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Leica CM3050 S Cryostat Manual

1. ini 15 DSA Maintenant Eorann aaa 15 3 Installato mmc 16 gl SitereguirenenSas anice 16 Fo Pe Generalsite frequente a i ee ee 16 D162 ELC CURIE alll onne Cion ee O 16 3 2 UMP ACKING ANG IN SLAN AU OM areni a 16 53 LANG ALC EIIVE FY annie 17 4 Instruction Manual V1 1 02 2000 Table of Contents 34 Installimg imserting aGGESSOnNEes acc 18 3 4 1 Installing the handwheel c ccccsssssesssssssessssseecsessecesessesecseseeseseeseuseseesacsesenseusassusensessesersansatsesaneases 18 3 4 2 NSering TNC accessories 18 343 EVE TO US VUE CINY antonio rilevava 19 35 PROF 0 SWITCHING onthe Instrument lecce 19 A instrument Properes oie istic ee ieee ei eee 20 Li eccelle 20 427 ccnnicali Dialisi 22 5 Uperaioi a alcalina 24 5 1 Operating the instrument for the first time cccecssescsessessssececsnssesecsesessessusesenseseesenseeseseasasensenseneas 24 52 OWENT ONIU CS nc 25 dio GONMPOLPanel Lsssratne ieri iris lie iano lire 26 Doll Key tuncions Mentro panei Tesamen a A 26 5 3 2 Display functions IN controlpanel 1 26 5 3 3 Display Indications when SWITCHING ON ii 21 5 04 Ment TUNIC TV LVS aces th teas vos cerceaaoas eseahrncaies css ca A 28 PRS D olats diS play ee ee 31 5 3 6 Indication of refrigeration state section thickness setting and counters 32 5 4 Contopane 2 siccrgersincaistyvnaryeaternauentvessausit naevus yas aah nated a a a anuatanada
2. The control keys are located on top of the con trol unit see photograph To lift the instrument press 4 To lower the instrument press As long as a button is held the instrument will go up or down until reaching the upper or lower limit 1 6 Maintenance and care Protect the lifting pistons in the four legs of the height adjustment device against dust and water Approximately every six months lubricate the legs with a thin coat of resin free oil Once every year have the height adjustment device checked by an authorized Leica ser vice engineer Leica CM 3050 S Cryostat Nominal voltage Nomi nal frequency Type of actuation Total height of stroke 230 mm 1 7 Troubleshooting 1 Slight and uniform loss of height of the de vice in the course of several weeks Is nor mal Remedy Correct the loss of height by pressing the 4 button until the instrument is lifted back up to the desired working level The cryostat is In an inclined position due to an uneven upward movement of the lifting pistons Difference of level among the four legs gt 5 mm Remedy Have the instrument travel once up and down over the entire height adjustment dis tance press 4 until the instrument reach es the upper limit position and then w until the instrument reaches the lowest possible position Then lift the instrument back to reg ular working height Should the problem re occur several times call Leica
3. 4 1 Overview Cryostat CM3050 S Control panel 1 Control panel 2 Storage shelf left Storage shelf right Rubber mat Mains switch Automatic cutout for instrument Automatic cutout o Sin LEICA osos a DDR DN sa ISS c O o for sectioning motor m CS E Footswitch port 8 Quick freeze shelf 9 Stationary heat extractor 10 Mobile heat extractor 11 Specimen disc 12 Thermoblock optional 13 Section waste tray 14 Brush shelf 15 Specimen head w o specimen cooling 16 Specimen head with specimen cooling Option 17 90 Prism instruments with specimen cooling only 18 Knife holder base 19 Knife holder CE 20 Knife holder CN 21 Knife holder CS 22 Footswitch with protective guard 20 Instruction Manual V1 1 02 2000 4 Instrument Properties Leica CM3050 S Cryostat 21 4 Instrument properties 4 2 Technical Data il Type Operating temperature range ambient temperature 18 C to 40 C All specifications related to tempe rature are valid only up to an ambient temperature of 22 C and relative air humidity of less than 60 Marks of conformity Nominal voltage Nominal frequency Power draw Max start up current for 5 sec Protective fuse Automatic cutout Pollution degree Overvoltage installation category Heat emission max Refrigeration system Cryoch
4. 1 Refrigeration state cT p810 C o T 3 5 c mjajHjojM Ej EE Meaning of the refrigeration symbols asterisks gt see chapter 5 3 5 Status indication 2 Section thickness Ci T 3 0 c olt 3 5 c LITITT TT TLT fufofifo fo The sectioning thickness Is set with the or key see chapter 5 4 7 Setting the section thickness 3 Section counter c T s o c jor 3 5 c PT TTT Tt Tt tt gt lnjojojo Once displayed via arrow keys the section counter can be set to 0 at any time e To set to 0 press yy lf the section counter Is not set to 0 by the user at some point sections are counted up to 999 at which point the counter automatically switches back to 0 32 4 Total section thickness counter CiT 3 ojetc olT 3 5 c PT tT TT LT fet ojo ojo jo Can also be set to 0 at any time via ost key lf the total section thickness indication is not set to 0 manually the instrument counts up to maximum 9999 5 um at which point the counter will automati cally switch back to 0 This indication shows the total thickness of all sections carried out since the counter was last set to 0 All sections in sectioning and trim ming mode are counted 5 Preset counter C T 3 0 ete O T 3 5 C PT tt TTT tt tt a nof 5 e Setting preset counter to 0 Press Gy key can als
5. 100m in10 um steps Motorized trimming 100 300m in50 ym steps e Select Continuous stroke in functional area A e Activate trimming by pressing RUN STOP and RUN ENABLE located in functional area C simultaneously or by depressing the footswitch e To stop trimming press RUN STOP or RUN ENABLE or depress the footswitch again Trimming thickness can be changed while trim ming is In motion by pressing or 38 Instruction Manual V1 1 02 2000 5 Operation 5 5 Daily operation of the instrument A Select chamber temperature and in instru ments with specimen cooling specimen temperature according to the type of speci men to be sectioned see also chapter 6 3 Reference chart for temperature settings Do not continue with step 5 5 1 before the actual chamber specimen temperature corresponds to the set value This may take several hours e g when operating the in strument for the first time or when the instru ment had been switched off Prior to operating the instrument please read and observe the fol lowing safety instructions e Integrated safety devices Chapter 2 2 e Handling microtome knives blades Chapter 2 3 4 e Knife guard Handwheel lock Chapter 2 3 5 e Motorized sectioning Chapter 2 3 6 e Defrosting handling frozen tissue Chapter 2 3 7 e Frozen instrument parts and accessories Chapter 2 3 8 e Infectio
6. Leica CM3050 S Crvostat Instruction Manual Leica CM3050 S Cryostat English V1 1 02 2000 Always keep this manual near the instrument Read carefully prior to operating the instrument Year of Manufacture ccccccccccccccsssecesseesestreseens Country of Origin Federal Republic of Germany Issued by Leica Microsystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 6224 143 0 Fax 49 6224 143 200 Internet http www hbu de Leica CM 3050 S Cryostat Important note The Information contained in this document rep resents state of the art technology as well as the current state of knowledge Leica will not assume liability for errors that might be contained in this manual nor for acci dental damage or damage arising from the de livery performance or use of this manual Therefore no claims can be made based on the text and illustrations in this instruction manual Leica Microsystems Nussloch GmbH reserves the right to change technical specifications without notice since each of our products is subject to a policy of continuous improvement This document is protected under copyright laws Leica Microsystems Nussloch GmbH retains all rights related to this documentation Any reproduction of text and illustrations or any parts thereof in form of printing photo copying microfiches or other methods includ ing electronic systems requires the pr
7. e Standard delivery information on accessories Instrument properties e Overview Technical data Operation e Controls e Setup daily use and shut down Trouble shooting and applications tips e Operating errors e Display error messages e Temperature reference chart Disinfection cleaning and maintenance Warranty and service Electrohydraulic height adjustment Ordering information accessories EC Declaration of Conformity Alphabetical index Leica CM 3050 S Cryostat 1 Important information 1 1 Symbols used in this manual and their meaning A i 5 Fig 5 Warnings appear in a grey box and are marked by a warning triangle 1 Notes i e Important user information ap pears in a grey box and is marked by an information il symbol Figures in brackets refer to item numbers in drawings or to the drawings themselves Instrument type All information given in this instruction manual applies only to the instrument type indicated on the title page A name plate indicating the instrument serial number is attached to the back of the instru ment Required information for all inquiries For any inquiries please specify e Instrument type e Serial number 1 Important information General This Instruction manual includes Important in structions and information related to the operat ing safety and maintenance of the instrument The instruction manual
8. and the height adjustment device This applies especially to all work carried out on the hydraulic system of the height adjustment device 59 Appendix 1 4 Installation e Open the shipping box and remove all packing material including the Sty rofoam pieces e Take the height adjustment device out of the box e At the place of installation place the U frame on the floor with the open side facing yourself Adjust the U frame parallel to the wall maintaining a distance of approx 10 cm between wall and U frame e Push the ab button to lower the height adjustment device to the lowest possible position 60 AX WX AX A _ Z zz EB Z When placing the instrument onto the U frame make sure the power cord does not get caught and thus crushed between U frame and cry ostat housing In order to be able to move the Instrument use a fork wrench and unscrew the rollers on which the Leica CM3050 S moves by about 2 cm Roll the cryostat onto the U frame as far as it will go The rear of the cryostat housing has to fit closely to the rear bar of the U frame Roll the cryostat onto the U frame as far as It will go The rear of the cryostat housing has to fit closely to the rear bar of the U frame Plug the height adjustment device into the mains socket The unit is now ready for use Instruction Manual V1 1 02 2000 Appendix 1 5 Control keys
9. only CT centered is displayed Leica CM3050 S Cryostat 27 5 Operation 5 3 4 Menu functions setting parameters of refrigeration time and preset counter Once the instrument has gone through the initial sequence of indications you can go on to the menu functions to select and or change instru ment parameters If display is locked x Press key until display background lights up Next press the menu key With each key stroke the following parameters will be displayed dis play sequence as listed 1 Specimen temperature in instruments with specimen cooling only 10 50 C 2 Cryochamber temperature 0 40 C 3 Selected number of sections preset counter ON OFF and setting selection from 1 to 999 4 Maximum specimen temperature ON OFF 5 Real time in 1 minute steps 6 Defrost time starting time of automatic cryochamber defrosting in 15 minute steps 7 Duration of defrost cycle cryochamber defrosting 6 to 12 minutes duration Menu items 5 and 6 displayed on 24 hour clock 28 1 Specimen temperature instruments with specimen cooling only CN Press key until menu item Set specimen temperature is displayed SJET TIEIMIP o t c Press key briefly The line in the specimen temperature field is replaced by an asterisk SJET TEMPI o T c ASSISI i A From this point on the refrigeration sys tem is activated a
10. will be resumed Risk of injury Therefore Do not interrupt sectioning trim ming by setting the sliding potenti ometer to zero speed See also safety instruction in Chap ter 2 3 6 Motorized sectioning Vmax key As long as the button is pressed the sectioning motion Is carried out at maxi mum speed 210 mm s While Vmax Is active the sectioning window is inactive When the Vmax key is released the sectioning window becomes active again 36 5 4 4 Emergency stop e Activate by e either pressing the emergency stop button forcefully must lock in position e or depressing the footswitch force fully Either way sectioning is interrupted at once In both cases the red srop LED in functional area A lights up indicating that the emergency stop function has been activated Simultaneously all 3 sectioning modes are deactivated all 3 LEDs off e To deactivate the emergency stop function rotate the button in the direction of the ar row until it unlocks and slips upward into Its Original position As soon as the emergency stop button has been unlocked the red sr LED Is extinguished e fthe emergency stop function has been ac tivated with the footswitch unlocking Is not necessary As soon as the footswitch is released the red srop LED Is extinguished e To continue work select one of sectioning modes 1 3 and restart the sectioning motion See also chapter 2 2
11. 12 e Push microtome slightly towards the right until axle 9 fits tightly into coupling 11 PA ON unnust prne t e Loosen screws 8 with Allen key e Unlock handwheel e Lift microtome a little and pull slightly to the e Adjust the parts into each other by rotating left the handwheel slightly backward and forth e This loosens the plastic coupling 11 which e Lock handwheel again in lower position connects the two axles e Tighten screws 8 e Reconnect plugs of coarse feed motor 6 and temperature sensor 7 e Mount specimen head in reverse order as described on previous page e Insert accessories storage shelf section waste tray etc e Insert sliding window e Connect instrument to mains and switch back on e Remove the microtome from the chamber Leica CM 3050 S Cryostat 55 7 Disinfection cleaning and maintenance 7 6 Exchanging the fluorescent light lamp A lowing safety instructions e Maintenance Chapter 2 3 13 Prior to exchanging the lamp please read and observe the fol Removing the sliding window e Hold sliding window 1 at grip bar 2 lift slightly and pull out towards yourself i mi 56 Removal The lamp 3 is located behind the glare shield 4 Carefully hold lamp with both of your hands Slightly pull the lamp downward at the left side to remove it from fastening clip 5 Pull the lamp towards the left to remo
12. Technical Service The height adjustment device does not reach the maximum upper level or the instru ment lowers Itself by a relatively large dis tance gt 5 cm within a short time 1 8 Technical Data 230 240 V 100 115 V 50 Hz 60 Hz Elektrohydraulik Carrying power 200 kp Dimensions Width 880 mm without control unit 1030 mm with control unit Depth 555 mm Weigth approx 30 kg 61 Appendix 2 Ordering information accessories 2 1 Knife holders Knife holder CN for reusable microtome knives w anti roll guide adjustable knife guard and knife support bars for slim and Wide KNIVES 0419 18649 Knife support bar for knife holder CN for slim KNIVES 0419 19426 Knife support bar for knife holder CN for Wide KNIVES ou cccccccccccssscsscscsscsssscsssscsssscesescesencnseneevenensenes 0419 19427 Knife holder CE w glass anti roll guide for low profile disposable blades with lateral displacement feature 0419 30383 Knife holder CE w glass anti roll guide for high profile disposable blades With lateral displacement feature ss essescsascesesnecuseccneseasssaesinmeaayseaupamannestuaagananecnnavanaauat A 0419 30390 Knife holder CS for reusable knives with one knife support mounted optionally on the left or right anti roll guide and knife guard sssssssccsencensisrsa 0419 19787 Auxiliary clamping device for knife holder CS to be used instead of knife support for increased stability 0419 209
13. i1i Re ata eae eee ei ee ree ee Digits 3 6 indicate coarse feed position HOME STOP e Rear limit position e Front limit position Indication of malfunction or handwheel locked e Microtome plug loose implausible values etc 2227 LOCKED Indication LOCKED completely overlays digits 3 through 16 i e while LOCKED is displayed no other parameters will be displayed in digits 3 16 of the lower line e Handwheel is locked e Further indications see chapter 6 1 Error messages indicated in the display screen Digits 7 16 indicate the following parameters e Refrigeration status Digit 15 cryochamber Digit 16 specimen Meaning of refrigeration Refrigeration OFF symbols Refrigeration ON Defrosting active e Section thickness set value e Section counter actual value e Indication of total section thickness actual value e Preset counter Digits 7 16 Scroll via arrow buttons to display individual parameters 31 5 Operation 5 3 6 Indication of refrigeration state section thickness setting and counters These parameters can be displayed via the ar row keys When pressing 4 the parameters are dis played in the sequence listed on this page when pressing 4 they are displayed in re verse order The display can be scrolled contin uously i e after the last parameter the display will automatically start again with the first
14. knife as long as the key is held STOP LED located in key W Coarse feed fast forward As long as the forward coarse feed both fast and slow is in motion the STOP LED is illumi nated Upon reaching the front limit position the STOP LED starts flashing and the display in control panel 1 reads STOP 37 5 Operation Functional area B all models 5 4 6 Trimming and sectioning functions 5 4 7 Setting the section thickness Switching between trimming and sectioning e Activate sectioning mode Rm off CIT 4c fol etc tam Press this key to alternate between the S Y fpfot tol jo SECT f two functions e Press or to select section thickness mm Illuminated Pressing one of these keys once makes the Trimming mode is active display in control panel 1 switch to section A thickness indication thickness value not yet TRIM OTT i i co being altered Pressing or further Sectioning mode Is active starts to change the actual section thick ness setting Selecting trimming thickness e Activate trimming mode e Select trimming thickness sectioning thickness at which trimming is carried out 0 2pm in05 pm steps by pressing or 2 10um in 1 um steps 10 20pm in 2 um steps Selectable section thickness settings The selected thickness Is displayed in the cor responding LED 20 60um in 5 um steps 5 10 30 50 100 150 60
15. menu item Fast object O freezing is displayed FIAIS T 0 B J FURIE E 2 1 AIII oF tt FIAIS T OBJ F R E E Z I Press lock unlock key to activate or de activate ON OFF 3 5 Real time Q Press key until menu item Time is dis played Ca ns TME 1 0 2 3 PEEP TPT TT ET ey Ty Use arrow keys 4 or to set real time Setting minutes as well as hours in 1 minute steps Holding arrow button in creases scrolling speed 6 Starting time of automatic defrost cycle Press key until menu item Defrost time is fe displayed D E F R TH ME a 3 NNNNNNNNZZNZZNNA Time on display start time of defrost cycle e Use arrow keys A or to select the de sired defrost start time with 90 prism see also chapter 5 5 2 30 The automatic defrost cycle takes place once every 24 hours at the selected defrost time 7 Duration of defrost cycle Press key until menu item Defrost period is displayed DJEJF R O S T PIER TOD C H A M B E R Joja M 1 efrost keys or to adjust frost time Only if necessary see below Important information for setting the defrost parameters il Starting time of defrost cycle We recommend to program the au tomatic defrost cycle for a time out side regular working hours as a period of 4 hours should pass after the defrost cycle before w
16. roll plate dam aged Blunt knife Clean with dry cloth or brush Replace anti roll plate Use different part of knife or replace knife 4 Troubleshooting applications tips Problem Causes Remedies Sections curl on anti roll plate Anti roll plate does not pro trude far enough beyond the knife edge Readjust correctly Scraping noise during section ing stroke and specimen return stroke Anti roll plate protrudes too far beyond the knife edge and Is scraping against the specimen Readjust correctly Ondulated sections Knife damaged Edge of anti roll plate damaged Use different part of the knife or replace knife Replace anti roll plate Chatter marks form during sectioning Specimen insufficiently frozen onto specimen disc Specimen disc not clamped tightly enough Specimen holder ball joint not clamped Knife not clamped tightly enough Selected section thickness too thick specimen has come off the specimen disc Specimen very hard and inho mogeneous Blunt knife Knife profile inappropriate for the type of specimen to be sectioned Wrong clearance angle Refreeze specimen onto disc Check disc clamping Check ball joint clamping Check knife clamping Refreeze specimen onto disc Increase section thickness if possible reduce specimen surface Use different part of knife or replace knife Use knife with a different pro file
17. sectioning motor only 5 4 1 Selecting a sectioning mode start and stop sectioning Three different sectioning modes can be select ed for motorized sectioning Sectioning mode 1 Intermittent stroke The specimen head travels up and down as long as the RUN STOP and RUN ENABLE keys are pressed simulta neously or as long as the footswitch Is depressed Sectioning mode 2 Continuous stroke The specimen head travels up and down until itis stopped by pressing RUN STOP or RUN ENABLE or by depressing the footswitch Sectioning mode 3 Single stroke LT The specimen head carries out one sec tioning stroke and then stops in the low er limit position Activating a sectioning mode Press one of the three sectioning mode keys de scribed above While a sectioning mode is ac tive the LED in the corresponding key will be il luminated 34 Start stop motorized sectioning Sectioning mode 1 Intermittent stroke e Simultaneously press and hold RUN STOP and RUN ENABLE or depress and hold footswitch e To stop the sectioning stroke release the keys or the footswitch The specimen head stops immediately Sectioning mode 2 Continuous stroke e Simultaneously press and release RUN STOP and RUN ENABLE or depress and release footswitch e To stop sectioning Press and release RUN STOP or RUN EN ABLE or depress and release the footswitch The specimen head stops upon reaching the lower limit position Sec
18. 0 mm s fast 1 according to IEC 1010 UL 3101 2 according to CECOMAF Liquid temperature 45 C Evaporation temperature 25 C 23 5 Operation 5 1 24 Operating the instrument for the first time Prior to setting up the instrument make sure to read the instruction manual carefully and in full When setting up the instrument please follow the individual chapters in the following order First study chapters 1 2 and 3 install the instrument as described in chapter 3 The overview in chapter 4 1 is helpful during the unpacking and instal lation procedure in order to identify individual parts and place them in the correct location Go on to chapter 5 Read chapters 5 1 5 2 5 3 and 5 4 to learn all about the controls Next start to work with the instrument chapter 5 5 Follow the instruc tions in chapter 5 5 Chapter 5 5 2 applies to instruments with specimen cooling only The temperature chart in chapter 6 3 is useful when It comes to select ing appropriate sectioning temperatures for different types of speci mens When disinfecting cleaning the instrument and or removing rein stalling the microtome please follow the instructions in chapter 7 In case of problems and malfunctions please refer to chapters 6 1 and 6 2 These chapters list the most common types of problems which many times have simple causes that can be eliminated by the actual user If you find Call Tec
19. 3 Safety Emergency stop function for further information on the emer gency stop Instruction Manual V1 1 02 2000 5 Operation Functional area B all models 5 4 5 Coarse feed keys The four coarse feed keys in functional area B function as follows Coarse feed fast backward Travelling speed 1 000 m s e Press amp key to activate Specimen head travels backward away from knife a until it reaches the rear limit position b until it Is stopped by pressing one of the coarse feed keys to stop the function any of the four coarse feed keys can be pressed Coarse feed slow backward Travelling speed 500 um s e To start press and hold Specimen head travels backward away from knife as long as the key Is held As long as the backward coarse feed both fast and slow is in motion the HOME LED is illuminated LED HOME located in key Coarse feed fast backward Upon reaching the rear limit position the HOME LED starts flashing and the display in control panel 1 reads HOME Leica CM 3050 S Cryostat Coarse feed fast forward Travelling speed 1 000 um s e Toactivate the function press and hold The specimen head travels forward to wards the knife as long as the key is held Coarse feed slow forward Travelling speed 500 um s e To activate the function press and hold The specimen head travels forward towards the
20. 5 Specimen orientation e Select desired section thickness see chap ter 5 4 7 Setting the section thickness e Start sectioning at approx 20 um reduce section thickness gradually until reaching the appropriate thickness for the type of specimen to be sectioned e Discard the first 2 to 3 sections cut at the de sired section thickness When sectioning manually rotate the hand wheel evenly and at uniform speed For all details on motorized sectioning please refer to chapters 5 4 1 to 5 4 4 Selecting a sectioning mode Start and stop sectioning Setting the sectioning window Selecting sectioning speed Emergency stop For advice on appropriate Sectioning tempera tures for different types of tissue please refer to chapter 6 3 Reference chart for temperature settings Instruction Manual V1 1 02 2000 5 Operation 5 6 Finishing work 5 6 1 Daily routine steps Remove all specimens from the cry ochamber and store safely See also corresponding safety instruc tions in chapter 2 3 7 Defrosting handling frozen tissue e Lock handwheel e Remove knife from knife holder disinfect dry see chapter 7 Disinfection cleaning and maintenance and store in the knife case e Use a cold brush to sweep frozen sectioning waste into the section waste tray e Empty section waste tray dispose of sec tloning waste according to the applicable regulations on disposal
21. 5 seconds 2 Coarse feed Coarse feed malfunction has occurred C T 3 0 c o T 3 5 c EERE This message is also displayed if the coarse feed plug has not been reconnected properly after reinstallation of the microtome 3 Temperature indications el t o c o T 3 5 c ERR elt s o o c Jolt c PTET TT eT Pe et eT TT If the symbol Is displayed instead of chamber or specimen temperature indication the corre sponding temperature sensor is broken or a short circuit has occurred 46 4 Service C T 3 0 c o T 3 5 c sfefafvpifefe LD This message is displayed after a certain number of operating hours to remind the user to have a preven tive maintenance done by Leica Technical Service This message is displayed only if the instrument is not regularly ser viced by Leica Technical Service We recommend a maintenance contract once the warranty period has expired Contact your local Leica sales office for details If one of the error messages listed under 2 3 and 4 is displayed please contact Leica Technical Service 6 1 1 Other error indications sro red in control panel 2 is illuminated e Emergency stop button accidentally activat ed gt Unlock e Footswitch not properly connected gt Check connection connect properly if applicable Instruction Manual V1 1 02 2000 6 Troubleshooting ap
22. 71 Knife support bar for knife holder CS aren a A A 0419 25681 Knife holder base for knife holders CN CE and CS 0419 26140 Knife rest with section collecting tray for knife holder CS 0419 18996 2 2 Specimen discs POC MEM CSE 20M iii 0370 08636 Species AMI cca 0416 19275 Specimen disc 30MM darla 0370 08587 Specimen CISC 40 Mirror 0370 08637 Specimen dis 55 lalla 0419 26491 Specimen dise 50X QO MM sarcen aAA E eee 0419 26750 other sizes on request 2 2 Accessories JOEP S M eaaa ee ew O ea karte ahaa a ce al 0443 25949 Mobile EAT OX EAC LOM nsen a A e 0443 26836 THeErMODIOCK arsena a A A E A 0398 18542 Glass anti roll guide complete assy for knife holder CE ssssssssissssrsirrrsrrinsrsisrrsrsrerrsrsrrsrsrererrerrrrnrnrns 0419 30402 Glass anti roll guide upgrade kit for retrofitting knife holders CN CS for C profile KNIVES 0419 30403 2 4 Consumables OCT embedding compound for freezing tissue specimens onto specimen discs bottle a E AEA EEE O E EO Oe ee 0201 08926 Cryostat oil No 407 bottle of 100 ML 0336 06099 62 Instruction Manual V1 1 02 2000 Appendix EC Declaration of Conformity feica We herewith declare in exclusive responsibility that the Cryostat Microtome Type CM3050 S was developed designed and manufactured to conform with the Council Directive 89 392 EEC Appendix Il A Machinery Council Directive 73 23 EEC Low Voltage and Council Directive 89 336 EEC Appendix Electro
23. 9 i POSItion upper edge of specimen just below the knife edge continue to rotate the hand wheel clockwise Press sectioning window key once More Leica CM 3050 S Cryostat The value has been stored when the LED in the sectioning window key lights up briefly If the LED flashes the value has not yet been stored because the user has tried to set a win dow margin outside the possible window range the area in which the sectioning window can be set Is slightly smaller than the total section stroke e Inthatcase repeatthe step in question il The sectioning window cannot be deleted If need be it can be set to maximum size i e almost the en tire sectioning stroke will be car ried out at one and the same speed Every time after the instrument is disconnected from mains the sec tioning window is set to maximum size upon switching back on This also applies when the instrument is switched on for the first time 35 5 Operation Functional areas A and C Instruments with sectioning motor only 5 4 3 Selecting sectioning speed e Sectioning speed is selected via the sliding potentiometer Speed can be changed while sectioning Is in motion Caution when working in continuous mode If the slider is set to 0 the sectioning motion stops however the actual sectioning mode is still active just operating at 0 speed If the slider is accidentally moved sectioning
24. MINUS C 51 7 Disinfection cleaning and maintenance scscssssssssesssesssssessessessessesserseeserserseeseeeees 52 TE SREY AS UR UIC LUO NS zee sutecastesesicetecstsu ec adie A tan Sauce aasceeanacaa ora ateaaetiaaes 52 1 2 Spray disinfection with Leica CryOfect cccescscsssssescsscsessssscessrsseseeseseseesseeessesesessnseseseesessassnsenses 52 Foe Disintechon wih CONV EMUON AL sismiche 53 TA CIGNO leale ea 53 75 Removing reinstalling the MICrOtOME c cc ecssesssessecsssececsessecesseseeseseesesseseneessusassesensessnsausassnsensensas 54 TDal lt REMOVING NEMICO NE ira 54 7 5 2 Disassembling the specimen head 54 79 3 Reinstalling the MIcrotomeszacinaelalcaar anni 55 7 6 Exchanging the fluorescent light lamp 56 8 Waranty and Senicar 57 i Mardi 57 o2 Tecnnical Service MOMO N sissi 57 8 2 1 Important information for repairs returns of cryostats cccesesessesceesseeesseeesseeessnceesnsseeeseneeeenees 58 8 3 Shutdown and disposal of the INStTUMENT ia 58 Apend Lea 59 6 Instruction Manual V1 1 02 2000 The chapters of the Leica CM3050 S cryostat Instruction manual Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Appendix Structure of the manual e Table of contents e mportant information on this manual Safety e Read this chapter before operating the instrument Installation e Unpacking and installation
25. Muscular Nose Pancreatic Prostate Ovarian Rectal Skin with fat Skin without fat Spleenal or bloody tissue Testicular Thyroid Tongue Uterus curettage Please note The recommended temperature settings are based on long term experience However they can only be guidelines for general orientation as each individual specimen may require particular adjustments Leica CM3050 S Cryostat 51 7 Disinfection cleaning and maintenance 7 1 Safety instructions on disinfection and cleaning A 52 When disinfecting the instrument make sure to observe the following safety instructions e Disinfection and Cleaning Chapter 2 3 10 e Handling microtome knives blades Chapter 2 3 4 e Defrosting handling frozen tissue Chapter 2 3 7 e Frozen instrument parts and accessories Chapter 2 3 8 e Infectious radioactive material Chapter 2 3 9 7 2 Spray disinfection with Leica Cryofect For practical spray disinfection we recommend Leica Cryofect which is fully effective at tem peratures in the negative range down to 20 C e Set chamber temperature 20 C or warmer e Remove specimens from the chamber and store safely e Carefully remove knife blade from knife holder e Remove section waste tray from cryocham ber and empty e Remove re
26. Readjust clearance angle set ting Condensation forms on anti roll plate and knife during cleaning 48 Brush forceps cloth or other cleaning item too warm Accessories and cleaning material to be used pre cooled only Store on storage shelves inside the cryochamber Instruction Manual V1 1 02 2000 6 Troubleshooting applications tips Problem Causes Remedies Anti roll plate damaged after adjustment Anti roll plate protrudes too far beyond the knife edge Adjust ment was done in the direction of the knife Replace anti roll plate in future lift away from the knife when aligning Handle anti roll plate more carefully Thick thin sections Wrong temperature for the tissue to be sectioned Knife profile inappropriate for the specimen to be sectioned Ice build up on the knife back Handwheel rotation not uni form or inappropriate rotation speed Knife not clamped tightly enough Specimen disc not clamped correctly Embedding medium poured onto cold specimen disc spec imen comes off the disc once frozen Blunt knife Incorrect clearance angle Specimen dried out Select correct temperature Wait until correct temperature has been reached Use knife with different profile c or d or possible switch to disposable blade system Remove ice Adapt speed Check knife clamping Check disc clamping Place embedding medium on luke warm spec
27. SIE T T E m p olt 3 5 c PEE TET ET ey al e Press CH SJE T T E M P 0 T 3 5 C RRR Specimen cooling is switched back on Despite the possibility of switching the specimen cooling system back on immediately after the automatic chamber defrost cycle we recom mend the automatic defrost cycle be set at a point in time when the entire procedure can be controlled automatically See also information on menu items 6 and 7 in chapter 5 3 4 Menu functions il Instruction Manual V1 1 02 2000 5 Operation 5 7 2 Manual chamber defrost cycle In addition to the automatic defrost cycle a manual chamber defrost cycle can be activat ed when necessary e Press until menu item Set chamber temperature is displayed SEIT TJE M P c T 3 0 C ASSISI I 8 e Press S amp A brief sound signals that the manual chamber defrost cycle has started is elt t elmjp elT 3 o c terna yk IN a The manual defrost cycle ends either when the selected defrost cycle duration is over or when a chamber temperature of 5 C is reached To abort the manual defrost cycle e Go back to menu item Set chamber tem perature and press again Instruments with specimen cooling When defrosting starts to avoid icing the spec imen head is automatically adjusted to a tem perature between 10 C and 5 C i e the set temperature value is deactivated temp
28. ace for quick specimen freezing Freezing specimens with the 90 prism e Apply sufficient amount of embedding medi um onto ambient temperature specimen disc add specimen and orient e Insert specimen disc with specimen into the receptacle 4 of the prism e Press to activate menu item Fast Object Freezing and activate function by pressing see chapter 5 3 4 Menu functions item 4 40 The specimen head Is now automatically cooled down to the lowest temperature 50 C e Once the specimen is frozen remove the disc from the prism and store it in the quick freeze shelf until inserting it into the speci men head e Press again to activate menu item Fast Object Freezing and deactivate the function by pressing once again see chapter 5 3 4 Menu functions item 4 The specimen head temperature now automati cally returns to the previously selected set val ue e Loosen screw 3 at specimen head and re move prism 1 e Store prism inside the cryochamber 5 5 3 Optional accessories for freezing specimens onto specimen discs and for removing frozen specimens Mobile heat extractor In addition to the stationary heat extractor part of standard delivery a mobile heat extractor is avallable as an optional accessory see Ap pendix Ordering information accessories e Store heat extractor in chamber to precool e Place onto specimen in quick freeze shelf e Remove as soon as speci
29. amber Temperature selection range Defrosting Refrigeration capacity Safety factor Refrigerant Compressor oil Quick freeze shelf Maximum temperature Number of quick freeze stations Specimen cooling Option Temperature range Defrosting Refrigeration capacity Safety factor Refrigerant Compressor oil 22 CM3050S 10 100 V AC 10 50 Hz 1800 VA 35 A eff T15A M3 2 Il 1800 J s 50 Hz 0 C to 40 C 2K 0K CM3050S 1 230 V AC 10 50 Hz 1800 VA 25 A eff T10A T1 2 Il 1800 J s CM3050S 8 CM3050S 9 240 V AC 10 100V AC 10 50 Hz 1800 VA 25 A eff T10A T1 2 Il 1800 J s Automatic hot gas defrost cycle programmable in 15 min increments 1 automatic defrost cycle per 24 hours Duration 6 12 minutes Manual defrost cycle 690 W 3 280 g 5g refrigerant R 404A 0 6 EMKARATE RL 22S ICI 43 C 0 K 2K 10 10 C to 50 C 3K Manual defrost cycle electric 320 W 3 185 g 5g refrigerant R 404A 0 41 alpha 22 Kyodo or RENISO E22 Fuchs 60 Hz 1800 VA 30 A eff T15A M3 2 Il 1800 J s 60 Hz 0 C to 40 C 2 K 0K Automatic hot gas defrost cycle programmable in 15 min increments 1 automatic defrost cycle per 24 hours Duration 6 12 minutes Manual defrost cycle 690 W 3 280 g 5g refrigerant R 404A 0 6 EMKARATE RL 22S ICI 43 C 0 K 2K 10 10 C to 50 C 3K Man
30. ant Place an adequate container underneath the drain tube at the right side of the instrument Pull stopper out of chamber bottom and drain off disinfecting solution into the con tainer Put stopper back into drain in cryochamber Leica CM 3050 S Cryostat Disinfection cleaning and maintenance e Allow cryochamber to dry thoroughly e Reinstall microtome see chapter 7 5 3 e Only when they are completely dry reinsert the disinfected accessories tools into the cryochamber e Reinsert the sliding window e Switch the instrument back on e Once a suitable chamber temperature has been reached return the specimens to the cryochamber 7 4 Cleaning e When necessary the instrument surfaces can be cleaned with mild commercial house hold cleaners e Surfaces which are potentially contaminated infectious germs should always be cleaned with alcohol disinfectants 53 7 Disinfection cleaning and maintenance 7 5 Removing reinstalling the microtome 7 5 1 Removing the microtome make sure to observe the following A When _removing the microtome safety instructions e Removing reinstalling the microtome Chapter 2 3 11 e Defrosting handling frozen tissue Chapter 2 3 7 e Infectious radioactive material Chapter 2 3 9 e Disinfection and cleaning Chapter 2 3 10 e Prior to removing the microtome carry out preparation steps as per chapter 7 3 s
31. cessories 1 Handwheel including screw spring washer and cover disc Heat extractor stationary Low temperature stabilizer for heat extractor Specimen discs 30 mm Storage shelf right Storage shelf left Section waste tray Rubber mat Cover for quick freeze shelf Brushes brush fine and Leica brush Shelf for brushes Tool set 1 Allen key size 4 1 Allen key size 5 1 Allen key size 6 Single ended open jaw wrench 1 Bottle of embedding medium for cryosectioning 125 ml 1 Bottle of cryostat oil No 407 100 ml 1 Instruction manual German English French Spanish aa N 2 SD PI m Gd In addition to the above Instruments with specimen cooling 1 90 Prism for low temperature quick freezing Instruments with sectioning motor 1 Footswitch with protective guard Configured instruments 1 Knife holder base 1 Knife holder with accessories Leica CM 3050 S Cryostat Further accessories Further accessories which you ordered will be included in the box containing the standard de livery Items il Knife holders are delivered with anti roll guide knife guard and a separate instruction manual In case of non configured instru ments the knife holder is not a part of standard delivery but must be ordered separately e Check all delivered parts against the pack ing list and against your order to verify whether the delivery is complete If there is any diffe
32. cording to its designated use and according to the instructions given in this manual Damage resulting from improper use of the product and or faulty operation invalidates the warranty Leica will not assume liability for any such damage 8 2 Technical service information lf you require technical service or need replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information Model name and serial number of the instrument Location of the instrument and name of the person to contact Reason for the service call Delivery date of the instrument If not accompanied by an official return authorization form Leica Microsys tems Nussloch GmbH does not accept any returned merchandise Leica CM3050 S Cryostat 57 Warranty and service 8 2 1 Important information for repairs returns of cryostats If there Is any risk that the instrument or parts of it are contaminated with in fectious or radioactive substances the customer must disinfect decontami nate the Instrument prior to returning it or to having it repaired on site The customer must expressly confirm disinfection decontamination indicat ing as well the disinfection decontamination method used a On site repairs Leica Technical Service personnel is instructed to ask for proof of disin fection decontamination and the method s that has have been used b Returned instr
33. e i e the value from which the preset counter counts downward can be selected in the corresponding menu item In order to be able to set a reference value the preset counter function must be active If the preset counter is deactivated see diagrams above press Q to activate Leica CM 3050 S Cryostat Now there are two options 1 Increase or decrease the actual reference value by pressing J or 4 2 Press 4 and simultaneously in order to reload the former reference value 0 when setting up the instrument and then press ei ther or individually in order to select the desired reference value Depending on where you start from and where you want to go method 1 or method 2 is quicker see ex amples Example 1 Actual preset counter reading 50 Former reference value 300 New reference value of 65 to be selected Method 1 is most convenient e Increase from 50 to 65 by pressing 4 Example 2 Actual preset counter reading 50 Former reference value 300 New reference value of 320 to be selected Method 2 is most convenient e Press and simultaneously to reload old reference value 300 e Increase from 300 to 320 by pressing 4 For further Information on the preset counter please refer to chapter 5 3 6 Item 4 Preset counter 29 5 Operation 4 Quick freezing at maximum specimen temperature instruments with specimen cooling only Press key until
34. eed position handwheel locked Others can be displayed by pressing the arrow keys e g selected section thickness preset counter status etc See chapter 5 3 5 Status indication and chap ter 5 3 6 Displaying state of refrigeration sec tion thickness and counters Instruction Manual V1 1 02 2000 5 Operation 5 3 3 Display indications when switching on Meaning of indication Operator activity Initialization phase 1 Initialization phase 2 Software version Selected display language Select language via keys ne Lv Sectioning motor speed level slow fast Select speed level via keys Temperature prior to 4 SETT I 7 gt n Temperature upon switching on gt 0 C gt no pe or ele WEIR FIA LJ UL RIE Acknowledge indication by pressing once briefly cit te jojr ec CIT A d 4c TOIT 1 C DIR Mit c R 10 T 0 M E FDS SSR eee lf this indication is displayed Do not switch on i n i the instrument instrument To unlock press until the display back Prior to switching on both microtome and ac ground lights up cessories must be completely dry Remove microtome accessories disinfect if applicable then dry thoroughly and reinstall CT 96 Olt ee gt See chapters 7 1 7 5 m M HIOIMIE pepe Indication in instruments with specimen cooling Instruments without specimen cooling
35. ements Make sure to read and follow all safety instructions in chapter 2 3 2 Site requirements 3 1 1 General site requirements e No direct sunlight e Electrical power supply within distance length of power cord approx 4 meters do not use extension cords No draft caused by air conditioning etc Even floor surface Practically vibration free floor Handwheel easily accessible Room temperature constantly below 22 C Relative humidity of air maximum 60 High ambient temperature and or high air humidity negatively affect instrument cooling performance il 3 1 2 Electrical connections Make sure to read and follow all safety instructions in chapter 2 3 3 Electrical connections 16 3 2 Unpacking and installation Unpacking instructions are always located in a transparent protective envelope on the outside of the instrument shipping crate Make sure to read and follow all safety instructions provided in chapter 2 3 1 Transport and on the unpacking instructions 3 2 1 Repacking We recommend to keep the original shipping crate and the unpacking Instructions for the Leica CM3050 S For repacking proceed as per unpacking in structions in reverse order Instruction Manual V1 1 02 2000 3 Installation 3 3 Standard delivery Together with the Leica CM3050 S instrument you receive a box containing the following stan dard ac
36. er instruments or persons Damage resulting from improper use of the product and or faulty operation invalidates the warranty Leica will not assume liability for any such dam age 1 2 Standard delivery Electrohydraulic height adjustment device for cryostat series CM3050 S complete outfit con sisting of a U shaped frame on four height ad justable feet and a two key control unit The height adjustment device is available in 2 voltages 1 230 240 V 50 Hz order no 0443 26147 2 230 240 V 50 Hz order no 0443 26147 Leica CM 3050 S Cryostat 1 3 Safety instructions Prior to operating the height adjustment device make sure to read the present instruction manu al Pay special attention to the following safety instructions A Prior to connecting the height ad justment device to mains check to make sure that the nominal voltage and frequency indications on the device name plate correspond to the actual mains voltage in your lab Do not move the height adjustment device with the Leica CM3050 S in stalled on the frame If the place of installation needs to be changed first take the Leica CM3050 S down from the height adjustment device and then move both devices sepa rately Never place your feet underneath the U frame when lowering the height adjustment device Risk of injury Only authorized Leica technical ser vice engineers may carry out re pairs on both the Leica CM3050 S
37. harp cutting edges and can cause serious Injuries Therefore e Handle knives blades with utmost care e Never leave any knives blades in unpro tected places e Never place a knife no matter where with the cutting edge facing upwards e Never try to catch a falling knife e Always insert the specimen before inserting the knife 2 3 5 Knife guard handwheel lock e Always cover the cutting edge with the knife guard and lock the handwheel e Prior to doing any work on knife and or specimen e Prior to exchanging specimens e During work breaks 2 3 6 Motorized sectioning e Do not interrupt sectioning trimming by set ting the sliding potentiometer to zero speed gt This does not really switch the sectioning function off it only operates at 0 speed If the sliding potentiometer is accidental ly moved the instrument will resume sec tioning immediately risk of injury e During motorized sectioning always center the handwheel grip Leica CM3050 S Cryostat 2 3 7 Defrosting Handling frozen tissue e Never leave specimens unattended in the cryochamber over an extended period of time gt In case of power failure or instrument failure or during the automatic defrost cy cle tissue material can be destroyed e During the defrost cycle the cryochamber Is partially warmed Therefore e Remove sensitive specimens from the chamber prior to defrosting e lf automatic defrosting is program
38. hnical Service in the Remedy column please do so Never try to solve these problems yourself Instruction Manual V1 1 02 2000 5 Operation 5 2 Switching on fuses 2 Automatic cutout for sectioning motor 1 Combination Mains switch automatic cutout for the instrument Switching on e To switch on the instrument place switch 1 in upper lock in position Mains switch automatic cutout for the instrument In addition to its function as ON OFF switch switch 1 is also the automatic cutout for the instrument If the automatic cutout has been triggered e g caused by overcurrent e Put switch 1 back in upper lock in position Leica CM 3050 S Cryostat Automatic cutout for sectioning motor The instrument is equipped with an automatic cutout 2 for the sectioning motor If the sectioning motor is overloaded e g when sectioning extremely hard specimens etc the automatic cutout 2 will be triggered To make the instrument functional again after the automatic cutout 2 has been triggered e Push automatic cutout 2 inwards until it locks into position 25 5 Operation 5 3 Control panel 1 4 LEICA 30508 5 3 1 Key functions in control panel 1 ON OFF key for cryochamber illumination N To select the menu items for setting changing instrument parameters In the status indication pressing the ar A row keys displays the individual current pa
39. ily operation of the instrument 39 Daily routine steps when finishing work 43 Defrost key 26 Defrosting 13 44 45 Defrosting Handling frozen tissue 13 Defrosting the specimen head 45 Designated use 8 Disassembling the specimen head 54 Disinfection and cleaning 14 52 53 Display message Dry microtome 15 Displayed error messages 46 Displaying parameters with arrow keys 32 Dry microtome 15 27 Duration of defrost cycle setting 30 64 E EC Declaration of Conformity 59 Electrical connections 12 16 19 Emergency stop function 9 11 36 Exchanging the fluorscent light lamp 15 56 F Finishing work 43 Fluorescent light lamp 15 56 Foot switch use for emergency stop 11 Footswitch 11 19 20 34 36 46 50 Footswitch port 20 Freezing specimens onto specimen discs 39 40 Frozen instrument parts accessories 13 Frozen specimens 45 Fuses 25 G Glass anti roll guide 60 H Handling microtome knives blades 13 Handwheel grip centering 9 10 Handwheel grip centering motorized sectioning 13 Handwheel lock 9 10 13 31 Infectious radioactive material 14 Inserting specimen discs into the specimen head 41 Installing inserting accessories 18 Installing the footswitch 19 Installing the handwheel 18 Instrument nameplate 3 7 Integrated safety devices 9 10 11 Intermittent stroke 34 K Knife guard 9 11 13 60 Knife guard handwheel lock 13 Knife holder CN 20 Knife holder CS 20 Knife h
40. imen disc inside the cryochamber place the specimen onto the disc and freeze in quick freeze shelf Use different part of knife or replace knife Set correct clearance angle Prepare new specimen Tissue sticks to the anti roll plate Anti roll plate too warm or incorrectly adjusted Static electricity Fat on corner or edge of anti roll plate Knife rusty Cool or readjust anti roll plate Eliminate cause Remove fat alcohol Remove rust Flattened sections curl up as soon as anti roll plate is lifted from the knife Leica CM3050 S Cryostat Static electricity or draft Anti roll plate too warm Eliminate cause Coolanti roll plate 49 6 Troubleshooting applications tips Problem Causes Remedies Sections tear Temperature too low for the tissue to be sectioned Knife blunt dirty dusty frosted or rusty Upper edge of anti roll plate damaged Hard particles in the tissue Rear face of knife dirty Select higher temperature and wait for the new temperature to be reached Eliminate cause gt see details on individual problems Exchange anti roll plate If application permits section at lower plane Clean Inconsistent or insufficient specimen feed Microtome was not completely dry when refrigeration was switched on ice build up inside the micrometer mechanism Microtome defective Remove microtome from cham ber and dr
41. ing frozen speci mens Make sure to remove sensitive speci mens from the cryochamber before the defrost cycle starts 5 7 1 Automatic chamber defrost cycle The automatic defrost cycle is carried out once every 24 hours at the preselected time For details see chapter 5 3 4 Menu functions items 6 and 7 During the defrost cycle the corresponding as terisk is displayed inversely CiT 3 o e c olt s 5 c mjajHjojM Ej J E Once the defrost cycle is finished the instrument automatically switches back to refrigeration Instruments with specimen cooling When defrosting starts to avoid icing the speci men head is automatically adjusted to a temper ature between 10 C and 5 C i e the set tem perature value is deactivated temporarily 44 The specimen head refrigeration is automatical ly switched back on when e at least 4 hours have passed since the de frost cycle was completed e and the actual chamber temperature devi ates less than 5 K from set value After the specimen head refrigeration has been switched back on the specimen head tempera ture rises briefly to 10 C and then decreases until it reaches the set value Switching specimen cooling back on immedi ately after defrosting As soon as the chamber defrost cycle is com pleted specimen cooling can be switched back on immediately e Press until menu item Set specimen temperature is displayed
42. ior writ ten permission of Leica Microsystems Nussloch GmbH The serial number and the year of manufacture are specified on the nameplate at the back of the instrument Leica Microsystems Nussloch GmbH Table of Contents 1 Important iniorniatioh a della 7 Le Sympos USEC CIS aU al QING acc a 7 Ped BE 055 0 af 2 US ee Pn oe Re ee ere 8 k QU ANME AMON OL personne ssccticictic ces cinessidentitesdnsasieaiecpeastatea wicvoaibataatouude alata taatataeanietenededenioneanevens 8 2l Generaliniormation oninstrument acli 9 De Pdl 9 2 2 integrated Sale WOeviee spaccia 9 2 2 L Lockngtnenanamnee cca 10 2 2 2 Centering the handwheel grip ii 10 2 2 3 EMergenty Sto D MNC UO N crsa R A 11 ZLA RAe GUAT iaioa onare o eno E EaR 11 2 3 Safety instructions handling and operation of the INStTUMENT e 12 Zo Transpor reaa a aa a a 12 2 3 2 Site requirements eee 12 23a Electrical tonne CHON Sneins a Aa 12 2 3 4 Handling microtome knives blades ccccccessssccssssecssssssesessecessesseceesesesseseesessesenceseuseesassesensessnsensas 13 2 935 KNieguardnanadWwhieellotkt a heard 13 23 6 Motorized SOC HONING nile aa 13 293 Delos 13 238 Frozen parts OF theinstrument GING sorgan a a aE 13 2 3 9 Infectous radioactive Material e 14 29 10 Disinfection and CLEANING aaa 14 2 3 11 Removing reinstalling the MICFOTOME iii 15 2 3 12 Display Message Dry microtome
43. is an important part of the product It must be read carefully before us ing the instrument for the first time and must al ways be kept with the instrument If additional requirements which exceed the scope of this manual are imposed by regula tions and or laws on accident prevention and environmental protection in the country of oper ation appropriate instructions for compliance with such requirements must be added to this manual Read this instruction manual carefully before attempting to use or operate the instrument Please pay particular attention to chapter 2 safety features safety in structions Please read this infor mation even if you are already fa miliar with the operation and use of other Leica products 1 2 Designated use The Leica CM3050 S is a powerful cryostat for routine as well as research applications in biol ogy medicine and industry The instrument has been designed for rapid freezing and sectioning of tissue samples The instrument has not been designed for un attended storage of tissue material The instrument may only be operated within the scope of its designated use as described above and as per the Instructions given in this manual Any other use of the instrument is considered improper 1 3 Qualification of personnel The Leica CM3050 S may only be operated by trained laboratory personnel All laboratory personnel designated to operate the instrument must ca
44. magnetic Compatibility including their amendments up to the date mentioned below The following harmonized standards were applied DIN EN 292 DIN EN 61010 1 EN 50082 1 EN 55011 IEC 1000 4 The following national standards guidelines and specifications were applied MedGV In addition the following in house standards were applied DIN EN 9001 A complete set of technical documentation is available An instruction manual for subject product is available Leica Microsystems Nussloch GmbH Postfach 1120 D 69222 Nussloch Germany July 21 1999 Managing Director Leica CM3050 S Cryostat 63 Alphabetical index A Accessories 17 40 41 60 Anti roll guide glass 60 Automatic chamber defrost cycle 44 Automatic cutout for sectioning motor 20 Auxiliary clamping device for knife holder CS 60 Available models of the CM3050 S 17 B Brush shelf 20 C CE Mark declaration of conformity 59 Chamber defrost cycle automatic 44 Chamber defrost cycle manual 45 Cleaning 53 CM3050 S overview 20 Coarse feed functions 37 Connecting the footswitch 19 Consumables 60 Continuous stroke 34 Control panel 1 20 26 27 28 29 30 31 32 Control panel 2 20 33 34 35 36 37 38 cryochamber illumination ON OFF key 26 Cryochamber temperature 29 cryohamber illumination exchange 56 cryohamber illumination safety instructions 15 Cryostat CM3050 S overview 20 Cryostat oil No 407 60 D Da
45. maining section waste from cryo chamber e Working accessories brushes specimen holders etc can be disinfected inside the cryochamber e Once the selected chamber temperature has been reached spray Leica Cryofect on the surfaces and accessories to be disinfected e Allow to soak for 15 minutes e Use paper towels or another type of disposable cleaning cloths to wipe up disinfectant and remaining section waste e Dispose of paper towels together with sec tion waste e f previously altered set chamber tempera ture back to Its former value e Putspecimens back into the cryochamber Instruction Manual V1 1 02 2000 7 7 3 Disinfection with conventional disinfectants Switch the instrument off Carefully remove knife blade as well as spec imens and accessories from the chamber Remove section waste from the chamber and dispose of It Remove sliding window see chapter 7 6 Ex changing the fluorescent light lamp Remove microtome see chapter 7 5 1 Removing the microtome Allow the microtome to thaw disinfect and allow to dry thoroughly Hi Drying the microtome e Use a hairdryer dry and before reinstalling into the chamber allow the microtome to cool down to room temperature e Allow to dry at room temperature at least over night longer if necessary Disinfect and dry disassembled parts accessories and tools Disinfect chamber walls with commercial alcohol disinfect
46. med to take place during the night remember to remove all specimens from the cryo chamber prior to leaving work 2 3 8 Frozen parts of the instrument and frozen accessories e Prolonged contact of bare skin to frozen surfaces of the instrument or to frozen accessories Specimen discs knife holder shelves etc can cause frostbite e Wear protective gloves 13 2 Safety A 2 3 9 Infectious radioactive material 14 Danger Use caution when working with potentially infectious specimens gt Risk of infection When working with potentially infectious radioactive specimens e Wear protective clothes gloves protec tive boots mask lab coat in compliance with radiation safety regulations and or in house regulations on handling infec tious radioactive material When working with radioactive specimens e Comply with applicable radiation safety regulations e Dispose of radioactive specimen waste ac cording to applicable regulations 2 3 10 Disinfection and cleaning Prior to disinfection switch the instrument off and unplug It from mains For removal of the microtome from the cryo chamber see chapter 2 3 11 Removing the microtome For disinfection wear protective gear gloves mask lab coat etc For disinfection only use alcohol based dis infectants Do not use solvents xylene acetone etc for cleaning or disinfection Do not spray disinfectants into the evapora t
47. men Is frozen Instruction Manual V1 1 02 2000 5 Operation Thermoblock 5 5 4 Inserting specimen discs into the specimen head The thermoblock makes it easy to remove fro zen specimens from the specimen discs e Lock handwheel in upper position Do not store the thermoblock inside If a knife has already been inserted the cryochamber store at room into the knife holder cover knife temperature and only place into the edge with knife guard chamber for removing specimens from discs e Place cap 9 on one of the two ends so that the correct size opening 7 for the disc to be e Loosen clamping screw 3 inserted is exposed e Insert specimen disc in opening 2 rear e Insert pin 6 of specimen disc 5 into the surface of specimen disc must fit tightly opening 7 against surface of specimen head e After about 20 seconds remove the frozen Tighten clamping screw 3 specimen from the specimen disc use tweezers e If cap 9 fits too loosely readjust with 555 Specimen orientation screw 10 Do not tighten screw too much e Once the specimen has been detached from Open clamping lever 4 gt 4a the disc remove the thermoblock from the e Orient specimen via orienting screws 1 cryochamber and 5 e Retighten clamping lever 4 Leica CM 3050 S Cryostat 41 5 Operation 5 5 6 Trimming sectioning Inserting the knife e Insert the knife disposable blade i
48. nains 33 SAT SCICCUNG aS CCHONING ModE ira ea 34 5A 2 oeng AME SOCHONING WINGO W annn aa AAT A 35 543 Selecting sectioning speeder 36 AA EMMONS IVE VS OD aen a eta 36 BA BUG Ocak S CEE Or K OY liacle 37 5 4 6 Trimming and sectioning FUNCTIONS ii 38 BAT Setting nesecuontniekmessonsilae er 38 55 Dally Operation OF IME Instrument cicirine aA R AN 39 09l Freezingispecimens ento speciMeNiisa allora 39 5 5 2 Freezing SPECIMENS ONTO SPECIMEN cccceeccesssessessssscessncecsessesessnsecseseusesensassesansesenseusaseesarsnseasensaneas 40 553 Opto nal aCCCSSONIES OF IFOOZING kirosin E A 40 5 5 4 Inserting specimen discs into the seats ecuscaitenasassesasnvasiaavede a 41 Leica CM3050 S Cryostat 5 Table of contents 2 95 S EcIMENoFrend to laicale 41 5 9 0 LEMMNG SeCUONNI amici 42 n 6 LEIMNISIINOMWOri nica 43 5 6 Daily routine Ste pS narin a a 43 5 6 2 Switching off the instrument for a 43 S I 8516 511110 EPA EER REA VARESE a TARGET 44 Sal AUTOMatte GhambperFdeftrostoy0 esca 44 5l 2 Manual enambperdetrostovoieaclccceicaiaaa 45 5 7 3 Defrosting the specimen head 45 6 Troubleshooting applications tips iiiiiiina 46 Bale Displayed eror message santa 46 bel OTHER ERROR IMGIG GU OMS sanman a a A a R Aa 46 6 2 Potential problems causes and remedies sssssssssssssrssunurunurunsrunnannninarnnururnnununnnununnnnnnnnnnnnnnnnnnnnrnnnnnns 47 6 3 Reference chart for temperature settings IN
49. nd the desired set temperature can be selected with one of the arrow keys Press arrow key and release upon reaching the desired value CIT 4c olt 3 5 c mjajHjojM E I I 1 Note on the arrow key function Each time one of the arrow keys is pressed briefly the value displayed in the menu will be changed one step up or down lf an arrow key is pressed and held the value will change continually auto repeat function When holding the key scrolling speed will in crease after a while As soon as a value is displayed it is atthe same time automatically stored Instruction Manual V1 1 02 2000 5 Operation 2 Cryochamber temperature ON Press key until menu item Set chamber temperature is displayed gt Continue as described for specimen temperature 3 Preset counter instruments with sectioning motor only Press key until menu item Preset Counter is displayed _ PIRJE S E T C O U N T E R Preset counter activated _ PIRJE SJE T C o U N T E R PE TT tT TT tt Jafno 5 Preset counter deactivated Preset counter function The preset counter allows the user to carry out a predefined number of sections in the Continu ous stroke sectioning mode Once the preselected number of sections has been carried out section counter reading 0 the specimen head will stop automatically in the lower limit position The reference valu
50. nife into chamber to precool Place all tools needed for section prepara tion into the chamber Close the sliding window For a complete overview of all indi vidual parts gt see Chapter 4 1 Overview Instruction Manual V1 1 02 2000 3 4 3 The footswitch Function The footswitch performs the same task as the RUN STOP and RUN ENABLE keys activating deactivating motorized sectioning trimming In addition the footswitch can be used to acti vate the emergency stop function Models with footswitch All instruments with sectioning motor Important note In all instrument models that are delivered with footswitch the foot switch must be installed Other wise the instruments are not func tional Leica CM 3050 S Cryostat 3 Installation Connecting the footswitch e Insert footswitch into port 1 and secure 3 5 Prior to switching on the instrument A A After transport observe a waiting pe riod of at least 4 hours before turning the instrument on See also safety instructions in chapter 2 3 1 Trans port Have you observed all safety in structions in chapters 2 3 2 Site re quirements and 2 3 3 Electrical connections If not gt Please read chapters 2 3 2 and 2 3 3 e Insert mains plug into wall outlet e Continue with chapter 5 1 Setting up the in strument 19 4 Instrument Properties
51. nto the knife holder e Select appropriate clearance angle at knife holder settings between 4 and 6 are suit able for the majority of applications For details on how to insert and ad just the knives please refer to the separate instruction manual s for your knife holder s See also chapter 2 2 Integrated safety devices incl subchapter 2 2 4 Knife guard il Manual trimming e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Unlock handwheel e Use motorized coarse feed to bring speci men close to knife see chapter 5 4 5 Coarse feed functions e Select desired section thickness see chap ter 5 4 7 Setting the section thickness e Move anti roll plate away from knife e Rotate handwheel to trim specimen down to desired sectioning plane Motorized trimming e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Unlock handwheel e Center handwheel grip See chapter 2 2 2 42 e Use motorized coarse feed to bring speci men close to knife see chapter 5 4 5 Coarse feed functions e Move anti roll plate away from knife gt Next steps see chapter 5 4 6 Trimming Sectioning e Adjust knife knife holder relative to speci men see Knife holder instruction manual e Place anti roll guide onto knife e Orient specimen if necessary see chapter 5 5
52. o be pressed while the continuous sectioning mode is in motion in this case the preset counter Is set to 0 and sectioning stops upon reaching the lower limit position After it has been set to 0 the preset counter Is Inactive Reloading the former reference value Press and y simultaneously Reloading the former reference value Is possible also while the continuous sectioning mode is In motion Upon pressing both arrow keys the instrument continues sectioning without interruption start ing to count down from the old reference value Instruction Manual V1 1 02 2000 5 Operation 5 4 Control panel 2 Instruments without sectioning motor Instruments with sectioning motor In instruments without sec tioning motor control panel 2 only consists of functional area B see below In instruments with section ing motor the control panel is equipped with three func tional areas A B and C see large diagram A Functional area A e Sectioning modes e Sectioning window e Sectioning speed e Emergency stop function MTT 5 10 30 50 100 150 B TRIM Functional area B Functional area B e Coarse feed e Coarse feed e Trimming e Trimming e Section thickness selection e Section thickness selection ES B RUN RUN STOP ENABLE STOP Functional area C e Start Stop sectioning Leica CM 3050 S Cryostat 33 5 Operation Functional areas A and C Instruments with
53. of cryostats important info 58 Rubber mat 20 S Safety instructions handling and operation of the 13 14 15 Section thickness indication 32 Leica CM 3050 S Cryostat Section thickness setting 38 Section thickness range 38 Section waste tray 20 Sectioning 42 Sectioning modes single continuous intermittent 34 Sectioning speed selection 36 Sectioning window 35 Selecting a sectioning mode 34 Serial number 3 7 Shutdown and disposal of the instrument 58 Single stroke 34 Site requirements 12 16 Specimen discs 20 41 60 Specimen head w o specimen cooling 20 Specimen head with specimen cooling option 20 Specimen orientation 41 Standard delivery 17 Start stop motorized sectioning 34 Start of automatic defrost cycle setting 30 Stationary heat extractor 5 20 39 Status indication 31 32 Storage shelf left 20 Storage shelf right 20 Switching between trimming and sectioning 38 Switching off the instrument for longer 43 Switching on the instrument 25 Symbols in this manual and their meaning 7 I Technical Data 22 23 Technical service information 57 Temperature settings reference chart 51 Thermoblock 20 41 60 Time parameters setting 28 30 Transport 12 Trimming 42 Trimming sectioning 42 Trimming thickness 38 Troubleshooting 46 47 48 49 50 U Unpacking and installation 16 V Vmax key 36 W Warranty 57 65 Notes 66 Instruction Manual V1 1 02 2000
54. of special category waste e fnecessary carry out a complete disinfection procedure see chapter 7 Disinfection cleaning and maintenance e Remove all specimens from the chamber and store safely e Close sliding window e Switch off cryochamber illumination e Use lock unlock key to lock display in control panel 1 Display background dark locked CITH SAAC OTS eC BE SOG dc GE Do not switch off mains switch automatic cutout see chapter 5 2 Switching on fuses Leica CM 3050 S Cryostat 5 6 2 Switching off the instrument for a longer period of time If you do not plan to use the Instrument for an extended period of time it can be switched off However since this involves a lot of work see below it should be considered carefully whether switching off the instrument is really the better option If the instrument is switched off the microtome must be removed Then the microtome cryo chamber and all accessories must be disinfect ed and dried e For that purpose follow the instructions in chapters 7 1 through 7 5 3 43 5 Operation 5 7 Defrosting The Leica CM3050 S has 3 instruments without specimen cooling 2 different defrosting options Automatic chamber defrost cycle Manual chamber defrost cycle Specimen head defrosting manual instru ments with specimen cooling only Read the safety instructions in chap ter 2 3 7 Defrost
55. older base for knife holders CN CE and CS 20 60 Knife holder CE 20 Knife holders CN CE CS 60 Knife rest with section collecting tray for knife 60 Knife support bar for knife holder CS 60 Knives blades Sehe Handling microtome knives blades Instruction Manual V1 1 02 2000 Alphabetical Index L Leica CM3050 S available models 17 Leica Cryofect for spray disinfection 52 Lock unlock key 26 Mains switch automatic cutout for instrument 20 Maintenance 15 56 Manual chamber defrost cycle 45 Maximum sectioning speed Vmax 36 Menu functions 26 28 Menu key 26 Mobile heat extractor 20 40 60 Motorized sectioning 13 34 36 Motorized sectioning start stop 34 Motorized trimming 38 0 OCT compound for freezing tissu 60 Operating the instrument for the first time 24 Overview of the instrument 20 P Potential problems causes and remedies 47 48 49 50 Preset counter indicating parameters 32 Preset counter setting parameters 29 Prism 90 20 40 60 Q Qualification of personnel 8 Quick freeze shelf 20 39 Quick freezing at maximum specimen temperature 30 40 Quick freezing specimens 39 40 R Reference chart for temperature settings 51 Refrigeration parameters indication 32 Refrigeration parameters setting 28 29 30 Removing reinstalling the microtome 15 54 55 Removing frozen specimens from specimen discs 40 Removing the sliding window 56 Repacking 16 Repairs returns
56. or gt Risk of icing Explosion hazard when working with alcohol Make sure the premises are appropriately ventilated When using disinfectants and detergents comply with all safety instructions supplied by the manufacturer of the product Dispose of waste liquids from disinfection cleaning as well as of sectioning waste ac cording to applicable regulations on disposal of special category waste Disinfected accessories must be thoroughly dry when reinserting them into the chamber gt Risk of icing Make sure the chamber is completely dry before switching the instrument back on gt Explosion hazard through alcohol vapors gt Risk of icing Instruction Manual V1 1 02 2000 A 2 Safety 2 3 11 Removing reinstalling the microtome e Before removing the microtome Switch instrument off Unplug from mains Place handwheel grip in lowest position and lock gt When removing the microtome the Specimen head must always be lok ked in the lowest position Otherwise the upper part of the slot cover might be bent and consequently damaged e When removing the microtome Wear gloves when removing the micro tome while it is still frozen gt Risk of frost bite On instruments with specimen cooling do not distort the refrigerating tube If distorted it might break causing ex tremely cold refrigerantto escape gt Riskoffrost bite e Before reinstalling the microtome Microtome mu
57. orarily same as with automatic defrost cycle see chapter 5 7 1 Automatic chamber defrost cy cle 10 seconds after the manual chamber defrost cy cle has been completed the specimen head re frigeration is switched back on cooling down to the set value Leica CM 3050 S Cryostat 5 7 3 Defrosting the specimen head instruments with specimen cooling only To activate specimen head defrosting e Press until menu item Set specimen temperature Is displayed S EIT TJE MIP o T 3 5 c PET TET EE TT T Gel e Press 4 A brief sound signals that the manual chamber defrost cycle has started S E T TEMP OT 3 5 c ot the TR tached Specimen head temperature increases to be tween 20 C and 30 C for a duration of 10 minutes after which it returns to the set value To abort specimen head defrosting e Press until menu item Set specimen temperature is displayed and press once more 45 6 Troubleshooting applications tips 6 1 Displayed error messages The autodiagnosis function of the CM3050 S displays error messages In case of certain mal functions 1 Power failure This error message Is displayed after the instru ment had been disconnected from mains either due to power failure or because the mains switch had been turned off C T 3 0 c o T 3 5 c _J_ PJoMjE R FIAT IL U RIE e To acknowledge the message press ay for about
58. ork with the instrument is resumed see also chapter 5 7 1 Automatic chamber defrost cycle Defrost cycle duration The duration of the chamber defrost cycle is set to 8 minutes in factory This setting should only be changed if the evaporator is not completely de iced during the 8 minute cycle In such cases increase the defrost cycle duration gradually until the the evaporator is ice free after de frosting The selected defrost cycle duration is valid for both automatic and manual chamber defrost cycle Instruction Manual V1 1 02 2000 5 Operation 5 3 5 Status display Each individual parameter Is displayed in a certain area of the display screen Upper line In the menu see chapter 5 3 4 indication of selected menu item Standard indication Chamber temperature instruments with specimen cooling chamber and specimen temperature Standard indication upper line Instruments with chamber and specimen cooling CiTI 3 ol ct o T 3 5 c mjajHjojME IIIi I Rel Instruments with chamber cooling only efti falo e e DD mjajHjojM E tT tt te Upper line Lower line Digits 1 and 2 indicate sectioning stroke position e Sectioning stroke outside sectioning window Retraction stroke Sectioning stroke inside sectioning window Sectioning stroke o0 Sectioning window Retraction stroke Leica CM3050 S Cryostat e a E a Lcd
59. plications tips 6 2 Potential problems causes and remedies Problem Causes Remedies Frost on chamber walls and microtome Cryostat is exposed to draft open windows doors air conditioning Sliding window has been open for a long period of time ata very low chamber temperature Frost build up caused by breathing into the cryocham ber Eliminate draft sources or change place of installation of the cryostat Close sliding window Consider wearing a mask Sections smear Specimen not cold enough Knife and or anti roll plate not vet cold enough sections melt Select lower temperature Wait until knife and or anti roll plate have reached chamber temperature Sections splinter Sections not properly flattened Specimen too cold Static electricity draft Specimen not cold enough Large surface specimen Anti roll plate poorly adjusted Anti roll plate poorly aligned relative to knife edge Wrong clearance angle selected Knife blunt or damaged Select higher temperature Eliminate cause Select lower temperature Trim specimen parallel in crease section thickness Readjust anti roll plate Align correctly Set correct clearance angle Use different part of knife or replace knife Sections not properly flattened despite correct temperature and correctly aligned anti roll plate Leica CM3050 S Cryostat Knife and or anti roll plate dirty Edge of anti
60. quirements 12 Do not operate the instrument in rooms with explosion hazard To ensure proper instrument function main tain a minimum distance of 10 cm between walls and or furniture and all sides of the in strument 2 3 3 Electrical connections Do not use extension cords for connecting the instrument to mains gt Fire hazard gt Instrument malfunctions caused by voltage drop During the start up phase of the compressor the nominal voltage must not drop below the values specified in chapter 4 2 Technical data gt The compressor needs a start up current at between 25 and 35 A see chapter 4 2 Technical data Ensure uniform current supply according to specifications gt Electrical power supply deviating from specifications damages the instrument e Therefore arrange for the electrical in Stallations on site to be checked by a trained professional and make sure any necessary upgrades are Installed e Have the circuit protected by a fuse of Its own e Do not connect any other consumers to the same circult e Prior to connecting the instrument to mains make sure the electrical power supply in your laboratory corresponds to the values indicated on the instrument nameplate located at the rear of the in strument Instruction Manual V1 1 02 2000 A i Safety 2 3 4 Handling microtome knives blades e Danger gt Microtome knives and disposable blades have extremely s
61. rameters In the menu pressing the arrow keys changes set values As soon as a value is displayed it is au tomatically stored SY Activates the manual defrost cycle cry ochamber This key is functional only when one of the menu items SET TEMP CT C or SET TEMP OT C last one only applies to instruments with specimen cooling has been selected first Lock unlock key Locks the display to protect against accidental changes of set parameters Activates deactivates a number of functions 5 3 2 Display functions in control panel 1 The display in control panel 1 combines several different functions 1 When switching the instrument on A series of standard indications is displayed during which two basic parameters can be se lected with the arrow keys Language of display indications and speed level of sectioning motor See chapter 5 3 3 Display indications when switching on 2 Menu function activated via menu key Via the menu key a number of parameters can be selected then be activated deactivated via the lock unlock key and or changed via the ar row keys See chapter 5 3 4 Menu functions Setting param eters of refrigeration time and preset counter 3 Status indication function The actual status of selected parameters Is in dicated in the display screen Some of those parameters are displayed auto matically e g sectioning stroke phase coarse f
62. rd and lock the hand wheel All instruments e Prior to doing any work on The message LOCKED in the display of control knife and or specimen panel 1 indicates that the handwheel has been e Prior to exchanging specimens locked e During work breaks Ci T 3 o c JOT 3 5 c The handwheel can be locked in 2 positions rT L o ckkK elb e with the grip in the uppermost position left Unlocking e with the grip in the lowest position right E To unlock push locking pin 2 to the left into position 2a e Display indication LOCKED disappears Instruments with sectioning motor The sectioning motor can now be activated again 2 2 2 Centering the handwheel grip During motorized sectioning for safety reasons always center the handwheel grip e Rotate handwheel until grip 1 isinupperor To center grip 1 pull outwards and pivot lower position a 7 into center of handwheel e To lock press pin 2 to the right into position 2b e When released the grip locks into position gt The upper locking position for pin 2 is marked by a black dot 4 10 Instruction Manual V1 1 02 2000 A i Safety 2 2 3 Emergency stop function instruments with sectioning motor only The emergency stop Is activated via the red emergency stop button in control panel 2 or via the footswitch _ 100 E 50 mmo Control panel 2 Footswi
63. refully read the present instruction manual prior to starting work with the instrument Instruction Manual V1 1 02 2000 A i Safety 2 1 General information on instrument design and safe handling This instrument has been built and tested In ac cordance with the following safety regulations on electrical measuring control regulating and laboratory devices e DINEN292 e DIN EN 61010 1 e EN 50082 1 e EN 55011 e EC 1000 4 as well as according to the international quality standard e DIN ISO 9001 In order to maintain this condition and to ensure safe operation the operator must observe the instructions and warnings contained in this in struction manual Leica CM3050 S Cryostat 2 2 Integrated safety devices The instrument is equipped with the following safety devices e Handwheel lock e Handwheel grip centering e Emergency stop function instruments with sectioning motor only e Knife holder equipped with knife guard The safety devices installed by the manufactu rer of the instrument only constitute the basis of accident prevention Mainly responsible for accident free operation is above all the institution which owns the in strument and in addition the designated per sonnel who operate service or repair the instrument 2 Safety AN 2 2 1 Locking the handwheel Instruments with sectioning motor The sectioning motor is now blocked Always cover the cutting edge with the knife gua
64. rence contact your local Leica office immediately Available models e Basic instrument with sectioning motor without specimen cooling e Basic instrument Without sectioning motor with specimen cooling e Basic instrument with sectioning motor with specimen cooling 17 3 Installation 3 4 Installing inserting accessories 3 4 2 Inserting the accessories 3 4 1 Installing the handwheel Place the rubber mat on top of the housing Insert pin 1 of the handwheel shaft into hole 2 Place spring washer 3 onto screw 4 as shown Tighten screw 4 with an Allen key Cover with selfadhesive disc 5 e To remove the handwheel proceed as de i 18 For purposes of transport e g nar row doors the handwheel can be removed scribed above but in reverse order Insert the storage shelves into the cryo chamber Install the stationary heat extractor into the quick freeze shelf see also drawing in chapter 5 5 1 Freezing specimens onto specimen discs Insert the low temperature stabilizer into the quick freeze shelf it must be located in the pivoting range of the heat extractor see also drawing in chapter 5 5 1 Freezing spec imens onto specimen discs Insert section waste tray and brush shelf Install knife holder base onto microtome base plate and clamp Install knife holder and clamp see knife holder instruction manual for details Place knife case with k
65. st be completely dry gt Humidity in the interior of the micro tome freezes and causes microtome malfunctions and or damage to the microtome All accessories tools removed from the cryochamber must be thoroughly dry be fore putting them back into the chamber gt Risk of icing Leica CM3050 S Cryostat 2 3 12 Display message Dry microtome If the error message Dry Microtome is dis played in control panel 1 the following has happened Cryochamber refrigeration has been in terrupted for an extended period of time e g power failure causing the chamber temperature to rise into the positive digits If this message appears do not switch on the instrument but remove the microtome from the chamber disinfect if necessary and dry thoroughly before reinstalling it into the chamber see chapter 7 1 to 7 5 2 3 13 Maintenance Only technical service engineers authorized by Leica may access the internal compo nents of the instrument for service and re pair The fluorescent light lamp chamber illumi nation unless broken or splintered can be replaced by the user Switch off mains switch Unplug the instrument from mains If the lamp is broken or splintered Have lamp replaced by Leica Technical Service gt Risk of injury Use only those replacement lamps that cor respond to technical specification see chapter 4 2 Technical Data 15 3 Installation 3 1 Site requir
66. tch Activating the emergency stop function e Press emergency stop button or step on footswitch forcefully gt sro STOP red lights up As soon as the emergency stop function is acti vated the sectioning motor stops Deactivating the emergency stop e To deactivate rotate emergency stop button in direction of arrow lf the emergency stop function has been activated by the footswitch unlocking is not necessary func tion is unlocked as soon as the foot switch is released il Leica CM3050 S Cryostat 2 2 4 Knife guard All knife holders are equipped with a knife guard gt see separate instruction manuals on knife holders Always cover the cutting edge with the knife guard e Prior to doing any work on knife and or specimen e Prior to exchanging specimens e During work breaks 11 2 Safety A 2 3 Safety instructions handling and operation of the instrument 2 3 1 Transport To avoid severe damage to the instrument by running it while the compressor oil is dis placed from its regular position e Do nottilt the instrument only transport in an upright position e After transport wait at least 4 hours be fore turning the instrument on gt The compressor oil may have been displaced during transport and must settle to its original position be fore switchingthe instrument on Otherwise the instrument may be severely damaged 2 3 2 Site re
67. tioning mode 3 Single stroke e Simultaneously press and release RUN STOP and RUN ENABLE or depress and release footswitch e The instrument carries out one complete section retraction stroke and the specimen head stops upon reaching the lower limit po sition While sectioning is in motion LED RUN is illu minated While the sectioning motor is deactivated LED STOP is illuminated Both LEDs are located in functional area C Instruction Manual V1 1 02 2000 5 Operation Functional areas A and C Instruments with sectioning motor only 5 4 2 Setting the sectioning window The term Sectioning window stands for a dif ferent slower speed setting in that part of the sectioning stroke where the specimen Is actual ly cut while the remaining sectioning stroke above and below the specimen as well as the retraction return stroke are carried out at a higher speed level time saving feature If a sectioning window Is set speed inside the window is as set on the control panel Outside the sectioning window the specimen head trav els at a proportionately higher speed Always set the sectioning window while in the sectioning stroke speci men moves downward Setting the window in retraction stroke speci men travels upward is not possible il e Position lower edge of specimen approx 3 mm above the knife edge rotate hand wheel clockwise Press sectioning window key
68. ual defrost cycle electric 320 W 3 185 g 5g refrigerant R 404A 0 4 alpha 22 Kyodo or RENISO E22 Fuchs Instruction Manual V1 1 02 2000 4 Instrument properties Refrigerant and compressor oil to be replaced only by trained and authorized service il personnel CM3050S 3 CM3050S 6 CM3050S 7 Microtome c UL a Rotary microtome 120V AC 10 208V AC 10 230V AC 10 Section thickness setting 0 5 300 um 60 Hz 60 Hz 60 Hz Overall specimen feed 25 mm 1800 VA 1800 VA 1800 VA Vertical stroke 59 mm 35 A eff 30 A eff 25 A eff Specimen retraction 50 um Maximum specimen size 40 x 55 mm T15A TI T12A T1 T10A T1 Specimen orientation 8 x y z axis 2 2 2 Trimming 6 thickness settings 5 150 um Il Il Motorized coarse feed 1800 J s 1800 J s 1800 J s slow 500 um s fast 1 000 m s Fluorescent light lamp cryochamber illumination 50 Hz version 60 Hz version Osram Dulux S 11 W 21 Light color LUMILUX hellweifs brilliant white Osram Dulux S 13 W 21 Light color LUMILUX hellweif brilliant white Cryocabinet Dimensions Weight Width w o handwheel 800 mm Width including handwheel 882 mm Depth 766 mm Height arm rest level 840 mm Overall height 1040 mm incl microtome w o specimen cooling ca 180 kg Leica CM3050 S Cryostat Sectioning motor Option Sectioning speed slow 0 1 mm s 100 mm s 210 mm s 0 1 mm s 170 mm s 21
69. uments Written statement confirming disinfection decontamination and method s applied to be included in shipment Hi If instruments parts for repair arrive at the Leica facility in a state which suggests that the instrument has not been properly disinfect ed decontaminated the instrument s part s will be returned to the customer at the customer s expense 8 3 Shutdown and disposal of the instrument 58 The instrument or parts of the instrument must be disposed of in compliance with the local laws The Leica CM3050 S contains a large number of recyclable components For more information on the recycling program for our cryostats please con tact your Leica sales representative or dealer or Leica Microsystems Nuss loch GmbH Germany We will be glad to provide you with details on the recycling concept for our cryostats which meets today s environmental requirements Instruction Manual V1 1 02 2000 Appendix 1 Electrohydraulic height adjustment An electrohydraulic lifting device for individual working height adjustment is available as an optional accessory for the CM3050 S cryostat series 1 1 Designated use The electrohydraulic height adjustment device has been designed exclusively for adjustment of the instrument working level to the individual height of the user lt has been designed for use with the Leica cry ostat series CM3050 S only and may under no circumstances be used to lift oth
70. us radioactive material Chapter 2 3 9 Leica CM 3050 S Cryostat 5 5 1 Freezing specimens onto specimen discs with quick freeze shelf and stationary heat extractor Size the specimen Apply sufficient amount of embedding medi um onto ambient temperature specimen disc Inside the cryochamber place the specimen onto the disc and orient Place specimen disc with specimen into one of the 10 openings in the quick freeze shelf see below and freeze The freezing process can be sped up further by using the stationary heat extractor 1 Next to the low temperature stabilizer 2 Parking Station 3 specimen discs 3 can be placed within its pivoting range Place the heat extractor 1 directly onto the specimen surface After approx 30 seconds the specimen Is completely frozen 39 5 Operation 5 5 2 Freezing specimens onto specimen discs with 90 prism Instruments w specimen cooling only In instruments with specimen cooling the 90 prism which ts inserted into the specimen head is an additional option to freeze specimens at maximum temperature Installing the 90 prism e Loosen screw 3 at specimen head e Insert pin 1 of prism completely into recep tacle 2 in the specimen head Important the entire rear surface of the prism must fit tightly against the specimen head surface e Retighten screw 3 at specimen head The prism now forms a horizontal surf
71. ve it from its socket 6 Installing the replacement lamp Hold the lamp in correct position see draw ing below and push towards the right until it locks into the socket Push the lamp slightly upwards until it locks into the clip Put the sliding window back in place Reconnect the instrument to mains and switch it back on Instruction Manual V1 1 02 2000 8 Warranty and service 8 1 Warranty Leica Microsystems Nussloch GmbH guarantees that the delivered product was subjected to stringent quality control to ensure that the instrument com plies with its technical specification The warranty conditions of your local Leica sales company or dealer apply Only authorized Leica technical service engineers may carry out repairs on the instrument Any intervention on modification of the instrument and any use of accesso ries other than those delivered by Leica is subject to prior approval by Leica unless explicitly specified otherwise in this manual Please note that any spare parts and accessories which are not supplied by Leica have neither been inspected nor have they been approved by Leica Therefore installation or use of such parts and or accessories may impair the design features of the instrument Leica assumes no liability for any damage caused by the use of non original spare parts or non original accessories Warranty claims can be made only if the instrument has been operated ac
72. witch instrument off remove accessories etc 54 7 5 2 Disassembling the specimen head instruments w specimen cooling only Use Allen key size 5 to remove screw 2 from the center of the specimen head 1 Do not twist the refrigerating tube see chapter 2 3 11 Removing reinstalling the microtome Hold hook 4 part of standard delivery in one of your hands and with your other hand rest refrigerating tube 3 on hook Insert hook 4 with refrigerating tube 3 into the opening above mark V Instruction Manual V1 1 02 2000 7 Disinfection cleaning and maintenance e Remove plug of temperature sensor 7 7 5 3 Reinstalling the microtome e Remove plug of coarse feed motor Pull bushing backward arrow 1 and hold Place your thumb between plug 6 and N When reinstalling the microtome socket atthe microtome arrow 2 make sure to observe the following To remove plug 6 from microtome pull it safety instructions away from the microtome while at the same time carrying out a slight jogging movement e Removing reinstalling arrows 3 the microtome Chapter 2 3 11 e Display message Dry Microtome Chapter 2 3 12 e Lock handwheel in lower position e Place microtome in chamber e Put one drop of cryostat oil per surface on the contact surfaces of plastic coupling 11 and handwheel coupling 12 e Place plastic coupling 11 on handwheel coupling
73. y possibly with hair dryer hairdryer only to be used outside the cryochamber or at ambient temperature Call Technical Service Specimen disc cannot be removed Specimen disc stuck to quick freeze shelf or specimen head due to moisture on underside of specimen disc Apply strong alcohol to contact point or heat the specimen head Cryostat inoperational Mains plug not properly con nected Automatic cut out has been triggered Check mains plug connection Reconnect automatic cut out switch instrument back on Sectioning motor inoperational Footswitch not connected Automatic cut out of sectioning motor has been trig gered Motor defective Connect footswitch Reconnect automatic cut out push inwards until it locks in place Call Technical Service No or insufficient refrigerating performance 50 Stopper not placed properly Into drain in bottom of cryo chamber Compressor defective Leak in cooling system Close drain with stopper Call Technical Service Call Technical Service Instruction Manual V1 1 02 2000 6 Troubleshooting applications tips 6 3 Reference chart for temperature settings in minus C Tissue 10 15 15 25 25 50 Adrenal Bone marrow Brain Bladder Breast fatty Breast little fat Cartilage Cervical sk Fatty Heart and vascular Intestinal Kidney Laryngeal Lip Liver Lung Lymphoid

Leica CM3050 S Cryostat Manual

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