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PowerPlex 16 System DC6530 DC6531 products User Manual

1. DNA CER AI CE Ladder Ladder Ladder Gold ST R 1xBuffer Hi Di Ladder Ladder Wk Ladder Ladder Ladder
2. CE 10x Fis SK BRIA EKE TE DNA fi 2ng ja sulle 36 010 58256268 010 58256160 100013 HUTTE 3 09 TMD012 o 45 54 i e ABI PRISM 31038 fe 4 Wr DUE 377 DNA C RA ARL o Promega e V 15 Tra d
3. 2 3 VILE fi HiGeneScan 4t fiMacintosh 1 GeneScan 2 Sample raw data X 3 TRA CIEE 36 B 907 909
4. 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 3 omege Promega PowerPlex 16 AmpliTaq Gold DNA BNA STR Perkin Elmer 480 GeneAmp PCR 9600 9700 2400 PowerPlex 16 Figure 1 Promega STR STR H www pr
5. HKZ GR 36 B 907 909 010 58256268 010 58256160 100013 WWW promega com cn 3 09 CTMD012 50 o Promega PowerPlexe 16 System 9 Nae ie EFT T HE PowerPlexe 16 System D21S11 n 2 n 2 2 2 Low level products D7S820 D13S317 D3S1358 TH01 JOE 270 271bp Ladder off ladder artifact TPOX 8 26
6. TRB CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 22 1 ILS 1ul ILS 600 x HE ILS 600 amp 24ul Hi Di x o Promega RiHi Di BI ALS ILS 100bp 500 1000 RFU itl ILS 600 24 5Hl 0 5bHl 2 10 15s 3 F25ul FR LS 1Hl
7. Module Manager run module Gold ST R 1xBuffer DNA 2 4 5 6 95 C 3 3 SABI PRISM 3100 ABI PRISM 3100 GeneScan36_POP4DefaultModule 2000 3 kV 11 Module Manage
8. 45 V C ABI PRISM 310 95 C e 31 OE 4N ERE 47cm x 50um e Performance Optimized Polymer 4 POP 4 Am e 008 F EDTA 10x Hi Di iti Applied Biosystems Cat 4311320 e PowerPlex Matrix Standards 310 Cat DG4640 Ikk EEH P 100uS cm Hi Di 20 C 4 C 100hS cm DNA RH
9. PowerPlex 16 Allelic Ladder Mix Internal Lane Standard 600 DNA ART tips IX 75 STR Table 1 Table 1 ABI PRISM 310 3100 3100 Avant Applied Biosystems 3130 3130Xxj F BUE ABI PRISM 377 DNA Long Ranger 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 8 TEL Blue Dextran
10. Gold STR 1xBuffer DNA 2 4 10 18 10 20 4 1 0hl PowerPlex 16 Allelic Ladder 2 0Hl Allelic Ladder 5 6 95 C 3 7 15 20 Pause 8 18 60cc 1 5H HH 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn
11. ILS600 e e Ladder GeneMapper ID Ladder Ladder Project DVI B VI C STR Pane STR Panel 36 B 907 909 010 58256268 010 58256160 100013 3 09 www promega com cn 57 CIMD012 Ladder Ladder Allelic Ladder
12. STR STR PCR STR STR PCR 3 A 60C 30 TIV B 13 36 B 907 909 010 58256268 010 58256160 100013 3 09 www promega com cn 53 CTMD012 o Promega A CHE TV L150 CCE CR
13. 20 Filter Macro AHA DU BE EEE Genotyper2 2 5 3 6 Edit ITRE Macro HY Check ILS Genotyper 2 5 3 6 Edit Ladder CE CE PowerTyper 16 Macro 2 0 Ladder Ladder GeneScan 36 B 907 909 010 58256268 010 58256160 100013
14. Gold ST R 1xBuffer DNA 10 20 4 25HI 5 HR Ls WEULSTR 4 2E bili 5 1ul PowerPlex 16 Ladder 2 4 10 18 2 A UB 6 95 C 3 3 7 8 Ll 48 96 TRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 Lj CTMD012 823 o Promega
15. Classic Z Basic or Advanced 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 58 CTMD012 Panel Promega Panel STR Panel Ladder Both the Bin Set used in the Method and must belong to the same Chemistry Kit Analysis the Panel Bin GeneMapper Manager Analysis Method
16. 18 7 PreRun 15 20 40 C 8 ladder TRA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 5828 o Promega 1 ILS 600 Blue Dextran 0 5ul ILS 600 x 1 5ul Blue Dextran x 2 10 15s 3 1 0Hhl 2 0HI
17. 36 B 907 909 010 58256268 010 58256160 100013 3 09 55 WWNW promega com cn CTMD012 DNA Ri FTA o Promega 1ng 2 4 10 20 10 18 2 4 10 20 10 18 DNA DNA ERD H DNA yr i _DNA DNA 10xPrimer Pair Mix Gold STX R 10xBuffer 15 10xPrimer Pair Mix
18. DNA DNA CO 2 4 10 20 10 18 5 PCR 6 PCR DNA 0 5 1 ng 7 9947A DNA 0 5ngDNA 0 5ngDNA 9947A PCR 8 DNA PCR 9 GeneAmp92 PCR System 9600 9700 2400 MicroAmp 480 GeneAmp 1
19. Analysis Module 1 No Selection GeneScan 12 OK 13 14 15 16 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 21 Promega Run nstrument 17 Run Instrument 18
20. Taq DNA DNA 3 60 C 30 DNA FGA D21S11 PentaE Table 5 Table 4 PowerPlex 16 TRA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 66
21. PowerPlex8 16 System Genotypere PowerPlexs 16 System ABI PRISM 310 3100 1 0 1 1 1 ABI PRISM 377 DNA GeneScan Genotypere PowerTyper 16 Macro 2 0 Promega PowerTyper 16 Macro 2 0 gt PowerTyper 16 Macro 2 0 PowerTyper Y Macros CD ROM Cat DG3470 www promega com geneticidtools PowerTyper 16 Macro 2 0 ii 5 Macintosh Genotyper 2 5 Windows NT Genotyper 3 6 PowerTyper 16 Macro 2 0 Genotypere sample info Macintosh tii color info Windows NT Ladder ladder Macro ladder Ladder PowerTyper Macro
22. 1 MicroAmp8 2 GeneAmp PCR system 9600 9700 2400 Perkin Elmer 480 3 20 C 4 C 4 C TRA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 13 o Promega Protocol for the GeneAmp PCR Protocol for the GeneAmp PCR System 9700 Thermal Cycler System 2400 Thermal Cycler 95 C for 11 minutes then 99 C for 11 minutes then 96 C for 1 minute then 96 C for 1 minute then ramp 100 to 94 C for 30 seconds ramp 100 to 94 C for 30 seconds ramp 29 to 60 C for 30 seconds ramp 100 to 60 C for 30 seconds ramp 23 to 70 C for 45 seconds ramp 23 to 70 C for 45 seconds for 10 cycles then for 10 cycles then ramp 100 to 90 C for 30 seconds ramp 100 to 90 C for 30 seconds ramp 29 to 60 C for 30 seconds ramp 100 to 60 C for 30 seconds ramp 23 to 70 C for 45 seconds ram
23. 20 DNA K Na Mg EDTA PCR PH PCR DNA TE 10mM Tris HCL 0 1mM IV B 13 36 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 52 o Promega 10xPowerPlex 16 15 ILS600 u xiki MAE HI Di Hi WIE DNA
24. Ladder Genotyper 2 5 3 6 36 B 907 909 010 58256268 010 58256160 100013 3 09 61 www promega com cn CTMD012 o Promega Edit f ove complete HK Could not the Run Macro command because the labeled peak could not be found Ladder Ladder 150RFU 150RFU Ladder 150RFU Macro Ladder CE Ladder Ladder GeneScan X heavy smoothing TH01 9 3 10
25. Ti ot Wr BM Be E N light smoothing Ladder m PowerTyper PowerTyper Y Macro Ladder At E Nu f A GeneScan categories UR category 6bp Macro 36 B 907 909 010 58256268 3 09 010 58256160 100013 62 WWNW promega com cn CTMD012 Ladder o Promega Ladder Ladder
26. 1 ILS Hi Di 0 5pl ILS 600 x 9 5ul Hi Di x ILS ILS 100bp 500 1000 RFU ISL 9 0hl 1 0bIILS 600 ILS 600 9 75hl 0 25yl ILS 600 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 19 2 iit 13x12 10 158165 3 10yl ILS 4 1hl 1hl Ladder
27. 15 B ABI PRISM 3100 Version 1 0 1 1 1 DR OE 18 C ABI PRISM 310 22 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 1 D JH ABI PRISM 377 DNA WO Mic MINI III I I EU 30 A GeneMapper ID Version 3 2 PowerPlex Panel Bin 30 B GeneMapper ID Casework 4 PTEEIT Less 32 C GeneMapper ID 38 D GeneScan PC BEVE SSZEUETTREASA IT sess 41 E GeneScans Macintosh 43 F Genotyper PowerTyper 16 Macro 45 Fe HH 48 EI 49 RE a E T 51 Pe ee ae se eect cece cana T T 51 B GeneMapper ID 4 57 C PowerTypar 16 Macro 61 VI wa MeR 64 Ds WN RR INTTR E E E EE EEA 65 A STR B PowerPlex 16
28. Alleles Bin Alleles Bin Bin Figure 3 e ABI PRISM 3100 3100 A vant 2 33 48 4 Wr 4 Applied Biosystems 3130 3130X e ABI PRISM 310 Matrix Matrix 36 B 907 909 010 58256268 3 09 010 58256160 100013 www promega com cn 59 CTMD012 Classic Mode o Promega XJ FE Js fi Hl Classic Bi xt UE Basic or Advanced Advanced ABI PRISM 310 Some selected sample s do not contain analysis data T
29. 150 27 32 STR Table 7 PowerPlex 1 22 PowerPlex 16 PowerPlex 16 1 1 41x1018 PI 4 1 1 83 10 Table 8 PowerPlex 1 2 PowerPlex 16 PowerPlex 16 500000 Table 9 PowerPlex 16 0 999998 Table 7 PowerPlex 1 2 PowerPlex 16 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012
30. 2 0 2mm 4 3 Table 3 50ml 5 Long Ranger Table 3 5 Long Rangere 6M ES 10xTBE 1x 50 Long Ranger Ez ER 5 PRA B CIEE 36 B 907 909 010 58256268 010 58256160 100013 3 09 26 WWNW promega com cn CTMD012 50ml YER Long Ranger Singel Pack 0 2 Nalgene9 5 5 50ml 35yl TEMED 250pl 10 tx RRE E 6 60cc 7
31. 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 20 Promega 3 22 1 3kV 5 GeneScan36_POP4PowerPlex16_3kV_11secs_ 2000 6 GeneScan 96 well Finish 7T sample name color info ladder color info adder PowerTyper 16 Macro 2 0 8 BioLIMS Project 3100_Project1 9 Dye Set Z 10 ABI PRISM 3100 1 0 1 1 1 Run Module 1 GeneScan36_POP4PowerPlex16_3kV_11secs_2000 11
32. 4 mU I AMET D DNA E 014 5 200 deta ne ee ns eo dy ee nasan F PowerPlex 16 G pp 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 2 SN EIDA AN AE 74 75 STR 3 7 1 4 STR PowerPlex 16 16 15 STR 1 Penta E D18S51 D21S11 THO1 D3S1358 FGA TPOX D8S1179 vWA Amelogenin Penta
33. PCR PCR TRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 12 PCR IV B Perkin Elmer 480 1GeneAmp PCR system 9600 9700 2400 PowerPlex 16 System e mail Promega Chinatech promega com Promega DNA 0 5 1ng 10 22 DNA FTA 10 16 10 18 10 20
34. Sample info Views show dye lanes window TAZ CIEE 36 B 907 909 010 58256268 42I 010 58256160 100013 www promega com cn 3 09 CTMD012 45 o Promega 1 PowerTyper Macros CD ROM Cat DG3470 Promega PowerTyper 16 Macro 2 0 2 Genotypere PowerTyper M 16 Macro 2 0 Genotype Genotyper Analysis Software User s Manual 3 File Import YER Edit Set Preferences 4 Check ILS ILS 600 GeneScan Lad
35. DNA FTA DNA DNA DNA IQ System PowerPlex 16 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 71 o Promega X AluQuant Human DNA Quantitation System Cat DC1010 DNA IX DNA IQ System AluQuant Human DNA Quantitation System Beckman Coulter Biomek 2000 Beckman Coulter Promega www promega com worldwide E mail Chinatech promega com IX E ILS600 WER CLS 22 DNA 60 80 100 120 140 160 180 200 225 250 275 300 325 350 375 400 425 450 475 500 550 600
36. O 100pS cm Hi Di 20 C 4 C 100hS cm DNA One FE RI ERI BMA Hl ERE SLA CI SCE IE IER SBE TBA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 15 o Promega 1 0 5ul ILS 600 x YER ILS 600 0 25HIILS 600 CLS 6000 2 10
37. 6 ABl PRISM 310 ABI PRISM 377 DNA Matrix Matrix 7 Analyze VIC GeneMapper D Tool GeneMapper Manager 2 Analysis Methods 3 New 4 HID OK YER HID GeneMappere ID email Chinatech promega com Promega 5 PowerPlex16_20 ofilter 6 Allele 7 Bin PowerPlex System 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 38 o Promega Promega_Bins_ID3 2 X X Bin 8
38. OK 5 Size Standard Editor Figure 6 ILS 600 advanced 6 Red 7T IX E Figure 13 72 8 OK 1 Applied Biosystems GeneMapper ID Software Human Identification Analysis Tutorial rx Project 2 frSample Type Ladder Sample Positive Control Negative Control Ladder 3 Analysis Method Creating a Databasing or Paternity Analysis Method 4 Panel PowerPlex_16_ID3 2 X X Panel VI A 30 Panel 5 Size Standard Creating a Size Standard 6 ABl PRISM 310 ABI PRISM 377 36 B BE 907 909 010 58256268
39. www promega com tbs IV PowerPlex 16 DNA Thermal Cycler Model 480 GeneAmp PCR System 9600 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 9 o Promega 9700242400 Applied Biosystems 0 5ml 0 2ml MicroAmp 96 Applied Biosystems 1 5ml Fisher Cat 05 402 26 IX 1 55 AmpliTaq Gold DNAX Zril Applied Biosystems Cat P1193 Fy Cat DY1151 Thermal Cycler Model 480 25HI 0 5 1ng DNA GeneAmp PCR System 97004634 Xt
40. 2 1 2 PCR 3 0 2m 0 5ml MicroAmp JER GeneAmp PCR System 9600 9700 2400 MicroAmp88 MicroAmp Perkin EImer model 480 0 5ml GeneAmp 4 PCR Table 2 1 5ml o Table 2 IX F 53 PCR n o Table 2 PowerPlex 16 PCR
41. 59 C 60 C DNA 36 B 907 909 010 58256268 010 58256160 100013 3 09 56 www promega com cn CTMD012 VII B GeneMapper ID o Promega GeneMapper ID GeneMappere ID Ladder OL analysis type ds iE Basic or Advanced Figure 11 ILS600 ILS600 ILS ar BER Ladder
42. PentaE PentaD PowerPlex 16 PentaE PentaD DNA PowerPlex 16 6 TRA CERO 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 65 o Promega PowerPlex 16 DNA STR Tagq DNA n 4 DNA DNA AE DNA
43. 34 0 2mm WERKA ERIX Liqui Nox8 HAZ CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 25 PowerPlex Matrix Standards 310 Cat DG4640 Blue Dextran Loading Solution Cat DV4351 95 C o Promega Long Rangere ABI PRISM 377 DNA 36cm 1 1 Liqui Nox
44. 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 36 wre SF Mudard Editos Figure 6 6 Red 7 IX E 72 Figure 13 8 OK Casework 1 Applied Biosystems GeneMapper ID Software Human 36 5 ee 909 010 58256268 010 58256160 100013 WWW Dro i 3 09 CTMD012 37 o Promega Identification Analysis Tutorial rx Project 2 frSample Type CLadder Sample Positive Control Negative Control Ladder 3 Analysis Method Creating a Casework Analysis Method 4 Panel PowerPlex_16_ID3 2 X X Panel VI A Panel 5 Size Standard Creating a Size Standard
45. 36 34 64 96 8 3 9 10 2 1 ABI PRISM 377 2 GeneScanm sample info PowerPlex 16 Ladder sample info ladder PowerTyper 16 Macro 2 0 3 GeneScan 36 B 907 909
46. ladder TRB CERO 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 24 o Promega Matrix B 6 auto analyze AB PRISM 310 Run 40 V D ABI PRISM 377 DNA IX H Long Rangere Cambrex Cat 50611 ABI 377 36cm Long Ranger Singel pack Cambrex Cat 50691 10 IMRE Cat V3131 TEMED Cat V3171 TBE 10xbuffer Nalgene 0 2 36cm 36cm 0 2mm JE 36
47. 31004 FEE 36cm 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 18 Promega e 3100 Performance Optimized Polymer 4 POP 4 EDTA 10x e 3100 MicroAmp 96 FLL AUIS iti Hi Di iti Applied Biosystems Cat 4311320 e PowerPlex Matrix Standards 3100 3130 Cat DG4650 Q 100hS cm Hi Di HERE 20 C 4 C 100hS cm EBKEN DNA ERE EF OR
48. POWER 2 Ladder POWER 3 Ladder Ladder Ladder Off Ladder Ladder lili n 7 Display Fluorescein Data 8 Display TMR Data 9 Display JOE Data 10 Power Table Make Allele Table Make CODIS Table 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 47 o Promega 3 Power T
49. 2 DNA 25hl IX G PCR IX H 74 e TAE 1x 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 73 e Dae e 5x e 0 5pg ml 1 100ml 1xTAE 2g 2 150cm2 60C 2 55C 820 304 Ph 3 10Hl 2 5h 5x 4
50. o Promega GenBank Locus and Repeat Sequence SIRLocus Label Chromosomal Location Locus Definition 5233 Penta E FL 15q NA AAAGA D18551 FL 18q21 3 HUMUT574 AGAA 23 D21511 FL 21911 21921 HUMD ILOC TCTA Complex 23 THO1 FL 11p15 5 HUMTHO1 human tyrosine AATG 23 hydroxylase gene D351358 FL 3p NA TCTA Complex FGA TMR 4028 HUMFIBRA human TIIG fibrinogen alpha chain gene Complex 23 TPOX TMR 2p23 2pter HUMIPOX human thyroid AATG peroxidase gene D8S1179 TMR 8q NA TCTA Complex 23 vWA TMR 12p12 pter HUMVWFA31 human von TCTA Willebrand factor gene Complex 23 Amelogenin TMR Xp22 1 22 3 and Y HUMAMEL human Y NA chromosomal gene for Amelogenin like protein Penta D JOE 21q NA AAAGA CSFIPO JOE 5q33 3 34 HUMCSFIPO human c fms AGAT proto oncogene for CSF 1 receptor gene D165539 JOE 16q24 qter NA GATA D75820 JOE 7911 21 22 NA GATA D135317 JOE 1322 931 NA TATC D55818 JOE 5q23 3 32 NA AGAT The August 1997 report 24 25 of the DNA Commission of the International Society for Forensic Haemogenetics ISFH states 1 for STR loci within coding genes the coding strand shall be used and the repeat sequence motif defined using the first possible 5 nucleotide of a repeat motif and 2 for STR loci not associated with a coding gene the first database entry or original literature description shall be used lt Amelogenin is not an STR but displays a 106 base X specific band and a 112 base Y specific band 9947A DNA female
51. Use marker specific stutter ratio if available 9 PowerPlex 16 System Figure 7 Stutter Applied Biosystems nstallation Procedures and New Features for GeneMapper ID Software 3 2 Analysis snthord bor HID Garami Aen Pom Detecter Pes Gustty Quatty Pags Wn Sen freemeta Bo IIT 7 Une materspeecifeo abutter iue H avislable Mate Repast Type Cut off Valve T T Minur Shotts Rate bo bs pm E p ini ini ini Figure 7 20 Allele Promega_Bins_ID3 2 X txt X Bin 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 39 Creating a Size Standard 1 Tool GeneMapper Manager 2 Size Standard 3 New 4 Basic or Advanced Figure 5
52. 70 o Promega Matching Probability STR System African American Caucasian American Hispanic American Asian American PowerPlex 1 2 System 8 STR loci 1 in 2 77 x 108 1 in 1 15 x 108 1 in 1 45 x 108 1 in 1 32 x 108 PowerPlex 16 System 15 STR loci 1 in 1 41 x 1015 lin 1 83 x 1017 1 in 2 93 x 107 1 in 3 74 x 107 Table 8 Typical Paternity Indices of the PowerPlex 1 2 and 16 Systems in Various Populations Typical Paternity Index STR System African American Caucasian American Hispanic American Asian American PowerPlex 1 2 System 497 262 318 471 PowerPlex 16 System 2 510 000 1 520 000 522 000 4 110 000 Table 8 PowerPlex 1 2 PowerPlex 16 Power of Exclusion STR System African American Caucasian American Hispanic American Asian American PowerPlex 1 2 System 0 9982042 0 9968863 0 9973367 0 9981793 PowerPlex 16 System 0 9999996 0 9999994 0 9999983 0 9999998 IX D DNA DNA IQ System Cat DC6700 DNA STR DNA IQ System DNA PCR DNA IQ System
53. 3 2 Applied Biosystems GeneMapper ID Software Human Identification Analysis Tutorial 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 30 Promega GeneMappere ID 3 1 3 2 1 GeneMapper D Promega www promega com geneticidtools panels bins Panel Bin 2 GeneMapper ID version 3 2 Submit 3 PowerPlex Panels amp Bin Sets zip 4 Windows WinZip Panel Bin Applied Biosystem GeneMapper ID 1 4 1 GeneMapper ID 3 2 2 Tool Panel Manager 3 Panel Manager
54. CXR PowerPlex 16 4 ILS 600 PowerPlex 16 ILS 600 V 120 140 160 180 225 250 275 sie Figure 12 ILS600 IX F PowerPlex 16 TRA CIEE 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 72 So Table 10 PCR CUD bl Table 10 PowerPlex 16 Volume Per Numberof Final Volume PCR Master Mix Component Reaction Reactions ul Gold ST R 10X Buffer 2 5pl x PowerPlex 16 10X Primer Pair Mix 2 5pl x AmpliTaq Gold DNA polymerase 0 8ul 4u x nuclease free water ul x Per tube x template DNA volume 0 25 1ng up to19 2yl x total reaction Volume 25ul x 1 AmpliTaq Gold DNAX A WERE Sul TiNE TKIRCAZIEZEL6 DLE EE A AR
55. 010 58256160 100013 www promega com cn 3 09 CTMD012 40 Promega DNA Matrix Matrix 7 Analyze VI D GeneScan PC 1 GeneScan 2 Sample raw data X 3 Figure 8 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 41 P PowerPlex 16 Analysis gep nsyns Range Size Call Range C Ful Range Ful Range G Thus Range Data Poris C Tha Ringe aie Paes Stet Eo Mie Stop hooo ME frou ee 2 Pelynemisibegee b Ped Window Size ie Pw Sipe Taratata for Do Pos Start Shpa Three tor Fo Pas End
56. ARER 1 ABI PRISM 310 2 AB PRISM 310 GeneScan sample info PowerPlex 16 Ladder sample info ladder PowerTyper 16 Macro 2 0 3 4 GS STR POP4 1ml A 3 30 Inj Secs 3 Inj kV 15 0 Run kV 15 0 Run C 60 Run Time 30 O eee DNA 1ng 2 5 YER ABI PRISM 310
57. Figure 8 VI D 2 41 4 Params C AppliedBio Shared Analysis Sizecaller Params 5 6 size standard size standard Define New IX E 72 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 42 o Promega Figure 13 Size Standards C AppliedBio Shared Analysis Sizecaller SizeStandards 7 PowerTyper 16 Macro 2 0 GeneScane VI F 45 1
58. 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 43 Analysis Range Data Processing Peak Detection Size Call Range Size Calling Method Split Peak Correction Stop 10 000 Baseline Checked Multicomponent Checked Smooth Options Light Peak Amplitude Thresholds B Y G R Min Peak Half Width 2pts Min 60 Max 600 Local Southern Method None o Promega Start Defined in Step 2 1 heavy smoothing TH01 9 3 10 50 200 RFU peak amplitude thresholds 4 Params 5 6 size standard size standard Define New X E Figure 13 Size Standards 7 PowerTyper 16 Macro 2 0 GeneS
59. 78 o Promega a STR loci are the subject of U S Pat No RE 37 984 German Pat No DE 38 34 636 C2 and other patents issued to the Max Planck Gesellschaft zur F rderung der Wissenschaften e V Germany The development and use of STR loci are covered by U S Pat No 5 364 759 Australian Pat No 670231 and other pending patents assigned to Baylor College of Medicine Houston Texas Patents for the foundational PCR process European Pat Nos 201 184 and 200 362 expired on March 28 2006 In the U S the patents covering the foundational PCR process expired on March 29 2005 b U S Pat Nos 6 238 863 and 6 767 703 and Korean Pat No 691195 have been issued to Promega Corporation for materials and methods for identifying and analyzing intermediate tandem repeat DNA markers Other patents are pending c U S Pat Nos 5 843 660 6 479 235 6 221 598 and 7 008 771 Australian Pat No 724531 Canadian Pat No 2 118 048 Korean Pat No 290332 Singapore Pat No 57050 and Japanese Pat No 3602142 have been issued to Promega Corporation for multiplex amplification of STR loci Other patents are pending d The purchase of this product does not convey a license to use AmpliTaq Gold amp DNA polymerase You should purchase AmpliTaq Gold amp DNA polymerase licensed for the forensic and human identity field directly from your authorized enzyme supplier 2000 2008 Promega Corporation All Rights Reserved Ma
60. 3 22 seconds 1 3 Protocol Manager New Type Regular Run Module Dye Set F OK Plate Manager Application GeneMapper Generic 96 well OK TER 4 GeneMappere Results group 1 Instrument HKZ GR 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 17 Promega Protocol 1 2 OK YER
61. STR DNA DNA Southern Promega STR Allelic Ladder PowerPlex 16 ja IX B PowerPlex 16 PowerPlex 16 Table 4 Table 5 CFBD 13 STR CODIS DNA PowerPlex 16 CODIS PowerPlex 16 5
62. 100ml Gold ST R 10X Buffer 500mM KCI 100mM Tris HCl pH 8 3 at 25 C 15mM MgCl2 1 Triton X 100 2mM each dNTP 1 6mg ml BSA TAE 50X buffer pH 7 2 242g Tris 57 1ml 100ml 0 5M EDTA Tris EDTA 500ml 1L TBE 10x 107 8g Tris 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 75 744g EDTA Na EDTA 2H O 55 0g WRR Tris EDTA 800ml PH 8 3 1 TE 10mM Tris HCl 0 1mM EDTA pH 8 0 2 21g Tris tik 0 037g EDTA Naz2EDTA 2H20 Tris EDTA 900ml HCI PH 8 0 1 IX I
63. 3 09 CTMD012 29 o Promega Do 1 0 2 0hl 10 1 Cancel run time X 3 Run 2 3 3 600bp 4 TH 1 Liqui Nox VER 2N HCI 15 VI VI A GeneMapper ID 3 2 PowerPlex Panel Bin PowerPlex 16 System GeneMapper D 3 2 Panel Bin T fH GeneMapper D
64. Reference Sample Kit for Maxwell 16 48 preps AS1040 DNA IQ Casework Sample Kit for Maxwell 16 48 preps AS1210 Plexor HY System 800 reactions DC1000 200 reactions DC1001 Slicprep 96 Device 10 pack V1391 TRA CERO 36 B BE 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 77 o Promega Product Size Cat Ammonium Persulfate 25g V3131 TBE Buffer 10X 1L V4251 Urea 1kg V3171 Blue Dextran Loading Solution 3ml DV4351 ART Aerosol Resistant Tips Product Volume Size tips pack Cat ART 10 Ultramicro Pipet Tip 0 5 10yl 960 DY1051 ART 20E Ultramicro Pipet Tip 0 5 10yul 960 DY1061 ART 20P Pipet Tip 20yul 960 DY1071 ART GEL Gel Loading Pipet Tip 100ul 960 DY1081 ART 100 Pipet Tip 1001 960 DY1101 ART 100E Pipet Tip 100ul 960 DY1111 ART 200 Pipet Tip 200u1 960 DY1121 ART 1000E Pipet Tip 1 000u1 800 DY1131 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012
65. 18 2yl Gold STX R 10xBuffer 2 5yl PowerPlex 16 10xPrimer Pair Mix 2 5ul AmpliTaq Gold DNAR i i 0 8ul DNA 0 5 1ng hl 1 0ul 25ul 1 PCR Gold STAR 10xBuffer 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 11 o Promega PowerPlex 16 10xPrimer Pair Mix AmpliTaq Gold DNAR Zril 7627986 JL ADNABE 2 AmpliTaq Gold DNA 5u pl PCR 3 DNA TE 4 10mM Tris HCI pH 8 0 0 1mM EDTA DNA TE PH 8 0 DNA PCR 20 PCR PH Tris HCI EDTA PCR
66. 4 File Import Panels 5 Panel Bin Promega_Panels_ID3 2 X txt X Panel Bin Import 6 Promega_Panels_1D3 2 X 7T File Import Bin Set 8 Panel Bin Promega_Bins_ID3 2 X txt Import 9 Panel Manager Apply OK Panel Manager 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 31 o Promega VI B GeneMappere D Casework Applied Biosystem GeneMapper D 1 11 1 Tool GeneMapper Manager 2 Analysis Methods 3 New 4 HID OK VER HID GeneMapper D email Chinatech promega
67. 60 CIMD012 VII C PowerTyper 16 Macro o Promega HID GeneMapper Manager General HID Genotyper Genotyper 2 5 Macintosh Genotyper 3 6 Windows NT Genotypere PowerTyper 16 Macro Genotyper2 2 5 CD ROM E mail Chinatech promega com CD ROM could not Ladder complete the Run Ladder Macro command because no dye lanes are selected sample info color infor ladder Macro ladder
68. VIRES Mos Pea Hai miga f ed e5ssthosg C nene G uen ee Cages b C nay Fem Window Site s a Pispa Theaphoid Bact c Mo dme t pm ATER bo SC aihoq Mered Pea End fo Figure 4 Peak Detector 11 Peak Quality Peak Quality 12 Quality Flags 11 12 GeneMappere D 13 OK Creating a Size Standard 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 35 1 Tool GeneMapper Manager Size Standard New B N Basic or Advanced Figure 5 OK Select Dye and Analysis Method CO Dawe ec Advice d O Oum Figure 5 Select Dye and Analysis Methed7J Zi 5 Size Standard Editor Figure 6 ILS 600 advanced TRA CIEE
69. results group New General tab Analysis tab Analysis type GeneMapper Generic 5 6 spectral viewer dye set F dye set F 7T 3 4 8 9 Run Instrument 10 Run Instrument 11 45 V B Version 1 0 1 or 1 1 ABI PRISM 3100 95 C
70. 15s 3 10h LS 4 1Hl 1hl Ladder i A Abr CLS 600 Hi Di 9 5pl Hi Di x ILS 100bp x E 500 1000 RFU ISL 9 0Hl 1 0Hl VURUILS 600 9 75Hl FH Er FH USUS us Manager run module K i EHER Gold STXR 1xBuffer DNA 5 Module EF ZUR FY Eg BORA IA
71. Avant Applied Biosystems 3130 3130x PowerPlex Matrix Standards 3100 3130 IX 75 WI MLA 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 7 PCR 11 12 DNA PCR DNA PCR STR DNA Gold STR 10xBuffer and PowerPlex 16 10xPrimer Pair Mix
72. Loading Solution III B Matrix ABI PRISM 310 3100 3100 Avant Applied Biosystems 3130 3130xf Matrix Matrix PowerPlex Matrix Standards 310 Cat DG4640 ABI PRISM 310 ABI PRISM 377 DNA Matrix ABI PRISM 310 PowerPlex Matrix Standards 3100 3130 Cat DG4650 Matrix PowerPlex Matrix Standards 3100 3130 Cat DG4650 ABI PRISM 310 3100 3100 Avan Applied Biosystems 3130 3130x PowerPlex Matrix Standards 310 Cat DG4640 Matrix Matrix DG4640 PowerPlexe Matrix Standards 310 RFH STBDO21 DG4650 PowerPlex Matrix Standards 3100 3130 HERF BD022 Promega Fi
73. PowerPlex 16 GeneAmp PCR System 9600 9700 2400 Perkin EImer model 480 IV A o VILLA 37 1 Gold STXR 10xBuffer PowerPlex 16 10xPrimer Pair Mix 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 10 CD 15 10xPrimer Pair Mix 2 Gold STXR 10xBuffer 37 C
74. STR Product PowerPlex 16 Monoplex System Penta E Fluorescein PowerPlex 16 Monoplex System Penta D JOE PowerPlex ES Monoplex System SE33 JOE PowerPlex 1 2 System PowerPlex 16 BIO System PowerPlex ES System PowerPlex Y System Size 100 reactions 100 reactions 100 reactions 100 reactions 100 reactions 400 reactions 100 reactions 400 reactions 50 reactions 200 reactions Cat DC6591 DC6651 DC6751 DC6101 DC6541 DC6540 DC6731 DC6730 DC6761 DC6760 36 B 907 909 010 58256268 010 58256160 100013 3 09 76 www promega com cn CTMD012 o Promega Product Size Cat PowerPlex Matrix Standards 310 50ul each dye DG4640 PowerPlex Matrix Standards 3100 3130 25ul each dye DG4650 PowerTyper Macros 1 CD ROM DG3470 Internal Lane Standard 600 150yl DG2611 Gold ST R 10X Butfer 1 2ml DM2411 Mineral Oil 12ml DY1151 Nuclease Free Water 50ml 2 x 25ml P1193 Product Size Cat DNA IQ System 100 reactions DC6701 400 reactions DC6700 Ditterex System 50 samples DC6801 200 samples DC6800 Maxwell 16 Instrument each AS2000 DNA IQ
75. VILA DNA H styl lix E TRA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 51 Promega DNA BUSES DNA _ N HW Low copy number LON ILS600 AmpliTaq Gold DNAR ATi AFi PH DNA TE EDTA DNA
76. 00013 www promega com cn 3 09 CTMD012 46 o Promega GeneScan2 Genotypere Ladder ALS Ladder Ladder ILS 6 Allelic Ladders Fluorescein Ladders Penta_E D18S51 D21S11 TH01 D3S1358 JOE Ladders Penta_D CSF1PO D16S539 D78820 D13S317 D5S818 JOE Ladders Penta_ D CSF1PO D168539 D7S820 D13S317 D5S818 TMR Ladders FGA TPOX D8S1179 vWA Amelogenin Ladders Ladder Macro Ladder
77. 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 27 o Promega Plate Check Module Plate Check A PreRun Module PR GS 36A 2400 Run Module GS 36A 2400 Collect time 3 hours Well to Read distance 36cm 4 5 Matrix B 9 1 2 1 2mm 3 377 4 AB PRISM 377 DNA4 5 1xTBE 6 60cc
78. 1 TAE 1x 5 0 65cm 6 5x Step 3 7 5v cm 2 8 0 5yg ml 1xTAE 20 20 9 302nm TER PCR IX H 102631 Bi Re EK 0 05g 500HI TRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 B74 Promega Blue Dextran 88 25 15mg ml Blue Dextran 4 1mM EDTA PH8 0 CYOpg mD 10g
79. 1 111 D55818 12 11 11 11 13 11 Information on strains 9947A 9948 and K562 is available online at locus umdnj edu nigms Strain K562 is available from the American Type Culture Collection www atcc org Manassas VA Strain K562 displays three alleles at the D21S11 locus 3 Strain 9948 displays three alleles at the CSF1PO locus The peak height for allele 12 is much lower than those for alleles 10 and 11 IX C PowerPlex 16 15 STR HKZ GR 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 69 o Promega Table 7 9 The Bode Technology Group Springfield VA Raleigh NC Palm Beach West Palm Beach FL Virginia Division of Forensic Science Richmond VA Charlotte Mecklenburg NC 200
80. 3 09 63 www promega com cn CTMD012 aspie File CHE 2728 M8 2004 04 1T Coe Sample Origin Path Gi Puiveace Technical Jeqvice lel dactatgqereticeVCRE LTIh BG 2004 06 17 fee Status Message t Champed sire standard from 101s80500udv to ILEO Clasric Pile Seurce t Dist media Birra Must sage MgereticauCRE ITIR O0 2004 0 17 fpa KEither Panel Size Standard oc Tis Rathod was i1mvwlid J Suu le Type 3e 5685TA Figure 11 GeneMappere ID mien ee ee on mre iriri Senne Ga m 1 ES EE EEtiitiii SSeS 8 less B96TA Figure 12 GeneMapper ID VIN BA SCHR CHG 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 64 o Promega www promega com geneticidentity STR IX IX A STR AMP FLP VNTR STR 500bp DNA
81. 8256268 010 58256160 100013 www promega com cn 3 09 6 CIMD012 o Promega 20 C PowerPlex 16 10xPrimer Pair Mix PowerPlex 16 Allelic Ladder Mix Internal Lane Standard 600 Blue Dextran Loading Solution 3ml DV4351 PowerTyper Macros Release 2 0 1 CD ROM DG3470 Promega Genotyper PowerTyper Macros 2 0 CD ROM PowerPlex 16 PowerTyper Macro 2 0 www promega com geneticidtools GeneMappere ID Promega vi WwWw promega com geneticidtools panels_bins Matrix standard ABI PRISM 310 377 DNA PowerPlex Matrix Standards 310 ABI PRISM 3100 3100
82. 9 CTMD012 49 o Promega Penta E Panel B JOE D58818 D13S317 D7S820 D16S539 CSF1PO Penta D Panel C TMR Amelogenin vWA D8S1179 TPOX FGA Panel D ILS600 60bp 500bp A LAAL AMEN X jt ELEZLE e SI LIILILAZ tjejs sja ee sajast Bl LILILJ whe 5682 TA Figure 10 PowerPlex 16 Ladder Applied Biosystems 3130 3kV 5 PowerPlexe 16 Ladder GeneMapper ID 3 2 PowerPlex 16 Panel Bin Panel A FL Panel B JOE Panel C TMR STR
83. Amp PCR 9700 Start Select Method Options TRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 14 9600 Ramp Mode V V A ABI PRISM 3100 3100 Avant Version 2 0 Applied Biosystems 3130 z 3130xl Version 3 0 95 C EDK AIK IKEA WERKA 3100 3130 36cm 3100 3130 Performance Optimized Polymer 4 POP 4 EDTA 10x MicroAmp 35 96 Hi Di iti Applied Biosystems Cat 4311320 e PowerPlex Matrix Standards 3100 3130 Cat DG4650
84. D CSF1PO D16S539 D7S820 D13S317 D5S818 Penta E D18S51 D21S11 TH01 D3S1358 FL FGA TPOX D8S1179 vWA Amelogenin TMR Penta D CSF1PO D16S539 D7S820 D13S317 D5S818 6 4 5 2 7 JOE 16 PowerPlex 16 Monoplex Penta E Cat DC6591 Penta D JOE Cat DC6651 Penta E Penta D PowerPlex 16 PowerPlexe 16 BIOR PowerPlex 2 1 DNA DNA PowerPlex 16 ABIl PRISM 310 3100 3100 Avant Applied Biosystems 31304113130 x 18 424 Hr 1M LL RABI PRISM 377 DNA
85. D012 48 o Promega 1 2 9947A DNA DNA Ladder 9947A DNA Table 6 IX B 65 VI H PowerPlex 16 System Figure 9 PowerPlex 16 Allelic Ladder Mix Figure 10 A mo ELI ois puu mar T B ee eee NB 53 G a m SS my E D M me e we hl e Lad um t wt c vt t Figure 9 PowerPlex 16 System 1 Jl PowerPlex 16 10xPrimer Pair Mix DNA 1 0ng ILS600 Applied Biosystems3130 3kV 5 GeneMapper ID 3 2 Panel A FL D3S1358 TH01 D21S11 D18S51 TBA CERO 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 0
86. HID VI B 23 12 Edit delete size label auto adjust sizes ILS ILS600 ar BER o e Hafa Either panel size standard or analysis method is invalid C Classic k Basic or Advanced
87. TA BR QR Hub 2 4 MRA 6 95 C 3 3 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 16 CTMD012 o Promega Na We 2 0 Cversion 2 0 ABI PRISM 3100 3100 Avant 3130 3130x Module Manager New Type Regular Template HIDFragmentAnalysis36 POP4 5 seconds 3kV 2000 seconds OK Ea Module Manager
88. TRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 5 1x 300ul Gold STX R 10xBuffer 1x 250ul PowerPlex 16 10xPrimer Pair Mix 25ul 9947A DNA 10ng ul 1 x 25yl PowerPlex 16 Allelic Ladder Mix 1x 150ul Internal Lane Standard ILS 600 1 PowerPlex 16 System 400 DC6530 Cat DC6530 25HI 400 4 x 300ul Gold STXR 10xBuffer 4x 250ul PowerPlex 16 10xPrimer Pair Mix 25ul 9947A DNA 10ng ul 4 x 25yl PowerPlex 16 Allelic Ladder Mix 4x 150ul Internal Lane Standard ILS 600 1 PowerPlex 16 Ladder 36 B 907 909 010 5
89. able 4 Allele Table CODIS Table 2 2 Allele Table Categories CODIS Table Export to File Microsofte Excel PowerTable Format Sample Sample Peak Peak Peak Peak Over Low Satura Edited Edited Info Comment Category 1 2 3 4 flow Signal tion Label Row Allele Table Format CODIS Table Format Sample Info 11 File Save as PowerTyper Macro Genotypere Save Save as VLG XK 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTM
90. can VI F 45 GeneScan8 GeneScane Analysis Software User s Manual va ae TER 1 010 58256268 010 58256160 100013 3 09 44 36 B 907 909 www promega com cn CTMD012 Promega 2 3 VI F Genotyper PowerTyper 16 Macro
91. com Promega 5 PowerPlex16 advanced 6 Allele Figure 3 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 32 jo Anaby is Method ftor HID x Generi Allele Pom Deteeter Paak Des Quality Flags Wn Sot Frnegpi_ Di 10322 x 7 Use maler spaccfo siet aho 4 guaciable i Made Repeat Type Coto Value bo bo bo bo Mares Shutter Rato bo bo bo bo bo bo od THTI w LAI F TI TIT J Figure 3 Allele Bin Promega_Bins_1D3 2 X txt X Bin 7 Bin PowerPlex System Promega_Bins_ID3 2 X X Bin 8 Use marker specific stutter ratio if available 9 PowerPlexe 16 System Figure 2 Stutter Applied Biosystems nstal
92. der Macro Ladder 5 Casework POWER POWER Ladder Ladder Penta E D18S51 D21S11 THO1 D3S1358 POWER 20 Filter POWER POWER 20 Filter Ladder Table 5 IX B 65 36 B 907 909 010 58256268 010 58256160 1
93. displays only the 106 base X specific band TMR carboxy tetramethylrhodamine FL fluorescein JOE 6 carboxy 4 5 dichloro 2 7 dimethoxyfluorescein 36 B 907 909 010 58256268 010 58256160 100013 3 09 www promega com cn CTMD012 67 NA not applicable Table 5 PowerPlex 16 Ladder Size Range of Allelic Repeat Numbers of Ladder Components Repeat Numbers of Allelic Alleles Not Present STR Locus Label bases Ladder Components in Allelic Ladder Penta E FL 379 474 5 24 20 3 D18551 FL 290 366 8 10 10 2 11 13 132 14 27 D21511 FL 203 259 24 24 2 25 25 2 26 28 28 2 29 29 2 30 30 2 31 31 2 32 32 2 33 33 2 34 34 2 35 35 2 36 38 THOI FL 156 195 4 9 93 10 11 13 3 D351358 FL 115 147 12 20 FGA TMR 322 444 16 18 182 19 192 20 202 21 212 22 222 23 232 24 242 25 252 26 30 312 432 442 452 462 TPOX TMR 262 290 6 13 D851179 TMR 203 247 7 18 vWA TMR 123 171 10 22 Amelogeni TMR 106 112 X Y Penta D JOE 376 449 22 32 5 7 17 CSFIPO JOE 321 357 6 15 D165539 JOE 264 304 5 8 15 D75820 JOE 215 247 6 14 D135317 JOE 176 208 7 15 D55818 JOE 119 155 7 16 The length of each allele in the allelic ladder has been confirmed by sequence analyses When using an
94. hose sample s will not be shown Matrix fii Hj GeneMapper ID Matrix jenny Panel Bin Unable to panel save data java SQLEException ORA 00001 unique constraint IFA CKP NNN violated Ladder Ladder Panel Bin BUE GeneMapper ID Panel Bin GeneMapper ID HID GeneMapper fF GeneMapper ID GeneMapper Ladder Promega GeneMapper ID PowerPlex Jz Ni GeneMapper ID 3 1 3 2 36 B 907 909 010 58256268 3 09 010 58256160 100013 WWNW promega com cn
95. internal lane standard such as the Internal Lane Standard 600 the calculated sizes of allelic ladder components may differ from those listed This occurs because different sequences in allelic ladder and ILS components may cause differences in migration The dye label also affects migration of alleles 3 The alleles listed are those with a frequency of gt 1 1000 For a current list of microvariants see the Variant Allele Report published at PRA CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 68 o Promega the U S National Institute of Standards and Technology NIST web site at www cstl nist gov div831 strbase 2 Amelogenin is not an STR but displays a 106 base X specific band and a 112 base Y specific band Table 6 PowerPlex 16 DNA Standard DNA Templates STR Locus K562 9947A 99485 Penta E 14 5 13 12 11 11 D18S51 16 15 19 15 18 15 D21511 31 30 29 30 30 30 29 THO1 93 93 93 8 93 6 D3S1358 16 16 15 14 17 15 FGA 24 21 24 23 26 24 TPOX 9 8 8 8 9 8 D851179 12 12 13 13 13 12 vWA 16 16 18 17 17 17 Amelogenin X XA X Y Penta D 13 9 12 12 12 8 CSFIPO 10 9 12 10 12 11 10 D165539 12 11 12 11 11 11 D75820 11 9 11 10 11 11 D135317 8 8 11 1
96. lation Procedures and New Features for GeneMapper ID Software 3 2 36 Huts B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 33 CTMD012 o Promega Applied Biosystems 10 Peak Detector Figure 3 full range partial range 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 34 ay et bad Editor HID Genara Attala Pest Detector Pom Quant Ovasta Flog Pom Deteten FF Range Poem Cab tes Fem Amplinnss Thwshelds a te Shp Sinbo x m y v
97. o Promega Part CTMD012 PowerPlex 16 System DC6530 DC6531 TMD012 HKZ CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 0 PowerPlex 16 www promega com tbs Promega E mail genetic promega com RC 3 Me occnsccodcccacceacecctenccucedeccsnedetsactcnsesnasecdacsaccecvecetenecusaeevs 5 IREE EIDA 7 7 B Matrix 9 IV PowerPlex 16 DNA pe 9 A 10 B 13 VA a E 15 A ABI PRISM 3100 3100 Avant Version 2 0 Applied Biosystems 3130 3130xl Version 3 0
98. omega com Promega 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 4 Amplification Setup Section IV A Thermal Cycling Section IV B GeneAmp PCR System 9700 GeneAmpe PCR System 9600 GeneAmp PCR System 2400 Model 480 Thermal Cycler Instrument Setup and Sample Preparation Section V Applied Biosystems 3130 ABI PRISM 3100 or 3100 ABI PRISMe 3100 Genetic or 3130x Genetic Analyzer Avant Genetic Analyzer with Analyzer with Data Collection with Data Collection Data Collection Software Software Version 1 0 1 or 1 1 Software Version 3 0 Version 2 0 Section V B Section V A Section V A ABI PRISM 310 Genetic ABI PRISM 377 DNA Analyzer Sequencer Section V C Section V D Data Analysis Section VI GeneMapper D Software GeneScan Software and GeneScan Software and Versions 3 1 and 3 2 PC Operating Systems Macintosh Operating Systems Figure 1 PowerPlex 16 System ll PowerPlex 16 System 100 DC6531 Cat DC6531 25HI 100
99. p 23 to 70 C for 45 seconds for 22 cycles then for 22 cycles then 60 C for 30 minutes 60 C for 30 minutes 4 C soak 4 C soak Protocol for the GeneAmp PCR Protocol for the Perkin Elmer System 9600 Thermal Cycler Model 480 Thermal Cycler 95 C for 11 minutes then 99 C for 11 minutes then 96 C for 1 minute then 96 C for 2 minutes then 94 C for 30 seconds 94 C for 1 minute ramp 68 seconds to 60 C hold for 30 seconds 60 C for 1 minute ramp 50 seconds to 70 C hold for 45 seconds 70 C for 1 5 minutes for 10 cycles then for 10 cycles then 90 C for 30 seconds 90 C for 1 minute ramp 60 seconds to 60 C hold for 30 seconds 60 C for 1 minute ramp 50 seconds to 70 C hold for 45 seconds 70 C for 1 5 minutes for 22 cycles then for 22 cycles then 60 C for 30 minutes 60 C for 30 minutes 4 C soak 4 C soak 143847 GeneAmp PCR 9700 9600 Ramp Mode Ramp Rate Modification More Modify Ramp Rate Modification Ramp Rate Modification 100 Figure 2 Gene
100. ucts may be covered by pending or issued patents or may have certain limitations Please visit our Web site for more information All prices and specifications are subject to change without prior notice Product claims are subject to change Please contact Promega Technical Services or access the Promega online catalog for the most up to date information on Promega products 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 80
101. vWA 6 21 VIl JOE D5S818 114 120bp Low Level Artifact TMR 142 144bp 400 405bp Vi Promega www promega com E mail Chinatech promega com
102. xwell Plexor and PowerPlex are registered trademarks of Promega Corporation Differex DNA IQ PowerTyper and Slicprep are trademarks of Promega Corporation ABI PRISM GeneMapper GeneScan Genotyper and MicroAmp are registered trademarks of Applera Corporation AmpliTaq Gold and GeneAmp are registered trademarks of Roche Molecular Systems Inc ART is a registered trademark of Molecular Bio Products Inc Biomek is a registered trademark of Beckman Coulter Inc Freedom EVO is a registered trademark of Tecan AG Corporation FTA is a registered trademark of Flinders Technologies Pty Ltd and is licensed to Whatman GenBank is a registered trademark of the U S Dept of Health and Human Services Hi Di and POP 4 are trademarks of Applera Corporation Liqui Nox is a registered trademark of Alconox Inc Long Ranger and Long Ranger Singel are registered trademarks of Cambrex Corporation Macintosh is a registered trademark of Apple Computer Inc Microsoft Windows and Windows NT are registered trademarks of Microsoft Corporation Nalgene is a registered trademark of Nalge Nunc International Triton is a registered trademark of Union Carbide Chemicals and Plastics Technology Corporation TAZ CIEE 36 B 907 909 010 58256268 010 58256160 100013 www promega com cn 3 09 CTMD012 79 o Promega Prod

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