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Agilent Technologies VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual

1. PERISTALTIC PUMP Varian Inc Page 22 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 Step 1 Locate the five pin DIN cable in the accessory kit Step 2 Plug one end into the five pin female connector on the Peristaltic Pump rear panel and the other into the female five pin connector on the VK 8020 rear panel labeled PUMP Step 3 Connect a power cord to the rear of the peristaltic pump and plug it into an outlet of the appropriate voltage Step 4 Turn on the pump Warning Ensure the pump is configured for the voltage supplied at the receptacle Step 6 Step 7 Step 8 Plug one end of the four pin cable into the SAMPLING MANIFOLD jack on the VK 8020 rear panel see Figure 1 VK 8020 Cable Connections on page 21 and the other end into the corresponding jack on the dissolution apparatus rear panel Plug one end of the four pin cable into the INJECTOR CONTROL jack on the VK 8020 rear panel see Figure 1 VK 8020 Cable Connections on page 21 and the other end into the INPUT jack on the VK 8020 HPLC Autoinjector Station rear panel Plug one end of the four pin cable with the two free wires on one end into the START OUTPUT jack on the VK 8020 rear panel Connect the black wire to the positive closure on the HPLC and the other wire to the negative closure on the HPLC Plug one end of the
2. NOTICE This document contains references to Varian Please note that Varian Inc is now part of Agilent Technologies For more information go to www agilent com chem 4 Agilent Technologies VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual P N 70 9028 November 2010 Revision E Limitation of Liability The information in this document is subject to change without notice Varian Inc makes no warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Varian Inc shall not be liable for errors contained herein or for incidental consequential damages in connection with the furnishing performance or use of this material All rights are reserved Reproduction adaptation or translation without prior written permission is prohibited except as allowed under copyright laws First Edition November 2010 VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual Part Number 70 9028 Revision E Printed in the United States of America The following terms are trademarks of Varian Inc Benchsaver e Practical Solutions BIO DIS III QA and QAII Enhancer Cell e TruCenter Full Flow Filters VanKel e Peak Vessel VK All other trademarks are the property of their respective owners Varian Inc 13000 Weston Parkway Cary North Carolina 2
3. Remove the line cord from the VK 8020 Insert a tiny screw driver into the release slot on the left side of the fuse compartment closest to the power switch A slight application of pressure to the left will release the compartment 8 gt 5 gt N aq 3 A O 4 Pull the fuse compartment out of the line cord socket The fuse is located in the removable holder The fuse is a 2 0 amp metric 5 x 20 mm standard fuse Replace the fuse in the holder and insert it back into the fuse compartment Insert the fuse compartment into the line cord socket The compartment is keyed and can only be inserted one way Push the fuse compartment into the line cord socket until both sides snap into position Replace the line cord Varian Inc Revision E 11 10 P N 70 9028 VK 8020 Sample Collector HPLC Autoinjector Page 67 Station Operator s Manual Maintenance and Troubleshooting Volumetric Calibration It is critical that sample volumes are accurately and precisely taken Calibration of your system ensures this by recording in memory the time required to deliver a nominal 10 mL of medium into the graduated calibration tubes Refer to Automatic Calibration below or Manual Calibration on page 68 for specific operating procedures necessary to calibrate your VK 8020 Note Op Ensure filters are in place during calibration Automatic Calibration To perform automatic calibration com
4. with respect to this product Specifically disclaimed are any implied warranties of merchantability and fitness for a particular use In no event will Varian Inc be liable for any indirect incidental or consequential damages arising from the use of this product This warranty gives you specific legal rights which may vary from state to state or province to province so you may have other rights and some of these exclusions may not apply to you Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 75 P N 70 9028 Operator s Manual Service and Warranty Exclusive Remedies The remedies provided herein are the customer s sole and exclusive remedies In no event shall Varian Inc or its representatives be liable for any direct indirect special incidental or consequential damages whether based on contract tort or any other legal theory Some states or provinces do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you Varian Inc Page 76 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Service and Warranty Operator s Manual P N 70 9028 This page was intentionally left blank except for this message Varian Inc Index a autocalibration block installing 27 automatic calibration 67 c calibration completion 36 cartridge ribbon 60 cartridges peris
5. 2 Place sample tubes or autosampler vials in the sample tray Note that although the VK 8020 can fill eight rows of tubes or vials with each row corresponding to a programmed sample point it is not necessary to fill all rows For example if three sample points are programmed only rows 1 through 3 need to be filled Note The first sample always moves into row 1 Varian Inc Page 27 Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Setting Up the VK 8020 P N 70 9028 Operator s Manual Installing the Autocalibration Block and the Media Rinse Reservoir The autocalibration block and media rinse reservoir fit in the rear of the unit between the metal brackets The dispensing arm must be moved forward to put them in place Step 1 Press MENU gt 7 gt 4 gt 9 to move the dispensing arm forward to row 9 The autocalibration block is placed in the rear most position and the media rinse reservoir is placed in front of it Both items fit between the metal brackets Note 4 The replacement media portion of the reservoir has two a sm barbed fittings one on each end of the tank The rinse tray portion of the reservoir has a single barbed fitting Orient the reservoir with the rinse tray portion towards the front Step 2 Press H to return the dispensing arm to its home position Step 3 Press ESC three times to return to the Ready screen Step 4 Connect a nin
6. CAL and CLEAN SYSTEM keys on the VK 8020 Use this option to control the sipper needle See Main Menu Option 6 Injection Control on page 42 7 Manual Operation Use this option to manually control the VK 8020 See Main Menu Option 7 Manual Operation on page 45 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 35 P N 70 9028 Operator s Manual Operation Main Menu Option 2 Set Alarms Select option 2 Set Alarms from the Main Menu The Activate Deactivate Alarms screen displays ACTIVATE DEACTIVATE ALARMS 1 ELAPSED TIMER 2 ROW SAMP COMPLETION 3 PRG COMPLETION 4 CALIB COMPLETION TIME 06 05 03 00 00 00 ELAPSE 000 00 00 Following is a description of the Activate Deactivate Alarms screen options Option Function 1 Elapsed Timer The elapsed timer alarm is a simple timer you can use to time any interval from 1 minute to 99 hours The system beeps when the time has expired It continues to beep until any key is pressed Once turned off the timer does not operate again until you reset the timer To activate the timer alarm complete the following steps Step 1 Select option 1 Elapsed Timer Step 2 Enter a time in hh mm format and press ENTER Step 3 Press any key to silence the alarm after it sounds 2 Row Samp The alarm beeps to indicate the completion of each sample Completion point This alarm is useful when the sam
7. Options screen displays HPLC INJECTION OPTIONS 1 DISABLE ENABLE INJECTION 2 INJECT INTERVAL 3 INJECT STANDARDS 4 MULTI INJECTION 5 ON OFF WAIT FOR SIGNAL 6 SET PARAMETERS Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 37 P N 70 9028 Operator s Manual Operation Following is a description of the HPLC Injection Options screen options Option Function 1 Disable Enable Select option 1 to toggle between ENABLE INJECTION Injection and DISABLE INJECTION The option displayed is the opposite of the current setting For example if DISABLE INJECTION displays the injector functions are enabled Disabling the HPLC injector functions allows you to use the system only as a collector 2 Inject Interval Select option 2 Inject Interval to specify the time between injections Enter the desired time from 1 to 99 minutes and press ENTER If the unit is in the Wait for Signal mode see 5 On Off Wait for Signal on page 38 the time specified is the minimum time interval For example if the external signal is received after the time interval the injection cycle starts immediately However if the external signal is received before the end of the interval the system waits until the end of the interval to begin the next cycle 3 Inject Standards Select option 3 Inject Standards to specify the regularity of standard injections during a sequence choose
8. Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Page 61 P N 70 9028 Station Operator s Manual Maintenance and Troubleshooting Step 3 Push down on the right side of the ribbon cartridge marked PUSH and remove the old cartridge Step 4 Install the new cartridge If there is already paper in the printer hold the cartridge between your thumb and index finger slide it over the paper and into the printer compartment Ensure the paper is between the ribbon cartridge and the ink ribbon Ensure the ink cartridge is inserted firmly to prevent weak or irregular printing The cartridge must be properly seated and aligned for the best printing Step 5 Turn the cartridge knob marked by an arrow clockwise to stretch the ribbon taut Step 6 Replace the cover Step 7 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position The Ready LED illuminates Step 8 Replace the paper if necessary If you get ribbon ink on the printer s plastic cover remove it immediately Once dried it is difficult to remove Replacing the Paper Roll Step 1 Step 2 Step 3 Step 4 Toggle the printer off line by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Grasp the paper roll cover firmly by the grooves on the side and the front edge Pull outward to remove the cover Press the printer toggle swi
9. and needles clean The timing of the valves opening and closing with the pump rotations creates cavitation in the valves Cleaning the system dislodges any particulate matter that may affect the operation of the system Note a Prior to beginning the clean system function verify an aq La appropriate manual prime time and purge time have been entered See 4 Manual Prime Time on page 34 and 5 Manual Purge Time on page 34 To clean the system complete the following steps Step 1 Place the cleaning tray on the vessel plate of the dissolution apparatus Step 2 Fill the cleaning tray with an appropriate cleaning solution usually water Step 3 Lower the sampling cannulas Step 4 On the VK 8020 press CLEAN SYSTEM The Cleaning Systems Selections screen displays To start the cleaning process immediately see Start Immediately on page 57 To program the auto clean function see Programmed Clean on page 57 CLEANING SYSTEMS SELECTIONS 1 START CLEAN 2 ENABLE AUTO CLEAN TIME 06 05 03 08 28 46 ELAPSE 000 00 00 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Page 57 P N 70 9028 Station Operator s Manual Maintenance and Troubleshooting Start Immediately From the Cleaning Systems Selections screen select option 1 Start Clean The VK 8020 immediately starts to clean the system by priming the lines Once the system completes the prim
10. four pin cable into the START INPUT jack of the VK 8020 and the other end into the START OUTPUT jack of the dissolution apparatus Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 23 P N 70 9028 Operator s Manual Setting Up the VK 8020 Step 9 Connect power cords to the VK 8020 and the HPLC autoinjector station and plug them into outlets of the appropriate voltage Warning Before plugging the VK 8020 into any power outlet ensure the instrument is configured for the voltage provided Check the serial number tag on the rear panel to confirm the voltage requirement Connecting the VK 8020 Autosampler to HPLC Refer to this diagram to connect the sample collector to the autoinjector station FIGURE 2 Connecting the Autosampler to the HPLC TO HPLC TO HPLC COLUMN PUMP NEEDLE j VALVE TATUS av 2 3 p 6 7 6 o Varian Inc Page 24 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 Step 1 Connect the sample loop to the Rheodyne valve at positions 1 and 4 Step 2 Connect the three foot tubing from the sipper needle to the Rheodyne valve at position 6 Step 3 Connect the tubing from the HPLC pump to position 2 of the Rneodyne valve Step 4 Connect the four foot 0 042 inch ID tubing to the Rheodyne v
11. sample rows to be analyzed and specify placement of standard solutions See Injection Option 3 Inject Standards on page 38 4 Multi Injection Select option 4 Multi Injection to program up to nine replicate injections from each vial The specified number of injections that will be made from each vial including standards displays on the screen Varian Inc Page 38 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Option Function 5 On Off Wait for Signal Select option 5 to toggle between ON WAIT FOR SIGNAL and OFF WAIT FOR SIGNAL To make injections only when both an external signal is received by the system and a minimum time interval has passed ensure option 5 indicates ON WAIT FOR SIGNAL In the Wait for Signal mode the time interval specified under Main Menu option 3 will be the minimum time interval For example if the external signal is received after the time interval the injection cycle starts immediately However if the external signal is received before the end of the interval the system waits until the end of the interval to begin the next cycle 6 Set Parameters Select option 6 to set the fill volume flush volume fill speed dispense speed inject delay and load delay as well as to enable or disable the filter changer Values for options 1 5 on the HPLC Injection Options screen are individually set for eac
12. standards to be injected after each row Standards are positioned from left to right corresponding to the sample rows to which they relate For example the standard vial in row S1 position 3 will be injected at the beginning of row 3 The standard vial in row S2 position 5 will be injected after the samples in row 5 If rows are to be bracketed by a standard a vial containing the same standard must be placed in both rows S1 and S2 Multilevel standards may be used by placing a different standard concentration in each position If standards are not being injected then rows S1 and S2 should remain empty Injection Option 6 Set Parameters From the HPLC Injection Options screen select option 6 Set Parameters The HPLC Parameters screen displays HPLC PARAMETERS 4 DISPENSE SPEED 1 FILL VOLUME 5 INJECT DELAY 2 FLUSH VOLUME 6 LOAD DELAY 3 FILL SPEED 7 FILTER CHANGE Varian Inc Page 40 Operation VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operator s Manual P N 70 9028 Following is a description of the HPLC Parameters screen options Note IE The values for these parameters pertain to all programs and manual actions Option Function 1 Fill Volume Select option 1 Fill Volume to specify the volume to be withdrawn to fill the loop Since larger or smaller syringes may be used in the system this feature allows you to change syringes without changing th
13. start the next cycle The flexible design of the VK 8020 permits the use of either module as a stand alone component at any time Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 17 P N 70 9028 Operator s Manual Introduction Introduction to the HPLC Autoinjector The sample collector is available in a modified format to interface with the HPLC autoinjector station allowing direct on column injection of sample aliquots immediately after they are collected from the dissolution vessels In this model there are two primary differences e Asipper needle is included in the sampling valve head This needle moves from side to side and up and down to pull aliquots from the collection tubes or vials for transfer Using the autoinjector station samples are loaded into the Rneodyne valve housed in the VK 8020 e Two additional options display on the sample collector Main Menu 1 Option 3 Injct Options allows you to set values for all relevant injection parameters 2 Option 6 Injection Control allows you to manually control the Rneodyne injector valve and the syringe pump When coupled with the autoinjector station the sample collector can be used to collect samples and inject samples either separately or in tandem Warning The VK 8020 contains electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause
14. symbol on the product or on its packaging indicates that this product must not be disposed of as unsorted municipal waste The separate collection and recycling of your waste equipment at the time of disposal will help to conserve natural resources and ensure that it is recycled in a manner that protects human health and the environment For more information on collection reuse and recycling systems please contact your local regional waste administration your local distributor or Varian Inc Varian Inc chapter2 Introduction The VK 8020 provides an easy way to collect samples from a dissolution apparatus and inject them onto an HPLC column Varian Inc Page 16 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Introduction Operator s Manual P N 70 9028 The VK 8020 is compatible with most HPLC systems and consists of two main modules e A sample collector with Peristaltic Pump which incorporates all the features of the VK 8000 Sample Collector e An autoinjector station which handles the injection of sample aliquots onto the HPLC column Sample volumes from 1 to 14 mL can be taken from the vessels with up to eight sampling points per run In addition sampling programs can be set up to run for up to 999 hours The automated sampling cannulas lower and raise at each sample point automatically Microprocessor controlled valves ensure accurate volume transfer of the sample from vessel to coll
15. the outside notches Refit the cartridge to the pump by hooking the clip on the bottom left of the cartridge into the groove on the left side of the pump Push down on the right side of the cartridge ensuring that the tubing remains under the cartridge and pull up the lever to lock the cartridge in place The excess pump tubing can be trimmed to within one inch of the colored tubing clips E v gt ays I gt The cover does not fit unless the cartridges are positioned Note with the levers on the right side Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 21 P N 70 9028 Operator s Manual Setting Up the VK 8020 Step 6 Repeat steps 3 5 for each remaining cartridge Step 7 Refit the cover and replace the two thumb screws If the cover is not replaced correctly the pump will not operate Cable Connections Warning The electrical connection at the back of the collector and autoinjector station is the primary disconnect for the instruments The instruments should be positioned to allow accessibility to the power cords for easy disconnection Refer to this diagram to correctly make cable connections for the VK 8020 HPLC autoinjector station Peristaltic Pump and dissolution apparatus FIGURE 1 VK 8020 Cable Connections VK 7000 Spices HPLC Injector Station TOHPLG n r VK 8020 mM OOO ces 000s D fe 1
16. 7513 2250 800 229 1108 919 677 1108 Fax 919 677 1138 www varianinc com VARIAN Copyright c 1999 2010 by Varian Inc Table of Contents Chapter 1 Safety Practices and Hazards 9 Electrical Hazards 10 Other 10 General 13 WEEE Directive 14 Chapter 2 Introduction 15 Introduction to the HPLC Autoinjector 17 Conventions Used in this Manual 18 Varian Inc Table of Contents Chapter 3 Setting Up the VK 8020 19 Unpacking Your VK 8020 19 Setting Up the Peristaltic Pump 20 Cable Connections 21 Connecting the VK 8020 Autosampler to HPLC 23 VK 8020 Initial Power Up 24 Installing the Sample Tray 26 Installing the Autocalibration Block and the Media Rinse Reservoir 27 Sipper Needle Installation 28 Hidden Key Functions 29 Chapter 4 Operating the VK 8020 31 VK 8020 Keypad Options 31 Main Menu 33 Main Menu Option 2 Set Alarms 35 Main Menu Option 3 Injection Options 36 Main Menu Option 6 Injection Control 42 Selecting the Vial Position 43 Main Menu Option 7 Manual Operation 45 Report Center Printer Operation and Communications 46 Programming the VK 8020 48 Program Variables Option 2 Sample Time Points 50 Start Program 51 Manual Sample 52 Varian Inc Table of Contents Chapter 5 Maintenance and Troubleshooting 55 General Maintenance 55 Clean System Function 56 Start Immediately 57 Programmed Clean 57 Cleaning Tips for the VK 8020 60 Report Center Impact Printer 60 Installing
17. Center Printer Operation and Communications on page 46 CAL Use this option to calibrate the VK 8020 to ensure that sample volumes are accurately and precisely taken See Volumetric Calibration on page 67 PROG Use this option to set program parameters See Programming the VK 8020 on page 48 START PROG Use this option to start a program or modify a program already operating See Start Program on page 51 MANUAL SAMPLE Use this option to collect a sample from the dissolution vessels on command without a program See Manual Sample on page 52 OPEN VALVES Press OPEN VALVES to open all valves simultaneously This function keeps the valves open as long as the key on the keypad is pressed To change the valve configuration press MENU gt 0 gt 0 and select the appropriate configuration AY PROBES Press PROBES UP DOWN to raise or lower the UP DOWN sampling probes on the VK 8020 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 33 P N 70 9028 Operator s Manual Operation Option Function PUMP OFF PUMP OFF PUMP FWD and PUMP REV keys are used to control PUMP FWD the pump attached to the system Pressing either PUMP FWD or PUMP REV PUMP REV causes the pump to operate in the indicated direction PUMP OFF stops the pump no matter in which direction it was operating It is best to stop the pump before changing the dire
18. IALIZING PLEASE WAIT TIME 06 05 03 21 56 40 ELAPSE 000 00 00 Note This is the only time the firmware revision level displays Record the number here and refer to it if you need to call the Dissolution Systems Service Department Sampling station serial number Firmware version While the unit is powering up the dispensing arm returns to its home position at the rear of the unit and the firmware is initialized If the dispensing arm is already at the home position when the unit is powered up the initial status screen displays for three seconds only When the initial status screen disappears the Ready screen displays see Figure 4 Ready Screen below Turn on the HPLC autoinjector by pressing the switch located on the rear panel AC power connector The autoinjector initializes FIGURE 4 Ready Screen READY PRG 01 R M ENABLED VOLUME 5 mL TIME 11 10 00 21 56 40 ELAPSE 000 00 00 Varian Inc Page 26 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 Installing the Sample Tray Step 1 Place the supplied sample tray on top of the unit The sample tray has four grommeted corner holes which fit over the four locating pins Caution The sample tray must be securely mounted on the locating pins Otherwise damage can occur to the sampling needles and drive mechanism Step
19. W 0 VOLUME 5ml TIME 06 05 03 09 37 14 ELAPSE 000 00 00 This menu accepts sampling times and saves them for execution when the program is run Timepoints are requested in order of execution starting with collection row number zero The sample time is entered as hours and minutes Once you enter a time the row number increments and another sample timepoint is requested Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 51 P N 70 9028 Operator s Manual Operation The following conventions are used with this menu e Review previously entered sample timepoints by pressing ENTER to scroll through the entries e After all sample timepoints have been entered enter 000 00 for the last sample timepoint and press ENTER e To add additional sample timepoints press ENTER until a new row time displays Enter the additional sample timepoint e To correct an entry press BACKSPACE to go to the previous character position and re enter the correct value e Ifa shorter sample timepoint than the previous one is entered for a given row the row number will not increment Times must increase from one row to the next e To shorten a saved program enter 000 00 for the first sample timepoint you wish to discard All successive timepoints are eliminated e Press ESC to save all new values and return to the Program Variables screen Start Program Caution Do not leave the cover on the dispens
20. ach program number and is independent of the manual prime time or the prime time in other programs Varian Inc Page 50 Operation VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operator s Manual P N 70 9028 Option Function 6 Purge Time Select option 6 Purge Time The Purge Time screen displays Enter a value for the purge time from 1 to 99 seconds and press ENTER The purge time is the interval the Peristaltic Pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn Purging also clears the sampling filters of particulate matter that may restrict the successive samples As with priming the longer the tubing lengths the longer the purge time required Note purge time is unique to each program number and is independent of the manual purge time or the purge time in other programs 7 R M Options Select option 7 R M Options The Replacement Media Option screen displays two options enable and disable Ensure the asterisk displays next to option 1 Disable if the media replacement option is not installed Note the current setting is indicated with an asterisk Program Variables Option 2 Sample Time Points From the Program Variables screen select option 2 Sample Time Points The Sample Time Point screen displays SAMPLE TIME POINT 000 02 ENTER hh mm UP TO 999 59 HOURS PROG 1 RO
21. alve at position 5 and to the left side of the two way syringe valve Step 5 Remove the small screw located on the piston on the autoinjector station and secure the 500 ul syringe to the two way syringe valve Align the hole in the syringe plunger to the hole in the piston Replace the screw Step 6 Connect the 18 inch tubing to the right side of the two way syringe valve and place the free end into a 500 mL bottle containing ultrapure water Step 7 Connect the tubing from the HPLC column to position 3 of the Rneodyne valve VK 8020 Initial Power Up A Warning The VK 8020 has a dual voltage 115 V 230 V option on the power entry module Ensure the voltage indicated on the fuse drawer matches the power outlet The electrical connection at the back of the apparatus is the primary disconnect for the instrument Turn on the VK 8020 by pressing the switch located on the rear panel AC power connector The system monitor illuminates The system monitor displays the initial status screen see Figure 3 Initial Status Screen on page 25 and then changes to the Ready screen see page 25 From the Ready screen you can access the Main Menu or any of the manual functions Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 25 P N 70 9028 Operator s Manual Setting Up the VK 8020 FIGURE 3 Initial Status Screen VK 8020 FRACTION COLLECTOR PROGRAM REVISION X XX INIT
22. ample lines After purging the autoinjector station begins the injection cycle The syringe is primed and the samples are injected according to the parameters set under injection options see Main Menu Option 3 Injection Options on page 36 This option uses the values entered under options 2 3 4 5 and 6 for manual sampling The display screen keeps the operator informed as each step of the manual collection takes place e Option 2 Start without HPLC begins the sequence by priming the sample lines Next the appropriate volume of sample is pulled followed by purging of the sample lines e Option 3 Modify HPLC Setting allows the operator to change any of the HPLC Injection options such as inject standards number of injections and time between injections See Main Menu Option 3 Injection Options on page 36 Note select this option last 2 Set Row Select option 2 Set Row Enter a row number between 1 and 8 and press ENTER The Manual Sampling screen displays 3 Set Volume Select option 3 Set Volume Enter a volume between 0 and 14 and press ENTER The volume can contain a decimal point so that fractional milliliters can be collected Do not request more volume than the tubes or vials can contain 4 Prime Time Select option 4 Prime Time Enter a duration between 1 and 99 seconds that the pump will run in the forward direction with the valves in the bypass position and press ENTER The time sh
23. and right edges can be trimmed or shaved with a utility knife allowing the cover to slide easier Step 14 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position The Ready LED illuminates Varian Inc Revision E 11 10 P N 70 9028 VK 8020 Sample Collector HPLC Autoinjector Page 63 Station Operator s Manual Maintenance and Troubleshooting Toggling Your Printer Online Complete these steps to toggle your printer online Step 1 Step 2 Toggle the printer online by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Release the switch and it returns to the center position The Ready LED illuminates and a READY message prints if the PRINT READY command has not been turned off See Printer Configuration on page 64 for instructions on turning on and off the PRINT READY command When you first turn on the instrument it prints a READY message to assure you that the built in microprocessor is operating properly When you turn off the printer wait at least three seconds before turning it on again Printer Self Test You can test the print head and ribbon only after inserting paper Do not attempt to print without paper Follow these steps to perform a printer self test Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Turn off the VK 8020 Press and hold the printer toggle swi
24. anually Use injection control to check proper operation for duplicate programmed operations such as filling the sample loop manually From the Main Menu select option 6 Injection Control MOVING TO RINSING POSITION displays The Sipper Needle Position screen displays SIPPER NEEDLE POSITION 1 8 ROW 1TO8 R RINSE POSITION S ROW S1 N ROW S2 TIME 06 05 03 14 45 41 ELAPSE 000 00 0 Following is a description of the Sipper Needle Position screen options Option Function 1 8 ROW 1TO8 Pressing a number 1 8 indicates which row to move the sipper needle for a manual injection See Selecting the Vial Position on page 43 R RINSE POSITION Press R to move the dispensing arm to the rinse chamber of the media rinse reservoir S ROW S1 Press S to move the dispensing arm to row 0 or the first row of standards N ROW S2 Press N to move the dispensing arm to row 9 or the second row of standards Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 43 P N 70 9028 Operator s Manual Operation Selecting the Vial Position From the Sipper Needle Position screen enter a row number to move the sipper needle for a manual injection The HPLC Module Control screen displays HPLC MODULE CONTROL 1 6 VIAL 1 TO 6 S SYRINGE CONTROL V INJ VALVE I INJECT P PRIMING F FILTER CHANGER L LOWER NEEDLE Following i
25. ction of rotation CLEAN SYTEM Use this option to keep the valves needles and fluid lines clean See Clean System Function on page 56 LOCK KBRD Press LOCK KBRD to lock the keypad Press LOCK KBRD again to unlock the keypad Main Menu Press MENU to access the Main Menu The Main Menu displays Set all parameters before starting a program because the Main Menu cannot be accessed if a program is running Press ESC from the Main Menu to return to the Ready screen The following sections describe in detail the use of each of the seven options available from the Main Menu MAIN MENU 4 MANUAL PRIME TIME 1 SET CLOCK 5 MANUAL PURGE TIME 2 SET ALARMS 6 INJECTION CONTROL 3 INJCT OPTIONS M 7 MANUAL OPERATION Following is a description of the Main Menu screen options Option Function 1 Set Clock Use this option to set the clock Select option 1 Set Clock Enter the correct date and press ENTER Enter the correct time and press ENTER The Main Menu displays Varian Inc Page 34 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Option Function 2 Set Alarms Use this option to set the elapsed timer as well as the row sample completion alarm program completion alarm and calibration completion alarm See Main Menu Option 2 Set Alarms on page 35 3 Injct Options M Use this option
26. death serious injury or painful electric shock Caution Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Varian trained Varian qualified or Varian authorized service engineers Varian Inc Page 18 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Introduction Operator s Manual P N 70 9028 Conventions Used in this Manual e Items you are asked to press are in bold For example press H on the keypad e Key sequences you are asked to press appear like this MENU gt 7 Note Remember to return the warranty card supplied with this manual Completing and returning the card ensures your right to L__ gt protection under the terms and conditions of your warranty It also enables us to better assist you in the event of any problems Additionally it guarantees you will be informed of any issues that arise concerning your equipment such as upgrades retrofits or regulatory changes Varian Inc Chapter 3 Setting Up the VK 8020 Unpacking Your VK 8020 Complete the following steps to unpack your VK 8020 Step 1 Open each carton and check the contents for damage which may have occurred during shipping Shipping damage rarely occurs but if it does contact both the carrier who delivered the instruments and the Dissolution Systems Service Department Though claims for damage should be
27. e firmware The standard syringe supplied with the VK 8020 has a volume of 500 ul Therefore entering 50 causes the syringe to withdraw approximately 250 ul to fill the loop for each injection Note even though the loop itself may have a volume of only 20 ul several times that volume must be withdrawn due to the length of the tubing between the sipper needle and the loop as well as the need to overfill the loop to flush it thoroughly 2 Flush Volume Select option 2 Flush Volume to specify the flush volume and the number of flush cycles After each injection the connecting tubing and the inside of the sipper needle need to be flushed to minimize carryover between injections The two way valve at the top of the syringe switches to the right to bring the flush solvent bottle online and the syringe withdraws to fill with flush solvent Then the valve switches back to the left toward the injector valve and the syringe pushes the flush solvent back through the valve and sipper needle into the waste section of the media rinse reservoir As with filling flush volumes are entered as a percentage of the syringe volume At the appropriate screen specify the number of extra consecutive flush cycles Each extra flush cycle is 90 of a full syringe volume Multiple flush cycles ensure all sample is removed from the lines before filling for the next injection Some applications may require a single flushing cycle while others may
28. e pin cable from the right side of the autocalibration block to the port labeled CALIBRATION in the rear of the VK 8020 Step 5 Connect the six foot length of clear tygon tubing to the barbed elbow fitting located on the rinse tank portion of the reservoir Place the other end in a sink drain or suitable waste container Varian Inc Page 28 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 Sipper Needle Installation Remove the needle from the storage box and check it for damage Ensure the needle support carriage is in the up position by completing the following steps Step 1 Press MENU Step 2 Select option 6 Injection Control The Sipper Needle Position screen displays SIPPER NEEDLE POSITION 1 8 ROW 1TO8 R RINSE POSITION S ROW S1 N ROW S2 TIME 06 05 03 14 45 41 ELAPSE 000 00 0 Step 3 Enter 1 for row 1 The HPLC Module Control screen displays HPLC MODULE CONTROL 1 6 VIAL 1 TO 6 S SYRINGE CONTROL V INJ VALVE I INJECT P PRIMING F FILTER CHANGER R RAISE NEEDLE Step 4 If RAISE NEEDLE displays on the screen press R Raise Needle When the needle is in the up position the system monitor displays LOWER NEEDLE Step 5 Place the metal ferrule and nut over the needle and slide the needle into the needle support carriage Step 6 Loosely secure the nut around the sipper
29. ector and the sample collector s autocalibration feature makes periodic calibration of the valves quick and easy The unique needle manifold allows samples to be delivered into either open tubes or pre capped HPLC vials eliminating any concerns about evaporation or contamination The autoinjector station incorporates a quality Rneodyne 7710 injector valve anda syringe pump which pulls samples into the injection loop prior to each injection and flushes the lines afterward Injection intervals are allowed from 1 to 99 minutes and multiple injections can be made from each tube or vial You can independently program the number of standard injections to meet the USP system suitability criteria Standard injections can be programmed before after or bracketing the sample injections Both VK 8020 modules communicate intelligently with each other and with the HPLC system allowing the autoinjector station to make as many injections as possible between sample timepoints During a sample timepoint the autoinjector station goes into standby mode returning to its task only when the sample timepoint is complete Consequently efficiency is maximized and samples subject to evaporation and degradation do not sit for long periods of time before injection The autoinjector station sends a TTL signal to the HPLC at each injection signaling the beginning of a run It can also be programmed to wait for a signal before injecting ensuring the HPLC system is ready to
30. es the pump reverses and purges the lines and the dispensing arm returns to its home position The calibration completion alarm if enabled sounds when the calibration sequence is finished Note Because of intrinsic variations in tubing diameters internal valve a M a U passages and pump tubing tensions some valves must stay open longer than others to deliver their nominal 10 mL calibration volumes This is normal operation Step 6 Once the calibration procedure is completed remove and clean the autocalibration block Manual Calibration If you do not have an autocalibration block or prefer to perform the calibration yourself complete the following steps Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Remove the sample tray Press MENU Select option 7 Manual Operation Select option 4 Goto Rows Enter 4 to go to row 4 Remove the media rinse reservoir Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Page 69 P N 70 9028 Station Operator s Manual Maintenance and Troubleshooting Step 7 Remove the top portion of the autocalibration block and place it on its side to prevent damage Step 8 Place clean empty 13 mL calibration tubes in the bottom portion of the autocalibration block Step 9 Press H to return to the home position Step 10 Press ESC three times to return to the Ready screen Step 11 Fill the cleaning tray with tap water a
31. es screen displays PROGRAM VARIABLES 4 HEADERS 1 HPLC INJECT OPTIONS 5 PRIME TIME 2 SAMPLE TIME POINTS 6 PURGE TIME 3 SAMPLE VOLUME 7 R M OPTIONS Note The VK 8020 can store up to 30 programs in non volatile memory gt _ valid program numbers are 1 to 30 If zero or a number larger than 30 is entered the system continues to prompt you until a valid number is entered A leading zero is optional for programs 1 through 9 Following is a description of the Program Variables screen options Option Function 1 HPLC Inject Options Select option 1 HPLC Inject Options The HPLC Injection Options screen displays The HPLC Injection Options screen can also be accessed via option 3 Injct Options on the Main Menu for non programmed injections For a full description of these options see Main Menu Option 3 Injection Options on page 36 2 Sample Time Points Select option 2 Sample Time Points to enter the sample timepoints See Program Variables Option 2 Sample Time Points on page 50 3 Sample Volume Select option 3 Sample Volume Enter a volume up to 14 0 mL and press ENTER The volume can be up to three digits with a decimal point for example 13 5 Verify the collection tubes can hold the requested volume Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 49 P N 70 9028 Operator s Manual Operation Option F
32. eys function exactly like their counterparts on a personal computer or typewriter keyboard See Figure 5 VK 8020 Keypad below A discussion about each of the main function keys follows FIGURE 5 VK 8020 Keypad VK 8000 Varian Inc Page 32 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 The front panel options include the following Option Function ESC Press ESC to stop execution of a running collection injection program or calibration routine Press ESC to move back one level to the previous menu or selection when moving through the multi level menu Be very careful when using ESC while a program is running If a program or calibration is running and ESC is pressed a prompt appears on the first line of the display asking if you want to stop the running program The options are Y or N Press Y to stop the running program Press N to continue the running program MENU Use this option to set the clock alarms injection options manual prime time manual purge time as well as to control the injection and manually control the sipper needle and the VK 8020 See Main Menu on page 33 PRINT Press PRINT to record the batch information print the sample times enable or disable the Report Center Printer select a local or remote printer set the communication port identification number and set the baud rate See Report
33. filed with the carrier we can help you file a claim Step 2 Carefully remove the VK 8020 HPLC autoinjector station accessory kit and pump from their shipping cartons Step 3 Remove as much cushioning material and tape as possible Varian Inc Page 20 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 Step 4 Place the equipment on a clear dry level section of the bench top close to the dissolution apparatus The preferred placement of the VK 8020 and HPLC autoinjector station is on the right side of the dissolution apparatus with the Peristaltic Pump between the dissolution apparatus and the VK 8020 A Warning The electrical connection at the back of the collector and autoinjector station is the primary disconnect for the instruments The instruments should be positioned to allow accessibility to the power cords for easy disconnection Setting Up the Peristaltic Pump Step 1 Step 2 Step 3 Step 4 Step 5 Remove the two thumb screws on top of the plastic pump cover Lift off the plastic cover and set it aside Locate the pump cartridges shipped with the unit and remove the cartridges from the pump by pressing down the levers on the right side and lifting the cartridges up and away from the pump Loosely fit the peristaltic pump tubing along the inside groove of the first cartridge locking the tubing clips on
34. for each of the above options depends on I gt the length and diameter of the tubing Do not allow the cleaning solution to reach the dissolution apparatus Once the pump reverses the cleaning solution is expelled into the rinse reservoir Step 5 At the time set for the clean frequency see option 1 Clean Frequency above the VK 8020 drive unit moves to the media rinse reservoir and lowers the needles The Peristaltic Pump draws the cleaning solution into the needles and sample lines for the length of time defined as the drawing media time see option 2 Drawing Media Time above The valves close and the pump pushes the cleaning solution into the return lines for the programmed push forward time see option 3 Push Forward Time above Ensure the length of time entered for the push forward time does not push the cleaning solution far enough to reach the dissolution apparatus or sample contamination can occur The Peristaltic Pump reverses and the cleaning solution is pulled back through the supply lines The pump reverses again the valves open and the cleaning solution is expelled into the rinse reservoir The VK 8020 activates the valves several times to clean the valves and needles Varian Inc Page 60 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Cleaning Tips for the VK 8020 We suggest the following tips to clean and maintai
35. g your vk 8020 19 v volumetric calibration 67 w warranty 73 warranty limitations 74 Varian Inc ap ms VARIAN Tell Us How We Are Doing We listen to our customers We work hard to make our technical documentation user friendly and to make the information in our manuals easy to retrieve and use We d like you to tell us the kinds of additional information you d find helpful in our documentation Your feedback will be carefully considered when we prepare future editions of this manual This manual should contain the following additional information The most useful thing about this book is This manual would be more helpful if My general impressions of this book are May we contact you regarding your comments YES NO If yes please write your name address and telephone number here Please return this form via mail to Technical Writing Dissolution Systems Varian Inc 13000 Weston Parkway Cary North Carolina 27513 2250 USA Optionally you can return this form via fax at 1 919 677 1550 Always feel free to telephone us to discuss your comments at 1 800 229 1108 VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual 70 9028 Revision E
36. h program The values for option 6 Set Parameters are global meaning they pertain to all programs and manual actions See Injection Option 6 Set Parameters on page 39 Injection Option 3 Inject Standards From the HPLC Injection Options screen select option 3 Inject Standards to specify the regularity of standard injections during a sequence choose sample rows to be analyzed and specify placement of standard solutions The Inject Standards screen displays INJECT STANDARDS 1 BEFORE 2 AFTER 3 BRACKET 4 NONE TIME 06 05 03 00 00 00 ELAPSE 000 00 00 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 39 P N 70 9028 Operator s Manual Operation Following is a description of the Inject Standards screen options Option Function 1 Before Select option 1 Before to inject the standard for each row of samples before the samples in each row are injected 2 After Select option 2 After to inject the standard for each row after the samples in each row have been injected 3 Bracket Select option 3 Bracket to inject the standard for each row before and after each row of samples has been injected 4 None Select option 4 None to not inject any standards Placement of Standard Vials The two rows at the front of the sample tray are reserved for standards Row S1 holds standards to be injected before each row and row S2 holds
37. ich can cause death serious injury to personnel or severe damage to equipment and property Information on safety practices is provided with your instrument and operation manuals Before using your instrument or accessories you must thoroughly read these safety practices Observe all relevant safety practices at all times Varian Inc Page 10 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Safety Practices and Hazards Operator s Manual P N 70 9028 Electrical Hazards The VK 8020 contains electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious injury or painful electric shock Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Varian trained Varian qualified or Varian authorized service engineers Consult the manuals or product labels supplied with the VK 8020 to determine which parts are operator accessible Application of the wrong supply voltage connection of the instrument to an incorrectly wired supply outlet or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment Always use a three wire outlet with ground connection which is adequately rated for the load The installation must comply with local state and fede
38. ing the needle manifold jogs up and down while opening and closing the valves This motion rinses the needles in the VK 8020 rinse tank Then the system purges the water from the sample lines The following figure is a generalized flow of the clean cycle see Figure 6 The Cleaning Process below You should use this function often FIGURE 6 The Cleaning Process Start Place Probes Move F A Open Close in Rinse Prime Tubing Head to Rinse Valves Solution Position Open Close Raise Lower Purge Tubing Reve amp Valves Needles Clear Filters Programmed Clean To run a program which rinses the needles in a predetermined cleaning solution contained in the media rinse reservoir complete the following steps Step 1 Fill the media rinse reservoir with the cleaning solution Varian Inc Page 58 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Step 2 From the Cleaning Systems Selections screen select option 2 Enable Auto Clean Option 3 Disable Auto Clean displays on the Cleaning Systems Selections screen and option 2 changes to Program Auto Clean CLEANING SYSTEMS SELECTIONS 1 START CLEAN 2 PROGRAM AUTO CLEAN 3 DISABLE AUTO CLEAN TIME 06 05 03 ELAPSE 000 00 00 Note Select option 3 Disable Auto Clean to escape the auto clean function and return to the original Cleaning Sy
39. ing arm open when the VK 8020 is operating From the Ready screen press START PROG Enter a program number between 1 and 30 and press ENTER The Select Start Mode screen displays SELECT START MODE 1 START NOW 2 DELAYED START 3 INJECTION ONLY 4 MODIFY PROGRAM PRG 1 VOL 2 ml HPLC ON R M OFF Varian Inc Page 52 Operation VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operator s Manual P N 70 9028 Following is a description of the Select Start Mode screen options Option Function 1 Start Now 2 Delayed Start Select option 1 Start Now to immediately start the selected program A screen displays indicating the printer is documenting the start of the program If enabled the printer prints the date time and header information for the program initiated When the printout is complete MOVING TO RINSING POSITION displays When the pump lines begin to prime the time to next sample and the elapsed time of the test display Select option 2 Delayed Start Enter a start time in 24 hour format and press ENTER At any time prior to the scheduled start of the program press ESC to abort the delayed start 3 Injection Only Select option 3 Injection Only to inject vials automatically from another source either another dissolution system or a non dissolution source Place the vials into the sample tray and specify the first and last rows The VK 8020 beg
40. ins injecting the samples and standards according to the parameters set in the previous section HPLC Injection Options 4 Modify Program Select option 4 Modify Program to modify or review the selected program The Program Variables screen displays see the Program Variables screen on page 48 The seven program variable options can be viewed or changed Once the program is verified or changed to the correct configuration press ESC to return to the Select Start Mode screen Select the desired start mode to start the program Press ESC twice to abort the complete operation and return to the Main Menu Manual Sample Press MANUAL SAMPLE The Manual Sampling screen displays MANUAL SAMPLING 1 START 2 SET ROW 3 SET VOLUME 4 PRIME TIME 5PURGE TIME 6 R M OPTION ROW 4 VOLUME 5mi PRIME 60 PURGE 60 Varian Inc Revision E 11 10 P N 70 9028 VK 8020 Sample Collector HPLC Autoinjector Station Page 53 Operator s Manual Operation The Manual Sampling screen options are specific to manual sampling and are independent of the stored program parameters for purge time prime time volume etc Following is a description of the Manual Sampling screen options Option Function 1 Start Select option 1 Start The following options display e Option 1 Start with HPLC begins the sequence by priming the sample lines Next the appropriate volume of sample is pulled followed by purging of the s
41. ion required to fill the loop time automatically extends until the loop is completely filled For example with an inject delay of 20 seconds the valve moves to the Inject position 20 seconds after the syringe begins to draw sample into the loop 6 Load Delay Select option 6 Load Delay to specify the duration between the injection point and the valve returning to the Load position After injecting the valve remains in the Inject position for most of the run This ensures all sample is swept from the loop and minimizes carryover However at some point before the next injection the valve must return to the load position so the loop can be filled again The valve should return to the Load position just prior to the end of the run leaving enough time for the loop to be filled before the next injection The delay interval can be specified from 00 00 immediate return to 99 59 minutes 7 Filter Change Select option 7 Filter Change The Filter Change Function screen displays Ensure option 2 disable is selected Note An asterisk indicates the current setting Varian Inc Page 42 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 After all parameters have been set press ESC to return to the Main Menu Main Menu Option 6 Injection Control Most of the VK 8020 components such as the syringe pump and injector valve can be operated m
42. is discussed below Press ESC to return to the Main Menu MANUAL OPERATIONS 1 LIFT VALVES 2 LOWER VALVES 3 OPEN CLOSE VALVES 4 GOTO ROWS 5 TURN ON R M PUMP 6 R M PUMP OFF Following is a description of the Manual Operations screen options Option Function 1 Lift Valves Select option 1 Lift Valves to raise the needles from the lowered position 2 Lower Valves Select option 2 Lower Valves to lower the needles from the raised position 3 Open Close Valves Select Option 3 Open Close Valves to open or close the valves 4 Goto Rows Select option 4 Goto Rows Enter a row number The dispensing arm moves to the specified row and stops 5 Turn On R M Pump Select option 5 Turn On R M Pump to enable the replacement media pump if installed 6 R M Pump Off Select option 6 R M Pump Off to disable the replacement media pump if installed Varian Inc Page 46 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Report Center Printer Operation and Communications From the Main Menu press ESC The Ready screen displays Press PRINT on the keypad PRINT is used to print batch information and sample times for each of the 30 stored programs to control the Report Center Printer if one is connected and to set the communication port identification number Enter the desired program number and press ENTER The Print Se
43. lections screen displays PRINT SELECTIONS 1 BATCH INFORMATION 2 SAMPLE TIMES 3 TURN ON OFF REP CENTER 4 CHANGE TO REMOTE LOCAL 5 SET COM PORT ID 6 SET BAUD RATE Following is a description of the Print Selections screen options Option Function 1 Batch Information Select option 1 Batch Information to immediately print the previously entered batch information if any for the selected program The batch information is the header information that can be entered via option 4 of the Program Variables screen See 4 Headers on page 49 2 Sample Times Select option 2 Sample Times to immediately print the sampling times for each row of the selected program This is the complete listing of all timepoints entered via option 2 of the Program Variables screen See Program Variables Option 2 Sample Time Points on page 50 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 47 P N 70 9028 Operator s Manual Operation Option Function 3 Turn On Off Rep Select option 3 Turn On Off Rep Center to turn on or off the Center Report Center Printer If the Report Center Printer is on it is turned off when you select this option If it is off it is turned on This option always reflects the opposite of the current state of the Report Center For example if it is currently disabled then TURN ON REP CENTER displays Note the state of the Rep
44. llation on page 28 Sample is not injected Pump tubing is not connected to position 3 Sample tubing is not connected to position 6 Sipper needle height is incorrect HPLC option is disabled Incorrect fill volume set Connect the pump tubing to position 3 Connect the sample tubing to position 6 Set the sipper needle to the correct height See Sipper Needle Installation on page 28 Enable the HPLC option See 1 Disable Enable Injection on page 37 Set the correct fill volume See 1 Fill Volume on page 40 Varian Inc Page 72 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 This page was intentionally left blank except for this message Varian Inc Chapter 6 Service and Warranty The warranty is provided by Varian Inc or one of its authorized representatives Service and Warranty Information Varian dissolution products carry a one year warranty on parts and labor The Dissolution Systems Service Department or one of its representatives will at its option either repair or replace any mechanical and electrical components in your instrument which prove to be defective During the first year of warranty coverage there is no charge for the labor to repair your unit The Dissolution Systems Service Department or one of its representatives will determine the best si
45. n your VK 8020 e The cannulas and needles on your VK 8020 are constructed of 316 stainless steel Although stainless steel is practically immune to rust and corrosion these can still occur if the equipment is not properly cleaned after each use e Be sure to rinse cannulas and remove the filters after each use e Although purified water is generally used to rinse the system warm water may be used to dissolve buffer salts Weak alcohol solutions of less than 25 can also be used e Avoid the use of strong organic solvents which would damage the rinse tray Report Center Impact Printer The following is helpful information for using your impact printer Installing the Cartridge Ribbon If the printer is used infrequently the print impression sometimes becomes weak because the ribbon dries out If the printed material is difficult to read and you suspect this is the cause of the problem advance to a new section of the ribbon by pressing the printer toggle switch to the Paper feed position If the printing is still faint replace the cartridge To install the cartridge Step 1 Toggle the printer off line by pressing the printer toggle switch to the OnLine Off Line position When the printer is off line the Ready LED does not illuminate Step 2 Four small grooves are embossed on the printer cover Gently push on these grooves to tilt the cover When the printer cover is tilted up you can lift it off completely Varian Inc
46. nction L LOWER NEEDLE R RAISE NEEDLE Lower or raise the sipper needle on demand by selecting L or R from the HPLC Module Control screen If the needle is currently in the raised position L LOWER NEEDLE displays If the needle is currently in the lowered position R RAISE NEEDLE displays Syringe Control For manual control of the syringe pump complete the following steps Step 1 From the HPLC Module Control screen press S Syringe Control The following screen displays 1 RIGHT SIDE 2 LEFT SIDE TIME 06 05 03 14 45 41 ELAPSE 000 00 00 SELECT VALVE DIRECTION Step 2 Select option 1 to pull from or dispense to the right toward the rinse reservoir or option 2 to pull from or dispense to the left toward the injection valve The following screen displays 1 FILL 2 DISPENSE TIME 06 05 03 14 45 41 ELAPSE 000 00 00 SELECT SYRINGE WORKING MODE Step 3 Select option 1 to fill the syringe or option 2 to dispense from the syringe Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 45 P N 70 9028 Operator s Manual Operation Main Menu Option 7 Manual Operation From the Main Menu select option 7 Manual Operation to display six options that can be used to check the functionality of the VK 8020 or to duplicate programmed operations manually All functions are executed immediately without pressing ENTER Each option
47. nd lower the sampling cannulas to immerse the filters in the liquid Step 12 Press CAL Step 13 Select option 1 Manual Step 14 Press OPEN VALVES The following screen displays PRESS lt OPEN VALVES gt TO START PRESS AGAIN WHEN 10 ML IS REACHED MANUAL CALIBRATION OF VALVE 1 TIME 06 05 03 21 56 40 ELAPSE 000 00 00 Step 15 Observe the first calibration tube and press OPEN VALVES again when 10 mL is reached Step 16 Repeat steps 14 and 15 for the remaining calibration tubes Step 17 The calibration completion alarm if enabled sounds when the calibration sequence is finished Step 18 Press MENU Step 19 Select option 7 Manual Operation Step 20 Select option 4 Goto Rows Varian Inc Page 70 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Step 21 Enter 4 to go to row 4 Step 22 Remove and clean the autocalibration block and tubes Step 23 Reconnect the top and bottom portions of the autocalibration block and place them in the rear most position between the metal brackets Step 24 Replace the media rinse reservoir in front of the autocalibration block Note If you press ESC before all the valves are calibrated you cancel Ca the calibration and return to the previously saved values for each valve None of the new calibration results are saved The calibration must be allowed to finish before the new calibration
48. needle so the needle can be moved up or down with the support carriage Step 7 Press L Lower Needle and adjust the needle height so it is approximately one to two millimeters from the bottom of a glass vial Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 29 P N 70 9028 Operator s Manual Setting Up the VK 8020 Step 8 Raise the needle support carriage and firmly secure the nut around the sipper needle Do not to over tighten the nut Raise and lower the needle support carriage repeatedly to ensure the sipper needle holds its position Hidden Key Functions To access the hidden key functions enter the following key sequences Key Sequence Function MENU gt 0 gt 0 Select 6 7 or 8 valves MENU gt 0 gt A Select standard or minimum drop volume before sampling MENU gt 0 gt B Enable or disable the beeper for moving alert MENU gt 0 gt E Select Impact or Thermal printer Note ensure this option indicates Impact Varian Inc Page 30 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Setting Up the VK 8020 Operator s Manual P N 70 9028 This page was intentionally left blank except for this message Varian Inc Chapter 4 Operating the VK 8020 VK 8020 Keypad Options The keypad on the VK 8020 is similar to a personal computer keyboard For example it has a SPACE key an ENTER key and a SHIFT key These k
49. ort Center Printer is not unique to an individual program It is global When you enable or disable it the Report Center Printer stays on or off until you change it again even if you switch programs 4 Change to Remote Select option 4 Change to Remote Local to select either the Local built in Report Center Printer local or a remote printer if one is connected This option reflects the opposite of the current state For example if it is currently set to the local printer CHANGE TO REMOTE displays 5 Set Com Port ID Select option 5 Set Com Port ID to enter a new communication port identification number The system default is communication port 01 The communication port identification number can be a value from 1 to 99 The communication port identification number is used when multiple units are linked together in a network so that the controlling computer can address individual units 6 Set Baud Rate Select option 6 Set Baud Rate The Com Port Baud Rate Selection screen displays Select the desired baud rate and press ENTER The recommended baud rate for the VK 8020 is 9600 baud option 4 Note an asterisk indicates the current setting Varian Inc Page 48 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Programming the VK 8020 From the Ready screen press PROG Enter a program number and press ENTER The Program Variabl
50. ould be set to a value large enough to completely fill the sample lines with liquid Varian Inc Page 54 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Option Function 5 Purge Time Select option 5 Purge Time Enter a duration between 1 and 99 seconds that the pump will run in the reverse direction with the valves in the bypass position and press ENTER The time should be set to a value large enough to completely empty the liquid in the sample lines back into the vessels The volume in the lines is not part of the sample volume 6 R M Option Select option 6 R M Option An asterisk indicates the current setting Ensure the asterisk displays next to option 1 Disable if the media replacement option is not installed Varian Inc chapters Maintenance and Troubleshooting General Maintenance Warning The VK 8020 and accessories contain electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can cause death serious injury or painful electric shock Perform the following maintenance when necessary Varian Inc Page 56 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Clean System Function Use the clean system function often to keep the valves
51. ples must be checked or removed for immediate testing To activate the row sampling completion alarm complete the following steps Step 1 Select option 2 Row Samp Completion Step 2 Select option 1 to enable or select option 2 to disable the alarm Step 3 Press any key to silence the alarm after it sounds Varian Inc Page 36 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Option Function 3 Prg Completion This alarm beeps at the end of the program to alert you that the test is finished It is a useful reminder that the program is complete The alarm must be acknowledged and can be deactivated by pressing any key To activate the program completion alarm complete the following steps Step 1 Select option 3 Prg Completion Step 2 Select option 1 to enable or select option 2 to disable the alarm Step 3 Press any key to silence the alarm after it sounds 4 Calib Completion The alarm beeps when autocalibration is complete and all lines are purged To activate the calibration completion alarm complete the following steps Step 1 Select option 4 Calib Completion Step 2 Select option 1 to enable or select option 2 to disable the alarm Step 3 Press any key to silence the alarm after it sounds Main Menu Option 3 Injection Options Select option 3 Injct Options from the Main Menu The HPLC Injection
52. plete the following steps Step 1 Step 2 Step 3 Step 4 Ensure the correct prime and purge times have been entered via the Main Menu options 4 and 5 Manual Prime Time and Manual Purge Time screens respectively Ensure the supplied autocalibration block is firmly in place on top of the autocalibration block and connected to the rear panel connector marked AUTO CALIBRATION From the Ready screen press CAL The dispensing arm moves forward to allow you to check the autocalibration block Fill the cleaning tray with tap water and lower the sampling cannulas to immerse the filters in the liquid The system monitor displays a reminder to ensure the tubing and pump are ready The Calibrate Base Station screen displays CALIBRATE BASE STATION CHECK PUMP amp TUBING IF AUTO CALIBRATION INSTALL CALIB FIXTURE BEFORE STARTING 1 MANUAL 2 AUTOMATIC Varian Inc Page 68 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Step 5 Select option 2 Automatic The dispensing arm moves over the autocalibration block and primes the lines according to the manual prime settings At the end of the priming cycle all valves lower and open simultaneously and begin to fill the autocalibration block As the 10 mL point is reached in each well the valve closes and the time value is recorded in memory When the last valve clos
53. pt what is stated on this line of the menu item Each time the switch is pressed another part of the menu prints Allow the printer to finish printing before pressing the switch again See the table of commands on the following page Note The printout is easier to read if the printer cover is removed Varian Inc VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 P N 70 9028 Station Operator s Manual Page 65 Maintenance and Troubleshooting SETUP MENU CONFIGURE CUSTOM CUSTOM MENU PRINT CUSTOM SETUP NEXT OK Press NEXT NEXT OK Press NEXT to avoid configuration NEXT OK Press OK to enter custom mode AUTO SEQ NO NEXT OK Press OK ZERO NEXT OK Press OK POUND SIGN NEXT OK Press OK _ UNDERSCORE NEXT OK Press OK ONLINE OFFLINE YES NEXT OK Press OK EXT CH SET NO NEXT OK Press OK PRINT READY YES NEXT OK Press NEXT PRINT READY NO NEXT OK Press OK READY Your printer is now configured correctly Varian Inc Page 66 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Fuse Replacement The fuse is located in the left section of the female line cord socket on the rear of the VK 8020 Complete the following steps in order to replace the fuse Step 1 Step 2 Step 3 Step 4 Step 5 Step 6
54. ral safety regulations Do not connect the instrument to the main power supply until you have made sure that the operating voltage is correctly set for the main power supply in the specific outlet in your laboratory to which the equipment will be connected Other Other specific warnings and cautions appear in the manuals where appropriate and detail the specific hazard describe how to avoid it and specify the possible consequences of not heading the warning or caution Warning A Warning message appears in the manual when failure to observe instructions or precautions could result in death or injury Symbols depicting the nature of the specific hazard are also placed alongside warnings Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 11 P N 70 9028 Operator s Manual Safety Practices and Hazards These symbols are also used on warning labels attached to the instrument When you see one of these symbols you must refer to the relevant operation or service manual for the correct procedure referred to by that warning label The meaning of these symbols is as follows Electrical shock Pinch point Caution Refer to accompanying documents Read all warnings and cautions carefully and observe them at all times Varian Inc Page 12 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Safety Practices and Hazards Operator s Manual P N 70 9028 Ca
55. require several The nature of the compounds and the sensitivity of the HPLC assay determine this value Varian Inc Revision E 11 10 P N 70 9028 VK 8020 Sample Collector HPLC Autoinjector Station Page 41 Operator s Manual Operation Option Function 3 Fill Speed Select option 3 Fill Speed to enter the filling speed Enter the time for a full syringe stroke from 4 seconds fastest to 99 seconds slowest This time value is used for all operations which involve filling the syringe A withdrawal rate that is too rapid can cause outgassing in the syringe or can create excessive back pressure when trying to pull viscous materials On the other hand a rate that is too slow adds to the cycle time for each injection 4 Dispense Speed Select option 4 Dispense Speed to specify the dispensing speed Enter a dispense speed between 4 seconds fastest and 99 seconds slowest As with fill soeed you may want to vary the dispensing speed of the syringe Viscous materials for example could require a slower dispensing speed than less viscous ones 5 Inject Delay Select option 5 Inject Delay to specify a delay in seconds before the valve moves to the Inject position The delay is the amount of time elapsed between when the syringe starts filling and the valve moves to the Inject position Enter a value between 0 seconds no delay and 99 seconds lf the entered delay time is less than the durat
56. roduct e the documentation package that accompanies the product containing a copy of the declaration of conformity This declaration is the legal declaration by Varian Inc that the product complies with the directives and also shows the EN standards to which the product was tested to demonstrate compliance The declaration of conformity is signed by the representative of the manufacturing plant cTUVus U S and Canadian Product Approvals The VK 8020 has been designed to comply with North American safety requirements These products have been tested and certified for the North American market by TUV Rheinland of North America Inc The TUVus mark signifies that these products have been tested to U S standards and certified for the U S market The cTUV mark signifies that these products have been tested to Canadian standards and certified for the Canadian market When the two marks are coupled the cTUVus mark signifies that these products have been tested to standards for both markets Varian Inc Page 14 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Safety Practices and Hazards Operator s Manual P N 70 9028 WEEE Directive All Varian products that are subject to the WEEE directive shipped after August 13 2005 are compliant with the WEEE marking requirements Such products are marked with the crossed out wheelie bin WEEE symbol shown on page 12 in accordance with European Standard EN 50419 This
57. s a description of the HPLC Module Control screen options Option Function 1 6 VIAL 1 TO 6 Enter the vial number between 1 and 6 to be injected The sipper needle moves left or right to the appropriate position Note it is not necessary to specify a vial position unless you want to make an injection S SYRINGE For manual control of the syringe pump see Syringe Control CONTROL on page 44 V INJ VALVE For manual operation of the injection valve press V The Operate Injection Valve screen displays Select option 1 Load Position to move the injection valve to the loading position Optionally select option 2 Injection Position to move the injection valve to the injecting position INJECT Press I to begin a single injection cycle P PRIMING Before starting a series of injections prime the sample flow path Fill the rinse bottle with ultrapure water or the selected rinse medium From the HPLC Module Control screen press P to begin the priming cycle using the parameter values speed volume and number of cycles entered earlier F FILTER CHANGER No operator input required Note the filter changer option does not display if the filter changer is disabled under the HPLC set parameters See 7 Filter Change on page 41 Varian Inc Page 44 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Operation Operator s Manual P N 70 9028 Option Fu
58. stems Selections screen Select option 1 Start Clean from either screen to escape the automatic clean function and immediately start the cleaning process Step 3 Select option 2 Program Auto Clean The Program Auto Clean Variables screen displays PROGRAM AUTO CLEAN VARIABLES 1 CLEAN FREQUENCY 2 DRAWING MEDIA TIME 3 PUSH FORWARD TIME TIME 06 05 03 08 28 46 ELAPSE 000 00 00 Note I gt The value entered for the clean frequency should be smaller than the sample timepoint Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Page 59 P N 70 9028 Station Operator s Manual Maintenance and Troubleshooting Step 4 Select each option and enter the appropriate parameters as necessary due to tubing length and diameter Option Function 1 Clean Select option 1 Clean Frequency Enter an interval of up to 99 Frequency minutes The cleaning begins at this time Note Ensure the clean frequency is set earlier than the first sample timepoint 2 Drawing Select option 2 Drawing Media Time Enter an interval of up to Media Time 999 seconds to control the length of time the cleaning solution is drawn from the media rinse reservoir 3 Push Forward Select option 3 Push Forward Time Enter an interval of up to 999 Time seconds to indicate the length of time the solution is moved through the return lines Note Appropriate parameters
59. taltic pump 20 clean frequency 59 cleaning tips 60 connecting the vk 8020 autosampler to hplc 23 conventions 18 d dispense speed 41 drawing media time 59 dual voltage option 24 e elapsed timer 35 exclusions 74 exclusive remedies 75 f fill speed 41 fill volume 40 filter change 41 flush volume 40 fuse replacement 66 g general maintenance 55 goto rows 45 h hazards 9 i inject delay 41 injection control 42 installing the printer cartridge ribbon 60 introduction 15 l lift valves 45 limitations 74 load delay 41 lower valves 45 Varian Inc Index m main menu 33 manual calibration 68 manual operation 45 media rinse reservoir installing 27 o obtaining warranty service 74 open close valves 45 p peristaltic pump setting up 20 prime time 53 print key 46 printer configuration 64 printer on off 47 printer self test 63 printer toggling online 63 program 48 program completion 36 program variable screen 52 purge time 54 push forward time 59 r ready screen 24 replacement media option 50 report center printer 46 r m pump off 45 row sample completion 35 s safety practices 9 sample time points 50 sample tray installing 26 screens initial status 24 ready 25 system monitor 24 self test printer 63 service 73 set alarms 35 setting up the vk 8020 19 start clean 57 start mode 51 syringe 44 t tips 60 troubleshooting 71 turn on r m pump 45 u unpackin
60. tch in the Paper feed position Turn on the VK 8020 Hold the printer toggle switch until printing begins The printer prints a list of the current configuration settings and performs a continuous print test Press the printer toggle switch to the OnLine Off Line position to stop the printing operation The printer is ready to resume normal operation Varian Inc Page 64 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Printer Configuration Note The printer configuration is set by the factory This procedure a should be performed only if the printer displays erroneous characters Contact the Dissolution Systems Service Department for assistance if necessary Step 3 Step 4 Turn off the VK 8020 Press and hold the printer toggle switch in the OnLine Off Line position while turning on the instrument Hold the printer toggle switch in the OnLine Off Line position for six seconds after the instrument is turned on then release the switch The printer should print SETUP MENU and CONFIGURE NEXT OK If this message does not print repeat steps 1 through 3 The printer toggle switch is used to complete the configuration Pressing the left side of the printer toggle switch selects NEXT to advance to the next menu item Pressing the right side of the printer toggle switch selects OK to acce
61. tch to Paper feed to advance the paper approximately one inch beyond the paper cutter Using scissors cut the paper feeding to the printer and remove the paper roll Varian Inc Page 62 VK 8020 Sample Collector HPLC Autoinjector Revision E 11 10 Maintenance and Troubleshooting Station Operator s Manual P N 70 9028 Step 5 Pull the remaining paper through the printer mechanism Pull the paper from the front paper cutter side Pulling the paper out of the back of the printer will damage the print mechanism Step 6 Unroll several inches of paper on the new roll Step 7 If it is jagged cut a straight edge on the paper roll to facilitate the entry of the paper into the printer Step 8 Slide the paper through the slot connecting the paper compartment and the printer compartment You can slide it in approximately 1 4 inch before it stops Step 9 While holding the paper in place press the printer toggle switch to the Paper feed position and hold until approximately one inch of paper has emerged from the top of the printer Make sure the roll of paper feeds squarely If it does not the paper will jam and possibly damage the printer mechanism Step 10 Release the printer toggle switch Step 11 Turn the paper roll to take up any slack in the paper feeding to the printer Step 12 Place the paper roll into the paper compartment Step 13 Replace the paper roll cover If the cover is difficult to remove or replace the left
62. te to repair the unit either onsite or returned to Varian Inc Any onsite warranty services are provided only at the initial installation point Installation and onsite warranty services are available only in Dissolution Systems service travel areas Varian Inc Page 74 VK 8020 Sample Collector HPLC Autoinjector Station Revision E 11 10 Service and Warranty Operator s Manual P N 70 9028 Exclusions and Limitations Excluded from this warranty are expendable or consumable items such as but not limited to paddles baskets vessels and acrylic water baths Also excluded are defects from improper or inadequate maintenance by the customer user induced chemical action or contamination unauthorized modification or misuse and improper site preparation and maintenance Operation of software is not warranted to be uninterrupted or error free Obtaining Warranty Service To obtain warranty service in the United States contact the Dissolution Systems Service Department at 800 229 1108 to obtain authorization to return units for repair At the option of the customer onsite warranty service is available but travel charges may be incurred The customer should prepay all shipping charges for products returned to the Dissolution Systems Service Department unless otherwise authorized and Varian Inc will pay all charges for return to the customer Warranty Limitations Varian Inc makes no other warranty either express or implied
63. the Cartridge Ribbon 60 Replacing the Paper Roll 61 Toggling Your Printer Online 63 Printer Self Test 63 Printer Configuration 64 Fuse Replacement 66 Volumetric Calibration 67 Automatic Calibration 67 Manual Calibration 68 Troubleshooting 71 Chapter 6 Service and Warranty 73 Service and Warranty Information 73 Exclusions and Limitations 74 Obtaining Warranty Service 74 Warranty Limitations 74 Exclusive Remedies 75 Index 77 Tell Us How We Are Doing 79 Varian Inc Table of Contents This page was intentionally left blank except for this message Varian Inc List of Figures FIGURE 1 FIGURE 2 FIGURE 3 FIGURE 4 FIGURE 5 FIGURE 6 VK 8020 Cable Connections 21 Connecting the Autosampler to the HPLC 23 Initial Status Screen 25 Ready Screen 25 VK 8020 Keypad 31 The Cleaning Process 57 Varian Inc Table of Contents This page was intentionally left blank except for this message Varian Inc chapter1 Safety Practices and Hazards The VK 8020 has been carefully designed so that when used properly you have an accurate fast flexible and safe instrument If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Operation of a VK 8020 involves the use of aqueous liquids Unskilled improper or careless use of this instrument can create shock hazards fire hazards or other hazards wh
64. to set up the autoinjector station See Main Menu Option 3 Injection Options on page 36 4 Manual Prime Time Use this option to set the interval the peristaltic pump runs forward drawing medium into the system before the valves open to deliver samples Select option 4 Manual Prime Time Enter a value for the priming time from 1 to 99 seconds and press ENTER The Main Menu displays Note at a minimum the manual prime time should be long enough for medium to be seen dripping from the return cannulas In general the longer the tubing lengths the longer the priming time required This value is not used by sampling programs which have their own individual priming time values It is used only with the MANUAL SAMPLE CAL and CLEAN SYSTEM keys on the VK 8020 5 Manual Purge Time 6 Injection Control Use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn Select option 5 Manual Purge Time Enter a value for the manual purge time from 1 to 99 seconds and press ENTER The Main Menu displays Note purging also clears the sampling filters of particulate matter that may restrict successive samples As with priming the longer the tubing lengths the longer the purge time required This value is not used by sampling programs which have their own individual purging time values It is used only with the MANUAL SAMPLE
65. unction 4 Headers Select option 4 Headers The Batch Selections screen displays six options operator name product name batch number lot number group number and notation Each selection on this screen accepts 19 characters Characters can be letters numbers special characters or spaces The information entered for each selection prints in the header of each printout The entries made from this screen are attached to the active program Since there are 30 programs there can be a different set of entries for each All are stored in the battery protected memory and remain until changed via this screen Note the Report Center Printer is limited to ten characters for each entry Characters 11 19 are kept in memory and printed to the remote printer port and shown on the display 5 Prime Time Select option 5 Prime Time The Prime Time screen displays Enter a value for the priming time from 1 to 99 seconds and press ENTER The priming time is the interval the Peristaltic Pump runs forward drawing media into the system before the valves open to deliver samples This ensures a continuous liquid head in all lines The priming time should be long enough to completely fill the sampling lines with liquid At a minimum it should be long enough for liquid to be seen dripping from the return probes In general the longer the tubing from the sampling probes to the valves the longer the priming time required Note prime time is unique to e
66. ution A Caution message appears in the manual when failure to observe instructions could result in damage to equipment Varian supplied and or other associated equipment A Note appears in the manual to give advice or information G gt Information Symbols Switches main power on Switches main power off Indicates single phase alternating current ONS Indicates the product complies with the requirements of one or more European Union EU directives _ Indicates specific equipment meets consensus based standards MD of safety to provide assurance required by OSHA that these NE in products are safe for use in the workplace for North America Indicates that this product must not be disposed of as unsorted municipal waste see WEEE Directive on page 14 Varian Inc Revision E 11 10 VK 8020 Sample Collector HPLC Autoinjector Station Page 13 P N 70 9028 Operator s Manual Safety Practices and Hazards General CE Compliant Products The VK 8020 has been designed to comply with the requirements of the Electro magnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive LVD of the EU Varian Inc has confirmed that each product complies with the relevant directives by testing a prototype against the prescribed European Norm EN standards Proof that a product complies with the directives is indicated by e the CE marking appearing on the rear of the p
67. values are saved Step 25 Step 26 Press H to return to the home position Press ESC three times to return to the Ready screen Varian Inc Revision E 11 10 P N 70 9028 VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual Page 71 Maintenance and Troubleshooting Troubleshooting The Dissolution Systems Service Department can assist you if you experience problems or have questions concerning your VK 8020 Many problems can be traced to simple sources and are easily solved Following is a troubleshooting guide which may help you The Dissolution Systems Service Department can be reached at 800 229 1108 inside the US or 919 677 1108 outside the US Optionally you can send a fax to 919 677 1138 You can also e mail the Dissolution Systems Service Department at dissolution service varianinc com Problem Possible Cause Possible Solution HPLC does not inject HPLC is disabled Improper connection to the HPLC Improper inject time interval entered Enable the HPLC Ensure the connections to the HPLC are correct Enter a proper inject time interval See 2 Inject Interval on page 37 Active peak is smaller than expected Injector loop is not filled completely Sipper needle height is incorrect Ensure the injector loop fills completely See 1 Fill Volume on page 40 Set the sipper needle to the correct height See Sipper Needle Insta

Agilent Technologies VK 8020 Sample Collector HPLC Autoinjector Station Operator s Manual

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