well as with pure water and organic solvent water mixtures Agilent 1200 Series Dual Loop Autosampler User s Guide 63 3 Installing the Dual Loop Autosampler Configuring Wellplate Types If the plate you are using is not found on the List of Recommended Well Plates and Vi
42. er ee Agilent Technologies 91 6 Introduction to Repairing the Dual Loop Autosampler Introduction to Repairing the Dual Loop Autosampler Simple Repairs The Dual Loop Autosampler is designed for easy repair All maintenance tasks and most easy repairs such as changing rotor seal needle assembly needle seat assembly or tubings can be done from the front of the instrument without removing the module from the system stack These proceduress are described in Maintenance Procedures and Simple Repairs on page 96 When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Regularly inspect all plastic tubings of the sampler and exchange them if they are worn out or show visible signs of damage Exchanging Internal Parts Some repairs require the replacement of defective internal parts In these cases it is necessary to remove the autosampler from the stack take the covers off and disassemble the module These repairs are described in Exchanging Internal Parts in the Service Manual To prevent personal injury the power cable must be removed from the instrument before opening the top cover Do not connect the power cable to the sampler while the cover is OFF 92
43. er loop from the PEEK 7 Push the open end of the new loop capillary into the union and pull it out of the sleeve tubing sleeve tubing until it protudes at the needle arm side A l i ji 8 Push the loop tubing through the needle assembly 9 String ferrule screw new ferrule ring and new ferrule together Place the end of the buffer loop into the needle fitting 106 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 10 Tighten the ferrule screw carefully while pressing the 11 Pull excessive loop tubing out at the metering head side buffer loop firmly into the fittin
44. eral injections with various injection volumes using the same sample loop 1000 uL The peak area increases linearly until the loop is filled approximately up to 50 fill factor 0 5 which is represented by the red line This means that in order to maximize the sample recovery the maximum injection volume should not exceed 50 of the sample loop volume Figure 26 Linearity of peak area for partial loop fill 84 Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 5 Troubleshooting Overview Overview 82 Status Indicators 84 This chapter gives an Overview to Troubleshooting the Dual Loop Autosampler ee Agilent Technologies 85 5 Troubleshooting Overview Overview Status Indicators The Dual Looop Autosampler is provided with two status indicators which indicate the operational state prerun not ready run and error states of the instrument The status indicators provide a quick visual check of the operation of the Dual Loop Autosampler see Status Indicators on page 84 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see Error Messages in the Service Manual Maintenance Functions The ma
45. fer loop will destroy the wash valve Additional Configuration and Control Functions 74 Reset Injector Use this command to recover the sampler from error conditions and to move all subassemblies into a defined state When executing this command the needle arm moves into the seat of the active loop as defined in the currently loaded method the 10 port valve switches the active loop to mainpass and the plunger of the metering device goes to it s front position Needle Home Switches the 10 port valve to bypass moves the Needle arm out of the needle seat and places it into an elevated position behind the wash port This command is useful if unrestricted access to the sample tray is needed Prime Flush Pump Injector Flush Pump Dual Loop Sampler j x Prime Flush Pump M sn Close N Help Figure 21 Prime Flush Pump Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Put the end of peristaltic pump intake tubing into a bottle with needle wash solvent and place it into the solvent cabinet In case the same solvent is used to wash the outside of the needle and buffer loop and inside of the needle one bottle can be used for both Prime the peristaltic pump for a given time 1 1000 seconds to remove air and previously used solvent Since the needle wash solvent is drained through the wash port ensure proper routing of the corrugated waste tubing Use External Inje
46. ferent properties flush your syringe about 5 10 times depending on the viscosity and the volume of the inlet tube to be sure that the old solvent has been replaced quantitatively Use Propanol 2 as an intermediary for not completely miscible solvents 62 Additional Information Store your flush solvent in the solvent cabinet on top of the Agilent 1200 Series system The metering device can draw solvent only from the same height as the metering device itself or higher Always use the inlet solvent filter and clean or replace it from time to time in order to protect your metering device Similar to the Bottle Filling function of the Agilent HPLC pumps the Dual Loop Autosampler tracks the flush solvent level and stops the system before running out of solvent In ChemStation the function is accessed from the Method amp Run Control screen by pulling down the Instrument menu clicking Solvent Bottle Filling Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Actual Voke Total Volume aA pa wer 100 Mos F Prewert analysis level iols below 1010 Me I Tun ingector off running out of solvent OK _ Const tee Figure 15 Solvent Bottle Filling Enter the capacity of the solvent bottle into the Total Volume box and the actual volume into the Actual Volume box The Actual Volume field is updated by Agilent ChemStation as flush solvent is consumed When refilling the solv
47. fionw ot Upper Loop freon eo wt nek eatalied Seat Capittory Bower boop 23 wl Seat Capdluy upper boop i cane te Figure 20 DLA Configuration Screen in ChemStation Trays Shows the installed tray type The sampler is recognizing the installed tray automatically Mind the General Comments about Sample Trays on page 70 Well Plates Select the plates you intend to use from the list Front and back plate can be different types For a list of supported plates and vials see List of Recommended Vials Caps and Septa on page 124 Non listed plates can be defined using the Configure Wellplate Types function For details see Operating the Dual Loop Sampler with Agilent ChemStation on page 71 Volumes Upper Loop Volume of the upper sample loop in ul default is 5000u1 Lower Loop Volume of the upper sample loop in ul default is 50ul Seat Capillary upper loop c 24ul for the original PTFE tubing Seat Capillary lower loop c2Oul for the original PTFE tubing Agilent 1200 Series Dual Loop Autosampler User s Guide 73 4 Using the Dual Loop Autosampler CAUTION For analytical applications with limited sample volume the seat tubings can be replaced by stainless steel seat capillaries with 0 25mm ID Smaller ID seat capillaries will increase the pressure in the buffer loop during ejection Decrease the eject speed to 2000yI min or lower when using non standard seat capillaries Overpressure in the buf
48. g to avoid dead volume ca Ni 12 Push guide tubing and plastic chain into the needle 13 Slide the needle assembly into the guide on the needle assembly until they hit the hard stop carrier assembly and lock the needle assembly lever On completion of this procedure Turn the needle carrier assembly inwards Re install the side cover Re install the tray s in the tray base Turn the instrument ON Close the front door Agilent 1200 Series Dual Loop Autosampler User s Guide 107 7 Maintaining the Dual Loop Autosampler Replacing Front Seat Back Seat Assembly or Seat Tubings Frequency When one of the needle seats is leaky or worn out Typically once per year Seat tubings need to be replaced only when damaged or severely contaminated Tools required Wrench open end 1 4 5 16 inch PN 8710 0510 supplied in accessory kit flat blade screw driver with 1 4 or 6mm blade Parts required Twin seat assembly PN G2258 87102 Front seat tubing 0 5mm ID PN G2258 87308 Back seat tubing 0 8mm ID PN G2258 87309 Before beginning this procedure 1 Using a 1 4 wrench unscrew the seat tubings from port 2 oa and 6 of the 10 port valve Position the transport unit of the Dual VV
49. gure 16 Partial Loop Filling Partial loop fill without plug settings To compensate for the volume of needle seat tubing and valve groove the sampler draws the respective volume from the flush solvent bottle Next the desired sample volume is drawn from the sample vessel At the end of this step the sample plug ends at the needle tip The sampler ejects both sample and buffer plug into the needle seat Since the buffer plug has exactly the same volume as the seat tubing the complete sample volume reaches the sample loop The loop volume that was not filled with sample still contains mobile phase see Figure 16 Partial loop filling without plugs has the advantage that no flush solvent enters the column which would change column selectivity and affect the shape of fast eluting peaks Partial loop fill with plug settings This mode allows to sandwich the sample between air or solvent from any sampler location It is useful to prevent precipitation of sample or when using immiscible mobile phase solvents Typically the plug volume is calculated according tot he following equation Agilent 1200 Series Dual Loop Autosampler User s Guide 27 1 28 Introduction to the Dual Loop ALS V plug lesen dsumpi The sampler places a plug of the given volume on either side of the sample If the plug volume is smaller than calculated with the above equation the residual volume will be filled with mobile phase In all cases the trailing plug w
50. h two preinstalled sample loops of different volume allows convenient switching between preparative scale and analytical scale applications without the need for hardware modifications The sample tray can hold up to two well plates vial plates or Eppendorf tube plates in any combination For details see List of Recommended Well Plates and Vial Plates on page 123 Agilent 1200 Series Dual Loop Autosampler User s Guide 1 Introduction to the Dual Loop ALS Front View flush valve 2 a E o a km o 2 a 10 port valve optional sample thermostat Main switch with power indicator Front View of the Dual Loop Sampler Figure 1 11 Agilent 1200 Series Dual Loop Autosampler User s Guide 1 Introduction to the Dual Loop ALS Theory of Operation CAUTION Successful operation of an analytical device requires a sound understanding of the underlaying techniques This section gives an overview of the functional groups of the sampler and explains how sample is introduced into the HLPC flow path The Agilent 1200 Series Dual Loop Autosampler is a fixed loop sampler with two different sample loops As a consequence of this design the metering device also referred to as syringe is not a part of the solvent flow path from pump to HPLC column An additional flush solvent bottle is used to rinse this part of the hydraulic path after each injection In order to minimize carryover the sampler is eq
51. he CAN bus This may result in reduced system stability The LAN interface should only be installed if no other option is available Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam oF amp w N Install two pieces of corrugated waste tubing to the drain ports of the leak plane and the needle wash port 6 Re install the left side door take care of the magnet at the back Agilent 1200 Series Dual Loop Autosampler User s Guide 47 3 Installing the Dual Loop Autosampler Vial number output 7 Place the autosampler in the stack or on the bench in all horizontal position 8 Ensure the power switch at the front of the sampler is OFF 9 Connect the power cable to the power connector at the rear of the sampler 10 Connect the CAN cable to the other Agilent 1200 Series modules 11 If a Agilent ChemStation is the controller connect the computer to the Agilent 1200 Series stack by either LAN connection or GPIB 12 Connect the APG remote cable optional for non Agilent 1200 Series instruments 13 Ensure the side panel is correctly installed 14 Place the plate tray into the tray base 15 Turn ON power by pushing the button at the lower left hand side of the sampler 16 Close the front door The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start the hardware initiali
52. hing any more When defective When leaky worn or defective When defective or new board installed See Replacing the Rotor Seal of the 10 Port Valve on page 97 See Replacing the Needle Assembly on page 100 See Replacing the Buffer Loop on page 104 See Replacing Front Seat Back Seat Assembly or Seat Tubings on page 108 See Replacing the Peristaltic Pump on page 110 See Exchanging the Needle Carrier Assembly on page 112 See Replacing Seal or Piston of the Metering Unit on page 115 See Exchanging or Installing the Optional Interface BCD Board on page 120 96 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 Replacing the Rotor Seal of the 10 Port Valve Frequency When worn out if valve is leaky typically once per year Tools required 1 4 inch wrench 3 32 inch hexagonal key tweezers Parts Required rotor seal fro 10 port valve PN 0100 2415 WARNING To avoid personal injury keep fingers away from the needle area during Dual Loop Autosampler operation Before beginning this procedure 1 Disconnect all capillaries from the 10 port valve I W Disconnect the analytical column and remove toxic or corrosive solvents from the sampler by flushing with appropriate solvents e g water or IPA INI Position the trans
53. ide Index www agilent com In This Book This manual contains technical reference information about the Agilent 1200 Series Dual Loop Autosamplers The manual describes the following e Introduction to the Dual Loop Autosampler e site requirements specifications and installation e configuration and operation of the Dual Loop Autosampler e troubleshooting and test functions e maintaining the Dual Loop Autosampler e parts for maintenenance e safety information Agilent Technologies 2006 Printed in Germany 02 06 G2258 90010 pie Agilent Technologies
54. ill reach the column prior to the sample and potentially effect the peak geometry of fast eluting peaks This effect may increase with increasing plug volume relative to the sample volume reason the mobile phase composition at the column head changes dramatically with some impact on the column selectivity Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 2 Site Requirements and Specifications Site Requirements 30 Physical Specifications Dual Loop Autosampler G2258A 32 Performance Specifications of the Dual Loop Autosampler 34 Method for Carry over Test 36 This chapter describes the Site Requirements and Specifications of the Dual Loop Autosampler ee Agilent Technologies 29 2 Site Requirements and Specifications Site Requirements A suitable site environment is important to ensure optimum performance of the autosampler Power Consideration The autosampler power supply has wide ranging capability see Table 1 on page 32 Consequently there is no voltage selector in the rear of the sampler There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the sampler G2258A and the thermostat G1330B Both modules have separate power supplies and power plugs for the line connections The two modules are connected by a control cable and both are
55. ing the Needle Carrier Assembly 112 Replacing Seal or Piston of the Metering Unit 115 Exchanging or Installing the Optional Interface BCD Board 120 8 Easy Repair Parts 121 Consumables 122 List of Supported Plate Trays 122 List of Recommended Well Plates and Vial Plates 123 List of Recommended Vials Caps and Septa 124 Sample Loops for the G2258A 10 port Valve 125 Dual Loop Autosampler Accessory Kit 126 Agilent 1200 Series Dual Loop Autosampler User s Guide 7 Contents A Safety Information 129 Safety Information 130 General 130 Operation 131 Safety Symbols 132 Lithium Batteries Information 133 Radio Interference 134 Test and Measurement 134 Sound Emission 135 Manufacturer s Declaration 135 Solvent Information 136 Solvents 136 Agilent Technologies on Internet 137 8 Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 1 Introduction to the Dual Loop ALS Introduction 10 Theory of Operation 12 The Injection Sequence 16 Loop Filling Modes 26 This chapter gives an introduction to the Dual Loop Autosampler ee Agilent Technologies 1 Introduction to the Dual Loop ALS Introduction 10 The Agilent 1200 Series Dual Loop Autosampler model G2258A is optimized for applications that require rapid injection of high sample volumes Typically these are preparative HPLC separations or sample trapping and enrichment methods The dual loop concept wit
56. instrument status indicator 89 internet 137 M maintenance functions 86 missing parts 38 needle 104 needle carrier assembly 112 P park transport unit assembly 68 Agilent 1200 Series Dual Loop Autosampler User s Guide Index parts and materials 38 121 accessory kit 126 vial trays and tray base 122 performance specifications preparative scale 34 physical specifications 32 fraction collector 32 thermostat 33 power consideration 30 power cords 31 power requirements 30 power supply indicator 88 preparative needle assembly 104 Push 103 106 107 push 102 repairing the fraction collector 91 95 repairs BCD board 120 cleaning the fraction collector 93 inlet waste tubings 97 needle 104 needle carrier assembly 112 preparative needle assembly 104 simple repairs 92 96 valve to needle tubings 100 S safety information on lithium batteries 133 shipping 68 simple repairs 92 96 site requirements 30 38 specifications 32 stack configuration 44 46 rear view 44 46 status indicators 86 88 storage 32 T temperature 32 thermostat electrical connections 54 transport 68 transport unit assembly 68 transport unit self alignment 86 transporting the fraction collector 68 trays 70 troubleshooting and test functions 85 U unpacking the fraction collector 38 V valve to needle tubings 100 WwW weight 31 Agilent 1200 Series Dual Loop Autosampler User s Gu
57. intenance functions position the transport unit and needle carrier assembly in certain positions for maintenance homing or parking see Maintenance Functions in the Service Manual Transport Unit Self Alignment The transport unit alignment with the transport unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier assembly 86 Agilent 1200 Series Dual Loop Autosampler User s Guide Troubleshooting Overview 5 The transport unit self alignment is required after disassembling the system or when transport unit needle carrier assembly or MTP main board are replaced This function is in the diagnose screen of the Agilent ChemStation or the Control Module WARNING The sample transport self alignment requires one of the 2 well plate trays Part Number G2258 44502 or G2258 60001 but well plates MUST NOT be installed Agilent 1200 Series Dual Loop Autosampler User s Guide 87 5 Troubleshooting Overview Status Indicators Two status indicators are located on the front of the Dual Loop Autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Status indicator green yellow red Line power switch with green light Figure 27 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power i
58. ive humidity as described in Table 1 and Table 2 on page 33 CAUTION Do not store ship or use your Dual Loop Autosampler under conditions where temperature fluctuations may cause condensation within the Dual Loop Autosampler Condensation will damage the system electronics If your Dual Loop Autosampler was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation Table 1 Physical Specifications Dual Loop Autosampler G2258A Type Specification Comments Weight 14 kg 29 8 Ibs Dimensions 200 x 345 x 440 mm height x width x depth 8 x 13 5 x 17 inches Line voltage Line frequency Power consumption apparent power Power consumption active power 100 240 VAC 10 Wide ranging capability 50 or 60 Hz 5 200 VA Maximum 180 W Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Non condensing Up to 2000 m 6500 ft Up to 4600 m 14950 ft For storing the Dual Loop Autosampler Installation Category Il Pollution Degree 2 for indoor use only 32 Agilent 1200 Series Dual Loop Autosampler User s Guide Site Requirements and Specifications 2 Table 2 Physical Specifications Thermostat G1330B Type Specification C
59. l Comments about Sample Trays 70 Supported Trays 70 Unsupported Trays 70 Operating the Dual Loop Sampler with Agilent ChemStation 71 Navigation in Agilent ChemStation 71 Configuring the Dual Loop Sampler with ChemStation 72 Additional Configuration and Control Functions 74 Setting Method Parameters in Agilent ChemStation 76 More Method Parameters 79 Achieving the best recovery results with the Agilent 1200 Series Dual Loop AutosamplerPS 81 Introduction 81 Rinse solvent 81 Flush solvent 82 Sample draw and eject speed 83 Sample loop overfill factor for complete loop filling 83 Sample loop fill factor for partial loop fill 84 5 Troubleshooting Overview 85 Overview 86 Status Indicators 86 Agilent 1200 Series Dual Loop Autosampler User s Guide Contents Error Messages 86 Maintenance Functions 86 Transport Unit Self Alignment 86 Status Indicators 88 Power Supply Indicator 88 Instrument Status Indicator 89 6 Introduction to Repairing the Dual Loop Autosampler 91 Introduction to Repairing the Dual Loop Autosampler 92 Simple Repairs 92 Exchanging Internal Parts 92 Cleaning the Dual Loop Autosampler 93 Using the ESD Strap 93 7 Maintaining the Dual Loop Autosampler 95 Maintenance Procedures and Simple Repairs 96 Replacing the Rotor Seal of the 10 Port Valve 97 Replacing the Needle Assembly 100 Replacing the Buffer Loop 104 Replacing Front Seat Back Seat Assembly or Seat Tubings 108 Replacing the Peristaltic Pump 110 Exchang
60. l vials PN G1313 44502 Half tray for 15 x 6ml vials PN G1313 44503 These trays have larger bore holes for the vial which spoil the positioning accuracy of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Operating the Dual Loop Sampler with Agilent ChemStation Operating the Dual Loop Sampler with Agilent ChemStation Navigation in Agilent ChemStation The Agilent ChemStation chromatography software is structured into five main screens These screens can either be selected from the VIEWS menu or from the drop down list in the left upper corner of the screen Figure 18 ChemStation Views Agilent 1200 Series Dual Loop Autosampler User s Guide 71 4 Using the Dual Loop Autosampler Operating the Dual Loop Sampler with Agilent ChemStation Configuring the Dual Loop Sampler with ChemStation The Method and Run Control screen of Agilent ChemStation is the place where the hardware is configured and where method and sequence parameters are entered Pull the Instrument menu to access the DLA configuration by clicking More Injector Configuration Figure 19 Accessing the DLA configuration in ChemStation 72 Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Ingector Configuration Dual Loop Sampler x Traps vs Web Plas i Piste 1 front Irene Plate 2 back inene x Volumes wee DCD port for Lower Loop
61. lent 1200 Series Dual Loop Autosampler User s Guide 41 3 Installing the Dual Loop Autosampler Optimizing the Stack Configuration If your Dual Loop Autosampler is part of a system you can ensure optimum performance and minimum delay volume by installing the following configuration Figure 1 and Figure 2 on page 44 show the configuration recommended for the autosampler in an analytical scale system Figure 3 on page 45 and Figure 4 on page 46 show the configuration recommended for the sampler in a preparative scale system 42 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Optimizing the Stack Configuration Analytical System Solvent a Compartment Degasser Pump Autosampler UV Vis 4 Detector Fraction Column Collector Compartment rc r Figure 1 Recommended Stack Configuration Analytical System Front View NOTE For information about connecting a G1946C D LC MSD or a Non 1200 or Non UV Vis detector to the system please refer to the User s Guide for Purification High Throughput System PN G2262 90003 or to the Installation Note for the Mass Based Fraction Collection Kit PN G1968 90000 Agilent 1200 Series Dual Loop Autosampler User s Guide 43 3 Installing the Dual Loop Autosampler Optimizing the Stack Configuration Solvent Compartment Degasser e Pump GPIB or LAN sh e to LC ChemStation o g UV Vis e Efo
62. lete loop filling means that 100 of the sample loop volume will be filled with sample prior to injection Due to the laminar flow profile see Figure 15 in needle and capillaries high precision injections require drawing excessive solvent to overfill the sample loop This so called overfill factor is defined as V drawn V loop When solvent is pumped through capillaries the flow profile is not a bulk flow as one might expect but laminar flow profile This is caused by the friction of the capillary wall which slows down the nearby solvent The schematics to the left illustrate this phenomenon with the example of sample being loaded into a solvent filled loop capillary Figure 15 The Laminar Flow Profile Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 Depending on sample loop size typically overfill factors of 3 8 are necessary to obtain good peak area precision Smaller loops require higher overfill factors We recommend an overfill factor of 5 as a good starting point The G2258A Dual Loop Sampler uses a technique called Centered Complete Loop Filling When the loop is completely filled equal plugs of excessive sample are left on both ends of the loop Refer to Application Notes on page 107 for optimization of the overfill factor Partial Loop Filling Partially loop filling allows to inject any sample volume between 0 ul and the total sample loop volume Fi
63. mand 1 OCCT dot amast rio seat det 2 DRAW det amourt hom sample ma speed det cftvet Figure 23 The Injector Program Editor Agilent 1200 Series Dual Loop Autosampler User s Guide 71 4 Using the Dual Loop Autosampler CAUTION An injector program consists of one instruction per line Select the desired function from the Function drop down menu and add it by clicking Insert or Append To change a program line highlight it and hit Change Cut Copy and Paste are useful for reuse injector program lines To check and transfer the injector program to the autosampler press OK The ChemStation code validation check performs a basic syntax check Conceptional weaknesses or wrong parameter values won t be detected and remain the responsibility of the user 78 The maximum number of injector program lines is limited by the amount of free memory on the Dual Loop Sampler mainboard If needed additional memory can be allocated to extend the available space for the injector program from approximately 60 lines to 120 lines However in that case the injector program cannot be processed by he control module For details consult the ChemStation help function Plug Setting This parameter allows to enclose the sample into either plugs of air or of any desired liquid Plug Volume Defines the volume of each plug Typical plug volumes are 5 10 of the sample volume Draw Plug from This parameter offer the choice of either air plugs or
64. ngs from pump and to column last Push the waste tubings back into the receptacles in the needle seat holder Turn the instrument ON Re install the tray s in the tray base Close the front cover Perform a pressure test i WARNING Abrasive cleaning of the 10 port stator head will destroy the coating of the sealing surface and make the part unusable Agilent 1200 Series Dual Loop Autosampler User s Guide 99 7 Maintaining the Dual Loop Autosampler Replacing the Needle Assembly Frequency When worn out blocked or damaged Typically once per year Tools required Sharp knife or cutter blade Parts Required Needle assembly 2mm ferrule kit WARNING Thoroughly follow the described installation procedures to maximize the lifetime of the Dual Loop Autosampler capillary and to avoid potential spills or fraction losses Regularly inspect the capillary and exchange it if it is worn out or shows visible signs of damage Before beginning this procedure 1 Pull the lever latch of the needle assembly down to expose ae the needle fitting Position the transport unit of the Dual lt 5 Loop Autosampler in the Home Position see Maintenance Functions in the Service Manual Remove the sample tray from the tray base Z
65. nnects the metering device to the flush solvent reservoir The syringe draws a small volume of flush solvent more precisely the volume of the seat tubing of the active sample loop the volume of one groove of the 10 port valve rotor When running preparative HPLC methods with flow rates gt 20 ml min switching to bypass may generate significant back pressure and get the system into a high pressure error state It is recommended to determine the back pressure during method development and replace the analytical size sample loop by a larger one if necessary Agilent 1200 Series Dual Loop Autosampler User s Guide 17 1 Introduction to the Dual Loop ALS Drawing the Leading Buffer or Air Plug optional step Figure 6 Metering device is drawing a plug of buffer The needle arm moves to the defined location any sample tray position or just air The metering device plunger moves backwards thereby drawing the defined plug volume into needle and buffer loop Plug volumes may be defined in the range of 0 25 ul for each plug 18 Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS Drawing Sample Figure 7 Metering device drawing sample The needle lowers into the sample vessel The syringe draws sample into the buffer loop Agilent 1200 Series Dual Loop Autosampler User s Guide 1 20 Introduction to the Dual Loop ALS Washing the Needle optional step Figure 8 Needle in the
66. omments Weight 18 5 kg 40 7 Ibs Dimensions 140 x 345 x 435 mm height x width x depth 5 5 x 13 5 x 17 inches Line voltage Line frequency Power consumption active power Power consumption apparent power 100 120 or 220 240 VAC 10 50 or 60 Hz 5 210 W 260 VA Wide ranging capability Maximum Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL EN 4 40 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6500 ft Up to 4600 m 14950 ft Installation Category II Pollution Degree 2 Non condensing For storing the Dual Loop Autosampler for indoor use only Agilent 1200 Series Dual Loop Autosampler User s Guide 33 2 Site Requirements and Specifications Performance Specifications of the Dual Loop Autosampler 34 Table3 Performance Specifications Agilent 1200 Series Dual Loop Autosampler Preparative Scale Type Specification GLP features Communications Instrument control Safety features Injection modes Injection range Area precision Complete Loop Fill Area precision Partial Loop Fill Carry over Area precision Complete Loop Fill Area precision Partial Loop Fill Draw eject speed Loop size Early maintenance feedback EMF electronic records of maintenance and err
67. ons and weight see Table 1 on page 32 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the sampler is installed in a horizontal position The thermostatted Dual Loop Autosampler dimensions and weight see Table 2 on page 33 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 3 1 inches at the rear for electrical connections Ensure the sampler is installed in a horizontal position If a complete Agilent 1200 Series system is to be installed on the bench make sure that the bench is designed to carry the weight of all the modules For a complete system including the thermostatted Autosampler it is recommended to position the modules in two stacks see Recommended Stack Configuration Agilent 1200 Series Preparative LC System Make sure that in this configuration there is 25 cm 10 inches space on either side of the thermostatted Dual Loop Autosampler for the circulation of air Agilent 1200 Series Dual Loop Autosampler User s Guide 31 2 Site Requirements and Specifications Environment Your Dual Loop Sampler will work within specifications at ambient temperatures and relat
68. ors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output optional LAN communication card Agilent ChemStation software Agilent HT Purification SW Agilent G1323B Control Module Agilent G4208A Instant Pilot Leak detection and safe leak handling low voltages in maintenance areas error detection and display Partial loop filling Complete loop filling 0 1 5000 pl in 0 1 pl increments Recommended injection range 10 5000 pl Typically lt 0 5 RSD 50 pl loop overfill factor 3 or greater Typically lt 1 0 RSD 5000 pl loop 1000p injection volume draw eject speed 20 ml min lt 0 1 with rinse factor gt 3 see test method listing Typically lt 0 5 RSD 50 pl loop overfill factor 3 or greater Typically lt 1 0 RSD 5000 pl loop 1000 pl injection volume draw eject speed 20 ml min max 50 ml min selectable in 1 pl min steps max loop size 5 ml selectable range 5 pl 50000 pl Agilent 1200 Series Dual Loop Autosampler User s Guide Site Requirements and Specifications 2 Table3 Performance Specifications Agilent 1200 Series Dual Loop Autosampler Preparative Scale continued Type Specification Sample viscosity 0 2 5 cp range Injection volume Selectable range 0 50000 pl in 0 1 pl steps Recommended range 10 ul 5000 pl in 1 pl steps Temperature Temperature range of sample compartment 4 40 deg C con
69. osampler User s Guide 23 1 Introduction to the Dual Loop ALS Drawing Flush Solvent Figure 12 Metering device drawing flush solvent The flush valve connects the syringe to the flush solvent bottle The metering device draws the flush volume 2 X Vpjug Vsample 24 Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 Washing the Sampling Path ay OV Figure 13 Flushing sampling flow path with flush buffer Buffer loop needle needle seat seat capillary and valve groove are purged with flush solvent The last two steps can be repeated multiple times to ensure lowest carryover 3 5 wash cycles are sufficient for most samples Agilent 1200 Series Dual Loop Autosampler User s Guide 25 1 26 Introduction to the Dual Loop ALS Loop Filling Modes Fixed loop autosamplers usually purge the loop with sample before switching it into the flow path and flushing the content onto the column As a consequence changing the injection volume requires the installation of a loop of different size Volumes of loops of identical size vary up to 65 depending on loop size and material The Agilent 1200 Series Dual Loop Autosampler overcomes this limitation with a 10 port valve and two loops of different sizes Furthermore it offers different loop filling modes that allow the injection of virtually any desired volume Complete Loop Filling i Figure 14 Complete Loop Filling Comp
70. ovide also the latest firmware of the Agilent 1200 series modules for download Agilent 1200 Series Dual Loop Autosampler User s Guide 137 A Safety Information Agilent Technologies on Internet 138 Agilent 1200 Series Dual Loop Autosampler User s Guide A accessory kit contents 41 Agilent on internet 137 air circulation 31 battery safety information 133 BCD board 120 bench space 31 C cable connecting APG remote 44 46 connecting CAN 44 46 connecting GPIB 44 46 connecting LAN 44 46 connecting the ChemStation 44 46 connecting the power 44 46 cable connections 53 capillaries 55 capillary guiding assembly exchange 110 capillary tip alignment 102 103 106 107 cleaning the fraction collector 93 collection order 73 condensation 32 configuration collection order 73 trays 70 D damaged packaging 38 delivery checklist 38 E electrical connections thermostat 54 environment 30 32 38 error messages 86 F failure 86 flow connections 55 fuses 30 inlet waste tubings 97 installing the autosampler 47 interface cables 47 power cable 47 safety 47 installing the fraction collector 47 flow connections 55 interface cables 47 power cable 47 safety 47 installing the thermostatted autosampler interface cables 50 power cable 50 preparation 50 safety 47 50 installing the thermostatted fraction collector interface cables 53 power cable 53 safety 47 53
71. ovided with terminal for protective earthing and has been manufactured and tested according to international safety standards WARNING If you are using flammable solvents remove the well plates from the tray when you turn OFF the sampler You avoid the risk of building explosive gas mixtures in the tray compartment WARNING If you are using flammable solvents cover the well plates with pre slit closing mats to avoid the risk of building explosive gas mixtures WARNING After a leak in the sampler make sure the leak plane is cleaned and dry 130 Agilent 1200 Series Dual Loop Autosampler User s Guide Safety Information A Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaire
72. plugs of any solvent from a given vessel position Needle Wash Needle wash minimizes carryover by dipping the needle into either the flush port or any sample location The flush port is fed by a peristaltic pump Needle flush solvent can either be taken from the flush solvent bottle or a different vessel Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Rinse Complementing the Wash command Rinse allows to flush buffer loop and the inside of the needle Rinse Volume Defines the relative rinse volume for buffer loop and needle according to the following formula V flush 2V plug V sample V seatcap V valve with V flush Flush volume V plug Volume of buffer or air plug V sample Volume of injected sample V seatcap Volume of installed seat tubing V valve Volume of valve stator ports and rotor groove 4 9 pl Rinse Draw Speed Rinse Eject Speed Speed at which the sampler draws and ejects flush solvent As small volume deviations are not critical higher speeds as when drawing sample can be used Limits are 350 ul min to 50 000 ul min Default is 50 000u1 min More Method Parameters By clicking More the windows expands and auxiliary parameters become visible Draw Speed Defines at what flow rate liquid is sucked into the buffer loop Valid entries are 350 ul min 50 000 ul min Eject Speed Defines at what flow rate the content of the buffer loop is ejected
73. port unit of the Dual Loop Autosampler in the Home Position see Maintenance Functions in the Service Manual Remove the trays from the tray base Turn the instrument OFF Agilent 1200 Series Dual Loop Autosampler User s Guide 97 7 Maintaining the Dual Loop Autosampler 2 Using the 3 32 inch hexagonal key remove 5 hex screws and take the stator head of the valve off 3 Pull the old rotor seal out It may be necessary to grab it 4 Push the new seal onto the three alignment pins of the valve rotor The pins are not equidistant and will only allow to fit the seal in the correct orientation Y Lg Pe 5 Inspect the sealing surface of the stator head Replace the stator head if scratches are visible which cannot be removed with a soft piece of cloth wo 98 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 7 6 Remount the stator head The alignment pin of the valve 7 Reinstall the 5 hex screws Tighten them over cross and as ensures that the stator can only be mounted in the correct equally as possible This ensures that the stator face is position parallel to the rotor seal and does not leak when the valve 7 is operated 8 Reconnect the tubings loops and capillaries to the valve On completion of this procedure Start with the shortest fittings and connect the tubi
74. ribes the Consumables and Accessories of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide A Safety Information This chapter gives Safety Information for the use of the Dual Loop Autosampler Agilent 1200 Series Dual Loop Autosampler User s Guide Contents Introduction tothe Dual Loop ALS 9 Introduction 10 Front View 11 Theory of Operation 12 The Injection Sequence 16 Loop Filling Modes 26 Site Requirements and Specifications 29 Site Requirements 30 Power Consideration 30 Power Cords 31 Bench Space 31 Environment 32 Performance Specifications of the Dual Loop Autosampler Method for Carry over Test 36 Installing the Dual Loop Autosampler 37 Unpacking the Dual Loop Autosampler 38 Damaged Packaging 38 Delivery Checklist 38 Accessory Kits 39 Optimizing the Stack Configuration 42 Analytical System 43 Preparative System 45 Installation 47 Installing the Dual Loop Autosampler 47 Installing a Thermostatted Dual Loop Autosampler 50 Flow Connections to the Dual Loop Autosampler 55 Agilent 1200 Series Dual Loop Autosampler User s Guide 34 Contents Contents Flushing the Metering Device 58 Initial Priming by Backflushing with External Pump 58 Purging the Syringe 59 Flushing the Syringe 60 Configuring Wellplate Types 64 Special Transport Unit Positions 68 Transporting the Dual Loop Autosampler 68 Maintenance Positions 68 Using the Dual Loop Autosampler 69 Genera
75. s ON 88 Agilent 1200 Series Dual Loop Autosampler User s Guide Troubleshooting Overview 5 Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions e When the status indicator is OFF and power switch light is on the instrument is in a prerun condition and is ready to begin an analysis e A green status indicator indicates the instrument is performing an analysis run mode e A yellow status indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front door not closed or while a self test procedure is running e An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis Agilent 1200 Series Dual Loop Autosampler User s Guide 89 5 Troubleshooting Overview Status Indicators 90 Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 6 Introduction to Repairing the Dual Loop s Autosampler Cleaning the Dual Loop Autosampler 93 Using the ESD Strap 93 This chapter gives an Introduction to Repairing the Dual Loop Autosampl
76. sation process At the end of this process the status LED should be green CAN cable to previous module t optional board L r G Nh p RELAY CONTACTS i ca Connection to C C lee g Th e rmostat THERMO REMOTE CAN DC OUT CONFIG pj SSeSsSSS5S ANN a wen SS SSS c HNN AO CAN cable to next module Figure 5 Cable Connections 48 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The sampler is turned OFF when the line power switch is protruding and the green light is OFF WARNING To disconnect the sampler from the line unplug the power cord The power will supply still uses some power even switch at the front panel is turned OFF Agilent 1200 Series Dual Loop Autosampler User s Guide 49 3 Installing the Dual Loop Autosampler Installing a Thermostatted Dual Loop Autosampler Preparation Locate bench space Provide power connections Unpack the sampler and the thermostat Parts required Sampler and thermo
77. sing with a inaccurate well definitions may cause the sampler to slam the needle into the bottom of the sample vessel causing damage to the needle assembly 80 Agilent 1200 Series Dual Loop Autosampler User s Guide Using the Dual Loop Autosampler 4 Achieving the best recovery results with the Agilent 1200 Series Dual Loop Autosampler PS CAUTION Important Information Please read before using the instrument Introduction The injection principle of the dual loop autosampler PS is different to that of other Agilent 1200 Series autosamplers It is a fixed loop push through design as shown in Figure 24 Since the sample has to be drawn into a buffer loop and then transferred into the injection loop several items have to be considered to achieve best recovery results Figure 24 Injection principle dual loop autosampler Rinse solvent e The rinse solvent is used to rinse the injection port the seat capillary and the injection valve after sample injection Agilent 1200 Series Dual Loop Autosampler User s Guide 81 4 Using the Dual Loop Autosampler e To avoid precipitation problems use a solvent in which the sample is readily soluble For reversed phase chromatography this could be acetonitrile methanol or DMSO acetonitrile 50 50 v v for example pure DMSO is not recommended due to its high viscosity see Sample draw and eject speed e We strongly recommend to use the Rinse function subsequently to e
78. stat Power cord for the other cables see below and Cable Overview inthe Service Manual ChemStation and or Control Module G1323B and or Instant Pilot G4208A 1 Place the thermostat onto the bench 2 Remove the front cover and route the condensation drain tube to the waste bottle WARNING Make sure that the condensation drain tube is always above the liquid level in the vessel If the tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the instrument This may damage the instruments electronics 50 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Condensation drain tube Waste bottle N j Figure 6 Condensation leak outlet 3 Install optional BCD or LAN interface boards in Agilent 1200 Series detector For details see Firmware Description in the Service Manual CAUTION Always use the detector as access point for the data system If another module is used to connect the PC all data especially 3D data from the detector have to be routed via the CAN bus This may result in reduced system stability The LAN interface should only be installed if no other option is available Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam uy oO ol A Install two pieces of corrugated wa
79. ste tubing to the drain ports of the leak plane and the needle wash port 8 Re install the left side door take care of the magnet at the back 9 Place the sampler on top of the thermostat Make sure that the sampler is correctly engaged in the thermostat locks 10 Install the connection cable between sampler and thermostat Agilent 1200 Series Dual Loop Autosampler User s Guide 51 3 Installing the Dual Loop Autosampler Do not disconnect or reconnect the Dual Loop Autosampler to thermostat cable when the power cords are connected to either of the two modules This will damage the electronics of the modules 52 11 Remove the tray and the plastic cover from the tray base place the air channel adapter into the sampler tray base Make sure the adapter is fully seated 12 Place the plate tray into the tray base 13 Turn ON power by pushing the button at the lower left hand side of the Dual Loop Autosampler 14 The exhaust fan will turn ON and remove potential solvent vapor from the inside of the instrument After 2 minutes close the front door Then the Dual Loop Autosampler will start the hardware initialization process At the end of this process the status LED should be green Agilent 1200 Series Dual Loop Autosampler User s Guide onnection to Thermostat Remote Connector RS232C Installing the Dual Loop Autosampler 3 CAN cable to previous module
80. t RF tag right Bolt the metering head assembly together with the two 12 Insert the two hex screws into the metering head 13 Position the solenoid valve and the buffer loop extension assembly and screw it onto the metering unit above the metering head assembly and fix them with the Ensure not to catch the solenoid cable two 3mm Pozidrive screws Reinstall the purge plug 118 Agilent 1200 Series Dual Loop Autosampler User s Guide Maintaining the Dual Loop Autosampler 14 Reconnect the buffer loop to the PEEK fitting of the buffer On completion of this procedure loop extension and the solenoid tubing to the metering head Start the instrument 7 P Close the front cover Prime the metering unit as described in Flushing the Metering Device 58 Agilent 1200 Series Dual Loop Autosampler User s Guide 119 7 Maintaining the Dual Loop Autosampler Exchanging or Installing the Optional Interface BCD Board Frequency At installation when defective or for all repairs inside the Dual Loop Autosampler Tools required Flat head screwdriver Parts required Optional interface board CAUTION The interface board is sensitive to electrostatic discharge Always use the ESD strap when handling electronic boards Switch OFF the Dual Loop Autosampler
81. the Rotor Seal of the 10 Port Valve 97 Replacing the Needle Assembly 100 Replacing Front Seat Back Seat Assembly or Seat Tubings 108 Replacing the Peristaltic Pump 110 Exchanging the Needle Carrier Assembly 112 Replacing Seal or Piston of the Metering Unit 115 Exchanging or Installing the Optional Interface BCD Board 120 This chapter describes the Maintenance and Simple Repairs of the Dual Loop Autosampler ait Agilent Technologies 95 7 Maintaining the Dual Loop Autosampler Maintenance Procedures and Simple Repairs The procedures described in this section can be done without removing the Dual Loop Autosampler from the stack Table 7 Maintenance Procedures Procedure Typical Frequency Notes Replacing the rotor seal of the 10 port valve Replacing the needle assembly Exchanging the buffer loop tubing Replacing the front seat back seat assembly or seat tubings Replacing peristaltic pump Replacing the needle carrier assembly Exchanging the seal or piston of the metering unit Exchanging or installing the optional BCD board When worn out if valve is leaky typically once per year When worn out blocked or damaged Typically once per year When the buffer loop tubing is damaged or too short Typically replaced with every 3rd needle assembly When worn out leaky or showing visual signs of damage typically once per year When defective internal external leak valve not switc
82. tive PumpA Column Organizer GPIB or LAN E to ChemStation P reparative TERS Pump B of CAN Bus UV Vis OE nee Cable Detector 5 mn H a a L C Autosampler Fraction jbo oS T mom ome a Collector A _ m E a OC Figure 4 Recommended Stack Configuration Preparative System Rear View If a G1330B ALS thermostat is part of the system it must always be installed underneath NOTE ase the Dual Loop Autosampler see on page 53 46 Agilent 1200 Series Dual Loop Autosampler User s Guide Installing the Dual Loop Autosampler 3 Installation Installing the Dual Loop Autosampler Preparation Locate bench space Provide power connections Unpack the sampler Parts required Sampler Power cord for the other cables see below and Cable Overview in the Service Manual Chemstation rev A 10 02 or higher and or Control Module G1323B rev B 03 21 or higher and or Instant Pilot G4208A WARNING To avoid personal injury keep fingers away from the needle area during Dual Loop Autosampler operation Do not attempt to insert or remove a vial or a plate when the needle is positioned 1 Install optional BCD or LAN interface boards in Agilent 1200 Series detector For details see Firmware Description in the Service Manual CAUTION Always use the detector as access point for the data system If another module is used to connect the PC all data especially 3D data from the detector have to be routed via t
83. trolled version with G1330B Sample capacity 2 x well plates MTP additional 10 x 2 ml vials Supported sample Any combination of 2 of the following plates plates 96 well plate shallow deep conical 54 x 2 ml vial plate 15 x 6 ml vial plate 27 x Eppendorf plate for Eppendorf tubes of 0 5 ml 1 5 ml or 2 0ml Maximum plate height with plate tray G2258 60001 50 mm Vial septa and closing mats must be pre slit Agilent 1200 Series Dual Loop Autosampler User s Guide 35 2 36 Site Requirements and Specifications Method for Carry over Test Table4 Carry over Test Method Parameter Setting Column Zorbax SB C18 21 2 x 50 mm 5 um Solvents A water B ACN Gradient 0 min 5 B 5 0 min 95 B 5 5 min 95 B Flow rate 20 ml min Stop time 5 5 min Post time 2 min Inj volume 250 ul partial loop filling 500 pl loop Column temperature DAD Sample ambient 254 nm 8 ref 360nm 100 Preparative flow cell 0 3 mm path length Caffeine Primidone Mandelic acid Benzylester Biphenyl 5 mg each on column Agilent 1200 Series Dual Loop Autosampler User s Guide Agilent 1200 Series Dual Loop Autosampler User s Guide 3 Installing the Dual Loop Autosampler Unpacking the Dual Loop Autosampler 38 Optimizing the Stack Configuration 42 Installation 47 Flushing the Metering Device 58 Configuring Wellplate Types 64 Special Transport Unit Positions 68 This chapter describes the Installation of the Dual
84. turned on by the sampler module The thermostat power supply has two externally accessible fuses To disconnect the Dual Loop Autosampler from line power unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned OFF To disconnect the thermostatted Dual Loop Autosampler from line power unplug the power cord from the autosampler and the thermostat The power supplies still use some power even if the power switch ON the front panel is turned OFF Please make sure that it is always possible to access the power plug Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified 30 Agilent 1200 Series Dual Loop Autosampler User s Guide Site Requirements and Specifications 2 Power Cords Your Dual Loop Autosampler is delivered with a power cord which matches the wall socket of your particular country or region The plug on the power cord which connects to the rear of the instrument is identical for all types of power cord WARNING Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the power cord designed for your region WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The Dual Loop Autosampler dimensi
85. uipped with a needle wash function which dips the needle into a wash vial or the wash port The wash port is fed by a peristaltic pump and can be operated with most common HPLC solvents The needle seat assembly incorporates two independent needle seats The front needle seat is used to fill the lower sample loop whereas the back seat is connected to the upper sample loop The needle is fitted with a second shorter needle that vents the vial to the outside and guarantees rapid pressure equilibration between vial and ambient Due to the strong force that is needed to penetrate the vial septum with this needle assembly Agilent recommends to use pre slit septa and closing mats only Non pre slit septa may get pushed into the vials or cause needle movement errors 12 Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 eory of Uperation al Wide ID needle for drawing from vessels or ejecting into needle seats or other positions Pressure equilibration needle welded to the main needle Lever for closing the leak drain seal Sealing rim Figure 2 Dual Loop Autosampler Twin Needle Assembly Agilent 1200 Series Dual Loop Autosampler User s Guide 13 Introduction to the Dual Loop ALS Theory of Operation Buffer Loop Needle Solenoid Valve Needle Metering Twinseat Device Upper Loop Fram Sa Flush Solven Injection Valve From Pump
86. umert Method Sequence View Abert Help Method and Set up Injector a aws e E Suno a Jab we AA Figure 11 Accessing the Purge Syringe Commend It is suggested to use this function at least before starting a sequence to ensure that no gas bubbles have formed inside the metering unit When changing solvents it may be necessary to use intermediate flushing steps with Isopropanol or water CAUTION Air in the metering device results in unpredictable injection volumes and large peak area standard deviation It is essential to remove any gas from the metering head the solenoid valve and all tubing before an analysis is started Agilent 1200 Series Dual Loop Autosampler User s Guide 59 3 60 Installing the Dual Loop Autosampler 1 Start purging the sampler from the Instrument menu of Agilent ChemStation see Figure 11 or from Views gt System gt DL Sampler gt Purge gt Syringe on the handheld controller until the flush solvent has reached the inlet tubing to the metering head 2 Continue purging for another 5 to 10 times to ensure that metering head is free of gas bubbles 3 If applicable Change the solvent as needed and flush 10 times with new solvent Flushing the Syringe Reasons to flush the Syringe The metering device syringe of the Dual Loop Autosampler is a dead end part of the system see Figure 12 Figure 12 Schematic of needle buffer loop wash valve and syringe Gas
87. wash port The needle is lowered into the wash port The peristaltic pump delivers needle wash solvent for the defined wash time Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 Drawing the Trailing Buffer or Air Plug optional step Figure 9 Metering device is drawing a plug of buffer The needle arm moves to the defined location any sample tray position or just air The syringe is drawing a buffer or air plug of the same volume as the leading plug Agilent 1200 Series Dual Loop Autosampler User s Guide 21 1 Introduction to the Dual Loop ALS Loading the Sample Loop Figure 10 metering device loading the working loop The needle moves into the seat of the working loop rear seat upper loop front seat lower loop The plunger ejects both optional buffer plugs and the sample into the working loop The dead volume of seat tubing and valve groove are compensated for by the flush solvent plug drawn in step 1 In case the working loop is only loaded partially the remaining volume is still filled with run solvent 22 Agilent 1200 Series Dual Loop Autosampler User s Guide Introduction to the Dual Loop ALS 1 Injecting Figure 11 10 port valve switching to mainpass The 10 port valve switches to mainpass and the content of the sample loop is flushed out towards the column The sample loop stays in the flow path until the end of the run Agilent 1200 Series Dual Loop Aut

Agilent 1200 Series Dual Loop Autosampler User Guide(1)

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