Agilent HPLC-Chip: G4240-61002 Infusion
Contents
1. 1 of the u WPS valve 3 Disconnect capillary C 75 um blue from the Chip Cube valve stator and retract it into the guide tube to avoid damage to the end 4 Disconnect capillary B 25 um yellow on the Chip Cube side and reconnect it to port 3 of the Chip Cube valve stator 5 The waste capillary D can remain connected to whatever port it is connected Your system is now ready to perform flow injection simply by inserting the G4240 61002 Infusion Chip in to the Chip Cube To revert to normal operation with analytical HPLC chips perform the above steps in reverse order Flow rate When performing infusion with a syringe switch both nano pump and capillary pump to normal mode and set the flow rates to 0 Set the syringe pump to a flow rate that gives you the best signal on the mass spectrometer A We 0 Part Number G4240 90202 Edition 12 200 Printed in Germany good flow rate to start with is 18 uL h equals 0 3 L min For flow injection switch the capillary pump to normal mode and set the flow rate to 0 The flow rate of the nano pump should be set to 0 3 uL min and can be adjusted to give the best signal on the mass spectrometer In both cases the flow rate should not exceed 1uL min with recommended values around 0 3 to 0 6 L min For infusion manually start the run on the mass spectrometer Please refer to your Agilent mass spectrometer operating manual for information on how to use and set up manual data acquisition F2. G 6 amp e e 2 0 ee eo 99 bed e E General Description The 64240 62002 HPLC Chip is designed to allow easy flow injection or direct infusion of the sample into the mass spectrometer For use in flow injection mode reconfiguration of some capillary connections is required The chip is connected to either the micro well plate autosampler flow injection or an external syringe pump infusion using stator valve port 3 of the HPLC Chip cube and a 75 um capillary and connection fittings Once the capillary tubing connection is established It is possible to perform sample infusion or flow injection at any time by simply inserting the Infusion Chip in the Chip Cube Safety Considerations All points of connection in liquid chromatographic systems are potential sources of leaks Users of liquid chromatographic equipment should be aware of the toxicity or flammability of their mobile phases Operational Guidelines Inspect the HPLC Chip immediately upon its arrival If there are any signs of damage notify your local Agilent representative at once Record the HPLC Chip type and serial number purchase date and operating limits Keep a record of chip usage This record will be invaluable in diagnosing HPLC Chip problems Agilent HPLC Chip G4240 61002 Infusion Care amp Use data sheet for the Infusion Chip Scratches and other markings may be visible in the rotor area of the HPLC Chip Do not attempt to re
3. move them This is normal and occurs during the manufacturing final test The appearance of additional scratches and markings in the rotor area following use of the HPLC Chip is also normal and does not have any impact on chip performance Maximum operating pressure for the HPLC Chip is 150 bar 2175 psi Avoid use of mobile phases below pH 0 8 or above pH 8 0 Solvents with pH gt 8 5 must not be used as they dissolve the quartz capillaries in the nano LC system and Chip Cube Avoid touching the exposed polyimide surfaces of the HPLC Chip Do not manually extend the HPLC Chip tip outside of the chip holder as this may result in tip damage and poor spray performance When not in use always store the HPLC Chip in a storage box to avoid dust or other foreign particle contamination Special Considerations for use of the Infusion Chip The Agilent HPLC Chip is specifically designed for use with the Agilent G4240A HPLC Chip MS Cube interface Attempting to use the HPLC Chip with other devices can damage the chip General use of HPLC Chips with the G4240A HPLC Chip MS Cube is described in the Chip Cube user manual P N G4240 90000 Descriptions for connection and precautions described here refer to this system Special connection and operating requirements for the G4240 61002 Infusion Chip are described in this data sheet only Agilent Technologies For more information on our products visit the Agilent Technologies home page on the wo
4. or flow injection data acquisition will start automatically when the sample is injected by the micro well plate autosampler Column Pressure The very narrow D capillaries used in the system for connections to the syringe pump capillary pump and micro well plate sampler may occasionally plug despite the very smooth inner surface of such capillaries It is good practice and strongly recommended to monitor and log the HPLC Chip pressure regularly under standard conditions In case there is a deviation of more than 10 from previously logged values one of the transfer capillaries may be plugged In such case proceed as described in the chip care section Infusion Chip Care If solvent flow through the Infusion chip appears to be restricted high pressure check to see that solvent flow is unobstructed up to the HPLC Chip inlet connection capillaries If replacing the connecting capillaries fails to restore flow through the chip the chip most likely requires replacement In order to protect your Infusion chip from overpressure conditions set the maximum pressure limit of the nano pump to 150 bar Infusion Chip Storage The HPLC Chip may be safely stored in most mobile phases Agilent recommends to use a mixture of 50 acetonitrile and 50 water Always flush out the Infusion chip with solvent and store It in a storage box to avoid dust or other foreign particle contamination HPLC Chip radio frequency identification tag The HPLC Chi
5. p contains a radio frequency RF identification ID tag in the chip handle The RF ID tag contains information on the HPLC Chip layout The information on the RF ID tag is automatically downloaded and updated by the system software from the HPLC Chip Cube interface Information stored on the chip RF ID tag Product number Serial number Revision Max Pressure Limit Number of injections Time stamp of first usage Time stamp of last usage Total time in operation User comment field Agilent Technologies Inc 2005 Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany
6. rld wide web at http www agilent com chem supplies For Technical Support in the US and Canada call 1 800 227 9770 or call your local Agilent sales office Special Operational Guidelines for the Infusion Chip Capillary Connections Infusion with Syringe Pump Plumbing Diagram for Infusion with Syringe Pump capillary svii nano yringe pump pump flow sensor flow sensor A 5 4 MD fe 1 2 u WPS valve chip cube valve Fig 1 Sample infusion with the syringe pump is similar to calibrant infusion and does not require changes to the standard plumbing shown in fig 1 The sample is introduced via capillary C 75 um blue into port 3 of the Chip Cube valve stator which connects directly to the emitter tip If it is not installed install this capillary now For direct infusion of the sample fill a 100 uL syringe with your sample and infuse at a flow rate of 18 L h Make sure to flush syringe capillary C and Infusion chip thoroughly with an appropriate solvent after use e g 50 50 ACN water Flow Injection with Well Plate Sampler Plumbing Diagram for Infusion with Auto Sampler capillary pump flow sensor syringe u WPS valve chip cube valve Fig 2 For flow injection make the following modifications according to fig 2 1 Disconnect capillary E 50 um green from port 1 of the y WPS valve 2 Disconnect capillary A 15 um orange from port 2 of the Chip Cube valve stator and reconnect it to port
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