Agilent 1260 Infinity Standard Degasser User Manual
Contents
1. p n 01046 60201 Pin Universal Pin Agilent Signal Name Active module TTL 1 White Digital ground A O 1 oo 2 Brown Prepare run Low o0 oo og 3 Gray Start Low KEY coal DE D5 4 Blue Shut down Low om 5 Pink Not connected oOo 0 6 Yellow Power on High Ss O 15 7 Red Ready High 8 Green Stop Low 9 Black Start request Low Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent vacuum degasser The other end is for general purpose 68 1260 Standard Degasser User Manual Agilent Vacuum Degasser to general purposes Identifying Cables p n G1322 81600 Color Pin Agilent Signal Name 1100 a White 1 Ground Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5VIN Pink 6 Vent 1260 Standard Degasser User Manual 69 8 Identifying Cables Cable Overview 70 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 9 Appendix General Safety Information 72 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 75 Radio Interference 76 Sound Emission 77 Agilent Technologies on Internet 78 ee Agilent Technologies 71 9 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety stand2. Notices Agilent Technologies Inc 2006 2008 2010 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61322 90012 Edition 06 10 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with war
3. EN 4 Tubular plastic oN membranes CY QY Solvent oon 9 Vacuum container LI Figure 1 Overview only one of the four solvent channels is shown 6 1260 Standard Degasser User Manual Introduction 1 How the Electronics Work The vacuum degasser has two different normal operation modes and a continuous mode In operation mode 1 the vacuum degasser works around a defined set point 115 Torr Due to environmental conditions it is possible that the vacuum degasser cannot reach the pre defined set point Under this condition the operation mode 2 becomes active and the vacuum pump is activated in defined time intervals vacuum level 115 to 190 Torr In case of a malfunction of the vacuum degasser vacuum level above 190 Torr the instrument is turned into the error mode Pressure Ambient Error Limit Operation Mode 1 time Figure 2 Operation Modes of the Vacuum Degasser The main function of the vacuum degasser control assembly is to control the vacuum pump and to check the vacuum in the vacuum container The power section of the control assembly comprises a switching power supply that generates 24 V from line voltage The 24 V is used to drive the vacuum pump and the solenoid valve The electronic control circuit uses 12 V which is generated from the 24 V The pressure sensor is connected to the vacuum chamber and checks for the correct vacuum in the system 1260 Standard Dega
4. Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed CAUTION Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 12 1260 Standard Degasser User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can l
5. Degasser User Manual 49 6 Maintenance Introduction to Maintenance 50 Simple Repairs Maintenance The standard degasser is designed for easy repair The most frequent repairs such as exchanging power fuses and status light pipes can be performed by the user but require opening the main cover of the standard degasser These repairs are described in this chapter Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD strap supplied in the standard accessory kit when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 11 Using the ESD Strap 1260 Standard Degasser User Manual Maintenance 6 Cleaning the Instrument Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings The vacuum degasser case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can
6. Degasser User Manual 73 9 Appendix Safety Symbols Table5 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up ii alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 74 1260 Standard Degasser User Manual Appendix 9 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requi
7. Identifying Cables 8 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Standard Degasser User Manual 65 8 Identifying Cables Remote Cables OOO ofecc eco One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3394 Pin Agilent Signal Name Active module TTL a 9 1 White Digital ground NC 2 Brown Prepare run Low 80 3 3 Gray Start Low z NC 4 Blue Shut down Low a NC
8. Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 78 1260 Standard Degasser User Manual Index Index A condensation 14 on sound emission 77 Agilent control circuit 6 installation oninternet 78 cover 60 bench space 14 algae growth 38 internal leak 46 ambient operating temperature 15 D mengt l 78 ambient non operating temperature 15 dead volume 20 introduction to the degasser 6 defect on arrival 18 20 L line frequency 15 line voltage 15 16 16 APG remote connector 24 AUX output 24 auxiliary cable 68 analog output delay volume delivery checklist 19 dimensions 15 E M B electronic waste 75 main cover assembling 55 14 electronics how does itwork 7 bench space materials in contact with solvent 16 busy condition 46 maximum flow rate 16 error condition 46 maximum sensitivity 36 C exchanging mounting tool 27 27 cable status light pipe 58 auxiliary 19 68 F N CAN 24 non operating altitude 15 power 19 52 flow connections 26 26 non operating temperature 15 remote 19 66 flowrate 16 number of channels 16 cables frequency range 15 analog 64 front of the vacuum degasser 25 0 BCD 64 Aan es fuse 56 operating altitude 15 ewomaleanae ae H operating temperature 15 LAN 65 operational hints 30 aveniew Ot highes
9. drip into the vacuum degasser 1260 Standard Degasser User Manual 51 6 Maintenance Removing and Refitting the Top Cover Tools required Screwdriver Pozidriv 1 Preparations Switch OFF the degasser at the main power switch Disconnect the power cable and remote cable Disconnect all solvent tubes from the ports of the degasser Remove solvent cabinet from the degasser Remove degasser from the stack WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Remove the front panel 2 Unclip the clips on the top cover Clip ES 52 1260 Standard Degasser User Manual Maintenance 6 Introduction to Maintenance 3 Lift the cover away 4 Unscrew the screws on the top plate and remove the plate by lifting its back and then sliding the plate to the front Screws 5 Place the metal cover onto the housing Lower the front of the plate first then slide pan
10. is also recommended for an isocratic or a high pressure mixing pump if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires optimum injection precision or if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min if your sample or detection is sensitive to dissolved oxygen in the mobile phase degradation Generally a degasser should be used when negative effects due to dissolved gas in the mobile phase exceed the limits that are acceptable for the user Negative effects that can be caused by dissolved gas are Unstable flow due to unstable pumping conditions This may result in a high ripple unstable pressure at constant flow and with constant mobile phase composition or high standard deviations of peak retention times and peak areas especially at low flow rates e Baseline noise on detectors that are sensitive to changes in the refractive index e g RI detector or UV detector in the low UV range both at maximum sensitivity e Sample degradation Fluorescence quenching due to dissolved oxygen Baseline drift in electrochemical detectors due to dissolved oxygen especially in reduction mode 1260 Standard Degasser User Manual Using the Vacuum Degasser 4 Solvent Information Always filter solvents through 0 4 um filters small particles can permanently block the capillaries Avoid the use of the following ste
11. mV are exceeded the vacuum pump will be started to keep the vacuum with in its working limit The amplifier and comparator circuit also activates the timer 3 when the vacuum in the vacuum chambers is below operation mode 1 The timer 3 is reset when operation mode 1 is reached within a maximum of 8 minutes If operation mode 1 is not reached and the time 8 minutes of timer 3 elapsed the timer mode operation mode 2 is activated In this mode the vacuum pump is automatically turned on every 2 minutes for a time frame of 30 seconds Timers 1 and 2 are activated as described earlier The error monitor continuously checks the error limit of the degasser 190 Torr If the error limit is exceeded for example leak in chamber the error timer is activated and the yellow status indicator lamp is turned on The vacuum pump is turned on continuously If the vacuum pump cannot reach either of its two operation modes within 8 minutes error timer limit the error driver is activated The error driver will deactivate the vacuum pump and solenoid valve The status lamp turns red and the error output on the remote connector will be activated 1260 Standard Degasser User Manual Introduction 1 The error output at the remote connector provides a contact closure potential free open collector maximum load 35 V DC 50 mA as long as the error condition is active The error condition is set closed when the status light shows the error condition red
12. the vacuum pump will be significantly reduced When to use Continuous Mode In continuous mode the vacuum pump runs continuously thus generating the highest degassing efficiency of the degasser This mode is only recommended for very sensitive applications e g RI detection Error Mode The error level for the standard degasser is 190 Torr approximately 800 mV This level cannot be achieved when there is a failure in the degasser for example leaks etc When the error level is exceeded the yellow status indicator lamp is turned on and the vacuum pump runs continuously If the degasser cannot reach one of the normal operation modes within 8 min the status indicator turns red and the vacuum pump is turned OFF 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 6 J Troubleshooting and Diagnostics Overview of the Degasser s Indicators 44 Status Indicators 45 Power Supply Lamp 45 Instrument Status Indicator 46 ee Agilent Technologies 43 5 Troubleshooting and Diagnostics Overview of the Degasser s Indicators 44 Status Indicators The standard degasser is provided with two status indicators which indicate the operational state ready busy and error states of the standard degasser The status indicators provide a quick visual check of the operation of the standard degasser see Status Indicators on page 45 Hardware Symptoms A red status lamp at the vacuum dega
13. 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 66 1260 Standard Degasser User Manual Identifying Cables Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 me 3 3 Gray Start Low m s NC 4 Blue Shut down Low e O NC 5 Pink Not connected NC 6 Yellow Power on High o 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Standard Degasser User Manual 67 8 Identifying Cables Agilent Module to General Purpose
14. T 500 mA compatible to all supported line voltages Use of unsupplied cables The use of cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 56 The Agilent 1260 Infinity Standard Degasser power supply has wide ranging capability see Table 1 on page 15 It accepts any line voltage in the range mentioned below Consequently there is no voltage selector in the rear of the standard degasser There are two externally accessible fuses that protect the power supply These fuses are identical for all accepted line voltages 1 Switch OFF the power switch at the front of the instrument 2 Remove the power cable from the power connector at the rear of the instrument 1260 Standard Degasser User Manual Maintenance 3 Press down the clip of the fuse holder and pull out of the power socket Fuse holder Figure 12 Fuse Holder and Clip 4 Remove the fuses from the fuse holders Ensure the fuse wires inside the fuses are not broken If a test meter is available check the resistance of each fuse A good fuse shows a low resistance approximately 0 Ohm 6 Ifa fuse is defective wire broken or high resistance insert a new fuse 7 Reinsert the fuse holders and the power cable 8 Switch ON the pow
15. The continuous mode overwrites all other operation modes of the degasser When activated switch SW1 on the electronic board or via the auxiliary cable the vacuum pump is forced into continuous mode and is turned on as long as the degasser is switched ON 1260 Standard Degasser User Manual 9 1 introduction How the Electronics Work 10 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 2 Site Requirements and Specifications Site Requirements 12 Physical Specifications 15 Performance Specifications 16 This chapter provides information on environmental requirements physical and performance specifications te Agilent Technologies 11 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 1 on page 15 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only Module is partially energized when switched off as long as the power cord is plugged in
16. ards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation 72 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation 1260 Standard Degasser User Manual Appendix 9 Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuit
17. asser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the connected pump Priming the standard degasser with a syringe is recommended when standard degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the standard degasser by using the pump at high flow rate 8 5 mL min is recommended when pumping system was turned off for a length of time for example overnight and if volatile solvent mixtures are used or e if solvents have been changed Priming with a Syringe Before using a new degasser or new tubings for the first time WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere 30 1260 Standard Degasser User Manual Installing the Standard Degasser 3 1 Prime all tubings with at least 30 ml of iso propanol no matter whether the channels wi
18. continueing to prime with the pump 1260 Standard Degasser User Manual 31 3 Installing the Standard Degasser Priming with the Pump When the pumping system has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channels between the standard degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Therefore priming of the standard degasser and the pumping system is required before starting an application 1 Open the purge valve of your pump and set flow rate to 5 ml min 2 Flush the standard degasser and all tubes with at least 30 mL of solvent 3 Set flow to required value of your application and close the purge valve 4 Pump for approximately 10 minutes before starting your application 5 Repeat step 1 on page 32 through step 4 on page 32 with other channels where needed 32 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Transporting the Standard Degasser WARNING Solvents leaking out Solvents remaining in the solvent channels may leak out during transport This can possibly cause personal damage gt Drain any remaining solvents from the degassing channels before transporting the degasser Disconnect the solvent tubes from solvent inlets from front panel Disconnect one of the solvent tubes from your pump Connect syringe adapter to solvent tub
19. ctive on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 18 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Delivery Checklist Ensure all parts and materials have been delivered with the degasser The delivery checklist is shown below To aid in parts identification please see Parts and Materials for Maintenance on page 59 Please report missing or damaged parts to your local Agilent Technologies Sales and Service Office Table3 Standard Degasser Delivery Checklist Description Quantity Standard degasser 1 Power cable 1 Remote cable 1 Auxiliary cable As ordered User manual 1 Accessory kit Accessory 1 Kit Contents on page 19 Accessory Kit Contents Accessory Kit p n G1322 68705 p n Description 5062 8534 Syringe Reorder number pack of 10 9301 1337 Syringe adapter 0100 1710 Mounting Tool for Tubing Connections 5062 2463 Corrugated waste tube reorder pack 5 m G1322 67300 Kit of 4 solvent tubes for connection degasser to MCGV Quaternary Pump including labels 1260 Standard Degasser User Manual 19 3 Installing the Standard Degasser Opt
20. e Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter Ifthe application permits add 0 0001 0 001 M sodium azide to the solvent e Place a layer of argon on top of your solvent Avoid exposure of the solvent bottles to direct sunlight Prevent Blocking of Solvent Filters The solvent filters are on the low pressure side of the pumping system A blocked filter therefore does not affect the pressure readings of the pump The pressure readings cannot be used to indetify blocked filters If the solvent cabinet is placed on top of the standard degasser the filter condition can be checked in the following way Remove the tubing at the inlet port of the standard degasser If the filter is in good condition the solvent will freely drip out of the solvent tube due to hydrostatic pressure If the solvent filter is partly blocked no solvent or only very little solvent will drip out of the solvent tube 38 1260 Standard Degasser User Manual Using the Vacuum Degasser 4 Cleaning the Solvent Filters e Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour e Thoroughly flush the filter with bidistilled water remove all nitric acid Replace the filter Never use the system without solvent filter installed 1260 Standard Degasser User Manual 39 4 Using the Vacuum Degasser Operation Modes of
21. e of this solvent channel Push syringe adapter onto syringe ao A Q N Pull syringe plunger to draw solvent out of degasser and tubing Continue to draw solvent into syringe until the solvent channel is completely empty 6 Repeat step 2 on page 33 through step 5 on page 33 for the remaining solvent channels 1260 Standard Degasser User Manual 33 3 Installing the Standard Degasser Transporting the Standard Degasser 34 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 4 Using the Vacuum Degasser When to Use a Vacuum Degasser 36 Solvent Information 37 Prevent Blocking of Solvent Filters 38 Operation Modes of the Vacuum Degasser 40 Normal Operation Mode 1 41 Normal Operation Mode 2 Timing Mode 41 Continuous Mode 42 ErrorMode 42 ee Agilent Technologies 35 4 Using the Vacuum Degasser When to Use a Vacuum Degasser Unspecified Conditions Operating the instrumentation under conditions other than their intended use might result in a potential safety hazard or might damage the instrumentation gt Never operate your instrumentation under conditions other than specified by the vendor 36 Pumps that mix the solvent on the low pressure side need degassing and must be equipped with a degasser or alternative degassing systems for example helium Isocratic pumps and high pressure mixing pumps do not always require degassing However for the following conditions the standard degasser
22. e way connection to send a not ready signal from the degasser to the other modules to shut down the whole system after an error condition of the degasser In an Agilent 1260 Infinity system the individual modules are connected through a CAN cable The Agilent 1260 Infinity Standard Degasser is an exception The standard degasser can be connected via the APG remote connector to the other modules of the stack The AUX output allows to monitor the vacuum pressure in the degasser chamber A G4208A Instant Pilot can be connected to the CAN bus at any of the modules in the system except for the degasser The chromatography data system can be connected to the system through a LAN cable at any of the modules except for the degasser preferably at the detector For more information about connecting the Instant Pilot or a data system refer to the respective user manual Power socket Fuse holder Figure 5 Rear of the Standard Degasser 24 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Installing the Standard Degasser Status indicator Power switch Waste outlet Serial number Figure 6 Front of the Standard Degasser 5 Press in the power switch to turn on the vacuum degasser NOTE The power switch stays pressed in and a green indicator lamp in the power switch is ON when the vacuum degasser is turned ON When the line power switch stands out and the gree
23. ead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment 1260 Standard Degasser User Manual 13 2 Site Requirements and Specifications Bench Space The module dimensions and weight see Table 1 on page 15 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench should carry an Agilent system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 14 1260 Standard Degasser User Ma
24. ed when the lamp is red An error condition exists when the standard degasser detects an internal defect that does not allow the vacuum to be built up in a certain time frame approximately 8 minutes Internal Leak or Electronic Failure If the error LED is on there is either an internal leak in the vacuum system or an electronic failure In case of an internal leak it is possible that solvent may enter the vacuum chamber and solvent may leak into the waste drain gt To prevent any damage of the standard degasser switch off the degasser and remove the solvent bottles from the solvent cabinet to stop any gravity caused flow of solvent into the vacuum chamber 46 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual J e eec 6 e Maintenance J Warnings and Cautions 48 Introduction to Maintenance 50 Simple Repairs Maintenance 50 Using the ESD Strap 50 Cleaning the Instrument 51 Removing and Refitting the Top Cover 52 Assembling the Main Cover 55 Exchanging the Fuses of the Power Inline Filter Exchanging the Status Light Pipe 58 This chapter describes the maintenance of the module ee Agilent Technologies 56 47 6 Maintenance Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures
25. el corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl Oy gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1260 Standard Degasser User Manual 37 4 Using the Vacuum Degasser Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump This is especially true for aqueous solvents or phosphate buffers pH 4 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the pump e Use sterile if possible amber solvent bottles to slow down algae growth
26. el to the back Assure the four metal tabs of the panel slide underneath the Z plane Fix the two holding screws Metal Tabs Screws 6 Replace the top cover Ensure the clips are seated correctly Clips 1260 Standard Degasser User Manual 53 6 Maintenance Introduction to Maintenance 7 Reinstall the front cover 8 Reinstall the degasser in your system stack and connect the cables and capillaries and turn on the degasser 54 1260 Standard Degasser User Manual Maintenance 6 Assembling the Main Cover When e If cover is broken Parts required p n Description 1 5065 9989 Cover kit includes base top left and right CAUTION Wrong assembly In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part gt Take care not to mix up left and right side The cover kit contains all parts but it is not assembled 1 Place the top part on the bench and insert the left and 2 Replace the cover right side into the top part Front Next Steps 3 Replace the degasser in the stack and reconnect the cables and capillaries 4 Turn ON the degasser 1260 Standard Degasser User Manual 55 6 Maintenance Exchanging the Fuses of the Power Inline Filter When Tools required Parts required If the standard degasser appears dead Test meter if available p n Description 1 2110 0458 Fuse 250V
27. er switch 1260 Standard Degasser User Manual 57 Maintenance Exchanging the Status Light Pipe When If part is broken Tools required Screwdriver Pozidriv 1 Parts required p n 1 5041 8384 Description Status light pipe Preparations Remove the front cover and top cover see Removing and Refitting the Top Cover on page 52 2 1 The status light pipe is clipped into the top cover Replace the top cover see Removing and Refitting the Top Cover on page 52 58 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 7 Parts and Materials for Maintenance Cover Parts 60 Power and Status Light Pipes 61 Accessory Kit Contents 62 ee Agilent Technologies 59 7 Parts and Materials for Maintenance Cover Parts Cover Parts Item p n Description 1 5065 9989 Cover kit includes base top left and right 5065 9990 Front cover 5042 8901 Name plate gt wo N 5041 8387 Tube clip Figure 13 Cover Parts 60 1260 Standard Degasser User Manual Parts and Materials for Maintenance Power and Status Ligh Power and Status Light Pipes Item p n 1 5041 8383 2 5041 8382 3 5041 8381 4 5041 8384 Description Power switch coupler Power switch light pipe Power switch button Status light pipe Figure 14 Power and Status Light Pipes 1260 Standard Degasser User Manual 61 7 Parts and Materials for Maintenance Accessory Kit Conte
28. for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the metal top cover of the module No serviceable parts inside gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 48 1260 Standard Degasser User Manual Maintenance 6 CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1260 Standard
29. imizing the Stack Configuration 20 If your standard degasser is part of a system you can ensure optimum performance by limiting the configuration of the system stack to the following configuration This configuration optimizes the system flow path ensuring minimum delay volume from point of solvent mixing to head of column and dead volume from point of injection to detector outlet 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Flow connections in the stack Example setup with 0 17 mm ID green capillaries Solvent bottle pump G1311 60003 bottle head assembly PTFE tubings Pump autosampler G1312 67305 SST green Solvent cabinet Pump purge valve waste 5062 2461 PTFE tubing wide bore reorder pack Degasser Binary pump Instant Pilot G4208A Autosampler Autosampler column compartment G1313 87305 SST green Column compartment column G1316 87300 SST green Column detector DAD G1315 87311 SST coated VWD 5062 8522 PEEK Column compartment Detector waste DAD 0890 1713 PTFE wide bore VWD 5062 8535 PEEK 5062 2463 corrugated waste tubing reorder pack Detector Figure 3 Recommended Stack Configuration Front View 1260 Standard Degasser User Manual 21 3 Installing the Standard Degasser Optimizing the Stack Configuratior Pressure output to recorder CAN Bus cab
30. ing of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Standard
31. le to handheld controller CAN Bus cable for inter module communication 5181 1516 0 5 m 5161 1519 1 0 m Analog signal to recorder Figure 4 22 oo0000 Recommended Stack Configuration Rear View AC power GPIB or LAN to ChemStation AC power 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Installing the Standard Degasser Parts required Description 1 Standard degasser 1 Power cord 1 Interface cable as ordered See Cable Overview on page 64 Preparations Locate bench space Provide power connections Unpack the module CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 1 Place the module on the bench in a horizontal position 2 Ensure the power switch on the front of the module is OFF switch stands out 3 Connect the power cable to the power connector at the rear of the module 1260 Standard Degasser User Manual 23 3 Installing the Standard Degasser 4 Connect the interface cable to the degasser The interface cable remote cable is a on
32. ll be used with organic mobile phase or with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows 2 Replace the current solvent with iso propanol if current solvent is organic or with water if current solvent is an inorganic buffer or contains salt 3 Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump 4 Connect syringe adapter to solvent outlet tube 5 Push syringe adapter onto syringe Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing 7 Replace the priming solvent with the new solvent of your choice Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing 9 Disconnect syringe adapter from solvent tube 10 Connect solvent tube to your pump 11 Repeat step 3 on page 31 to step 10 on page 31 for the other solvent channels When priming the standard degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 min with your selected flow rate before starting any application This will allow the standard degasser to properly degas the solvent in the degasser tubes The pump should never be used for priming empty tubings never let the pump run dry Use the syringe to draw enough solvent for completely filling the tubings to the pump inlet before
33. loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Standard Degasser User Manual Contents Introduction 5 Introduction to the Vacuum Degasser 6 How the Electronics Work 7 Site Requirements and Specifications 11 Site Requirements 12 Physical Specifications 15 Performance Specifications 16 Installing the Standard Degasser 17 Unpacking the Standard Degasser 18 Optimizing the Stack Configuration 20 Installing the Standard Degasser 23 Flow Connections to the Standard Degasser 26 Operational Hints for the Standard Degasser 30 Transporting the Standard Degasser 33 Using the Vacuum Degasser 35 When to Use a Vacuum Degasser 36 Solvent Information 37 Prevent Blocking of Solvent Filters 38 Operation Modes of the Vacuum Degasser 40 Troubleshooting and Diagnostics 43 Overview of the Degasser s Indicators 44 Status Indicators 45 Maintenance 47 Warnings and Cautions 48 Introduction to Maintenance 50 1260 Standard Degasser User Manual Contents Contents 7 Parts and Materials for Maintenance 59 Cover Parts 60 Power and Status Ligh
34. n light is OF the vacuum degasser is turned OFF 1260 Standard Degasser User Manual 25 3 Installing the Standard Degasser Flow Connections to the Standard Degasser Parts required Description Solvent cabinet including solvent bottles filled with solvent and bottle head assemblies Standard degasser Solvent outlet tubes i ae ee Syringe with adapter Preparations Install the degasser WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The amount of substances should be reduced to the minimal volume required for the analysis gt Do not operate the instrument in an explosive atmosphere 26 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Place solvent cabinet with the bottle s on top of the degasser Remove the front cover by pressing the snap fasteners on both sides Figure 7 Removing the Front Cover If the degasser is not used with an Agilent 1260 Infinity pump connect the waste tube from the accessory kit to the waste outlet and place into your waste system Put the bottle head assemblies into solvent bottle
35. ndard Degasser User Manual www agilent com In This Book This manual contains user information about the Agilent 1260 Infinity Standard Degasser The manual describes the following introduction e site requirements and specifications e installing the degasser using the degasser e troubleshooting and diagnostics e maintenance parts and materials for maintenance e identifying cables appendix Agilent Technologies 2006 2008 2010 Printed in Germany 06 10 G1322 90012 fe Agilent Technologies
36. nts 62 Accessory Kit p n G1322 68705 p n 5062 8534 9301 1337 0100 1710 5062 2463 G1322 67300 Description Syringe Reorder number pack of 10 Syringe adapter Mounting Tool for Tubing Connections Corrugated waste tube reorder pack 5m Kit of 4 solvent tubes for connection degasser to MCGV Quaternary Pump including labels 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual Identifying Cables Cable Overview 64 Remote Cables 66 Auxiliary Cable 68 opie Agilent Technologies 63 Identifying Cables Cable Overview 64 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 66 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Agilent module to Agilent 35900 A D converters or HP 1050 1046A 1049A 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Standard Degasser User Manual
37. nual Site Requirements and Specifications 2 Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 7 kg 16 Ibs Dimensions height x width x depth Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating Altitude Non operating altitude Safety standards IEC CSA UL 80 x 345 x 435 mm 3 1 x 13 5 x 17 inches 100 120 220 240 VAC 50 or 60 Hz 5 250 VA 210 W 717 BTU 0 55 C 32 131 F 1 40 70 C 40 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation Category Il Pollution Degree 2 Wide ranging capability Maximum Non condensing For storing the module For indoor use only Research Use Only Not for use in Diagnostic Procedures 1 This temperature range represents the technical specifications for this instrument The mentioned temperatures may not be suitable for all applications and all types of solvents The Agilent 1260 Infinity Standard Degasser has been tested for evaporation of solvents into the atmosphere by an independent institute with approved methods The tests were performed with methanol BIA Nr 7810 and acetonitrile NIOSH Nr 1606 Evaporation of these solvents into the atmosphere when operating the degasser was below the limits of detection 1260 Standard Degas
38. odes are approximate values All values are set in the factory according to the vacuum level Depending on the vacuum sensor batch variations this will result in different voltage readings Regardless of the readings of the various stages no adjustment should be performed on the degasser electronics 1260 Standard Degasser User Manual Using the Vacuum Degasser 4 See service manual troubleshooting and diagnostics for setting up test meter connections to the degasser for reading the pressure sensor output Normal Operation Mode 1 When the degasser is turned ON the vacuum pump runs and is connected to ambient through the solenoid valve The solenoid valve activates about 15 seconds after turning ON the degasser you can hear it click The vacuum pump then begins to pull a vacuum on the vacuum chamber The voltage measurements begin to decrease rapidly The yellow status LED turns off when the vacuum level reaches 190 Torr DC voltage readings around 800 mV The normal operation mode vacuum level 115 Torr is achieved when the DC voltage measures approximately 590 to 600 mV After achieving the normal operation mode vacuum level the solenoid valve turns off The vacuum pump continues to run for a few seconds then it turns OFF When the DC voltage measurement rises back to approximately 600 to 610 mV the turn on process begins again If the vacuum level of normal operation mode one cannot be achieved within 8 minutes the instrument turns in
39. ranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or
40. rements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product s Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Standard Degasser User Manual 75 9 Appendix Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 76 1260 Standard Degasser User Manual Appendix 9 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1260 Standard Degasser User Manual 77 9 Appendix Agilent
41. s containing your mobile phase Connect the solvent tubes from the bottle head assemblies to the inlet connectors A to D typically the left connection of the channel of the degasser Use the mounting tool shown in Figure 8 on page 27 to fix the tube screw Fix the tubes in the clips of the degasser DZ Figure 8 Mounting Tool 1260 Standard Degasser User Manual 27 3 28 Installing the Standard Degasser 6 Connect the outlet tubes to the output ports typically right connection of the channel of the degasser 7 Prime the degasser before first use see Priming the Degasser on page 30 Atmospheric gases can diffuse through the tubing and dissolve in the mobile phase solvents For best chromatographic results keep the length of tubing between the degasser and the pump as short as possible 1260 Standard Degasser User Manual Installing the Standard Degasser 3 Flow Connections to the Standard Degasser Bottle head assembly Solvent cabinet Tube clip not visible Pressure sensor Static mixer Purge valve Pump head channel A Pump head channel B Adapter Active inlet valve B Active inlet valve Waste tubing Outlet capillary to autosampler Waste outlet Figure 9 Flow Connections to the Standard Vacuum Degasser 1260 Standard Degasser User Manual 29 3 Installing the Standard Degasser Operational Hints for the Standard Degasser Priming the Degasser The standard deg
42. ser User Manual 15 2 Site Requirements and Specifications Performance Specifications 16 Table2 Performance Specifications Agilent 1260 Infinity Standard Degasser Type Maximum flow rate Number of channels Internal volume per channel Materials in contact with solvent pH range Analog output AUX Specification 10 mL min per channel 4 Typically 12 mL per channel PTFE PEEK 1 14 For pressure monitoring range 0 3 V 1260 Standard Degasser User Manual 000 o e GS This chapter provides information on unpacking checking on completeness 1260 Standard Degasser User Manual Unpacking the Standard Degasser 18 Damaged Packaging 18 Delivery Checklist 19 Accessory Kit Contents 19 Optimizing the Stack Configuration 20 Installing the Standard Degasser 23 Flow Connections to the Standard Degasser 26 Operational Hints for the Standard Degasser 30 Priming the Degasser 30 Priming with a Syringe 30 Priming with the Pump 32 Transporting the Standard Degasser 33 stack considerations and installation of the module Installing the Standard Degasser ee Agilent Technologies 3 Installing the Standard Degasser Unpacking the Standard Degasser Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defe
43. sser User Manual 7 1 Introduction The amplifier and comparator circuit determines the working range of the vacuum that has to be built up When the vacuum degasser is turned on and the vacuum in the chamber is not within working range above error limit of 190 Torr the amplifier and comparator circuit sends a signal to the vacuum pump driver and the timers of the vacuum pump timer 1 and the solenoid valve timer 2 The vacuum pump is turned on immediately while the solenoid valve closing is delayed by 15 seconds This time delay allows the vacuum pump to start without load before it is connected to the vacuum chamber The status indicator turns yellow when the pump is activated The status lamp is turned off as soon as the vacuum is below the error limit When the vacuum in the chamber reaches its operation mode 1 approximately 115 Torr the amplifier and comparator circuit turns off the solenoid valve and the vacuum pump is turned off by a timer with a delay of 15 seconds As soon as the pressure sensor detects that the limit of the operation mode 1 has been exceeded e g when dissolved gas from the solvent diffused into the vacuum chamber the vacuum pump is started again as described before The pressure signal is available at the auxiliary output It allows to monitor the vacuum system The upper limit of operation mode 1 is 600 mV Values below 600 mV on the pressure output indicate sufficient vacuum in the chamber If the 600
44. sser indicates a problem with the vacuum system or with the electronic control The vacuum degasser generates an error output on the remote lines 1260 Standard Degasser User Manual Troubleshooting and Diagnostics 5 Status Indicators Two status lamps are located on the front of the standard degasser The left lamp indicates the power supply status the right lamp indicates the standard degasser status Status indicator Power supply lamp Figure 10 Location of Status Lamps Power Supply Lamp The power supply lamp is integrated into the main power switch When the lamp is illuminated green the power is ON 1260 Standard Degasser User Manual 45 5 Troubleshooting and Diagnostics Instrument Status Indicator CAUTION The instrument status indicator indicates one of three possible instrument conditions When the lamp is OFF the standard degasser is in ready condition only if the power supply lamp is ON otherwise the instrument is switched OFF or there is a defect in the power supply A ready condition exists when there is sufficient vacuum in the standard degasser A busy condition is indicated when the lamp is yellow A busy condition exists when the vacuum pump of the standard degasser is working to generate or maintain vacuum in the chambers This is the case when the standard degasser is turned ON for the first time or the pressure rises above its limit during normal operation An error condition is indicat
45. t Pipes 61 Accessory Kit Contents 62 8 Identifying Cables 63 Cable Overview 64 9 Appendix 71 General Safety Information 72 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 75 Radio Interference 76 Sound Emission 77 Agilent Technologies on Internet 78 4 1260 Standard Degasser User Manual 1260 Standard Degasser User Manual 1 Introduction Introduction to the Vacuum Degasser 6 How the Electronics Work 7 ee Agilent Technologies 1 introduction Introduction to the Vacuum Degasser The Agilent 1200 Series vacuum degasser model G1322A comprises a 4 channel vacuum container including 4 tubular plastic membranes and a vacuum pump When the vacuum degasser is switched on the control circuit turns on the vacuum pump which generates a partial vacuum in the vacuum container The pressure is meassured by a pressure sensor The vacuum degasser maintains the partial vacuum by turning on and off the vacuum pump depending on the signal from the pressure sensor The LC pump draws the solvents from their bottles through the special tubular plastic membranes of the vacuum container As the solvents pass through the vacuum tubes any dissolved gas in the solvents permeates through the membranes into the vacuum container The solvents will be almost completely degassed when leaving the outlets of the vacuum degasser g Sensor Control i A circuit ar Pump i we L ATA a n
46. t injection precision 36 remote 64 highest retention time reproducibility 36 RS 232 65 humidity 15 CAN cable 24 changing solvents 30 cleaning the instrument 51 1260 Standard Degasser User Manual information Index damaged 19 light pipes 61 missing 19 performance specifications 16 pHrange 16 physical specifications 15 power light pipe 61 power supply lamp 45 power cable 52 power consideration 12 power consumption 15 power cords 13 power supply 56 power switch 23 pressure sensor readings 24 pressure sensor 6 priming withapump 30 32 with a syringe 30 principle of operation 6 radio interference 76 ready condition 46 rear of the vacuum degasser 24 remote cable 66 S safety class 72 safety general information 72 standards 15 symbols 74 screwdriver pozidriv 1 52 58 site requirements power cords 13 snap fastener 27 80 solvent cabinet 52 solvent filters cleaning 39 prevent blocking 38 solvent information 40 sound emission 77 specification performance specifications physical 15 stack configuration 20 status light pipe 58 61 status indicator 45 syringe adapter 31 33 T tool 27 mounting tool 27 tools screwdriver pozidriv 1 52 58 top cover removing and refitting 52 transportation 33 V vacuum pump 6 voltage range 15 voltage selector 56 volume per channel 16 Ww waste electrical and electronic equipment 75 WEEE directive 75 weight 15 when to use adegasser 42 1260 Sta
47. the Vacuum Degasser Table 4 The vacuum degasser allows various operation modes Operation mode 1 and 2 are the normal operation modes of the degasser In operation mode 1 the vacuum degasser works at 115 Torr In operation mode 2 the vacuum degasser works in the range between 115 to 190 Torr The continuous mode can be selected for highest degassing efficiency of the degasser In this mode the vacuum level is below 115 Torr e The error mode is activated in case the degasser cannot achieve a vacuum level of 190 Torr 1 Torr 1 33x10 bar Operation Modes of the Vacuum Degasser Operation Mode 1 Operation Mode 2 Continuous Mode Error Mode Pressure Range Torr DC Voltage Readings mV rough values Operation mode Status Indicator Failure Actions 115 Lower Limit 590 600 Upper Limit 600 610 Pump switched on and off at upper and lower limit during operation OFF below 800 mV YELLOW above 800 mV Switch to Operation Mode 2 115 190 600 800 Pump switched on every 2 minutes for 30 seconds OFF below 800 mV YELLOW above 800 mV Switch to Error Mode Below 115 Below 600 Pump permantly running OFF below 800 mV YELLOW above 800 mV Switch to Error Mode Above 190 Above 800 8 Minutes of continuous pumping before shutdown YELLOW Shut down module switch status indicator to RED 40 The voltage readings given for the various m
48. to normal operation mode 2 Normal Operation Mode 2 Timing Mode Under certain operational conditions large amount of dissolved gas in mobile phase high flow rates the 115 Torr trigger level for operation mode 1 cannot be reached The standard degasser automatically turns into operation mode 2 Normal operation mode 2 is a fixed timing mode Every 2 min the degasser is turned ON for 30 s This assures a pressure level in the range of 115 190 Torr The pressure signal measured with the auxiliary cable is in the range of 600 800 mV If the vacuum level of normal operation mode two cannot be achieved within 8 min the instrument turns into error mode 1260 Standard Degasser User Manual 41 4 42 Using the Vacuum Degasser Continuous Mode The continuous mode is activated either by switching SW1 on the main board to 1 removal of cover is required see Removing and Refitting the Top Cover on page 52 for identifying SW1 see Service Manual Repairs Overview of Internal Parts or by connecting pin 1 white cable and pin 3 green cable of the auxiliary cable to each other When turning ON the vacuum degasser the vacuum pump will run continuously This will establish a vacuum level that is below the trigger level 600 mV 115 Torr of the normal operation mode 1 If the vacuum level of normal operation mode 2 cannot be achieved within 8 minutes the instrument turns into error mode When set to continuous mode the life time of
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Agilent 1260 Infinity Standard Degasser User Manual